Objective:To verify the applicability of Dynamic Contrast Enhanced Magnetic Resonance Imaging(DCE-MRI)to evaluate the breast cancer therapy using the high intensity focused uhrasound(HIFU).Methods:Both DCE-MRI and conventional T_1W/T_2W images were acquired for the total 8 subjects.Two consecutive scans were performed for every patient(before operation and 2 weeks after operation)while four were performed for only 2 patients(additional for 6 months and 12 months after operation).The breast cancer therapy by HIFU was assessed hy comparing the changes of signal,the dynamic patterns of time-signal intensity curve,the early-phase contrast enhancement ratios and vessel anatomies around cancer focuses.Results: The shortened T_2 signals were observed in cancer focuses of 5 subjects while 3 still showed longer T_2 values which were differentiated by the high intensity in T_2W images.The early-phase contrast enhancement ratios of those 5 subjects decreased from 60%～80% to

Objective To explore the risk management factors and preventive measures in obstetric care. Methods The implementation of risk management in our hospital was retrospectively analyzed. Results Maternal satisfaction degree was significantly increased, complaints and errors of nursing care were significantly decreased. Conclusions Strengthening of risk management in obstetric care can benefit to eliminate and prevent unsafe factors, so that obstetric care quality is improved.

Objective To study the effect of letrozole plus metformin on infertility of polycystic ovary syndrome(PCOS).Methods According to the digital table,90 patients with infertility of PCOS were randomly divided into the control group(n =45 cases) and the treatment group(n =45 cases).The patients of the control group were treated though clomiphene,while the patients of the treatment group were treated though letrozole plus metformin.The ovulation rate,pregnancy rate,sex hormones and insulin resistance were detected.Results The homeostasis model assessment of insulin resistance index (HOMA-IR) was (5.72 ± 2.37) before treatment and (3.16 ± 1.39) after treatment in the treatment group.HOMA-IR was (5.69 ± 2.40) before treatment and (4.81 ± 2.06) after treatment in the control group.There was a significantly decrease in the treatment group (t =3.7246,P ＜ 0.05).The ovulation rate was 80％ in the treatment group,while 60％ in the control group,the difference between the two groups was significant(x2 =4.06,P ＜0.05).The pregnancy rate was 48.9％ in the treatment group,while 28.9％ in the control group,the difference between the two groups was significant (x2 =5.77,P ＜ 0.05).Conclusion Letrozole plus metformin could promote the ovulation and decrease insulin resistance.

Objective To detect the expression of the vascular endothelial growth factor (VEGF) and its receptors, the fms like tyrosine (flt 1), kinase insert domain containing receptor (KDR) in normal human endometrium during menstrual cycle and investigate the functions of VEGF and its receptors for development and differentiation of human endometrium Methods Immunohistochemistry and in situ hybridization techniques were used to measure the level of VEGF, flt 1 and KDR protein and mRNA in normal endometrium from 50 women Few samples of endometrium respectively in the proliferative and secretory phase were homogenized for Western Blot analysis of VEGF subtypes The blood vessel density was estimated by counting staining with a endothelial marker Ⅷ factor Results The VEGF and its receptors mainly expressed in endometrial endothelial cells and gland epithelium The level of VEGF, protein and mRNA, as well as flt 1 in mid secretory and menstrual phase were highest ( P 0 05) Western Blot analysis showed stained VEGF bands in 34 000 (VEGF 121 ), 46 000 (VEGF 165 ), 54 000 (VEGF 189 ), 68 000 (VEGF 206 ), predominantly in 34 000 and 46 000 Conclusions VEGF, flt 1 and KDR, including both protein and mRNA, showed a pronounced menstrual cycle dependent expression in cycling endometrium VEGF and its receptors expressing higher in secretory phase and menstruation probably be involved in embryonic implantation and endometrial shedding

Objective To investigate the relationship between human albumin stimulation and MCP-1 production of human renal tubular epithelial cells. Methods Thirteen patients with minimal change disease (MCD) were enrolled into the study. MCP-1 expression and M? infiltration in renal biopsy tissues were studied by immunohistochemistry (IHC) and in situ hybridization (ISH). In vitro experiment, proximal tubular epithelial HK-2 cells were stimulated by human serum albumin in different concentration, then MCP-1 production was detected by Western Blot. Results In renal tissues of MCD, focal positive MCP-1 was found in tubular walls and a few M infiltrated in every area including tubular walls , meanwhile a correlation of M? infiltration between interstitium and tubular lumen was studied. In cell culture experiment, with its concentration more than 2 g/L. human serum albumin could stimulate HK-2 cells to produce MCP-1. Conclusions MCP-1 expresses in the tubules of MCD patients with massive proteinuria. A few M?infiltrate in the renal tissue and move between the interstitium and the lumen of tubules. Human serum albumin can stimulate human tubular epithelial cells to produce MCP-1.

Objective To identify the relationship among chronic inflammatory state, malnutrition and cardiovascular diseases in peritoneal dialysis patients. Methods Cardiovascular diseases and dialysis regime in 90 clinically stable continuous ambulatory peritoneal dialysis (CAPD) patients were investigated. Then, dietary energy intake (DEI) and protein intake (DPI) through dietary diaries were measured. The serum albumin (Alb), prealbumin(PA), transferring (TF), lean body mass (LBM), LBM% and the normalized protein equivalent of total nitrogen appearance (nPNA) were examined or calculated. Subjective global assessment (SGA) was also evaluated. Serum interleukin-6 (IL-6), tumor necrotic factor-?(TNF-?) and C-reactive protein (CRP) were measured as markers of chronic inflammation. Serum leptin and plasma NPY were detected too. Results Serum IL-6 and TNF-? levels of CAPD cases were (17. 17?27.72) pg/ml and (34. 21?25. 92) pg/ml, which were significantly higher as compared to control group. Serum CRP level in CAPD patients was (9. 88?20. 93)mg/L. There were 24 patients(26. 67% ) with CRP above normal level. 55 patients(61. 11% ) had cardiovascular diseases. The patients with angina pectoris, old myocardial infarction and chronic heart failure had higher CRP levels than those without above complications ( P

Objective To analyze distribution and drug resistance of MRSA separated,to probe into a treat method for MRSA infection and offer scientific gist for reasonable use of clinical antibiotic. Methods 568 MRSA separated from clinic were measured and a drug sensitivity test was performed by means of Kirby-Bautr agar diffusion method. Results 370 individual plants were filtered, the rate of inspection was 65.1%. MRSA was 36.2 % among them,MRSCON was 77.5% ,the drug resistance of MRSA to quinolones(21% ～48% ) was below MRSCON(84 %～89 % ). The drug resistance of MRSA and MRSCON obviously excelled MSSA and MSSCON ( P＜0.05 ). VRS was undiscovered. Conclusion Severe infection caused by MRS should choose vancomycin resistant enterococci to cure at fist. Staphylococcus was one of primary pathogenic bacterium caused infection in hospital, MRS had multiple drug resistance and measure range about drug resistance should be extended, which would advance the cognition about the drug resistance of antibiotic and cut off the transmitted path, reduce the drug resistance of bacteria and the infection rate in hospital.

Objective To acknowledge the psychological authority status under the foundation of autho0rization management,in order to analyze the effectiveness of authorization management.Methods 364 clinical nurses were investigated with Psychological Empowerment Scale and the results were performed statistic analysis through descriptive statistical method and variance analysis.Results The average score of clinical nurses was(3.22±0.70),which was at the moderate point.The four dimensional scores listed in descending order were self efficient,career significance,autonomy and job influence.Clinical nurses of different ages,different ranking,different working period,different marriage status had statistical outstanding variance.But nurses of different education background showed no significant difference in score of each dimension.Conclusions The nursing management shall not only focus on the authorization management itself,but also on the psychological authority status of clinical nurses.Through an efficacious incentive,we can enhance the psychological recognition and experience on authorization and activate the working motivation,maximize the working initiative and thus promote the innovation and expansion of nursing work.

Chronic kidney disease (CKD) has become a public health issue worldwide.A recent cross-sectional survey revealed that the prevalence of CKD in residents aged 18 years or older was 10.8％,indicating the challenge of CKD in China.The major indicators of kidney damage include estimated glomerular filtration rate (eGFR),proteinuria and hematuria.It is recommended that laboratory should report eGFR when reporting results of blood creatinine.Both of Chinese equation and Chronic Kidney Disease Epidemiology Collaboration equation could be used.The process of creatinine measurement should be standardized.For evaluation of proteinuria,urinary creatinine should also be measured when using spot urine sample.Considering the high false positive rate and low false negative rate of urine routine test for hematuria,it is recommended that microscopic examination on urinary sediment should be done for those with positive hematuria by urine routine test.(Chin J Lab Med,2012,35:769-772)

Objective:To investigate the effects of soluble factors secreted by MCF-7 breast cancer cells on the differentiation,maturation and function of DCs.Methods:Mononuclear cells were cultured with the culture supernatants from primary MCF-7 cells.Combination of granulocyte-macrophage colony stimulating factor(GM-CSF),interleukin 4(IL-4) and tumor necrosis factor-a(TNF-a) was used to stimulate the cultured mononuclear cells.Then DCs and CTL assays were analyzed.Results:MCF-7 cell supernatant-treated DCs resulted in low expression of CD80,CD83,CD86 and HLA-DR,and the inhibition rate of CTL was 17.35% significantly lower than 56.14% induced by the control DCs(P＜0.01).IL-12 secreted by DCs and IFN-γ produced by PBMNC were all significantly lower than those in the control group (P＜0.01).Conclusion:Experiments in vitro shows that the culture supernatants from MCF-7 breast cancer cells could inhibit the development and functions of DCs.