Aim To investigate the effects of com-bined treatment of SAHA and TRAIL on human breast cancer ER positive cell line MCF-7 . Methods MCF-7 cells were treated with SAHA and/or TRAIL. The inhibitory rates were detected by real-time cell prolifer-ation assays. Morphology changes of MCF-7 cells were observed through time-lapse live cell imaging acquisi-tion. Results Real-time cell proliferation assays showed that the anti-tumor efficacy of SAHA was sig-nificantly enhanced in combination with TRAIL. The results of time-lapse live cell imaging acquisition dem-onstrated that, with treatment of SAHA and TRAIL, the growth inhibition of MCF-7 cells was more obvious than that of in TRAIL or SAHA treatment alone. Con-clusion The combination treatment of SAHA and TRAIL has a synergistic effect of growth inhibition on breast cancer MCF-7 cells.

Objective To analyze the effects of gentongping pill combined with physiotherapy on the patients with vertebral artery type of cervical spondylosis. Methods 134 patients with vertebral artery type of cervical spondylosis were grouped into two groups, the observation group and the control group. The two groups were treated with physiotherapy and the observation group was treated with Gentongping pill. The effects in the two groups were evaluated. Results The total efficiency in the observation group was higher than that in the control group(P<0.05). After treatment, the symptom score, CASCS score, mean blood flow velocity in left vertebral artery and right vertebral artery, TNF-α and IL-6 level in the observation group were better than those in the control group (all P<0.05). There was no obvious adverse reaction in the two groups. Conclusion Itcan improve the therapeutic effect which Gentongping pill is used on the treatment of patients with vertebral artery type of cervical spondylosis.

The medical equipment benefit evaluation analysis has become an important constituent of cost and medical equipment management. It can not only use the economic benefit index to estimate the service condition of equipment, but also form the comprehensive evaluation system with basic of economic benefit evaluation and auxiliary of science study. Based on comprehensive evaluation result, to search the weakness of equipment utilization and promote the overall development of hospitals and clinics orderly.

There are so many patients with different liver diseases in our country that the diagnosis and treatment of liver diseases directly relate to the people's healthy level.The requirement of diagnosis and treatment to clinical laboratories can be summarized as: ( 1 ) sensitivity,which means shortening window period;(2) specificity,which means various subtypes and variant can be detected;( 3 )speed,which means finding out the pathogen as soon as possible;(4)accuracy,which means providing physicians with accurate test reports.Detecting techniques,for instances,chemiluminescence,polymerase chain reaction and gene mutational site detecting method,were applied to the diagnosis and treatment of liver disease,thus improving the degree of precision,accuracy,sensitivity,specificity and detection speed and satisfying the requirement of diagnosis and treatment maximatily.

ObjectiveTo study the change of serum interferon- γ(IFN- γ ) and interleukin-4(IL-4)level in children suffered from cough variant asthma after montelukast combined with loratadine therapy.MethodsForty-six patients of children cough variant asthma were recruited and accepted montelukast combined with loratadine therapy for 8 weeks.The changes of serum IFN-γ and IL-4 before and after therapy were compared.ResultsCompared with before therapy,the level of serum IFN- γwas decreased [ (59.94 ± 9.43) ng/L vs.(39.94 ± 9.07) ng/L ] and IL-4 was increased[ ( 48.78 ± 22.23 ) ng/L vs.(60.78 ±16.48) ng/L] after therapy,there were significant differences (P ＜0.05).ConclusionMontelukast combined with loratadine in the treatment of children cough variant asthma can improve the balance of IFN- γ/IL-4,which deserve further study.

HepG2 cells were incubated with dexamethasone,glucocorticoid receptor (GR) antagonist RU486,and pioglitazone for 24 or 48 h.Glucose concentration in culture medium was detected.The results showed that 10 μmol/L RU486 blocked the surge of glucose concentration by 48 h of dexamethasone treatment and the glucose concentration in RU486 + pioglitazone group was higher than pioglitazone group( P＜0.05 ),suggesting that the cross talk between PPARγ and GR may exist in hepatic glucose metabolism.

10.3969/j.issn.2095-4344.2013.25.026

To explore the feasibility of sevoflurane used in obstetric anesthesia and examine its effects on neonatal Apgar scores.A total of 60 cases of term primipara were assigned into epidural anesthesia assisted sevoflurane inhalation group (experimental group,n =30) and epidural anesthesia group (control group,n =30).In the experimental group after skin incision,the patients could not tolerate the anesthesia mask inhalation of sevoflurane.And the control group received no adjuvant drug.The maternal inhalation of sevoflurane to delivery of fetus after 1 min,5 min,10 min Apgar scores were analyzed.And the inhalation of sevoflurane to fetal childbirth duration were 4-10 min in two groups.The intergroup difference of newborn Apgar scores had no statistical significance (P ＞ 0.05).Epidural anesthesia assisted sevoflurane inhalation for cesarean section had no significant effect on neonatal Apgar scores.

With the development of proteomic technologies, large numbers of putative biomarkers have been screened out but few of them are put into the clinical application.Recently, MRM-MS based approaches and strategies have aroused extensive concern in domestic and overseas clinical laboratory community because of their cost-efficiency, time-saving, high analytical sensitivity and accuracy.MRM-MS based approaches, especially SISCAPA, may gradually become a key technology for the protein biomarker research in the near future.In this review, the bottleneck existing in the field of protein biomarkers research, and features as well as applications of MRM-MS are discussed and the future trend of research is prospected.

Objective To explore the correlation between the depression status of patients with post-stroke depression(PSD) and nerve function defect.Methods 75 patients in the first 24h of acute stroke onset were selected.According to the Zung depression self rating scale(SDS),75 cases were divided into simple stroke patients group and PSD group,and the Hamilton depression scale (HAMD) and Scandinavian nerve function defect rating scale (SSS) were used for further evaluation.The score results were statistically analyzed.Results Of 75 cases with acute stroke,32 cases(42.7％) were diagnosed PSD.Admission day 2,at the end of 1,2 and 3 weeks,the HAMD scores between PSD patients and simple acute stroke group had statistically significant differences (all P ＜ 0.01).The HAMD scores of three groups of PSD patients (light,medium,heavy) appeared obvious changes with the prolonged time,which were significantly higher than the simple stroke group.The SSS score of simple stroke group and mild PSD patients increased with prolonged observation time,the difference between the two groups was significant (q =9.75,P ＜ 0.05).The SSS score of moderate and severe PSD patients decreased with prolonged observation time,compared with the simple stroke group,the differences were statistically significant(q =50.23,46.82,all P ＜ 0.05).In simple stroke patients and mild PSD patients,HAMD scores and SSS score had no correlation (r =0.062,P ＞ 0.05).In moderate and severe PSD patients,HAMD score and SSS score had correlation (r =4.682,P ＜ 0.05).Conclusion The incidence rate of PSD in acute brain stroke patients was 42.7％,and in patients with moderate and severe PSD,HAMD scores correlated with the degree of nerve function defect.Early intervention on depression treatment and promote the recovery of neural function is also important.