Biosafety cabinets(BSC)is the primary containment barrier.It is very important to choose suitable control method for the protection ability of biosafety cabinet.This paper briefly introduces the key points and several control methods as well as the different characteristics of BSC control.

Objective To investigate the diagnosis and treatment of renal angiomyolipoma with hemorrhage. Methods Eighteen cases of renal angiomyolipoma with hemorrhage were diagnosed by imaging such as ultrasound and CT from June 2000 to December 2007.Open operations were adopted in 15 cases. Expectant treatment was adopted in 3 cases. Results Hemorrhage stopped in 3 cases with expectant treatment.All the operations succeeded.Pathological diagnosis after operations was renal angiomyolipoma.The follow-up time was 3 to 60 months.Ultrasound,CT and intravenous pyelography were mainly methods.There was no case of relapse or fistula.Tumor size of 3 cases with expectant treatment changed little. Conclusions Diagnosis of renal angiomyolipoma with hemorrhage should be based on clinic and imaging.CT and ultrasound have better diagnosis effect.Expectant treatment,selective embolization of renal artery,tumor excision and heminephrectomy are main methods.Nephrectomy can also be adopted when there is serious hemorrhage.

Objective Direct impact is a common mechanism of injury for blunt thoracic injury, and if resulting thoracic injury is severe the mortality may be as high as 10% ～ 25% . We aim to develop a mechanical device for animal impact injury experiment, so as to establish a rodent model of severe thoracic injury. Method A spring operated mechanical device for animal impact injury experiment was developed. The device allowed for accurate controlled delivery of impact force to specific areas of the chest well, at specific velocities and degrees of chest compression. Eghty-four male Sprague-Dawley rats were anaesthetized and underwent left carotid artery cannulation. They were randomly divided into seven groups and given the following treatment: group A (Control group) were subjected to sham impact; group B to G animals were subjected to impacts on the right lateral superior chest at different velocities and degrees of chest wall compression. ( B 3 m/s, 20%; C 3 m/s 40%; D 6 m/s 20%; E 6 m/s 40%; F 9 m/s 20%; G 9 m/s 40%). Arterial blood gas samples were taken just before injury, and at 2 and 12 post injury. All rats were sacrificed at 12 hours and their degree of thoracic injury rated. Pathological examination of injured lung tissue was also performed. Results The device was able to deliver impact forces accurately, with ＜ 4% deviation from desired velocity and ＜ 3 mm deviation from target area of impact. Other than the control group, all animals experienced significant hemodynamic changes immediately post impact. Arterial blood gas analysis detected significant hypocapnia in groups B and C. Significant hypoxemia and hypocapnia was detected in groups D, E and F. In groups B,C,D and F, die impact produced a mild thoracic injury with low mortality rate at 12 hours. In group E, the impact produced severe thoracic injury with mortality rate of 33.33% at 12 hours. Group C animals sustained the most serious thoracic injury with mortality rate of 83.33% at 12 hours. Pathological examination revealed injuries from direct trauma as well as secondary lung injuries. Conclusions Our device was able to repetitively deliver accurate and precise impact forces to rats and allows us to establish a rodent model of severe thoracic injury firm blunt trauma. We found that with our device, impact force at velocity of 6 m/s and 40% chest compression produced the most severe lung injury in rats.This helps us establish a rodent model of severe thoracic injury which can be use for future research in severe blunt thoracic trauma and the secondary lung injuries.

Objective:To study the inhibitory role of BsAb(anti-human bladder cancer/anti-VEGF) on human bladder cancer growth.Methods:BsAb was used to treat animal models of BIU-87 cell lines.The tumor inhibition rate was calculated and the tumor microvessel density was detected by immunohistochemical techniques.Results:BsAb could significantly inhibit growth of tumor.And the tumor microvessel density was lower than that of the control group.Conclusion:BsAb could inhibit tumor growth by inhibiting angiogenesis. BsAb may become a new method for human bladder cancer treatment.

Purpose:To study the relationship between expression of TGF-?_(1) and invasion and metastasis of bladder cancer.Methods:ELISA was used to examine TGF-?_(1) in serum of patients with bladder cancer.Results:Compared to the control group,TGF-?_(1) level in patients serum was higher(P

Objective To investigate the efficacy of transurethral electroresection combined with intravesical irrigation with epirubicin for cystitis glandularis.Methods From January 2000 to January 2006,130 patients with cystitis glandularis were treated with transurethral electroresection.The whole depth of diseased mucosa and adjacent normal mucosal tissues(1 cm away)were resected.One week after the operation,intravesical irrigation with epirubicin were carried out(50 mg per time,once a week for 8 weeks,and then once a month for 2 years).Results Five of the patients developed urethral stenosis after the electroresection,and were cured by dilating the urethra at a regular interval.No complications occurred in the other patients.Bladder symptoms disappeared in 98 patients;23 cases had urinary symptoms occasionally,but cystoscopy showed normal vesical mucosa.Nine of the patients had recurrent cystitis,5 of which showed non-irrigation-induced hematuria 6-12 months after the operation,and 4 were found during regular re-examination by cystoscopy.Transurethral electroresection was performed again on these 9 patients,after they were diagnosed by biopsy;and then intravesical irrigation with hydroxycamptothecin(20 mg per time)were given.They were followed up for 12 months,during which no one had recurrence.Conclusions Transurethral electroresection combined with intravesical chemotherapy is effective for cystitis glandularis.The method is worth being widely used.

Objective To investigate the efficacy of Holmium laser for upper urinary tract diseases.Methods A total of 101 patients with urinary tract stricture or calculus in the lower or middle ureter were treated with holmium laser by ureteroscopy from January 2005 to June 2007 in our hospital.For the patients with stones in the upper urinary tract or the kidney,percutaneous nephrolithotripsy(PNL) with holmium laser by ureteroscopy was performed.Results No patients developed perforation,urinary leakage,or infection after the operation.In the 23 cases of ureteral stricture,6 were cured,10 were improved,5 were failed,and 2 were deteriorated or even developed hydronephrosis.The two who were deteriorated after the operation was converted to open surgery because of pain in the kidney area and refractory infection.They were cured after resection of the strictured segment and end-to-end anastomosis.Follow-up was available for 3 to 24 months(mean,12 months);none of the patients had recurrence during the period.In the 40 patients with lower or middle ureteral stones,nephrolithotripsy was completed successfully in all but 7,in whom the stones moved into the kidney during the procedure.These 7 patients were then cured by extracorporeal shock wave lithotripsy.The 40 patients were followed up for 3 to 18 months(mean,15),none of them had recurrence.In the 24 cases of upper urinary tract stones and 14 patients with renal calculus,the stones were removed completely by the first PNL in 36;2 patients received a second operation after indwelling renal cannula for 1 week because of intraoperative hemorrhage.These 38 patients achieved a follow-up of 3 to 24 months(mean,18),no one of them showed residual stones,or recurrent calculus or stricture of the ureter.Conclusions Holmium laser by ureteroscopy is safe,effective,and minimally invasive for patients with upper urinary tract disorders.The treatment is worth being used thanks to a low rate of intra-and postoperative complications.

Objective To discuss the clinical efficacy of transurethral electroresection in the treatment of ureteral orifice tumors. Methods A total of 65 cases of ureteral orifice tumor were treated with transurethral electroresection and chemotherapy.Results The operation time was 5~90 min(mean,35 min),and the intraoperative blood loss was 5~100 ml(mean,50 ml).No blood transfusion was required.No fatal case was encountered.Out of the 65 cases,there were 53 cases of transitional cell carcinoma(grade Ⅰ,43 cases;grade Ⅱ,10 cases) and 12 cases of papilloma.The 65 cases were followed for 6~24 months(mean,15 months).Postoperative urethral stricture happened in 4 cases,which were cured by dilation of the urethra.Recurrence of tumor at the trigone and lateral wall of the bladder occurred in 5 cases,which were given a re-operation of transurethral electroresection and chemotherapy and were followed for 10~20 months(mean,15 months) without recurrence.Conclusions Transurethral electroresection of ureteral orifice tumors gives satisfactory effects and should be further recommended.

Objective To find a new method for quality control of polysaccharides by establishing the fingerprint profiles of polysaccharides extracted from Guifu Dihuang Wan. Methods The polysaccharides extracted from Guifu Dihuang Wan were hydro-lyzed partially under appropriate hydrolysis condition with acid,and the fingerprint profiles of the hydrolyzates was obtained by HPLC. Microsoft Excel 2002 was applied for calculating the similarity of fingerprint profiles,and informatton analysis of these profiles was performed by a software for evaluating fingerprint profiles of traditional Chinese medicine. Results Fingerprint profiles of the hydroly-zates of polysaccharides from 10 of Guifu Dihuang Wan products,produced by different manufacturers,were obtained. It was observed that the similarity of eight products was greater than 0.99,while the similarity of the other two was 0.92 and 0.94,respectively. Con-clusion This method is technically feasible for the quality control of polysaccharides from Guifu Dihuang Wan.

Objective To discuss the efficacy of transurethral electro-resection combined with needle electrode treatment for bladder neck contracture after treatments of benign prostatic hyperplasia(BPH).Methods A total of 45 cases of bladder neck contracture that developed after treatments of BPH were treated with transurethral electro-resection combined with needle electrode treatment in our hospital.During the operation,the raised posterior lip of the bladder neck was cut at 6 o’clock point,and then the scar tissues at 3 and 9 o’clock points were resected to expose the bladder detrusor.The scar tissues at 12 o’clock point should be removed as much as possible so that the bladder neck could be repaired easily.Afterwards,the fibrotic ring of the bladder neck was cut through at 5 and 7 o’clock points with a needle electrode,the fat tissues covering the bladder could be exposed.Results After the operation,43 of the cases restored normal urine flow.The other 2 patients could urinate despite of mild dysuria,and the symptom was then improved by oral ?1 antagonist and M agonist.Fifteen patients developed micturition frequency and urgency,and odynuria,and were cured pontaneously in two weeks.No patient showed urinary bleeding,stricture,incontinence,or recto-vesical fistula.Follow-up was available in the patients for 3 to 30 months(mean,15 months),during the period,reexaminations showed a Qmax of 15.5-24.3 ml/s(mean,19.5),and residual urine after urination of 0-35 ml(mean,20).No relapse of bladder neck contracture occurred in this series.Conclusions Transurethral electro-resection combined with needle electrode treatment is effective for patients with bladder neck contracture after treatments of BPH.The rate of relapse is low after the operation.