Objective To observe the role of mesenteric lymphatic pathway in severe acute pancreatitis (SAP)by detection of inflammatory factors levels and mesenteric lymph duct ligation in rats.Methods Twenty-four SD male rats were randomly divided into group A (SAP group),group B (SAP + mesenteric lymph duct ligation group)and group C(sham operation group),with eight rats in each group.Samples of portal vein blood and mesenter-ic lymph were collected for measuring the level of tumor necrosis factor α(TNF-α),interleukin 1β(IL-1β),interleu-kin 6(IL-6)and endotoxin at twelfth hour.The blood CDl b was tested at sixth hour.24h lactulose /mannitol excretion ratios(L/M)were taken as the intestinal mucosal permeability index by gavage of 100mg lactulose and 50mg mannitol solution.The terminal ileums were collected for pathological examination.Results The blood levels of endotoxin and cytokines(TNF-α,IL-1β,IL-6)were significantly increased in group A compared with group C(all P <0.01).Each factor′s concentration in lymph was much higher than that in portal vein blood,especially in group A.The endotoxin and cytokines(TNF-α,IL-1β,IL-6)levels in portal vein blood of group B were significantly decreased compared with group A(all P <0.05),but still higher than that of group C(all P <0.05).CDl b expression in group A was the highest,followed by group B,group C was the lowest (P <0.01).24h L/M in group A was significantly higher than that in group C (P <0.01),and L/Min group B decreased significantly compared with those in A group(P <0.05). Conclusion Lymphatic pathway is an important pathway of systemic inflammatory response induced by SAP,and ligation of mesenteric lymph duct has a protective effect on the organism.

Objective To study the significance of combined detection of lung resistance protein(LRP),P-glycoprotein(P-gp),glutathione S-transferace-?(GST-?) and topoisomeraseⅡ(TopoⅡ) in gastric cancer prognosis judgment.Methods One hundred and thirty-six patients with human gastric carcinomas were studied for the expression of LRP,P-gp,GST-?and TopoⅡ by using immunohistochemistry.Results There were significant relationships in the positive expression rate of LRP,P-gp,GST-?between high-Medium differentiated adenocarcinoma and Poorly differentiated adenocarcinoma,Mucinous carcinoma,and Undifferentiated carcinoma(P

Objective To observe the clinical efficacy and adverse drug reactions of three dimensional-conformal radiotherapy combined with oral capecitabine in the treatment of stage Ⅱ , Ⅲ rectal cancer after operation. Methods Sixty-three patients of stage Ⅱ ,Ⅲ rectal eancer after operation with informed consent were divided into two groups by random digits table, 33 cases of observation group were treated by three dimensional-conformal radiotherapy combined with oral capecitabine, 30 cases of control group were treated by three dimensional-conformal radiotherapy only. Results The total effective rate in observation group was 84.8% (28/33), it was higher than that in control group [63.3% (19/30)], the difference between the two groups was statistically significant (P < 0.05). The occurrence rate of adverse reactions in two groups were compared, the difference was not statistically significant [57.6%(19/33) vs.50.0% (15/30)] (P>0.05). Conclusion Three dimensional-conformal radiotherapy combined with oral capecitabine in the treatment of stage Ⅱ,Ⅲrectal cancer after operation has satisfactory effect and acceptable adverse reaction, it is worth generalizing and applying.

Objective To investigate application of local anesthesia on the success rate of PICC. Methods A retrospective analysis was carried out in patients (120 cases)receiving PICC from March 2004 to January 2006. Randomly selected 120 cases were divided into 2 groups, each with 60 cases and PICC was im-plemented with the conventional method or under local anesthesia respectively in the two groups. Psychological changes of patients and nurses,adverse reactions of pain, success rate and incidence rate of complications were observed. Results The two groups were different in the following aspect, success rate, psychological changes, adverse reactions of pain,etc (P<0.01). Conclusions Local anesthesia during PICC is conducive to raise the success rate of tube insertion and the elimination of other adverse factors, especially for coarse PICC suite.

The primary immunotherapy for gastric cancer includes cytokine therapy,monoclonal antibody therapy, and cancer vaccines. A lot of researches are being conducted to study gene immunotherapy. It has found that Cbl-b gene plays an important role in regulating anti-tumor immunity and Cbl-b protein is involved in the lymph node metastasis of gastric cancer. Silencing Cbl-b gene in T lymphocytes can enhance anti-tumor immunity,therefore may become an effective treatment for patients with advanced gastric cancer.

Erythropoitin producing hepatocellular receptors(Ephs) and their ligands ephrins,the largest subset of tyrosine protein kinase receptor family,play a key role in the adhesion,orientation,migration and differentiation of cells.Eph and Ephrins are found closely correlated with tumorigenesis and development in human gastrointestinal tumors,especially colorectal cancer.The members of Ephs and ephrins show diverse expression in intestinal epithelial cells.Studying the mechanism and role of Ephs and ephrins on the malignant tumor occurrence and development will provide more accurate clinical basis for the treatment of colorectal cancer.

Abscess ; Anus Diseases ; Cough ; Fever ; Humans

Objective:To investigate the protective effect of resveratrol on lipopolysaccharide (LPS)-induced cell injury in human periodontal ligament cells(hPDLCs).Methods:hPDLCs were cultured and identified.The cultured hPDLCs were divided into 5 groups:control group and LPS(10 μg/ml) + RES(0/30,60 and 90 μmol/L respectively) groups.The cell proliferation was detected by MTI assay.The secretion of TNF-α and IL-6 of hPDLCs was detected by ELISA kit.The expression of TLR4/NF-κB mRNA and protein was determined by PCR and Western blot analyses,respectively.Results:The cultured cells were negative for cytokeratin and positive for vimentin staining.Compared with the control group,cell proliferation was decreased,the secretion of TNF-α/IL-6 levels and the expression of TLR4/NF-κB mRNA and protein were increased after treatment with LPS.Whereas,with 30-90 μmol/L resveratrol pretreatment,the proliferation ability of hPDLCs was enhanced(P ＜ 0.05),the secretion levels of TNF-α and IL-6 and the expression of TLR4/NF-κB mRNA and protein were reduced (P ＜ 0.01) in a dose-dependent manner.Conclusion:Resveratrol may attenuate LPSinduced cell injury by inhibiting TLR4/NF-κB pathway in hPDLCs.

In order to investigate the medical undergraduates' clinical practice teaching method and improve teaching quality we have adopted the teaching method by taking identification and diagnosis of the disease as focal point and various informative teaching means to the practice of Grade 2001 undergraduates of our academy.We have obtained the good result by increasing students' study interest and training students' the ability of analyzing and solving the problem.

Purpose:To summarize the base of electroacupuncture analgesia theory and the development of pain-reliever at present.Methods:By consulting a great deal of literatures,the mechanism of electroacupuncture analgesia is summarized from nerve,body fluid to molecule.The merits,kinds,using methods and notices of electroacupuncture are briefly introduced.Present research of Electroacuncture analgesia and its prospects based on traditional Chinese medical theory and modern science and technology are also summed up.Conclusion:This paper provides the basic theory for studying and developing the new style pain-relievers.