Researches indicate that gastric carcinoma occurs where gastric stem cells settle down.Stem cells may play an important role in formative process of gastric carcinoma. Researches on relationship between stem cells and gastric stem cells, tumor stem cells and stomach neoplasms, bone marrow stem cells and stomach neoplasms indicate that gastric carcinoma may be a kind of stem cell diseases.

Reverse transcription loop-mediated isothermal amplification(RT-LAMP)is a novel nucleic acid amplification technique which has been mainly used in a variety of viruses and other pathogens of gene detection.Compared with reverse transcriptase polymerase chain reaction (RT-PCR),the RT-LAMP has advantages of reaction simplicity,rapidly,cost-effective,detection sensitivity and specificity.Nowadays,RT-LAMP technology is gradually used in various medical fields,but it is still a new method especially in cancer diagnosis of tumor gene.The article reviews the application of the RT-LAMP technology in the cancer

BACKGROUND: At present, there are different strategies for treating unsteable osteochondritis dissecans of the knee joint in adolescents, but their therapeutic effects are not ideal. OBJECTIVE: To assess the therapeutic effect of bioabsorbable pin fixation on functional recovery and fracture healing in juvenile unstable osteochondritis dissecans through Lysholm, IKDC, Hughston scoring and imaging examination. METHODS: Eighteen patients with unstable osteochondritis dissecans were enrol ed. Under arthroscopy, a smal incision was localized and cut fol owed by bioabsorbable pin fixation. The average fol ow-up period was 2.7 years. Functional recovery of the affected limbs after fixation was assessed by using Lysholm, IKDC and Hughston scoring. X-ray examination was used to observe fracture healing. RESULTS AND CONCLUSION: At 6 months after fixation, the Lysholm and IKDC scores were improved significantly as compared with those before operation and maintained til the last fol owing up. There were 15 patients scored as excel ent, 2 as good and 1 as poor. X-ray films also showed that there were 17 cases of fracture healing (94% healing rate). The average fracture healing time was 2.9 months. These findings indicate that the bioabsorbable pins can improve the bone healing in juvenile unstable osteochondritis dissecans.

Repetitive transcranial magnetic stimulation (rTMS) may release neuropathic pain (NeP) by changing the excitability of cor-tex, improving the cerebral blood flow, regulating the expression of neurotransmitter, fitting the plasticity, reducing the overexpression of neuronal nitric oxide synthase and overaction of astrocyte, etc. It is used for management of post-herpetic neuralgia, thalamic pain after stroke, NeP after spinal cord injury, phantom limb pain, atypical facial pain post operation of trigeminal nerve, etc. The outcome is associat-ed with the location, frequency, pulses, intensity of stimulation, as well as the shape of the stimulator, etc.

Objective To explore the establishment methods of animal models of intestinal multidrug-resistant bacterial (Pseudomonas aeruginosa, PA) infection, and to provide a good model for experimental research and treatment for infection caused by drug-resistant bacteria .Methods Male BALB/c mice were divided into four groups:Normal con-trol group, MDR-PA group, MDR-PA +antibiotics group, and MDR-PA +fasting group.They were given saline, PA suspension, PA suspension after free drinking with ceftriaxone water and PA suspension after fasting , respectively , by ga-vage.Colon tissues were collected for pathological examination and inflammation scoring .The expressions of TNF-αand INF-γin the colon tissue was assessed at the end of the experiment .Results Pathology showed different degrees of inflam-matory changes in the colon tissues .Compared with the normal control group , the expressions of TNF-αand INF-γhad sig-nificant differences with that of experimental groups .Conclusions All the three methods used in this experiment can be successfully used to establish mouse models with intestinal infection caused by multidrug-resistant Pseudomonas aeruginosa, and may be chosen for experimental studies according to different research purposes .

10.3969/j.issn.2095-4344.2013.25.026

Objective To investigate the expressions of SOCS3 and IL‐6 in triple‐negative breast cancer(TNBC)and their clinical significance.Methods The expressions of SOCS3 and IL‐6 in TNBC and normal breast cells were detected by Western blot and fluorescence quantitative PCR.Immunohistochemical staining was used to detect their expressions in 84 TNBCs ,33 breast fibromas and 11 normal breast tissues. The correlation of their expressions in TNBC and the relationship between their expressions and clinical pathological parameters were analyzed as well.Results SOCS3 was not expressed or down‐expressed in TNBC cell lines while IL‐6 was over‐expressed. The positive rate of SOCS3 protein was 32.1% in TNBCs ,63.6% in breast fi‐bromas(P<0.01)and 72.7% in normal breast tissues (P< 0.01);and that of IL‐6 protein was 72.6% in TNBCs ,30.3% in breast fibromas(P< 0.01)and 27.3% in normal breast tissues (P< 0.01).Moreover ,a significant negative correlation was found between the expression of SOCS3 and that of IL‐6 in TNBC(rs = -0.590 ,P<0.01). The expressions of SOCS3 and IL‐6 were markedly associated with tumor size ,lymph node metastasis ,clinical stages and expressions of Ki‐67 and p53(P<0.05) , but not with age ,histological grades or menopausal status(P>0.05).Conclusion The expressions of SOCS3 and IL‐6 are asso‐ciated with the development of TNBC ,suggesting that SOCS3 and IL‐6 can serve as biological markers for evaluating the prog‐nosis ,and therapeutic targets of T NBC.

Objective: To observe the clinical effect of acupuncture plus tuina on cervical Spondylosis. Method: Altogether 146 cases were randomized into a group of acupuncture plus tuina (observation group) (70 cases), an acupuncture group (control group I) (38 cases) and a tuina group (control group II) (38 cases). Result: The recovery rate in the observation group, control group I and control group II were 92.1%, 68.4% and 65.8% respectively; there was a significant statistical difference between the observation group and two control groups (P<0.01); and there was no significant statistical difference between the two control groups (P>0.05). Conclusion: Acupuncture plus tuina can alleviate the patients' condition in a faster and better way.

Objective To explore the correlation between serum HBV-DNA content and hepatic fibrosis indicators in hchronic epatitis B patients.Methods Serum HBV-DNA content was determined by using real-time fluorescence quantitative PCR and he-patic fibrosis indicators including hyaluronic acid,laminin,procollagen Ⅲ,and collagen Ⅳ which were determined by using chemilu-minescence.Results The logarithm value of serum HBV-DNA content of the 110 hepatitis B patients was 5.32±1.37.Serum hyal-uronic acid concentration was (197.81 ±85.37)mg/mL;procollagen Ⅲ concentration was (142.66 ±30.28)μg/mL.Collagen Ⅳconcentration was (90.34 ±20.53)μg/mL.Laminin was (143.96 ±31.79 )μg/mL.All these indicators were significantly higher than the control group (P <0.01).But there were no significant linear correlation between serum HBV-DNA content and the four hepatic fibrosis indicators(P >0.05).Conclusion The hepatic fibrosis indicators increased significantly in hepatitis B patients but there were no significant correlations between serum HBV-DNA content and the hepatic fibrosis indicators.

ObjectiveTo explore the roles of Toll-like receptor (TLR) 3,7 and 9 in the pathogenesis of pityriasis rosea.MethodsThis study recruited 40 patients with pityriasis rosea and 40 normal human controls.Reverse transcription-PCR was carried out to detect the expressions of TLR3,7 and 9 in peripheral blood lymphocytes of these subjects.ResultsThe mRNA expressions of TLR3,7 and 9 in peripheral blood lymphocytes were significantly higher in patients with pityriasis rosea than in the normal human controls (Z =4.239,3.745,2.658,all P ＜ 0.05).There was a positive correlation between the expression of TLR3 and TLR7(r =0.574,P ＜ 0.01 ) as well as between that of TLR3 and TLR9 (r =0.551,P ＜ 0.01 ) in the patients,while there was no correlation between TLR7 and TLR9 expression in the patients or between the expressions of TLR3,TLR7 and TLR9 in the controls.ConclusionVirus infection might be involved in the pathogenesis of pityriasis rosea via TLR pathways.