Objective To explore the difference of efficacy between LEEP and microwave treatment on chronic cervicitis.Methods According to the different treatment method,400 patients with chronic cervicitis were divided into LEEP group (LEEP treatment) and microwave group (microwave treatment),each group 200 cases.The treatment time,amount of bleeding and the healing effect between the two groups were compared and analyzed.Results The operative time,amount of bleeding before and after operation,vaginal fluid time after surgery of LEEP group were (6.94 ± 1.13) min,(2.13 ± 0.32) ml,(15.23 ± 3.40) mi,(7.06 ± 1.47) d,which were better than those of the microwave group[(16.13 ± 2.81) main,(5.94 ± 1.45) ml,(50.06 ± 7.86) ml,(17.58 ± 4.33) d] (t =42.91、36.29、57.52、32.54,all P ＜ 0.05).The number of recovery patients had no statistically significant differences in mild,moderate patients of the two groups(97 cases vs 104 cases and 54 cases vs 45 cases,χ2 =0.81,1.35,all P ＞0.05),and the number of recovery patients in the LEEP group was better than that in the microwave group(23 cases vsl4 cases,χ2=6.87,P ＜ 0.05).Conclusion The efficacy of LEEP knife on chronic cervicitis was significantly.It has a less operative time,less blood loss,better recovery.It is worthy of promotion.

Toll-like receptor is a receptor to identify the pathogen associated molecular,which relates the natural immune with required immune.Toll-like receptor activates natural immune and induces several cytokines.As a result,it will influence the differentiation of initiated T-cell by identifying Pathogens.The main Tolllike including TLR2、3、4、7、8、9 are closely related with anti-rotavirus to identify the natural immune affection of rotavirus.This review to explain the pathogenesis of Toll-like in rotavirus infection,as well as the key role which Toll-like plays in preventing rotavirus infection and controlling the inflammation.

Objective To investigate the change of homocysteine level,vascular endothelial growth factor (VEGF) and adiponectin levels in type 2 diabetic patients with diabetic retinopathy and its signicance.Methods 60 patients with diabetic retinopathy ( DR group) and 40 patients with normal fundus ( NDR goup) were selected.30 healthy subjects were used as control group.The serum levels of Hcy,VEGF and adiponectin were detected.Results The serum levels of Hcy and VEGF of DR group and NDR goup patients were higher than that of control group and had statistical significance(all P ＜0.05) ;The serum levels of Hcy and VEGF of DR group were higher than that of NDR group( all P ＜ 0.05 ).The serum level of adiponectin of DR group and NDR goup patients was lower than that of control group ( all P ＜ 0.05 ) ; The serum level of adiponectin of DR group was lower than that of NDR group ( all P ＜ 0.05 ).Hcy and VEGF had a positive correlation with adiponectin( all P ＜ 0.05 ).Conclusion Hcy,VEGF and adiponectin may play important roles in the initiation and sustainment of DR.

BACKGROUND: The effect and mechanism of peroxisome proliferator-activated receptor gamma (PPARγ) agonists on transforming growth factor (TGF)-β1-induced fibrotic responses in lung fibroblasts remain unclear, and seldom reported.OBJECTIVE: To study the effects of PPARγ agonists on TGF-β1-induced fibrotic responses in lung fibroblasts, so as to investigate its effects in preventing pulmonary interstitial fibrosis of silicosis. METHODS: The pulmonary alveolar macrophage was prepared with 200 mg/L SiO2 and the supernatants were got. Chinese hamster lung fibroblasts were incubated with the prepared supernatants. Then a cellular model in vitro for studying pulmonary fibrosis induced by SiO2 was developed. Chinese hamster lung fibroblasts were cultured to observe the effects of the PPARγ ligand (15 d-PGJ2) and its agonists (troglitazone and ciglitazone) on the expression of TGF-β1-induced fibronectin. RT-PCR was used to detect the mRNA expression of fibronectin and Western blotting was used to detect the protein expression of fibronectin. RESULTS AND CONCLUSION: (1) The expression of TGF-β1 mRNA showed an effect in a dose- (0-5 μg/L) and time- (0-24 hours) dependent manner. (2) PPARγ ligands and agonists reduced the mRNA and protein expressions of fibronectin. Results suggested that PPARγ agonists can inhibit TGF-β1-induced fibronectin synthesis after stimulation by SiO2 and may play a potential role in preventing pulmonary interstitial fibrosis.

Objective To observe the clinical efficacy and adverse drug reactions of three dimensional-conformal radiotherapy combined with oral capecitabine in the treatment of stage Ⅱ , Ⅲ rectal cancer after operation. Methods Sixty-three patients of stage Ⅱ ,Ⅲ rectal eancer after operation with informed consent were divided into two groups by random digits table, 33 cases of observation group were treated by three dimensional-conformal radiotherapy combined with oral capecitabine, 30 cases of control group were treated by three dimensional-conformal radiotherapy only. Results The total effective rate in observation group was 84.8% (28/33), it was higher than that in control group [63.3% (19/30)], the difference between the two groups was statistically significant (P < 0.05). The occurrence rate of adverse reactions in two groups were compared, the difference was not statistically significant [57.6%(19/33) vs.50.0% (15/30)] (P>0.05). Conclusion Three dimensional-conformal radiotherapy combined with oral capecitabine in the treatment of stage Ⅱ,Ⅲrectal cancer after operation has satisfactory effect and acceptable adverse reaction, it is worth generalizing and applying.

Objective　To evaluate the value of ultrasound in the diagnosis of benign and malignant ovarian teratomas.Methods　The ultrasonographic imaging features of eighty-four cases of benign and malignant ovarian teratomas were viewed by two dimensional and color Doppler ultrasonography.Results　Eighty-four cases were confirmed by pathology report that seventy-eight cases were ovarian teratomas.The accurate rate of ultrasound diagnosis was 92.9%(78/84).Among these cases,tumors which on the single side existed in sixty-nine cases (two tumors existed on the same side in two cases),and which on double sides existed in nine cases.Eighty-nine tumors were checked out all together.The ultrasonographic images of the ovarian teratomas displayed three types: mix(65 masses) , false solid (14 masses) and cyst (10 masses) type. Mix type was the major (occupied 65/89,73.0%). Multiplicate signs are shown in all of them. Malignant teratomas (3 masses,4 masses) not only appeared similar,but also more complex than the benign.Conclusions　Ultrasonography is the first examination to be selected to diagnose teratoma of the ovary. Their ultrasonographic images are charaeteristic. If the images appears disorderly inside, the malignancy should be predicted with other clinical materials.

Highly developed molecular genetic techniques have got implemented widely in the diagnosis of birth defects and genetic diseases.Due to the special nature of genetic testing , there is still a lack of appropriate external quality assessment , but many clinical laboratories are conducting or going to conduct genetic diagnosis for monogenic diseases and prenatal diagnosis.In this paper , it will be discussed that how to ensure the quality control of the laboratory diagnosis of monogenetic diseases , based on the present application of genetic testing technologies and the difficulties in the diagnosis of monogenetic diseases.

Objective To investigate the application of the cytochrome C oxidase subunit Ⅰ gene(COXⅠ ) barcoding in the i‐dentification of wild‐caught ticks .Methods The COXⅠ gene of 9 caught tick individuals with different species was sequenced ,the DNAstar software was used to conduct the sequence comparison and homology analysis .Meanwhile the philp3 .6 software was a‐dopted to select the maximal likelihood(ML) method for constructing the evolutionary trees .Results In the phylogenetic trees ,the samples of sh2011040702 ,ra2011042193 and ra2011051168 with Haemaphysalis were in a branch ,sh2011051404 ,sh2011051408 and ra2011041177 with dermacentor were clustered together ,and sh2011051837 ,sh2011041917 and ra2011041175 with hyalomma were in a branch ,which were consistent with the morphological identification results .Conclusion Making the COXⅠ gene sequence as tick DNA barcoding has certain feasibility for identifying the tick species .

Objective To observe the clinical efficacy of moxibustion in treating primary dysmenorrhea in adolescent female undergraduates. Method Sixty patients with primary dysmenorrhea were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by moxibustion at Guanyuan (CV 4), while the control group was by moxibustion at Sanyinjiao (SP 6). The symptom score was observed before intervention and after 3 menstrual cycles, and the clinical efficacies were compared. Result The symptom score was changed significantly in both groups after intervention (P<0.01). After intervention, there was a significant difference in comparing the symptom score (P<0.05). The total effective rate was 100.0%in the treatment group versus 93.3% in the control group, and the difference was statistically insignificant (P>0.05). Conclusion Moxibustion is an effective approach in treating primary dysmenorrhea, and moxibustion at different points will produce different effects.

Objective To understand the epidemic situation of common human soil-transmitted nematode infections in Quan-zhou City in 2012,so as to provide the evidence for improving the prevention and control measures. Methods The residents aged above 3 years from 5 townships in 2 counties were investigated to detect the parasite eggs(hookworm,Ascaris lumbricoides,Trich-uris trichura)by Kato-Katz technique. One kindergarten of each county was randomly selected and 228 children were investigated to detect Enterubius vermicularis eggs by the cellophane tape anus test. Results The total hookworm infection rate was 3.60%(69/1919). For the hookworm infection rates,there was no significant difference among different areas and between different genders, but there were significant differences among different age groups and different careers. No Enterubius vermicularis eggs were detect-ed. Conclusion The effect of the prevention and control of Ascaris lumbricoides,Trichuris trichura and Enterubius vermicularis in-fections is remarkable. However,the infection rate of hookworm has a rising trend and the prevention and control of hookworm in-fection should be strengthened.