BACKGROUND:Several studies have confirmed that bone marrow mesenchymal stem celltransplantation exerts a certain neuroprotective role in cerebral infarction. Edaravone is a novel potent smal-molecule hydroxyl radical scavenger, which play a protective role in the brain by eliminating free radicals and inhibiting nerve celldamage after cerebral infarction. OBJECTIVE:To explore the influence of bone marrow mesenchymal stem cells combined with edaravone on expressions of aquaporin-4, Bcl-2, and brain-derived neurotrophic factor after cerebral infarction in rats. METHODS:Eighty Wistar rats were selected to establish models of cerebral infarction by right middle cerebral artery occlusion, and then randomly divided into control group, bone marrow mesenchymal stem cells group, edaravone group and edaravone+bone marrow mesenchymal stem cells group (combination group). After 6 hours of modeling, rats from these four groups were respectively injected via the tail vein with PBS, bone marrow mesenchymal stem cells, edaravone and edaravone+bone marrow mesenchymal stem cells. After 72 hours, the rats were decapitated to take brain tissues. Consequently, expressions of aquaporin-4, Bcl-2, and brain-derived neurotrophic factor mRNA and protein were determined by RT-PCR and western blot methods. After 12, 24 and 36 hours, TUNEL method was used for the determination of cellapoptosis. RESULTS AND CONCLUSION:The expressions of Bcl-2 and brain-derived neurotrophic factor were higher in the combination group than the other three groups (P<0.05), but the expression of aquaporin-4 was lowest in the combination group (P<0.05). The number of apoptotic cells in the combination group was significantly lower than that in the other three groups. These findings suggest that the combination of bone marrow mesenchymal stem cells and edaravone can improve expressions of Bcl-2 and brain-derived neurotrophic factor in the injured site, and significantly inhibit neuronal apoptosis. Meanwhile, the combination therapy can decrease expression of aquaporin-4 and mitigate cerebral edema, which is superior to bone marrow mesenchymal stem celltransplantation and edaravone alone.

Objective To observe the clinical efficacy of moxibustion in treating primary dysmenorrhea in adolescent female undergraduates. Method Sixty patients with primary dysmenorrhea were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by moxibustion at Guanyuan (CV 4), while the control group was by moxibustion at Sanyinjiao (SP 6). The symptom score was observed before intervention and after 3 menstrual cycles, and the clinical efficacies were compared. Result The symptom score was changed significantly in both groups after intervention (P<0.01). After intervention, there was a significant difference in comparing the symptom score (P<0.05). The total effective rate was 100.0%in the treatment group versus 93.3% in the control group, and the difference was statistically insignificant (P>0.05). Conclusion Moxibustion is an effective approach in treating primary dysmenorrhea, and moxibustion at different points will produce different effects.

Highly developed molecular genetic techniques have got implemented widely in the diagnosis of birth defects and genetic diseases.Due to the special nature of genetic testing , there is still a lack of appropriate external quality assessment , but many clinical laboratories are conducting or going to conduct genetic diagnosis for monogenic diseases and prenatal diagnosis.In this paper , it will be discussed that how to ensure the quality control of the laboratory diagnosis of monogenetic diseases , based on the present application of genetic testing technologies and the difficulties in the diagnosis of monogenetic diseases.

BACKGROUND: The effect and mechanism of peroxisome proliferator-activated receptor gamma (PPARγ) agonists on transforming growth factor (TGF)-β1-induced fibrotic responses in lung fibroblasts remain unclear, and seldom reported.OBJECTIVE: To study the effects of PPARγ agonists on TGF-β1-induced fibrotic responses in lung fibroblasts, so as to investigate its effects in preventing pulmonary interstitial fibrosis of silicosis. METHODS: The pulmonary alveolar macrophage was prepared with 200 mg/L SiO2 and the supernatants were got. Chinese hamster lung fibroblasts were incubated with the prepared supernatants. Then a cellular model in vitro for studying pulmonary fibrosis induced by SiO2 was developed. Chinese hamster lung fibroblasts were cultured to observe the effects of the PPARγ ligand (15 d-PGJ2) and its agonists (troglitazone and ciglitazone) on the expression of TGF-β1-induced fibronectin. RT-PCR was used to detect the mRNA expression of fibronectin and Western blotting was used to detect the protein expression of fibronectin. RESULTS AND CONCLUSION: (1) The expression of TGF-β1 mRNA showed an effect in a dose- (0-5 μg/L) and time- (0-24 hours) dependent manner. (2) PPARγ ligands and agonists reduced the mRNA and protein expressions of fibronectin. Results suggested that PPARγ agonists can inhibit TGF-β1-induced fibronectin synthesis after stimulation by SiO2 and may play a potential role in preventing pulmonary interstitial fibrosis.

Objective To observe the clinical efficacy and adverse drug reactions of three dimensional-conformal radiotherapy combined with oral capecitabine in the treatment of stage Ⅱ , Ⅲ rectal cancer after operation. Methods Sixty-three patients of stage Ⅱ ,Ⅲ rectal eancer after operation with informed consent were divided into two groups by random digits table, 33 cases of observation group were treated by three dimensional-conformal radiotherapy combined with oral capecitabine, 30 cases of control group were treated by three dimensional-conformal radiotherapy only. Results The total effective rate in observation group was 84.8% (28/33), it was higher than that in control group [63.3% (19/30)], the difference between the two groups was statistically significant (P < 0.05). The occurrence rate of adverse reactions in two groups were compared, the difference was not statistically significant [57.6%(19/33) vs.50.0% (15/30)] (P>0.05). Conclusion Three dimensional-conformal radiotherapy combined with oral capecitabine in the treatment of stage Ⅱ,Ⅲrectal cancer after operation has satisfactory effect and acceptable adverse reaction, it is worth generalizing and applying.

Objective　To evaluate the value of ultrasound in the diagnosis of benign and malignant ovarian teratomas.Methods　The ultrasonographic imaging features of eighty-four cases of benign and malignant ovarian teratomas were viewed by two dimensional and color Doppler ultrasonography.Results　Eighty-four cases were confirmed by pathology report that seventy-eight cases were ovarian teratomas.The accurate rate of ultrasound diagnosis was 92.9%(78/84).Among these cases,tumors which on the single side existed in sixty-nine cases (two tumors existed on the same side in two cases),and which on double sides existed in nine cases.Eighty-nine tumors were checked out all together.The ultrasonographic images of the ovarian teratomas displayed three types: mix(65 masses) , false solid (14 masses) and cyst (10 masses) type. Mix type was the major (occupied 65/89,73.0%). Multiplicate signs are shown in all of them. Malignant teratomas (3 masses,4 masses) not only appeared similar,but also more complex than the benign.Conclusions　Ultrasonography is the first examination to be selected to diagnose teratoma of the ovary. Their ultrasonographic images are charaeteristic. If the images appears disorderly inside, the malignancy should be predicted with other clinical materials.

Toll-like receptor is a receptor to identify the pathogen associated molecular,which relates the natural immune with required immune.Toll-like receptor activates natural immune and induces several cytokines.As a result,it will influence the differentiation of initiated T-cell by identifying Pathogens.The main Tolllike including TLR2、3、4、7、8、9 are closely related with anti-rotavirus to identify the natural immune affection of rotavirus.This review to explain the pathogenesis of Toll-like in rotavirus infection,as well as the key role which Toll-like plays in preventing rotavirus infection and controlling the inflammation.

The primary immunotherapy for gastric cancer includes cytokine therapy,monoclonal antibody therapy, and cancer vaccines. A lot of researches are being conducted to study gene immunotherapy. It has found that Cbl-b gene plays an important role in regulating anti-tumor immunity and Cbl-b protein is involved in the lymph node metastasis of gastric cancer. Silencing Cbl-b gene in T lymphocytes can enhance anti-tumor immunity,therefore may become an effective treatment for patients with advanced gastric cancer.

Objective To summarize the perioperative nursing cares for patients with coronary in-tervention by transraidal approach. Methods Retrospective analyzed the nursing cares among 42 pa-tients with coronary intervention by transraidal approach. Results Normal postoperative radial plnse were monitored in 42 patients, there were no flase aneurysm, arterio-venous fistula, median nerve injury and dysporia occurred. Conclusions Coronary intervention by transraidal aporoach can reduce perioperative hemmorrhage, avoid certain related complications and saving financial costs. Renforee preoperative psychological cares can re-lease pains of patients and then promote the nursing quality.

Abdomen ; anatomy & histology ; Acupuncture Points ; Acupuncture Therapy ; Adult ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Leiomyoma ; therapy ; Middle Aged