Objective To investigate the viral pathogens of children with community acquired pneumonia (CAP) in dalian city dur-ing the last year in hospital ,and provide evidences to clinical empirical therapy. Methods Some patients of department of respiratory, in Dalian children' s hospital were derided into four groups from May 2007 to 2008. Serum virus antibodies were detected after 1 week of nat-ural course of disease. Results In 280 cases who were detected for viral lgM antibodies, the positive rate was 28.2%. It was mainly con-sist with respiratory syncytial virus, adenovirus, and parainfluenza virus. The ratio of virus infection in school age group (54.0%) was higher than that in other groups. The second is in toddler age. Conclusion Virus are significant pathogens in CAP of children. The most common viral agent is respiratory syncytial virus (RSV), and the next one is adenovirus (ADV). The ratio of virus infection in school age group is the highest. We should give anti - virus and propotional supportive treatment to these children promptly.

Objective To investigate the viral pathogens of children with community acquired pneumonia(CAP) in dalian city during the last year in hospital,and provide evidences to clinical empirical therapy.Methods Some patients of department of respiratory.in Dalian children's hospital were devided into four groups from May 2007 to 2008.Serum virus antibodies were detected after 1 week of natural course of disease.Results In 280 cases who were detected for viral IgM antibodies,the positive rate was 28.2%.It was mainly consist with respiratory syncytial virus,adenovirus,and parainfluenza virus.The ratio of virus infection in school age group(54.0%) was higher than that in other groups.The second is in toddler age.Conclusion Virus are significant pathogens in CAP of children.The most common viral agent is respiratory syncytial virus(RSV),and the next one is adenovirus(ADV).The ratio of virus infection in school age group is the highest.We should give anti-virus and propotional supportive treatment to these children promptly.

Objective To explore the regulatory effect of apigenin on the blood pressure of the spontaneous hypertension rat(SHR) rats and the possible mechanism.Methods The case groups of rats were fed with different dosage of apigenin(0.03,0.05,0.11 g/kg) by gavage for 4 consecutive weeks,while the control groups were treated with equal cubage of oil.The body weight,heart rate,blood pressure were measured every week.Results After 4 weeks of treatment with apigenin,the blood pressure of SHR in all doses groups decreased significantly;besides,apigenin could reduce the content of ET-1 and AngII,however,it didn't change NOS activity.Conclusion Apigenin has a regulatory effect on blood pressure,its lowest effective dose is 0.03 g/kg bw and it may decrease the blood pressure through reducing the content of ET-1 and AngII,not through the NO/NOS pathway.

Long non-coding RNA urothelial carcinoma associated 1 (UCA1) is initially discovered and named in bladder cancer tissue,which is highly expressed in multi types of tumor tissues,such as bladder cancer,ovarian cancer,lung cancer,suggesting that UCA1 acts as oncogene.UCA1 is confirmed to regulate tumor cell proliferation,apoptosis,invasion and migration,which plays an important role in the occurrence and development of cancers.UCA1 is expected to become a new biomarker for diagnosis,prognosis and drug susceptibility,which may be a promising therapeutic target of cancer.

Objective To investigate the pharyngeal bacterial pathogens of children's community acquired pneumonia(CAP)in hospital,and provide evidences to clinical empirical therapy.Methods Three hundred and fifty-nine patients with CAP were selected.Before the treatment,pharyngeal swabs was taken for culture to find bacteria,then to be done drug sensitivity quantitative test and examed the extended spectrum β lactamages(ESBLs).Results The positive rate was 21.7%(78/359)and bacteria was 82 strains.It was mainly consisted with gram negative bacteria(73.2%,60/82),and klebsiella pneumonia (30.5%,25/82).Streptococcus pneumonia(18.3%,15/82)was the most common in gram positive bacteria.Klebsiella pneumonia was resistant to amoxicillin.Drug resistance of ESBLs producing strains was increased.Streptococcus pneumonia was highly resistant to erythromycin,clindamycin,and tetracycline.The ratio of bacteria infection in infancy age(34.6%.47/136)was higher than others.Conclusions Gram negative bacteria is significant pharyngeal bacterial pathogens in CAP of children.The most common ono is klebsiella pneumonia,and the corresponding one in gram positive bacteria is streptococcus pneumonia.Clinical doctors should avoid using high antibiotic-resistance drugs.

0.05 ); but rate of skin rash was increased in the patients with amoxicillin (P

Objective To investigate the pollution status of algae and microcystin(MC)in Dianshan Lake,Shanghai City and Poyang Lake,Jiangxi Province.Methods Water samples were collected from different sites of the above lakes,as the pretreatment of intracellular and extracellular microcystins was finished,MC-LR concentration was detected by ELISA.Results The proportion of Cyanobacteria in Dianshan Lake was 38.8% and 68.2% respectively,the mean content of MC was 281.78 and 1 145.77 pg/ml respectively.In Poyang Lake,the proportion of Cyanobacteria was 48.9% and 73.7% respectively,the mean content of MC was 80.53 and 405.65 pg/ml respectively.In the same water-blooming lake,MC content was significantly lower in July than that in October(P

Objective To investigate the efficacy of electromyographic biofeedback therapy (EMGBFT) in treating dysphagia after stroke.Methods Patients diagnosed with dysphagia after stroke were recruited and randomly divided into a control group (n =22),an electrostimulation group (ES,n =25) and an EMGBFT group (n =23).The control group received conventional treatment,while the patients in the other groups additionally received Vitalstim ES or EMGBFT 5 times per week for 3 weeks.Before and after the trial,deglutition function was evaluated through surface electromyography (sEMG) and using a standardized swallowing assessment (SSA).Results After 3 weeks of treatment,the mean sEMG amplitude,deglutition duration and SSA score and improved significantly in comparison to the baseline in all three groups.All were also significantly better in the two treatment groups than in the control group.Importantly,the mean sEMG amplitude,deglutition duration and SSA score were all significantly better in the EMGBFT group than in the ES group.Conclusion EMGBFT can promote better deglutition among patients with dysphagia after stroke more effectively than ES or conventional treatment.

Objective To study the gait footprint parameters of the normal children and the cerebral palsy (CP) children, and to explore its clinical value. Methods A total of 2 800 normal children aged 3 to 10 years and 139 spastic CP children aged 3 to 5 years were recruited in this study. The normal children were divided into seven groups with one year interval, and were measured with regard to the length of foot and step, step width and foot angle of footprint of every age group with self made oil printed carpet. The footprint of the CP children were measured and compared with that of the normal children at the same age. Results It was revealed that there was significant difference between the normal and the CP children ( P

OBJECTIVE:To establish a method for the simultaneous determination of 6 kinds of residual organic solvents in Omeprazole magnesium,such as methanol,isopropanol,acetonitrile,dichloromethane,ethyl acetate and toluenein. METHODS:Headspace GC was adopted. The determination was performed on the column of DB-624 column by temperature programming. The inlet temperature was 200 ℃,and hydrogen flame ionization detector was adopted with the temperature of 250 ℃;nitrogen gas was used as carrier gas with flow rate of 2.0 mL/min;the splitting-radio was 5 : 1,and sample size was 1 mL;the headspace tem-perature was 80 ℃ and the equilibrium time was 20 min. RESULTS:The linear range were 12.56-628.00 μg/mL for methanol(r=0.9997),20.22-1011.20 μg/mL for isopropanol(r=0.9999),1.96-97.76 μg/mL for acetonitrile(r=0.9997),3.10-154.88 μg/mL for dichloromethane(r=0.9998),20.69-1034.56 μg/mL for ethyl acetate(r=0.9998),and 3.53-176.72 μg/mL for toluene(r=0.9998);the limits of quantitation were 1.00,0.91,0.47,0.93,0.41 and 0.35 μg/mL respectively;the limits of detection were 0.31,0.30,0.14,0.31,0.12 and 0.11 μg/mL respectively;RSDs of precision,stability and reproducibility tests were lower than 3%;the recoveries were 94.53%-101.29%(RSD=2.15%,n=9),97.78%-103.42%(RSD=1.77%,n=9),96.99%-105.76%(RSD=2.59%,n=9),96.83%-102.05%(RSD=1.86%,n=9),97.98%-101.13%(RSD=0.88%,n=9)and 97.80%-102.40%(RSD=1.41%,n=9). CONCLUSIONS:The method is sensitive and accurate,can be used for the simultaneous determination of 6 kinds of residual organic solvents in Omeprazole magnesium,such as methanol,isopropanol,acetonitrile,dichloromethane,eth-yl acetate and toluenein.