Objective To study the effect of Abnormal Savda Munziq (ASMq) on MDR and expression of mRNA of Bel/Fu cell line. Methods Several genes expression including MDR1, MRP, TOPOII? and GST-? were analyzed by RT-PCR. Results ASMq has been found to down-regulate MDR1 gene and simultaneously up-regulate TopoⅡ?mRNA. Conclusion ASMq can reverse the drug resistance against 5-Fu in Bel/Fu cells. Inhibition of MDR1 mRNA and up-regulate TopoⅡ? mRNA may contribute to the mechanisms.

OBJECTIVE: To investigate the protective effect of abnormal savda munzip on the radiation-induced damaged in mice. METHODS: The mice were randomly divided into 2 groups: a control group and a drug group; the latter was again divided into a low dosage group (6.25%), a moderate dosage group (12.5%) and a high dosage group (25%) according to the drug concentration. All mice were given general radiation emitted by 6 Gy MV-X liner accelerator once, which resulted in the acute radiation damage. The different administrations of abnormal savda munzip were fed to the mice in the drug group orally for 7 days consecutively before and after the radiation. On the day of the last administration, 10 mice were sacrificed, and white blood cells, red cells and blood plates of the peripheral blood, spleen index, and spleen SOD were detected; while the 20 remaining mice were fed continually till 30 days, and the 30 day survival ratio of the mice was calculate. RESULTS: (1) Although the mice weight in the drug groups was higher than that in the control group, no significant difference existed (P>0.05). Spleen index in the control group was higher than that in the drug group. (2) Spleen SOD was significantly increased by the drugs compared with that of the control group (P<0.01), which was positively related to the drug concentration. (3) Pre-treatment of mice with the extract of abnormal savda munzip significantly increased the white cells and red cells in the peripheral blood compared with the control group (P<0.05). The blood cells were positively related to the drug concentration (P<0.05). Although the counts of blood plates in all drug groups were higher than those in the control group, no significant difference existed (P>0.05). (4) Compared with the control group, a marked improvement of 30 day survival rate in all drug groups was observed (P<0.0001). Both the concentration and the survival rate were increased (P<0.01). (5) The SOD level and leukocyte counts in the irradiated mice were positively related to the survival ratio (r=0.612, P<0.005; r=0.32, P<0.05, respectively). CONCLUSION The abnormal savda munzip has significant protective function on the mice irradiated by 7 Gy MV-X liner accelerator.
Administration, Oral ; Animals ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Male ; Mice ; Radiation Injuries, Experimental ; drug therapy ; mortality ; Radiation-Protective Agents ; administration & dosage ; therapeutic use ; Random Allocation ; Spleen ; drug effects ; enzymology ; Superoxide Dismutase ; metabolism ; Survival Rate ; Time Factors ; Whole-Body Irradiation