Objective To investigate the effects of nicardipine on apoptosis in ischemia?reperfusion myocardium of rabbits. Methods Forty New Zealand rabbit were randomly divided into 4 groups:control group(group A),ischemia group(group B),ischemia?reperfusion group(group C),ni?cardipine treatment group(group D). Ischemia?reperfusion model was established by left circumflex branch coronary artery ligation for 40 min,fol?lowed by 120 min reperfusion. Apoptotic cardiomyocyte was detected using the analysis for the exposure of phosphatidyl?serine ( Annexin V)meth?od. Results Myocardial cell damage was lower in D group than that in group B and group C from pathology(P<0.01). The myocardial cell apopto?sis in group D is less than that of group B and group C(P<0.01),but higher than that of group A(P<0.05). Myocardial cell necrosis is lower than group B and group C(P<0.01),and no difference was found comparing with group A(P>0.05). Conclusion Nicardipine horizon can reduce the myocardial cell apoptosis in rabbits with myocardial ischemic reperfusion injury.

Objective: To observe the clinical efficacy of tuina in treating depression.Method: Eighty depression cases that conformed to the inclusive criteria were randomized into tuina and Deanxit group, with 40 cases in each group. Tuina manipulations were adopted for cases in the tuina group, 30 min for each treatment. While 2 Deanxit tablets were adopted for cases in the Deanxit group, twice a day. The clinical efficacy of the two groups was evaluated by using Hamilton Rating Scale for Depression (HAMD) before treatment and the 2nd, 4th and 6thweeks after. In addition, the Treatment Emergent Symptom Scale (TESS) was adopted to evaluate the adverse reactions in the two groups. Result: Both tuina and Deanxit can help to relieve the symptoms of depression patients. The effective rate of tuina and Deanxit were 72.5% and 70.0% respectively. However, there were no adverse reactions in tuina group, while 12.5% of adverse reaction rate occurred in the Deanxit group. Conclusion: Tuina can definitely benefit patients with depression and cause no adverse reactions.

Objective To introduce a new method for correction of epicanthal folds, which could avoid obvious scar in medial canthus. Methods First, through upper and lower palpebral margin incisions, the connective and orbicularis oculi muscle dense fibers underlying epicanthal folds as well as the superficial head of medial canthal ligament were thoroughly released and excised. After repositioning the medial canthus, the redundant skin tissue was removed and then the incisions closed. Results 122 epicanthoplasties had been performed. Postsurgical scars were invisible in all cases. All patients were satisfied with operation results. No recurrence of epicanthal fold was observed. Conclusions The introduced method has advantages in avoiding scar formation and is an especially suitable approach for epicanthoplasty in Asian patients.

Objective To study the prevention and treatment of intra- or post- operative complications of radiofrequency ablation (RFA) for hepatic malignancy. Methods A series of 353 patients with primary hepatic carcinoma or secondary hepatic malignancy verified by pathological examinations and clinical manifestations underwent 452 times of RFA between October 1999 and March 2003, including 344 cases of percutaneous RFA and 9 cases of RFA during open surgery. There were 198 cases of primary hepatic carcinoma previously unoperated, 81 cases of recurrent hepatic carcinoma after surgery, and 74 cases of secondary hepatic malignancy. All the patients were followed up to make out short- or long-term surgical complications. Results Complications happened in 12 cases: skin burn by the electrode (2 cases), pneumothorax (1 case), right hydrothorax (1 case), vena cava thrombus (1 case), right bile duct injury and stricture (1 case), gastric external fistula (1 case), bile leakage in the left lateral lobe of the liver (1 case), hepatic abscess (1 case), external fistula of the ascending colon resulted from hepatic abscess (1 case), internal fistula of the duodenum, liver and right thorax resulted from hepatic abscess (1 case), and intra-abdominal hemorrhage (1 case). The incidence of complication was 2 65% (12/452) and the complication-related mortality was 8 33% (1/12). Conclusions RFA is a kind of minimally invasive procedure. But RFA especially percutaneous RFA may involve some risks for patients with the tumor located at the porta hepatic or the surface of the liver, or adhered to hollow organs, especially those who had a history of abdominal operation and adhesions of hollow viscera to the liver. Some complications can be prevented and effectively treated in the event of proper selection of patients, careful performance during the surgery, intensive postoperative care, effective hemostasia and efficient infection prevention.

Activities of N-acetyl-?-glucosaminidase (NAG) in sera of normal subjects and patients with hepatocellular carcinoma (HCC) and other diseases were microquantitatively determined by spectrophotometry. The results showed that after the reaction for 4 h, activity of serum NAG in patients with HCC (397.10 ? 174.97 nmol/ml) was obviously higher than that in normal subjects (280.00 ? 63.83 nmol/ml). The elevation of serum NAG in HCC patients had no relationship with their serum a-fetoprotein. However, the increase of serum NAG activity in some patients with benign liver diseases was always accompanied by the abnormality in their liver functions. It is suggested that serum NAG has some referable values in the diagnosis of HCC.

Objective To investigate the effect of lovastatin on hyperlipemia of children with nephrotic syndrom.Methods 43 cases of nephrotic syndrome were randomly divided into treatment group (n=21) and control group (n=22), all of which were given prednisone.Lovastatin was added to the treatment group.Before treatment and one month after treatment liver function and serum lipide were measured.Results The levels of serum apolipoprotein B (ApoB),total cholesterol (TC), trigtycerides (TG), low density lipoprotein cholesterol (LDL C) and lipoprotein (a) (Lpa) were obviously decreased compared with that before treament (P

ve To establish a method of high performance liquid chromatography (HPLC) for determi-nation of nitrite in water. Methods The trace content of nitrite in water sample was determined by indirect UV-HPLC. The mixture of methanol/o-phthalic acid (pH value was adjusted to 8.6 by 0.10 mol/L NaOH solution)5: 95 was defined as mobile phase. The water sample was directly filtered by chromatographic column pack with ODP. Results Under the conditions of wave length of 270 nm and flow rate of 0.9 ml/ min, the linear range of this assay was 0.0~20.0?g/ml nitrite, the relative standard deviation was 3.9%. The average recovery rate and detection limit were 99.9% and 0.001?g/ ml respectively. Conclusion This method could be applied to the determination of trace amount of nitrite in drinking water, purified water and mineral water.

Objective To verify the applicable significance of confocal scanning laser retinal tomography in age-related macular degeneration (AMD). Methods Heidelberg confocal scanning laser tomography was used to measure 75 eyes of 59 patients with AMD, including 25 eyes of 20 patients with exudative AMD, 25 eyes of 16 patients with atrophic AMD , and 25 eyes of 23 patients with macular drusen. The differences of the width, volume and maximum height of Z profile signal of macula were analyzed. Results Z profile signal width in macular tomography of exudative AMD was wider than that of macular drusen; maximum height and volume in macular tomography of exudative AMD were larger than that of macular drusen (P

OBJECTIVE: To develop a gradient supercritical fluid chromatography method for the separation of cinnarizine and its four related substances. METHODS: Cinnarizine and its four related substances were separated on a Torus DIOL column (3.0 mm×100 mm,1.7 μm) maintained at 40 ℃ with the mobile phase consisting of CO2 and methanol with 0.1% TFA-0.1% TEA at 1.5 mL•min-1, the detection wavelength was set at 230 nm and the back pressure was set at 1.38×107 Pa. RESULTS: Cinnarizine and its four related substances were separated in 4.5 min with satisfying resolutions. Good linear relationships were established between the peak response and the concentration in the range of 2-20 μg•mL-1 for each related substance (r>0.999 9) and the detection limits were 0.7-1.3 ng(S/N≥3). Good linear relationships were established between the peak response and the concentration in the range of 0.05-1.0 μg•mL-1 for cinnarizine (r=1.000 0). The spiked recovery of four related substances of cinnarizine was 98.0%-106.7%, and the RSD was 2.57%-4.44%(n=9). CONCLUSION: The established method can be applied in the simultaneous determination of the related substances of cinnarizine and provide reference for the quality control.

Objective To observe the effect of compound traditional Chinese drug on nerve conduction velocity and electromyography in diabetes mellitus patients with diabetic peripheral neuropathy.Method Fifty-seven patients were randomly divided into tow groups.The control group(27 patients)were treated with Vitamin B,and the test group (30 patients)were treated with compound traditional Chinese drug.The never conduction vielocity and electromyography were measured before and after the treatment.Results In the test group,the nerve conductive vielocity was increased and the spontaneous electric potential of electromyography was decreased;whereas,no obvious changes were found in the control group after treatment,and there was significant difference between the two groups(P