From January, 1960 to April. 1983, 292 cases of primary liver cancer were treated by liver resection. Of them, 21 cases underwent re-exploratory operation for relapsed reason and 14 tumours were removed with a resectability rate of 66.7%.In this series recurred tumours in 3 patients were resected by a two-stage operation with hepatic artery ligation or embolizaton as the first step.Following-up results: 7 out of 14 resected cases survivors, 2 survived above one year, 4 more than half a year and 1 less than half a year; in another 7 cases died post-reoperativelly, 1 above one year, 4 more than half a year, 1 less than half a year.As for AFP detection 4 patients were negative and in 1 patient the AFP titre decreased to 100 ug/ml on the 33rd post-reoperative day. From the above data the authors believe that re-operation may be considered as a most effective method if it is performed at a proper time and radiotherapy, hepatic artery ligation or embolization are of great value in treating recurred cases. For the improvment of the surgical results of primary liver cancer,a preliminary scheme for investion and management procedure for such patients is suggested by the authors as well.

Objective To analyze the enzyme kinetics of active ingredient of Buddleja lindleyana(AIBL)against Oncomela?nia hupensis,the intermediate host of Schistosoma japonicum. Methods O. hupensis snails were placed in 1 000 ml of 3.55 mg/L AIBL solution for 24,48 h and 72 h,respectively,and the enzyme kinetics of alanine aminotransferase(GPT)was deter?mined by Reitman?Frankel assay,lactate dehydrogenase(LDH)by the chemical inhibition lactic acid substrate method,alka?line phosphatase(AKP)by the disodium phenyl phosphate colorimetric method,acetylcholine esterase(AChE)and malate de?hydrogenas(MDH)by ELISA,and succinate dehydrogenase(SDH)by the phenazine methyl sulfate reaction method(PMS)in the soft tissues of O. hupensis before and after AIBL treatment. Results Following exposure to 3.55 mg/L AIBL solution for 24 h, the GPT,LDH,and AKP activities significantly improved in the soft tissues of O. hupensis,while the SDH and MDH activities were significantly lowered in the head?foot and liver. However,AIBL treatment did not cause significant effect on AChE activity in O. hupensis. Conclusions AIBL causes significant damages to O. hupensis liver and can efficiently act on anaerobic and aer?obic respiration loci,which will hinder energy metabolism,and cause inadequate energy supply in cells used for normal secre?tion,eventually leading to O. hupensis death.

Objective To establish the plane strain model of brain tissues on mid-sagittal plane and discuss the relation between the brain tissue deformation and brain injury. Methods By referring to the computerized tomography pictures of the human body and using plane strain and finite element method (FEM), we applied plane strain hypothesis to establish a model for calculation of the brain tissue deformation caused by rotational inertia loading during vehicle crash accident. Results At the 12th ms, the shear strain field adjacent to parietal lobe reached 0.148 and the maximum positive shear stress located at white material of the cerebellum was up to 8 088.0 Pa. At the 28th ms, the maximum shear stress located at the cerebellum reached 900.9 Pa. Great shear strain existed in the cerebrospinal fluid region all the time and shear stress with large absolute value presented on the boundary of white matter and gray matter. Conclusions Under rotational inertia loading, the shear strain (stress) in brain tissues can lead to diffuse axonal injury. The uneven strain (stress) may cause serious avulsion damage in brain tissues.

Objective To understand the prevalence of major non-communicable chronic diseases among the adults in the airport district of Beijing.Methods With the same proportion and multi-stage stratified cluster sampling,the prevalent rates of primary existing chronic diseases were investigated in 650 residents aged over 18 years and lived in Beijing for more than 6 months.Each participant was invited to receive a set of standardized questionnaire,physical examinations and laboratory tests.Results The prevalent rates of hypertension,diabetes,dyslipidemia,acute myocardial infarction and the stroke were 26.0%,5.3%,39.9%,1.3% and 2.0%,respectively.The awareness rates for hypertension and diabetes were 54.4% and 3.6%,respectively.Conclusion The prevalent rates of chronic non-communicable diseases of the adults in this area were in higher levels.The relevant department should pay more attention to it.

Objective To observe the effects of paclitaxel,mitomycin,5-FU and cisplatin on the proliferation of tongue carcinoma cells and to guide the reasonable using of chemotherapeutic drugs and avoids the side-effects.Methods The fresh tongue carcinoma tissues from surgery were collected and unicellular suspension were prepared.Then paclitaxel,mitomycin,5-FU and cisplatin were used to investigate the drug sensitivity on primary tongue carcinoma cells and a blank control group was set up.The inhibitory effects of chemotherapeutic drugs on the growth of tongue carcinoma cells and the cell cycle phases were observed by MTT colorimetry and flow cytometry.Results The inhibitory rates of paclitaxel,mitomycin and 5-FU on tongue carcinoma cell proliferation were 45.3%,37.3%,and 36.2%,respectively,and in control group it was 2.1%,the difference was significant(P

ObjectiveTo observe the effect of Chinese medicinal application at Yongquan (KI1) on hot flush and sweating brought by using GnRH-ain patients with endometriosis.MethodTotally 120 patients diagnosed with endometriosis of stageⅢ~Ⅳ were randomized into a treatment group and a control group. The control group started to receive GnRH-a(Goserelinum) at 3.6 mg/28 d since post-operational day 3, 4 times in total; the treatment group started to receive GnRH-a(Goserelinum) at 3.6 mg/28 d since post-operational day 3, 4 times in total; at the night of taking GnRH-a, the treatment group was also given Chinese medicinal application at Yongquan, a month as a treatment course, successively for 4 courses.ResultThe side effects of using GnRH-a, including hot flush and sweating, were significantly less in the treatment group than that in the control group (P<0.05).Conclusion Medicinal application at Yongquan can significantly reduce the side effects including hot flush and sweating brought by using GnRH-ain patients with endometriosis.

Objective To study the clinical features and differential diagnosis of hairy-cell leukemia variant (HCL-V).Methods A case with HCL-V was reported and the literatures were reviewed.Results The patient had splenomegaly for twenty years and a history of recurrent pulmonary infection.His blood routine test showed a high white blood cell count and abnormal high proportion of lymphocytes.Peripheral smear and bone marrow smear both showed significantly higher proportion of lymphocytes,part of which had soma jagged,prominent nucleoli and villous/hairy cytoplasmic projections.His hairy leukemic cells expressed CD1 1c,CD19,CD20,CD22,and had variable expression of FMC-7,CD103 and lambda,but not CD5,CD23 and CD25.Transmission electron microscope showed many monocytes with villous exist in peripheral blood.Conclusions HCL-V is a rare and an indolent form of a small,mature,B-cell leukemia,based on the clinical,peripheral smear,bone marrow smear,flow cytometric analysis and transmission electron microscopy features,a diagnosis of HCL-V is confirmed.The differential diagnosis should always include splenic marginal zone B-cell lymphoma and HCL-C,because they have different clinical and biological features.

0.05). The LDH was lower ,and cell viability was higher in group IPC than those in control group. The LDH was higher ,and cell viability was lower in grou p SMT, group NS 398 and group SB than those in group IPC. CONCLUSIONS: In late p hase of preconditioning ,the p38MAPK activity and expression of iNOS and COX 2 increase significantly in rat cardiomyocytes, activited p38MAPK mediates iNOS ,then promotes COX 2 expression.

Objective:To investigate the role of the transient receptor potential melastatin subfamily member 7 (TRPM7) ion channel in the proliferation and migration of oral squamous cancer cells OC2.Methods:The expression of TRPM7 in OC2 cells was examined by western blotting,RT-PCR and indirect immunofluorescence assay.The influence of TRPM7 on cell proliferation and migration under the inhibition of TRPM7 ion channel with 2-APB or siRNA TRPM7 in OC2 cells was examined by MTT and Transwell respectively.The influence of PI3K/AKT signal pathway on the expression of TRPM7 in OC2 cells was tested by Western blotting.The change of positive ion current when the OC2 cells were treated with TRPM7 inhibitor and activator was explored with patch clamp technique.Results:TRPM7 overexpression in OC2 cells mainly in the cytoplasm was observed,siRNA TRPM7 downregulated the expression of TRPM7.Inhibition of TRPM7 (2-APB or siRNA TRPM7) inhibited the growth and proliferation of OC2 cells in a time-and dose-dependent manner.Blockage of TRPM7 ion channel inhibited the migration capacity of OC2 cells.Inhibition of TRPM7 downregulated the expression of phosphorylated AKT and phosphorylated ERK.Patch clamp assay showed the activation of TRPM7-like current in OC2 cells.2-APB could weaken this current while Bradykinin could enhance the current.Conclusion:TRPM7 may regulate the proliferation and migration of oral cancer cells by the regulation of PI3K/AKT and MAPK/ERK signal pathways and cell positive current.

The clinical application of delayed double knotting over the entire microvascular ananstomotic for free tissues transform has the advantages of direct vision and it easy approachs,is useful to avoid the opposite wall biting and other mistakes.It is especially feasible in the management of the cases with endangium denudation.