Objective To explore the levels of T lymphocyte subsets in asthmatic children and discuss their relationship.Methods 30 children with acute asthma attacks were divided ito disease control(bronchopneumonias)group(25 cases)and healthy control group(14 cases).Flow cytomerty was used to analyze the T lymphocyte subsets in them.Results CD 4+ and CD 4+/CD 8+T cell of the patients of the maderate to serere degree were both significantly higher than those in normal control.Conclusion(1)CD 4+ T cell plays an important role in asthma chronic airway inflammation.(2)There were no significant difference in the level of CD4+ T cell,CD8+ T cell,CD4+/CD8+ T cell between the patients with asthma of mild degree and those of moderate or severe degree group,but CD 8+ T cell a little higer in the latler patlents,which still needs further research.

With the development of translational research,many research institutes on translational research appeare around the world.These research institutes should focus on core problem,enhance the construction of information platform and training talent,evaluate translational projects and establish supporting system.Compared with foreign research institutes on translational medicine,the construction of Chinese research institute on translational research is in the initial stage,and many aspects need to improve.According to the current situation in China,we put forward that the research institutes on translational research in China should be settled in medical universities and promote the development of translational research by enhancing the training talents,developing the innovation of system,strengthening financing ability.

With the development of science and technology,many scientific achievements were gained,while a lot of problems emerged at the same time,for example,the expansion of gap between basic research and clinical research,the difficulty of clinical application of basic research achievement,all these promote the development of translational medicine.The appearance of translational research not only promote the formation of medical development strategy at the national level and the development of multiple disciplines,but also push the formation of the medical scientific research institutions on translational research and speed up the transformation of important achievements.Translational medicine in China is in the initial stage,and the top design and layout need to be increased through making overall coordinate and support,estabilishing a perfect training talent and the establishment of uniformed information platform,and so on.

Objective To explore the clinical significance of urine trace albumin in early diagnosis of diabetic nephropathy(DN). Methods A total of 35 patients with DN in DN group,32 patients with type 2 diabetes mellitus (DM)in DM group and 36 people accepted physical examination in control group.The urine trace albumin,blood urea nitrogen,serum creatinine were detected and compared.Results Urine trace albumin level in DN group was significant higher than those of DM group and control group,the differences were statistical significant(P <0.05),blood urea nitrogen and serum creatinine levels were slightly higher than those of DM group and control group,but there were no statistical significant differences (P >0.05).The urine trace albumin,blood urea ni-trogen and serum creatinine between DM group and control group had no statistical significant differences (P >0.05).Conclusion Urine trace albumin could be used as an early diagnostic index for early diagnosis of DN.

Androgen has broad biological effects. Androgen and its receptor exist widely in the cardio-cerebrovascular system and play an important protective effect in the multiple aspects of the occurrence and development of ischemic cerebrovascular disease (ICVD). In addition to involving in regulating the risk factors for ICVD, such as atherosclerosis,dyslipidemia, hypertension, coagulation-fibrinolysis abnormalities, obesity, and diabetes, androgen also has neuroprotective effect. This article reviews the protective effect of androgen in ICVD.

Objective To evaluate the efficiency of lamivudine combined with hepatitis B immunoglobulin to prevent hepatitis B recurrence after liver transplantation.Methods The literature concerning the application of lamivudine and hepatitis B immunoglobulin after liver transplantation was collected.The efficacy of initial lamivudine,and hepatitis B immunoglobulin alone or combined together was evaluated in liver transplantation recipients with hepatitis B by performing a systematic review of the literature with a Meta-analysis of clinical trials.Odds ratio(OR)was applied to evaluate the effect of therapeutic alliance to decrease the reinfection rate whether or not.Results We identified 7 clinical trials,and there were 360 patients subjected.OR and 95% confidence interval(95%CI)was 0.34(95%CI ranging from 0.18 to 0.64).For overall test result,Z value was 3.33 and P value was 0.01.The P value was 0.310 for our test of study homogeneity.Conclusion Our meta-analysis shows that lamivudine or hepatis B immunoglobulin can effective prevent hepatitis B recurrence after liver transplantation,and therapeutic alliance is more effective than monotherapy,and tolerance to lamivudine or hepatis B immunoglobulin was good.

A novel ionization enhancer, methylamine formate, was proposed for improving the detection sensitivity of triglyceride in edible oil by reversed phase liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS).The commonly used isopropanol-acetonitrile-methanol-water and isopropanol-acetonitrile were selected as the mobile phase.By using a reversed phase C18 column, and taking the corn oil in isopropyl alcohol as sample solution, we compared methylamine formate with ammonium formate, as ionization enhancers, for their effect on the detection sensitivity of triglyceride by LC-ESI-MS after screening other different ionization enhancers, such as formic acid, acetic acid, ammonium formate, ammonium acetate, butyl formate, dibutylamine formate, triethylamine formate, diethylamine formate, methylamine formate, and ethylamine formate.The result indicated that, by using methylamine formate, the mass spectral peak response and the signal to noise ratio for trilinoleic glyceride component were 5 times higher than that of ammonium formate.The effect of the concentration of methylamine formate ionization enhancer, the flow rate of the mobile phase and the flow rate of nebulizing gas on the detection of methylamine formate were investigated.The concentration of triglyceride components in corn oil starting to form aggregates was similar in different mobile phases in the electrospray process according to measurement of the relationship between corn oil concentrations and the total ion chromatogram peak area of triglyceride.In particular, the peak area of trilinolein was linear with its concentration in the range of 7×10-7-2×10-4 mol/L (R2=0.9997), but increased slower in the higher concentration range.According to the experimental data, the mechanism for improvement of detection sensitivity of methylamine formate was suggested as that the addition of methylamine mono-charged ions with hydrophobic groups had lower solvation energy, which made the enriched addition ions easily evaporation from the droplet surface, thus improving the electrospray ionization efficiency.This method provided an effective way to improve the detection sensitivity of triglyceride in edible oil by LC-ESI-MS.

Objective To investigate the expression of microRNA -216a(miR -216a)in patients suf-fering with breast cancer ,and identify the function and mechanism of miR -216 a on autophagy .Methods The expression of miR-216 a in 30 tumor tissues and paired normal tissue of breast cancer were detected by qRT -PCR.Inhibiton of miR-216a in MCF-7 cell lines was done by transfection of miR -216a inhibitor ( AMO-216a).Cell viability was detected by MTT assay .The level of Beclin 1 was detected by western blot .Results The level of miR-216 a was significant elevated in tumor tissue .Cell viability was markedly decreased owing to inhibition of miR-216a in MCF-7 cells.The level of Beclin 1 was significantly increased by transfection of miR-216a inhibitor in MCF-7cells(P<0.05).Conclusion The level of miR-216a is increased in breast canc-er tissue, and it might at least in part promote breast cancer via downregulating Beclin 1 and affecting autophagy .

Disease bio-banks are an important strategic resource for medicine development. Such a bank with complete information and high information sharing enhances the competitiveness of medicine and promote the development of translational medicine. The present development of such banks is found with such setbacks as weak government leadership and standardization, as well as lack of a sharing mechanism. Therefore medical institutions should strengthen their standardization and informationization of disease bio-banks, which deserve sufficient government policy support and guidance. These efforts will help preserve our rich clinical resources, in addition to their integration and sharing for medicine advancement.

Objective To describe and explore the practice of eye care in ICU,in order to make a specified training plan and guideline on eye care,and to improve the skill in eye care of ICU nurses. Methods A survey was conducted in 92 ICU nurses from 2 general hospitals in Huizhou city with non-random sampling using a self- made questionnaire to investigate their eye care of patients. Results The nursing practice of eye care in ICU was urged to be improved,there was no standard on eye care. Conclusions The knowledge of ICU nurses needs to be enhanced,and guideline on eye care is urged to be worked out,so that practice of eye care would be improved.