1.Application of reverse transcription loop-mediated isothermal amplification technology in the field of oncology
Cancer Research and Clinic 2013;25(7):493-495
Reverse transcription loop-mediated isothermal amplification(RT-LAMP)is a novel nucleic acid amplification technique which has been mainly used in a variety of viruses and other pathogens of gene detection.Compared with reverse transcriptase polymerase chain reaction (RT-PCR),the RT-LAMP has advantages of reaction simplicity,rapidly,cost-effective,detection sensitivity and specificity.Nowadays,RT-LAMP technology is gradually used in various medical fields,but it is still a new method especially in cancer diagnosis of tumor gene.The article reviews the application of the RT-LAMP technology in the cancer
2.Mechanisms of stem cells involved in gastric cancer
Journal of International Oncology 2010;37(12):928-930
Researches indicate that gastric carcinoma occurs where gastric stem cells settle down.Stem cells may play an important role in formative process of gastric carcinoma. Researches on relationship between stem cells and gastric stem cells, tumor stem cells and stomach neoplasms, bone marrow stem cells and stomach neoplasms indicate that gastric carcinoma may be a kind of stem cell diseases.
3.Research on aberrant expression of HOX in gastrointestinal neoplasms and its mechanism
International Journal of Surgery 2012;39(6):396-399
Homebox genes are known to determine cell proliferation and differentiation and subsequently regulate the occurrence,development and prognosis of many tyhpes of malignant tumors in human.Some recent studies have shown that homebox genes are abnormally expressed in the esophageal,gastric,coloreetal and other gastrointestinal tumors.In this review,we consider that homebox genes may regulate the occurrence,development of gastrointestinal tumors by interacting with transcription factors in vivo and abnormal epigenetic modifications.Therefore,homebox genes are closely related to gastrointestinal tumors.
4.An Experimental Study on the Biocompability of Porcelain Compounded With Nanostructured Material
Journal of China Medical University 2001;30(2):105-107
Objective: Our aim was to evaluate the biocompability of porcelain compounded with nanostructured material. Methods:The proliferation of the NIH3T3 cells were investigated using a tetrazolium-based colorimetric assay(MTT assay).Maceration extract of four tested materials were added into culture plate .The samples were tested with ultraviolet spectrophotometer in 490 wavelength at 1,3,5,7 days after the addition of extract. Morphology of these cells was also observed with phase-contrast microscope. Results: There was no obvious difference among three kinds of prosthetic materials in the optical absorbance and the cells growing in the extract showed normal morphology. Conclusion: The toxicity graduation of the porcelain compounded with nanostructured material is 0. This material has a good biocompability and can be used clinically.
5.Application of Repetitive Transcranial Magnetic Stimulation for Neuropathic Pain (review)
Chinese Journal of Rehabilitation Theory and Practice 2016;22(7):809-812
Repetitive transcranial magnetic stimulation (rTMS) may release neuropathic pain (NeP) by changing the excitability of cor-tex, improving the cerebral blood flow, regulating the expression of neurotransmitter, fitting the plasticity, reducing the overexpression of neuronal nitric oxide synthase and overaction of astrocyte, etc. It is used for management of post-herpetic neuralgia, thalamic pain after stroke, NeP after spinal cord injury, phantom limb pain, atypical facial pain post operation of trigeminal nerve, etc. The outcome is associat-ed with the location, frequency, pulses, intensity of stimulation, as well as the shape of the stimulator, etc.
6.Diagnosis strategy of Guillain-Barré syndrome spectrum disorders
Chinese Journal of Applied Clinical Pediatrics 2016;31(12):884-886
Guillain-Barré syndrome(GBS) has clinical characteristics:flaccid,symmetrical,ascending paralysis.Cranial nerves and respiratory muscle related,albuminocytologic dissociation in cerebrospinal fluid,and electrophysiological changes.GBS was believed to be an autoimmune perineuropathy.Recently,there were more and more reports about GBS spectrum disorders or GBS variant correlated with anti-GQ1b antibody or anti-GM1 IgG antibody et al.The GBS Classification Group presented the new clinical criteria in 2014,to enable neurologists and non-neurologists to diagnose GBS and all its variants using a simple yet all-inclusive classification system.
7.Therapeutic Effect of Acupuncture plus Tuina on Cervical Spondylosis
Journal of Acupuncture and Tuina Science 2008;6(6):344-346
Objective: To observe the clinical effect of acupuncture plus tuina on cervical Spondylosis. Method: Altogether 146 cases were randomized into a group of acupuncture plus tuina (observation group) (70 cases), an acupuncture group (control group I) (38 cases) and a tuina group (control group II) (38 cases). Result: The recovery rate in the observation group, control group I and control group II were 92.1%, 68.4% and 65.8% respectively; there was a significant statistical difference between the observation group and two control groups (P<0.01); and there was no significant statistical difference between the two control groups (P>0.05). Conclusion: Acupuncture plus tuina can alleviate the patients' condition in a faster and better way.
8.Expressions of SOCS3 and IL-6 in Triple-negative Breast Cancer and Their Chinical Significance
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong 2015;(1):82-86
Objective To investigate the expressions of SOCS3 and IL‐6 in triple‐negative breast cancer(TNBC)and their clinical significance.Methods The expressions of SOCS3 and IL‐6 in TNBC and normal breast cells were detected by Western blot and fluorescence quantitative PCR.Immunohistochemical staining was used to detect their expressions in 84 TNBCs ,33 breast fibromas and 11 normal breast tissues. The correlation of their expressions in TNBC and the relationship between their expressions and clinical pathological parameters were analyzed as well.Results SOCS3 was not expressed or down‐expressed in TNBC cell lines while IL‐6 was over‐expressed. The positive rate of SOCS3 protein was 32.1% in TNBCs ,63.6% in breast fi‐bromas(P<0.01)and 72.7% in normal breast tissues (P< 0.01);and that of IL‐6 protein was 72.6% in TNBCs ,30.3% in breast fibromas(P< 0.01)and 27.3% in normal breast tissues (P< 0.01).Moreover ,a significant negative correlation was found between the expression of SOCS3 and that of IL‐6 in TNBC(rs = -0.590 ,P<0.01). The expressions of SOCS3 and IL‐6 were markedly associated with tumor size ,lymph node metastasis ,clinical stages and expressions of Ki‐67 and p53(P<0.05) , but not with age ,histological grades or menopausal status(P>0.05).Conclusion The expressions of SOCS3 and IL‐6 are asso‐ciated with the development of TNBC ,suggesting that SOCS3 and IL‐6 can serve as biological markers for evaluating the prog‐nosis ,and therapeutic targets of T NBC.
9.Bioabsorbable pins improve bone healing of juvenile unstable osteochondritis dissecans
Chinese Journal of Tissue Engineering Research 2016;20(8):1133-1139
BACKGROUND: At present, there are different strategies for treating unsteable osteochondritis dissecans of the knee joint in adolescents, but their therapeutic effects are not ideal. OBJECTIVE: To assess the therapeutic effect of bioabsorbable pin fixation on functional recovery and fracture healing in juvenile unstable osteochondritis dissecans through Lysholm, IKDC, Hughston scoring and imaging examination. METHODS: Eighteen patients with unstable osteochondritis dissecans were enrol ed. Under arthroscopy, a smal incision was localized and cut fol owed by bioabsorbable pin fixation. The average fol ow-up period was 2.7 years. Functional recovery of the affected limbs after fixation was assessed by using Lysholm, IKDC and Hughston scoring. X-ray examination was used to observe fracture healing. RESULTS AND CONCLUSION: At 6 months after fixation, the Lysholm and IKDC scores were improved significantly as compared with those before operation and maintained til the last fol owing up. There were 15 patients scored as excel ent, 2 as good and 1 as poor. X-ray films also showed that there were 17 cases of fracture healing (94% healing rate). The average fracture healing time was 2.9 months. These findings indicate that the bioabsorbable pins can improve the bone healing in juvenile unstable osteochondritis dissecans.
10.The tests and correlation analysis of HBV-DNA and hepatic fibrosis indicators in chronic hepatitis B patients
International Journal of Laboratory Medicine 2014;(17):2311-2312
Objective To explore the correlation between serum HBV-DNA content and hepatic fibrosis indicators in hchronic epatitis B patients.Methods Serum HBV-DNA content was determined by using real-time fluorescence quantitative PCR and he-patic fibrosis indicators including hyaluronic acid,laminin,procollagen Ⅲ,and collagen Ⅳ which were determined by using chemilu-minescence.Results The logarithm value of serum HBV-DNA content of the 110 hepatitis B patients was 5.32±1.37.Serum hyal-uronic acid concentration was (197.81 ±85.37)mg/mL;procollagen Ⅲ concentration was (142.66 ±30.28)μg/mL.Collagen Ⅳconcentration was (90.34 ±20.53)μg/mL.Laminin was (143.96 ±31.79 )μg/mL.All these indicators were significantly higher than the control group (P <0.01).But there were no significant linear correlation between serum HBV-DNA content and the four hepatic fibrosis indicators(P >0.05).Conclusion The hepatic fibrosis indicators increased significantly in hepatitis B patients but there were no significant correlations between serum HBV-DNA content and the hepatic fibrosis indicators.