Objective:To investigate the clinical and pathological factors of metastasis in patients with oral squamous cell carcinoma (OSCC)and clinical lymph node-negative(cN0).Methods:85 patients of OSCC with cN0 treated in 2008-07 -2013-07 were inves-tigated retrospectively.Results:In the 85 patients 24(28.34%)showed occult metastasis.Patient's gender,lesion location had no im-pact to metastasis(P >0.05),the patient's age,tumor size,growth pattern and histopathologic grade showed significant influence on the metastasis of OSCC(P <0.05).Conclusion:The greater tumor volume,lower differentiation degree and young age are the factors of higher occult lymph node metastasis.Selective neck dissection treatments of OSCC is recommended.

Retinoblastoma is the most common intraoeular malignant tumor in children.In its treatment,drug therapy is one of the most effective and irreplaceable methods.To reduce the toxicity of drugs,enhance the therapeutic effect and lessen drug resistance, some new drug deliver systems and new medicines for retinoblastoma are coming into being.Ophthalmic arterial infusion,fibrin sealant and iontophoresis are newly-found drug deliver systems.And the latest drugs under research include nutlins,phenoxazine derivative Phx-1,combretastatin A4 phosphate,2-deoxy-d-glucose and histone deacetylase inhibitors,etc.The following text is focused on the two aspects of the drug therapy for retinoblastoma.

Objective To evaluate the value of X-ray imaging in the diagnosis of multiple myeloma.Methods The findings of X-ray plain films of 136 patients with multiple myeloma were retrospectively analysed.Results Abnormal radiological changes were found in 117 of 136 patients (86.03%),of which 84 cases(61.76%) were located in skull,61 cases(44.85%) in spinal column,47(34.56%)in pelvis,51(37.5%)in humerus,41(30.15%)in ribs,29 (21.32%)in femur.In addition,clavical was involved in 15 cases,scapula in 13,radius in 9,tibia in 9,fibula in 4 and ulna in 3.Conclusion X-ray plain film is of important effect on the diagnosis of multiple myeloma.

Bone marrow mesenchymal stem cells(BMSCs) have the ability of multi-directional differentiation.In different environments,it can be induced into various blastodermic layer cells,such as osteoblast,chondrocyte and so on.Intervertebral disc degeneration(IDD) is mainly the change of nucleus pulposus cells and extracellular matrix.Nucleus pulposus cell is a chondrocyte-like cell.With the progress of cell bioengineering and molecular biology,the tissue engineering nucleus pulposus cells can be regenerated by BMSCs combined with cell carrier.It can be implanted into intervertebral disc to prevent and reverse IDD.It will be a new way for treating IDD.

Objective To investigate the changes of myoepithelial cells in mammary atypical hyperplasia and breast cancer. Methods SP immunohistochemistry was used to detect actin expression in normal breast tissue, grade Ⅰ, Ⅱ and Ⅲ atypical hyperplasia and breast cancer. Electromicroscopy was used to observe the changes of ultrastructure of myoepithelial cells. Results Actin was only detected in myoepithelial cells of normal breast tissue and grade Ⅰand Ⅱ atypical hyperplasia. The positive expression rates of actin in grade Ⅲ atypical hyperplasia(70%) and breast cancer(90%) were significantly higher than that in grade Ⅱ atypical hyperplasia(10%), P

Fracture healing is regulated by a complex array of factors,of which calcitonin gene-related peptide(CGRP) has been shown to play an anabolic role in bone metabolism.It has been reported that CGRP-containing sensory nerve fibers increased apparently at bone fracture site.CGRP is an important sensory neuropeptides expressed in bone tissue,although the primary function of this neuropeptides is to induce vasodilatation and blood flow,substantial evidence has shown that targeted expression of CGRP to mouse osteoblasts increased bone density,stimulated cancellous bone formation in vivo,especially the presence of CGRP receptors on rat osteoblast provides further evidence for a direct role for CGRP in modulating the function and growth of bone.CGRP is also proved to be a neuropeptide with proinflammatory activities,it may play a direct role in initiating the local inflammatory cell infiltration in the fracture site.Although it has been recognized that CGRP plays an important role in bone repair and reconstruction,the mechanism of this activation still remains to be elucidated.

Ferroptosis is a newly discovered regulatory mode of cell death, which is caused by glutathione peroxidase 4 deficiency, abnormal iron metabolism and lipid peroxidation.At present, it is considered that iron metabolism and active oxygen metabolism are the central link of ferroptosis.Ferroptosis involves a variety of physiological and pathological processes, including cancer cell death, neurotoxicity, ischemia-reperfusion injury, and T-cell immunity.Studies have shown that ferroptosis characteristics such as iron overload and lipid peroxidation may occur in different degrees during the development of a variety of nephropathy.Ferroptosis can affect the progression of renal disease by regulating the level of intracellular iron ions and lipid peroxidation.Therefore, effective regulation of ferroptosis is expected to be an important strategy in the treatment of renal diseases.In this paper, the regulation mechanism of ferroptosis and its research progress in kidney disease are reviewed to provide new theories and ideas for the treatment of renal disease.

Objective:To investigate the expression of pre-B-cell leukemia homeobox 3 (PBX3) and the phosphatase and tensin homology deleted on chromosome 10 (PTEN) in cervical cancer and its relationship with the clinicopathologic characteristics and prognosis.Methods:Cervical cancer tissues and adjacent tissues of 85 patients with cervical cancer admitted to Xianlin Gulou Hospital from June 2014 to December 2018 were collected and the expression levels of PBX3 and PTEN were detected by immunohistochemistry. The univariate analysis and Logistic regression model were used to analyze the relationship between the expression levels of PBX3, PTEN and clinicopathologic features. Kaplan-Meier survival curve was used to analyze the relationship between the expression levels of PBX3, PTEN and prognosis.Results:The positive expression rate of PBX3 protein in cervical cancer tissues was higher than that in adjacent tissues: 38.82%(33/85) vs. 25.53%(20/85); the positive expression rate of PTEN protein was lower than that in adjacent tissues: 36.47%(31/85) vs. 98.82%(84/85), and there were significant differences ( P<0.05). The univariate analysis showed that the expression levels of PBX3 and PTEN were associated with clinical stages, degree of tumor differentiation, lymph node metastasis, vascular invasion, and degree of tumor invasion ( P<0.05). The multiple Logistic regression model showed that the clinical stages, tumor differentiation and lymph node metastasis were independent influencing factors for the positive expression of PBX3 or PTEN in cervical cancer tissues ( P<0.05). While 45.45%(15/33) of patients with positive PBX3 expression died, with a median survival of 31 months, and 25.00% (13/52)of patients with negative expression died, with a median survival of 38 months. Kaplan-rank test showed that the survival time in the patients with positive PBX3 expression and in the patients with negative expression had significant difference ( P=0.025). While 22.58%(7/31) of patients with positive PTEN expression died, with a median survival of 39 months, and 38.89%(21/54) of the patients with negative expression died, with a median survival time of 33 months. Kaplan-rank test showed that the survival time in the patients with positive PTEN expression and in the patients with negative expression had significant difference ( P=0.035). Conclusions:The expression of PBX3 is up-regulated and PTEN is down-regulated in cervical cancer. The expression levels of PBX3 and PTEN are related to clinical stage, tumor differentiation and lymph node metastasis. The prognosis of the patients with positive PBX3 expression is worse than that of the patients with negative expression, and the prognosis of the patients with positive PTEN expression is better than that of the patients with negative expression.