Objective To optimize the processing technology of Fructus Ligustri Lucidi.Methods A RP-HPLC method was carried out to determine the contents of oleanolic acid and ursolic acid in the fruits of Ligustrum lucidum and an orthogonal design to optimize the processing conditions with the indexes of oleanolic acid content and ursolic acid content.Results Linear ranges of oleanolic acid and ursolic acid were in 0.019 78～0.197 8 mg/mL(r=0.999 8)and in 0.010 26-0.102 6 mg/mL(r=0.999 8)respectively,and average recoveries of oleanolic acid and ursolic acid were 99.53 %(RSD=2.08 %)and 99.88 %(RSD=1.76 %)respectively.Orthogonal design showed the optimum conditions were as follows:soaking for 8 hours with 2 gram wine(5 gram of Fructus Ligustri Lucidi),then steaming for 8 hours.Conclusion HPLC is an accurate and quick method to determinate the contents of oleanolic acid and ursolic acid,and can be used as a content determination method for Fructus Ligustri Lucidi.Soaking time,steaming time and wine content have no significant influences on the contents of oleanolic acid and ursolic acid.

Objective　To analysis the relationship between the multipathogens infection and unhealthy pregnancy.Methods　All patients was selectively divided into three groups:group A including 106 patients with unhealthy pregnancy,group B:55 gravidas with normal induced abortion,group C:55 normal parturient with term labor.The ten kinds of pathogens from abortion tissues and placentas were tested by PCR method respectively.Anti-pathogen IgM antibodies were detected by ELISA in the blood specimens.Results　The distribution status of the pathogens were as followed:Human parvovirus B19(B19V 23.6%)＞Cytomegalovirus(CMV,16.0%)＞Toxoplasma (TOX，15.1%)＞Rubella virus(RV，10.4%)＞Ureaplasma urealyticum(9.4%)＞Coxsackie(7.5%)＞Herpes simplex virus(HSV,6.6%)＞Adenovirus,Eprstan Barr virus(3.8%)＞Chlamydia trachomatic(CT 2.8%) in the group A.The positive rates of the pathogens infection in both group C and group B were lower.The positive rates of B19V,CMV,RV,HSV,TOX were significant difference(χ2 test,P＝0.000～P＝0.031) among the three groups,hovever others were not(χ2 test,P＝0.121～P＝0.724).Conclusions　The pathogen infection is an important factor to occur the unhealthy pregnancy,the major pathogens of unhealthy pregnancy are B19V，CMV，TOX，RV，HSV.These pathogens infection should be detected timely in gestational period.

Chlamydia trachomatic(CT 2 8%) in the group A.The positive rates of the pathogens infection in both group C and group B were lower.The positive rates of B 19 V,CMV,RV,HSV,TOX were significant difference(? 2 test,P=0 000～P=0 031) among the three groups,hovever others were not(? 2 test,P=0 121～P=0 724).Conclusions The pathogen infection is an important factor to occur the unhealthy pregnancy,the major pathogens of unhealthy pregnancy are B 19 V,CMV,TOX,RV,HSV.These pathogens infection should be detected timely in gestational period.

Objective To investigate the effect of Suanzaoren Decoction on hippocampus, cortex BDNF and TrKB gene expression in depression model rats. Methods Depression rat models were established by social-isolated raise and chronic stress stimulation. Suanzaoren decoction was administrated to the models. RT-PCR was adopted to detect the expression of mRNA BDNF and TrKB genes. Results The mRNA expression of BDNF and TrKB in cortex of Suanzaoren decoction high dose group、medium dose group and clomipramine group(0.213±0.094, 0.639±0.023, 1.032±0.015, 1.089±0.014, 1.580±0.012, 1.860±0.019)were all higher than the model group(0.032±0.008, 0.001±0.000), showing a significant difference among four groups (P<0.01), and the mRNA expression of BDNF and TrKB in hippocampus of Suanzaoren decoction high dose group, medium dose group and clomipramine group(0.213±0.094, 0.639±0.023, 1.032±0.015, 1.089±0.014, 1.580± 0.012, 1.860±0.019)were higher than the model group (0.021±0.015, 0.125±0.013), there was a significant difference between four groups(P<0.01). Compared with the model group, the mRNA expression of low-dose group of Suanzaoren decoction in both cortex and hippocampus of BDNF and TrKB was not significantly different to the model group(P>0.05). Conclusion Suanzaoren decoction can increase the expression of BDNF and TrKB gene, promote neuronal proliferation, and resist depression.

Objective To investigate the correlation of the initial arterial lactate (Lac) and 6-h lactate clearance rate (LCR) with prognosis in patients with acute paraquat poisoning (APP).Methods The included 132 APP patients (70 males and 62 females,age ranging 15-71,30 (18) [M (IQR)]treated at a single center between January 2009 and December 2013.Patients were divided into two groups:group A,survivors ＞ 28 days after admission (n =64); and group B,those died ≤ 28 days after admission (n =68).The prognostic values of arterial Lac and 6-h LCR during the acute stage of poisoning were evaluated.Results Paraquat dose ranged from 5-200 mL,20 mL (37.25 mL) [M (IQR)].The average time from poisoning to arrival at the emergency department was ranging 4-312 h,6 h (8.75h) [M (IQR)].Total mortality was 51.51％.There were no differences in age,gender,and length of time elapsed from poisoning to diagnosis between two groups.Survivors had a significantly lower dose of paraquat ingested compared with nonsurvivors (P ＜ 0.05).An ROC curve analysis determined that the dose had an area of 0.86 (95％ CI:0.80-0.92) and the volume cut-off point was 27.5 mL to predict the prognosis in patients with acute paraquat poisoning (75％ sensitivity,85.9％ specificity,Youden index 0.609).The initial arterial lactate level was higher in nonsurvivors than that in survivors.The ROC curve analysis indicated that the initial arterial lactate level had an area of 0.99 (95％ CI:0.99-1.00) and the concentration cut-off point was 5.050 mmoL/L to predicti prognosis in patients with acute paraquat poisoning (sensitivity 98.5％,specificity 100％,Youden index 0.985).The 6 h LCR was lower in nonsurvivors than that in survivors.ROC curve analysis showed that the 6-h LCR had an area of 0.99 (95 ％ CI:0.97-1.00) and the concentration cut-off point was 17.28％ to predict prognosis in APP patients (sensitivity 100％,specificity 97.1％,Youden index 0.971).Conclusions In the early stages of APP,initial arterial Lac and 6-h LCR are closely related to prognosis and may serve as prognostic factors.

AIM: To evaluate the effects of lactacystin (LAC) and ?-lactacystin (?-LAC), proteasome inhibitor, on the proliferation and activation of T lymphocytes. METHODS: Flow cytometry was used to analyse the proliferation and the expression of CD69, CD25 and CD3 in PHA activated T-lymphocytes. Furthermore, the expression of PA28 and IL-2 mRNA were assayed by competitive RT-PCR. RESULTS: (1) LAC and ?-LAC significantly decreased the incorporation in PHA activated T-lymphocytes. (2) Although LAC and ?-LAC did not affect the expression of CD69 at any time, they significantly inhibited the expression of CD25 (48 h, 72 h, P

Objective: To observe the immunohistochemical expression of interferon-? (IFN-?), matrix metalloproteinase -9(MMP-9) in brain and spinal cord tissue, and explore the action mechanism of Zuogui Pills and Yougui Pills on rats with experimental autoimmune encephalomyelitis(EAE). Methods: Lewis rats were immunized with the myelin basic protein (MBP).120 Rats were grouped randomly into nomal group, EAE group, prednisone group, Zuogui Pills group and Yougui Pills group after post immunization (PI). Rats in normal group and EAE group were administered sodium, each 3ml/d, rats in prednisone-group were administered suspension of prednisone after developed clinical signs, each 5mg/kg, rats in Zuogui Pills group were administered suspension of Zuogui Pills, each 2g/kg, and rats in Yougui Pills group were administered suspension of Yougui Pills each 3g/kg. On days 15 and 27 PI, rats were killed and the immunohistochemical staining was performed on the sections of brain and spinal cord. Results: On days 15 and 27 PI, the expression of IFN-? and MMP-9 in central nervous system of EAE group were higher than those in nomal group (P

Objective To evaluate the efficacy of point application combined with catgut implantation at acupoint for lung-distention. Methods A total of 122 patients with lung-distension were randomized into a treatment group (62 patients) and a control group (60 patients). The patients in the control group received conventional treatment, and those in the treatment group were additionally treated with point application combined with catgut implantation at acupoint on the basis of the control group for 4 weeks. At 6 months follow-up, pulmonary function including the forced expiratory volume in first second (FEV1) and the percentage of FEV1 to forced vital capacity (FEV1/FVC), the total effective rate and acute exacerbation rate were compared in both groups. Results The total effective rate in the treatment group was significantly higher than that in the control group (98.4%vs. 85.0%;χ2=5.592, P<0.05). There were no significant difference in the acute exacerbation rate between the treatment group and the control group (8.1%vs. 20.0%;χ2=3.358, P>0.05).The FEV1 (treatment group: 71.58% ± 2.23% vs. 59.98% ± 2.19%, t=29.223, P<0.01; control group: 66.99% ± 2.25% vs. 61.03% ± 2.50%, t=13.726, P<0.01) and the FEV1/FVC (treatment group:68.99%± 1.33%vs. 50.77%± 1.45%, t=72.914, P<0.01; control group: 62.67% ± 1.47% vs. 51.89% ± 1.88%, t=34.989, P<0.01) after the treatment were significantly increased compared with before the treatment in both groups and such improvement in the treatment group was greater in the treatment group than that in the control group (FEV1: t=13.726, P<0.01;FEV1/FVC:t=34.989, P<0.01). Conclusion Point application combined with catgut implantation at acupoint can improve lung function and clinical effects in patients with lung-distention.

Objective To study the changes in the level of serum IMA in patients with cardiac arrest followed by CPR,and explore the value of serum IMA in predicting prognosis of these patients after CPR.Methods (1) A total of 36 patients (25 men and 11 women) after CPR following cardiac arrest were recruited into experimental group (group C).Of them,15 cases (11 men and 4 women) and 21 cases (14men and 7 women) were assigned into resuscitation group (group R) and non resuscitation group (group NR),respectively,according to the presence or absence of ROSC.Meanwhile,another 60 healthy subjects (39 men and 21 women) matched for age and gender were recruited into healthy control group (group H).(2) The determination of the serum IMA level and comparisons of changes in serum IMA levels among groups were carried out.Results (1) The level of serum IMA in group C was higher than that in group H (P ＜ 0.05) ; The serum IMA level in group R was lower than that in group NR and higher than that in group H (P ＜0.05 for both).(2) The area under the ROC curve for serum IMA in predicting the prognosis of patients treated with CPR after cardiac arrest was 0.73.When the cut off value was 128.25 U/mL,the sensitivity and specificity were 57.1％ and 93.3％ respectively; the positive predictive value and negative predictive value were 92.3％ and 60.9％ ; positive likelihood ratio and negative likelihood ratio were 8.5 and 0.5 ; the correct diagnosis index was 72.2％,and Youden index was 50.5％.Conclusions Serum IMA has a certain value in prediction of prognosis in patients treated with CPR following cardiac arrest.

ObjectiveTo observe the effect of estrogenic-like effects ofWujia-Shenghua capsule and effective medication site.MethodsThrough D-101 macroporous resin column methods,Wujia-Shenghua capsule with 60% ethanol extraction was separated for water elution part, 20% ethanol elution part, 40% ethanol elution part, 60% ethanol elution part. Then each elution part was respectively mixed into concentration of 10mg/ml,1mg/ml, 0.1mg/ml, 0.01 mg/ml and acted on the MCF-7 cell to have, MTT test and rate of PR calculation.Results Compared with the blank control group(100%), when the concentration was 0.1 mg/ml, water elution part, 20% ethanol elution part and 60% ethanol elution part(98.10%, 101.06%, 106.04%)had no effect on the proliferation of MCF-7 cells, there was not statistically significant(P>0.05), while the 40% ethanol elution part(108.22%)can promote the proliferation of MCF-7 cells,there was a statistically significant(P<0.05). When the concentration was 1 mg/ml, 20%, 40% and 60% ethanol elution part(111.72%, 122.48%, 115.35%)can distinctly promote the proliferation of MCF-7 cells, there was a significant difference between four groups(P<0.01).ConclusionThe 40% ethanol elution part ofWujia-shenghua capsule has the strongest estrogen activity on plant.