The feasibility and safety of non - operative treatment of splenic rupture were assessed experimentally in rats.All the experimental animals survived except that there was one death in the group with complete laceration of all the layers of the spleen and in the group with comminuted rupture of the spleen respectively.Non-operative treatment was used to treat 7 cases of single rupture of the spleen with satisfactory results.These findings suggest that non -operative treatment is an effective and safe procedure for the management of blunt injury of the spleen when hemodynamics of the patient is stable.

Objective To compare the diagnostic values of different parameters for neonatal sepsis by observing clinical presentations and laboratory tests of hospitalized newborn infants and to find out the the early and fast way in neonatal sepsis diagnosis. Methods Newborn infants admitted to NICU from Sept.2007 to Feb.2008,who met all inclusion and exclusion criteria,were enrolled.The situations in perinatal period,exact time of the onset of infection,clinical manifestations and laboratory indexes were recorded.Infants were classified into 3 groups:septic group(n=13),nonseptic group(n=12) and noninfectious group(n=12).Sensitivity,specificity,false positive rate,false negative rate,Youden's index and positive and negative predictive values(PPV and NPV) were calculated for each test. Receiver-operating characteristic curves were analyzed to determine the optimal thresholds. Results Among all the clinical manifestations,abnormal reaction had the hightest specificity(84.6%) and accuracy(67.9%) in diagnosing neonatal sepsis.SIRS criteria,with the specificity of 95.8%,was helpful in clinical suspected eases.However,routine laboratory tests,such as WBC,PLT and GLU,had poor sensitivity or specificity;I/T had better specificity(100%),but was subject to discrepancy among different observers.The area under the ROC for CRP and PCT were 0.734 and 0.878,with the optimal threshold of 15 mg/L(P=0.020,Youden's index=45.5%) and 0.81 ng/ml(P-0.000,specificity=100%,Youden's index=75.0%),respectively.Conclusions Careful clinical inspection warrants early detection of sick infants.Evaluation of SIRS criteria in clinical suspected septic infants may help in early diagnosis.WBC,I/T,PLT can be used as routinely monitoring indexes for high risk neonates.CRP and PCT concentrations also have significant diagnostic values.

Objective:To investigate the effects of soluble factors secreted by MCF-7 breast cancer cells on the differentiation,maturation and function of DCs.Methods:Mononuclear cells were cultured with the culture supernatants from primary MCF-7 cells.Combination of granulocyte-macrophage colony stimulating factor(GM-CSF),interleukin 4(IL-4) and tumor necrosis factor-a(TNF-a) was used to stimulate the cultured mononuclear cells.Then DCs and CTL assays were analyzed.Results:MCF-7 cell supernatant-treated DCs resulted in low expression of CD80,CD83,CD86 and HLA-DR,and the inhibition rate of CTL was 17.35% significantly lower than 56.14% induced by the control DCs(P＜0.01).IL-12 secreted by DCs and IFN-γ produced by PBMNC were all significantly lower than those in the control group (P＜0.01).Conclusion:Experiments in vitro shows that the culture supernatants from MCF-7 breast cancer cells could inhibit the development and functions of DCs.

Tumor is one of the most serious problems threatening people's life in the 21 st century. Antitumor vaccine becomes a hot research spot of tumor therapy for low toxicity, specificity and durability. Efficient recognization and presentation of tumor antigen contribute to the foundation of an powerful anti-tumor immunologic response. Along with the deepen apprehension of the immunity mechanism and high development of the biochemical technology, a great number of new vaccines emerge and show us some favors. And DNA vaccine and DC vaccine attract most sights for their advantage in antigens expression and presentation.They become the strong weapon for tumor immunity therapy. This is a review about the mechanism, development and current problem of DNA vaccine and DC vaccine.

Objective　To investigate the changes of CEA levels of blood, ascites and cancer tissue in nude mice with liver metastases from colon adenocarcinoma treated by 131I-labeled anti-CEA monoclonal antibody (McAb). Methods　131I-labeled anti-CEA McAb was injected through tail vein at 10min(early treatment group), 10d(middle treatment group) and 20d(late treatment group) after the liver metastases were established respectively. 131I-labeled anti-normal mice IgG was injected through tail vein after the liver metastatic model was estabished as a control group. Results　The CEA levels of blood, ascites and cancer tissue in early treatment group were significantly decreased compared with the control group (P<0.01); but, there was no difference between the early treatment group and the late treament group (P>0.05). Conclusions　The CEA levels of blood, ascites and cancer nodulus in nude mice with liver metastases from colon cancer could be lowered significantly when the 131I-labeled anti-CEA McAb is used early after the liver metastasis is established.

Objective To investigate the changes of CEA levels of blood, ascites and cancer tissue in nude mice with liver metastases from colon adenocarcinoma treated by 131 I labeled anti CEA monoclonal antibody (McAb). Methods 131 I labeled anti CEA McAb was injected through tail vein at 10min(early treatment group), 10d(middle treatment group) and 20d(late treatment group) after the liver metastases were established respectively. 131 I-labeled anti-normal mice IgG was injected through tail vein after the liver metastatic model was estabished as a control group. Results The CEA levels of blood, ascites and cancer tissue in early treatment group were significantly decreased compared with the control group (P0.05). Conclusions The CEA levels of blood, ascites and cancer nodulus in nude mice with liver metastases from colon cancer could be lowered significantly when the 131 I labeled anti CEA McAb is used early after the liver metastasis is established.

OBJECTIVE To investigate the distribution of infectious diseases and diagnostic and therapeutic conditions in pediatric department of a general hospital,and try to find out the measures to decrease hospital infection.METHODS From Jan 2004 to Dec 2005,507 cases of infectious diseases were retrospectively analyzed in pediatric department of our hospital.RESULTS Among them epidemic parotitis,infectious diarrhea and bacillary dysentery were 56.2%,20.1%,and 17.4%,respectively.Others were measles,scarlet fever,rebella,viral hepatitis,epidemic cerebrospinal meningitis,typhoid,and syphilis.Among the cases,the most were outpatients.CONCLUSIONS Because the species of infectious diseases are various and the amount are large,the pediatric department is important in a general hospital for management of hospital infection.Strengthening the standardized management of pediatric infectious diseases and taking strong measures may play the important role in control the cross infection.

Objective To investigate the influence of laparoscopic resection of colorectal cancer on (postoperative) micrometastasis of tumor cells.Methods Using cytokeratin,CK-20 mRNA as target gene,the RT-PCR method was used to examine the pre-and post-operative changes in the number of tumor cells in the peripheral blood of 48 cases of colorectal cancer.Results After operation,the number of tumor cells in(peripheral) blood was significantly higher in the laparoscopic surgery group as compared to that of traditional(laparotomy) operation group(P0.05),but was significantly related to positive rate of proliferating cell nuclear antigen(PCNA)(P

AIM: To study the influence on the expression of proliferation cell nuclear antigen(PCNA) in rectal carcinoma through retention -enema with FUDR and 5 -FU. METHODS: 80 cases of rectal carcinomas were randomized into three groups: the FUDR group(30 cases), the 5-FU group(30 cases) and normal control group(20 cases), treated with FUDR(500mg), 5-FU(500mg) and normal saline(20ml ) everynight through retention - enema separately for serven days- Mucosa of rectal car cinoma were sampled and PCNA protein were simultaneously detected by imrnunohistochemical method on the first and eighth day. RESULTS: The expression of PCNA was significantly decreased in both FUDR group and 5 - FU group after treatment(P 0. 1). CONCLUSION:The cell proliferation of rectal carcinoma could be inhibited by FUDR and 5-FU through retention-enema. The effects of FUDR was more obvious than that of 5-FU in the same concentration. so the treating method through retention-enema with FUDR should be used as a routine therapeutic scheme befOre operation.

Objective To investigate the indications and suitable surgical operation routes of total gastrectomy for gastric carcinoma and the reconstruction procedure of digestive tract following total gastrectomy.Methods The clinical data of total gastrectomy by abdominal incision in 170 patients with GC from 1991 to 2001 were reviewed.Results Radical total gastrectomy was prfomed in 132 cases,palliative total gastrectomy in 38 cases,total gastrectomy with combined re section of other organs in 18 cases,Roux en Y esophagojejunostomy in 110 cases following total gastrectomy.Interposition with jejunum in 60 cases following total gastrectomy.There are 20 cases with dumping syndrome and 6 cases with reflux esophagitis occurring in Roux en Y esophagojejunostomy,but none of cases occurs in those by interposition with jejunum.Conclusions (1)Transabdominal incision is the better choice for patients of GC,especially gastrocardiac carcinoma.(2)The total gastrectomy can raise the survival rate and quality of life of patients with GC,if the indications are stricted.(3)Interposition with jejunum following total gastrectomy is superior to Roux en Y esophagojejunostomy.