Bronchopulmonary dysplasia is one of the causes of affecting children′s health,which has a complicated etiology,including the gene level of internal aspect and some external factors, such as premature birth inducing the pulmonary hyperplasia,infectious diseases caused by low immunity,complications caused by clinical interventions,and the low quality of respiratory tract management. In the article,we review the research progress about mechanism and prevention of BPD in domestic and overseas in recent years.

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Medical technology center can promot the development of the desciplines concerned.This paper discusses the feasibility and effect of multidisciplinary construction of medical technology center.We conclude that multidisciplinary participation is beneficial for the center,as these disciplines have respective strengths and are mutually complementary.

Objective:To study the natural progress of chronic periodontitis and its association with IL-1 genetic polymorphisms.Methods:166 subjects with chronic periodontitis were examined at baseline and in 1 year after 1st visit by measuring the probing depth(PD),attachment loss(AL) and bleeding on probing on 6 sites of each tooth.DNA samples were obtained with buccal swabbing technique and genotyped for IL-1-889 and IL-1+3954 genetic polymorphisms with PCR-RFLP in 111 cases of the subjects.Results:The mean PD,AL increace was 1.12 mm and 1.628 mm respectively within 1 year.249 teeth had more than 2 mm attachment loss over one year period in 94 patients.There was no significant difference for the distribution and frequency of IL-1A-889 genotypes and alleles between the rapidly progressive group(with AL≥2 mm at least one site) and the non-rapidly progressive group.The distribution of IL-1B+3954 genotype 1/2 and the frequency of the allele 2 were significantly higher in the rapidly progressive group than that in the non-rapidly progressive group(P

Objective To investigate the curative effects of laparocopic treatment for cystiduodenal internal fistula. Methods All procedures were conducted under laparoscope. After the exposure of the cholecystic base, trans-cholecystic cholangiography was performed to detect the relations between the cholecyst and neighboring organs. Once the internal fistula was confirmed, the orifice of fistula was closed laparoscopically. Then abdominal drainage, gastrointestinal decompression, and administration of somatostatin were routinely employed. Results In a series of 327 cases of laparoscopic cholecystectomy (LC) with intraoperative cholangiography, a total of 6 cases of cystiduodenal internal fistula were detected, all of which were anatomized and closed successfully under laparoscope. No postoperative hemorrhage or duodenal bile leakage occurred. Conclusions Routine intraoperative cholangiography during LC is beneficial to the detection of the cystiduodenal internal fistula.Effective and reliable management rests upon skillful laparoscopic techniques of dissection and fistula closure, as well as the supportive treatment.

Objective To explore the effect of FESS on nasal resonance characteristics in patient with chronic sinusitis .Methods Detected 50 cases of adult patients with type Ⅱ chronic sinusitis ,and collected the date about the application of multi-channel voice analysis system that tested the pronunciation /i/before and three months af-ter FESS spectrum - energy distribution while compared with 50 normal subjects .Results Patients with type Ⅱchronic sinusitis before surgery compared with normal subjects ,the head of the low -frequency energy was higher , high-frequency was lower ,the difference was statistically significant (P<0 .001) .Voice energy distribution after three months compared with preoperative ,the head of the high-frequency resonance energy improved than preop-erative ,but still relatively low compared with the normal ,the difference was statistically significant (P<0 .05) . While the mouth ,chest and throat in addition to oral FR3 ,FR4 ,postoperative throat FR4 and preoperative chest FR4 ,other frequency -energy compared with normal subjects no significant change was not statistically significant (P>0 .05) .Conclusion For patients with chronic sinusitis ,low -frequency of the voice of nasal resonance de-creased ,and high-frequency increased after FESS .

Objective: To examine the distribution of IL-1B+3953 genotypes of Han nationality people with different periodontal health status and to evaluate if there is an association between the genotype of IL-1B+ 3953 and the severity of periodontitis. Methods: 271 subjects of Han nationality were selected, among them there were 85 cases with severe chronic perioodontitis(group①), 97 cases with light and mild chronic periodontitis (group ②) and 89 subjects of periodontal healthy control(group ③). Full periodontal examinations were taken including clinical periodontal tissue attachment loss measurements of full-mouth, periodontal pocket depths and bleeding on probing. DNA samples were obtained with buccal swabbing technique and were further analyzed for IL-1B+3953 genotype polymorphisms using PCR-RFLP-based method. Results: In group ① the distribution (%) of Ⅰ / Ⅰ, Ⅱ / Ⅰ and Ⅱ / Ⅱ was 80, 20 and 0; In group ② 85. 57, 14. 43 and 0; in group ③ 95. 51, 4. 49 and 0, respectively(p

Objective To explore the role and mechanism of keratin 19 (KRT19) in breast cancer.Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to determine KRT19 levles in 35 cases of breast cancer tissues and normal tissues.The correlation between KRT19 levels and clinical property of breast cancer was analyzed.Meanwhile,the expression levels of KRT19 in several breast cancer cells and mammary epithelial cell Michigan cancer foundation (MCF)-10A were evaluated by qRT-PCR assay.Over-expressed and knocked-down KRT19 in breast cancer ceils,3-(4,5-dimenthylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed to detect the proliferation and chemosensitivity of these cells.The ability of forming colon of these breast cancer cells with treated KRT19 was studied via colony-forming unit assays.Western blot assay was performed to determine expression levels of ceil cycler related proteins.Results KRT19 was upregulated in breast cancer tissues comparing to normal tissues.KRT19 was positively related to tumor node metastasis (TNM) stage and distant metastasis of breast cancer.Similarly,KRT19 was highly expressed in breast cancer cells compared to mammary epithelial cell MCF-10A.The proliferation and colony-forming ability was significantly enhanced in MCF-7 cell with o,verexpressed KRT19.MTS assay showed that chemosensitivity of MCF-7 cells with overexpression of KRT19 was much more remarkably reduced than the control group.However,knocking down KRT19 in breast cancer cells MDA-MB-231 got the opposite results.Western blot assay suggested that KRT19 could obviously upregulated cyclin-dependent kinase 1 (CDK1) but not p27 expression levels.Conclusions KRT19 was upregulated in breast cancer tissues and was positively related to TNM stage and distant metastasis of breast cancer.KRT19 can significantly enhance proliferation and chemoresistance of breast cancer cells via upregulating CDK1.

Objective To establish the method for determination of astragaloside Ⅳ in Fufang Buqi Yangxue Oral Solution by RP-HPLC.Methods The content of astragaloside Ⅳ was determined by a HPLC system under the following conditions:the chromatographic column was Agilent C18(150 mm? 4.60 mm,5? m),the mobile phase was acetonitrile-water(60:40),the flow rate was 1.0 mL? min-1,and the detection wavelength was at 203 nm.Results Astragaloside Ⅳ showed good linearity in the range of 0.34～ 3.40 ?g(r=0.999 9).The average recovery was 100.8 %,and RSD was 1.47 %(n=6).Conclusion The HPLC method is simple and reproducible,and can be used for quality control of Fufang Buqi Yangxue Oral Solution.

SUMMARY Intravesical bacillus Calmette-Guérin ( BCG) was a common treatment for non-muscle inva-sive urothelial carcinoma of the bladder. The complication of prostatic abscess was rare. We reported a case of tuberculous prostatic abscess after BCG therapy. A 65-year-old man was diagnosed as bladder cancer and accepted transurethral resection of bladder tumor ( TURBT) treatment. He received a 6-week induction course without any infection complication. Following the second BCG maintenance instillation, he complained of fever and dysuria. Transrectal ultrasound ( TRUS) demonstrated a well-defined complex mass in the right lobe of his prostate. The diagnosis of tuberculous prostatic abscess was considered after excluding other bacterial infection. The patient was treated with an anti-tuberculous regimen of isoniazid, rifampicin, and ethambutol. The symptoms were relieved after 4 weeks of anti-tuberculous therapy. Be-cause of the good response to the medicine, no further aspiration or drainage of prostatic abscess was car-ried out. The anti-tuberculous therapy had to be stopped for serious drug induced liver injury after 6 weeks of anti-tuberculous therapy. Eight weeks later of stopping anti-tuberculous therapy, the follow-up TRUS showed the disappearance of the prostatic abscess and the test of his liver function was normal. Considering the virulence of BCG is weaker than that of common tuberclebacillus, the shorter course of anti-tuberculous therapy maybe an alternative choice, and surgical drainage is not always necessary.