Objective To explore the HO-1 expression levels with 5-FU chemosensitivity in esophageal squamous cell carcinoma.Methods Eca109 cells used in all experiments,MTT assay was used in cell growth curve and inhibit rate.RT-PCR and Western blot was used to detect HO-1 in cells treated with different concentrations of ZnppⅨ (0,20,80,100 μmol/L).Results The inhibitory rate of cells was significantly increased when the concentrition of ZnppⅨ increased.The inhibitory rate of cells in 80 μmol/L ZnppⅨ was higher than 20 μmol/L ZnppⅨ group(P＜0.05).The expression of HO-1 mRNA in each group was 0.50± 0.17,0.55±0.15,0.58 ± 0.09 and 0.55 ± 0.16,respectively,there was no significant difference between the two groups (P＞ 0.05).The expression of HO-1 was 0,85±0.07,0.63±0.11,0.43±0.12 and 0.25±0.10,respectively.The expression of HO-1 had significant difference (F=20.01,P＜0.01).Conclusion Eca109 cells inhibition rate positive correlated with ZnppⅨ concentrations,and ZnppⅨ were inhibited the expression of HO-1.not from gene level,but after the translation level.

Objective To analyze the effect and clinical value of health behavior intervention on postoperative hope level and negative emotional in patients with lung cancer surgery.Methods From December 2014 to October 2017,170 cases with lung cancer in Taizhou Hospital of Zhejiang were selected.According to the intervention methods,85 cases were divided into control group and observation group.The patients in the control group were treated with routine nursing,the observation group received routine nursing combined with healthy behavior intervention nursing mode,and the nursing effect of the two groups was analyzed.Results After intervention,the anxiety self-rating scale score,depression self-rating scale score in the observation group were(45.43 ±3.08)points,(46.02 ±3.45)points,respectively,which in the control group were(50.02 ±6.62)points,(53.65 ±6.31)points,respectively,and the differences between the two groups were statistically significant(t=5.287,5.187,all P<0.05).After intervention,the positive attitude,positive action,and keep close relationship with others in the observation group were(13.04 ±2.88)points,(12.99 ±2.97)points,(13.23 ±2.96)points,respectively,which in the control group after intervention were(10.02 ± 1.71)points,(10.13 ±1.86)points,(10.09 ±1.69)points,the differences between the two groups were statistically significant(t=4.871,5.017,4.418,all P<0.05).After intervention,the scores of physical function,role function,emotional function,cognitive function,social function,fatigue,nausea and vomiting,pain in the observation group were(74.04 ±7.73)points,(76.99 ±6.34)points,(75.01 ±7.86)points,(73.76 ±8.54)points,(75.92 ± 6.18)points,(77.98 ±7.08)points,(79.32 ±8.32)points,(72.11 ±7.18)points,respectively,which were higher than those in the control group,the differences were statistically significant(t=11.387,10.386,12.487,11.672,13.987,9.438,14.687,10.438,all P<0.05).Conclusion The application of healthy behavior intervention in patients with lung cancer can reduce anxiety and depression negative emotions,improve the hope level,improve the quality of life,and deserve to be applied in clinical application.