Underactive bladder is the main part of lower urinary tract symptoms. As a conservative treatment, intravesical electrical stimulation can improve the function of bladder sensation, and promote micturition. Intravesical electrical stimulation is effective and safe on underactive bladder, both idiopathic and neurogenic. Rectilinear biphasic waveform, low frequency and short pulse width stimulation was recommended.

The freshmen column in 39 985 Projectacademic libraries was investigated , which showed that 15 out of the 39 985 Projectacademic libraries have established freshmen column on their library website .Its posi-tion, contents and forms were analyzed.The typical freshmen column in libraries of Qinghua University, Shanghai Jiaotong University , and Sun Yat-sen University was described with suggestions put forward for improvement of its position, contents and forms.

Objective To investigate induction of the expression of human ?-defensin-2 (hBD-2) mRNA and the changes in activation of I-?B? and NF-?B by IL-1? in the human airway epithelium. Methods Primary human respiratory tract epithelium were stimulated with IL-1? and and/or PDTC. The expression of hBD-2 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). The I-?B? protein level in the cytoplasm was determined by Western blot, and the nuclear factor-kappaB (NF-?B) binding activity was analyzed by electrophoretic mobility shift assays. Results The hBD-2 mRNA expression could be detected after 1.5 hours of IL-1? stimulation at concentration of 1?g/L, and the expression of HBD-2 was in a dose dependent manner. Stimulation with IL-1? resulted in a reduction of I-?B? protein levels. The supershifts assays indicated that the p65-p50 heterodimer complexes of NF-?B were involved in the activation of NF-?B. Conclusions I-?B? can induce the expression of HBD-2 mRNA in a dose dependent manner; the p65-p50 heterodimer complexes of NF-?B play an important role in the regulation of hBD-2 gene expression in response to IL-1?.

Objective To analyse CT appearances of fibroma and fibrosarcoma and to provide evidences for clinical diagnosis and treatment.Methods 8 cases with surgically-and pathologically-proved hard fibroma(nasal cavity,maxillary sinus and soft palate each and all 2 cases)and fibrosarcoma(nasal cavity and maxillary sinus each and all 1 case)were studied with retrospectively analysis.The scanning parameters were 5 mm thickness and interval for axial scanning.Results CT appearances of fibroma were single circular,semicircular or elliptical soft tissue masses which usually had homogeneous density,clear margin,thin and full envelope,wide base or a pedicel and slight or middle enhancement after contrast.The tumors grew distensibly,bones near the mass were constricted and thinned or absorbed but never destroried and the structures nearby were deviated but not involved.Fibrosarcoma showed such common CT manifestations as other malignant tumors in the same locations.Conclusion CT appearances of fibroma has some features,they are different from more common benign lesions.Fibrosarcoma can not be differeciated from other maglinant tumors,thier diagnosis must depend on pathology.

Objective To evaluate the therapeutic effect of implantation of artificial urinary sphincter (AUS) for treatment of patients with trauma-induced genuine stress incontinence. Methods Two patients (1 male and 1 female) with genuine stress incontinence due to urethral rupture underwent implantation of AUS device (AMS 800).In the male case,the cuff of the AUS device was used to wrap the bulbar urethra.The reservoir was placed into the retropubic space.The controllable pump was implanted into the right scrotum. In the female case,the cuff was used to wrap the neck of bladder.The reservoir was placed into the retropubic space. The pump was implanted into the right subcutaneous labium majus.The literature was reviewed to show the indication,efficacy,complication and durability of implantation of AUS device. Results After operation the male patient was followed up for 17 months,and he achieved complete continence.The female was followed up for 14 months,and she had significant improvement in continence with changing 1 or 2 small pads every day.There were no infection, erosion, urethral atrophy and mechanical failure during follow-up.The review of the literature showed that in patients with implantation of AUS the mean of continence rate was 85.5%;the incidence rates of infection,erosion,urethral atrophy and mechanical failure were 6.1%,7.5%,6.1% and 16.7%,respectively. Conclusions The implantation of AUS is an effective,reliable method for patients with trauma-induced genuine stress incontinence.

Objective To explore a simple and effective method of culturing primary human bronchial epithelial cells.Methods Bronchial epithelial cells were obtained from human bronchial tissues obtained from lobectomy or pneumectomy.Epithelial cells were isolated by three methods as fellows:1.proteinase XIV digestion with mechanical scraping,2.proteinase XIV digestion with mechanical stripping,and 3.trypsin digestion with mechanical scraping.Subsequently the cells were cultured in serum-free hormone-supplemented DMEM/F12.The quantity,purity and viability of cells isolated by three methods were compared.The cultured cells were identified with cytokeratins AE1/AE3 by immunohistochemistry.Results The quantity of cells [(9.37?1.62)?105] obtained from proteinase XIV digestion with mechanical scraping was significantly higher than that of proteinase XIV digestion with mechanical stripping [(5.20?0.75)?105](P

Objective To discuss the application effect of fansclub informatization on the self-management of breast cancer patients. Methods A total of 240 breast cancer patients were equally divided into the experimental group and the control group by the random number table with 120 patients in each group. The control group was provided with routine health education, based on this, patients of the experimental group participated in the fans club. The compliance, tempering effect, readmission rate and the satisfaction degree were evaluated between the two groups one year later. Results A significant difference was observed between the two groups′ compliance (t=11.08, P<0.01), with the experimental group graded 69.27 ± 8.02 and the control group graded 48.76 ± 7.51. Moreover, in the experimental group, the angel of abduction, anteflexion, rear protraction, medial rotation, lateral rotation were respectively (170.13 ± 3.16)° , (172.45 ± 1.94)° , (46.71 ± 1.86)° , (81.30 ± 2.47)° and (85.18 ± 1.55)° ,which were significantly higher than the control group (125.16±1.93)°, (136.28±4.67)°, (34.63±2.68)°,(61.59±1.71)° and(85.18 ± 1.55)° (t=22.74-73.76,P<0.01). Further more, there were also remarkable differences (χ2=32.97, 54.21, 15.34, P<0.05) between the two groups on the consultative, readmission and the satisfaction degree, which in the experimental group were 91.67% (110/120), 81.67% (98/120), 96.67%(116/120), and in the control group were 55.83% (67/120), 47.50% (57/120), 84.17% (101/120). Conclusions The establishment of the fans club informatization remarkably improves the self-management of the breast cancer patients, which are worth popularizing.

Objective To investigate the safety and efficacy of surgery in 121 patients with spinal metastases.Methotds A retrospective analysis of clinical data from April 2009 to March 2013 was performed in 121 patients with spinal metastases.From 37 to 65 years,69 males and 42 females with mean age of 55.6 years.Primary tumor origin:Lung 35(28.9％),Breast 26(21.4％),Renal 17 (14.0％),Prostate 20 (16.5％),Thyroid 14 (11.6％),Liver 2 (1.7％),Colon 1 (0.8％),other 6 (5.0％).All patients received surgery.Follow-up and survival time were analyzed.In preoperation and postoperative 3 month,pain levels were assessed by visual analogue scale (VAS),neurologic deficit was evaluated by Frankel Grade and functional impairment was classified by Karnofsky Score.The quality of the life was assessed by EORTC QLQ-C30 questionnaire.Survival analysis was evaluated by Kaplan-meier.Results The period of follow-up ranged from 5 to 35 months with the average of 15.9 months.The mean survival was 14.5 months.1-year survival was 53.5％.2-year survival was 36.5％.In patients with lung cancer,the mean survival was 8.5months.1-year survival was 14.3％.2-year survival was 11.4％.In patients with breast cancer,the mean survival was 31 months.1-year survival was 57.7％.2-year survival was 46.2％.In preoperation and postoperative 3 month,the VAS showed statistical significance (t=21.6,P＜0.01) ;Post-operatively,80.3％ of all patients had functionally useful Frankel Grade D or E compared with 43.5％ pre-operatively.KPS score (80-100) percentage was 75.6％ postoperatively compared with 33.4％ preoperatively.In 1month postoperatively,35 of 75 patients who were sphincteric dysfunction preoperatively were improved.The EORTC QLQ-C30score was 83.39±7.23 in preoperation and 51.34±14.27 in postoperaion.The quality of life was impoved significantly (t=12.6,P＜0.01).Conclusion Surgical treatment was effective in improving quality of life by providing better pain control,enabling patients to regain or maintain mobility,and offering improved sphincter control.In all patients,the number of patents with spinal metastases from breast and lung cancer is higher.Compared with spinal metastases from breast cancer,the proportion of lung cancer origin received surgery is higher.

Objective To investigate the clinical efficacy of acupuncture at point Shanzhong(CV17) plus cupping on Back-Shu points in treating migraine. Methods Seventy migraine patients were randomly allocated to treatment and control groups, 35 cases each. The control group received conventional acupuncture and the treatment group, acupuncture at point Shanzhong(CV17) plus cupping on Back-Shu points in addition. The VAS score was counted in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups. Results The cure and marked efficacy rate and the total efficacy rate were 88.6%and 100.0%, respectively, in the treatment group and 62.9%and 94.3%, respectively, in the control group. There was a statistically significant difference in the cure and marked efficacy rate between the two groups (P＜0.05). There was a statistically significant pre-/post-treatment difference in the VAS score in the two groups (P＜0.05). There was a statistically significant post-treatment difference in the VAS score between the treatment and control groups (P>0.01). The clinical symptom improvement rate was (78.3±10.6)%in the treatment group and (49.8±11.2)%in the control group;there was a statistically significant difference between the two groups (P＜0.01). Conclusion Acupuncture at point Shanzhong plus cupping on Back-Shu(CV17) points is an effective way to treat migraine.

20%ALA cream were applied topically to condylomata acuminata.The cream was kept in place for 4 h.He-Ne laser light at 630nm was used,and the dose of light was 100 J/cm2 for all of the patients.After three treatment,the complete removal rate(CRR)of urethral and other genital mucossa were 93.4%and 88.9%,significantly higher than genital skin 39.1%(P＜0.05).The adverse reactions of ALA-PDT are mainly local minor erosion,short-term pain,but no scar.It showed that ALA-PDT is an effective,minimally invasive treatment for condylomata acuminata,especially for the lesions on urethral and genital mucosa.