Tissue expression and blood level of YKL-40 protein are elevated ia patients with tumor.The protein may have a role in cancer cell proliferation,infiltration,metastasis and remodelling of tumor stroma.Elevated YKL-40 levels are highly associated with short survival and poor response to treatment for patients.Thus,YKL-40 may serve as a biomarker in diagnosis of disease,evaluation of severity and assessment of prognosis.It's more likely to be a new target for anti-tumor treatment.

Objective To evaluate the lithotripsy efficiency of a newly calculi anchoring forceps in vitro.Methods Compared the efficiency with or without calculi anchoring forceps in the circumstances imitate percutaneous nephrolithotomy.There are 24 calculus for each group in random selection.The lithotripsy was completed by one people.the time,energy and retained calculus were compared by T-test.Results When the energy expenditure reaches 2 kJ,the operating time was (4.7 ± 0.9) min in group 1 and (4.2 ± 0.9) min in group 2 (P ＞ 0.05).The removal calculus showed no differences between the two groups (P ＞ 0.05).After the energy used over 2 k J,the operating time of the remained calculus were (4.5 ± 1.2) min in group 1 and (9.2 ± 2.6) min in group 2 respectively (P ＜ 0.05),and the energy consumed (1.7 ± 0.5) kJ and (2.6 ± 0.4) kJ respectively (P ＜ 0.05).Total energy consumed in group 1 and group2 was (3.7±0.5) kJ and (4.6±0.5) kJ (P＜0.05).And the cumulate time used was (9.2±1.7) min and (13.4 ±2.7) min in group 1 and group 2 respectively (P ＜0.05).Conclusions The calculi anchoring forceps can improve the efficiency of lithotripsy in vitro.The improvement was significant for the calculus whose diameter was smaller than lcm.

Objective To analyze the causes of poor compliance of elderly patients with diabetes mellitus , and put forward corresponding countermeasures to control blood glucose level and delay the occurrence of complications .Methods A total of 113 elderly patients with type 2 diabetes mellitus aged 60 years and over were recruited in endocrinology department of our hospital.The self-made questionnaire was used to investigate the compliance and the factors that affected the compliance.Results There were 48 patients (42.48%) with good compliance and 65 patients (57.52%) with poor compliance.Univariate analysis showed that the level of education, monthly income, drug types, understanding of diabetes-related knowledge and whether the elderly living alone affected compliance ( P<0.05 ) .Multivariate Logistic regression analysis showed that patients with low education level, poor monthly income, medication types and living alone were the main factors that influence the compliance of elderly patients with diabetes mellitus (P<0.05).And the level of education, monthly income level and the compliance of diabetic medication was positively correlated, medication types and living alone and diabetes compliance were negatively correlated (P<0.05).Conclusion The low level of education, poor monthly income, medication types and living alone are the main factors that affect the compliance of elderly patients with diabetes.The social and family support can effectively improve the compliance of elder patients with diabetes.

Acute kidney injury(AKI) is a clinical critical syndrome caused by a variety reason,sepsis is the leading cause and independently associated with mortality in critical patients.Fluid resuscitation is one of the most important treatment of spesis and AKI.Fluid overload has been shown to be associated with worse outcomes in critically ill patients.Different liquid treatment should be adopted in different stages.To carry out dynamic,noninvasive hemodynamic monitoring is the best way to critical patients with AKI liquid management.

Objective Through the DNA barcoding 12s rRNA sequences alignment and analysis of several rhinoceros horns products involved in cases to analysis the application feasibility of 12s rRNA in the rhinoceros horns products’ species identification. Methods Use rhinoceros horns products in 3 cases as materials, total DNA were extracted with improved method, PCR ampliifcation the DNA barcoding. Results The alignment and analysis of sequences show that 12s rRNA could identify rhinoceros horns products at the species level. Conclusion The DNA barcoding 12s rRNA could be used as a new way to identify the rhinoceros horns products which can’t be identiifed with morphological characteristics, provide a reliable basis for the qualitative and sentencing of cases.

BACKGROUND:Bone marrow mesenchymal stem cel s can migrate into tumor tissues, as reported in recent studies.
OBJECTIVE:To investigate the tropism and effect of transplantation of bone marrow mesenchymal stem cel s on proliferation and differentiation of gastric cancer cel line SGC7901 in rats.
METHODS:Gastric cancer models were established in Sprague-Dawley rats by subcutaneous injection of gastric cancer cel line SGC7901, and then, in cel transplantation group, each rat underwent an intraperitoneal injection of 1×107 bone marrow mesenchymal stem cel s. After transplantation, the targeting ability of bone marrow mesenchymal stem cel s was detected using fluorescent DiI labeling. Cyclin D2 mRNA and protein expressions were measured using real-time PCR and western blot assay, respectively. Apoptosis of gastric cancer cel s was observed by in situ terminal labeling method.
RESULTS AND METHODS:Bone marrow mesenchymal stem cel s successful y migrated into the gastric cancer site in rats. The expression levels of cyclin D2 mRNA and protein in the cel transplantation group were significantly higher than those in the model group (P<0.05). It was observed that the number of apoptotic cancer cel s was significantly reduced in the cel transplantation group compared with the model group (P<0.05). These findings indicate that bone marrow mesenchymal stem cel s have the ability to migrate into the tumor sites, thereby promoting the proliferation of gastric cancer cel s.

目的探讨实施社保医疗后肺部感染患者的治疗效果与费用。方法随机选择肺部感染的住院患者60例(社保组30例,非社保组30例),社保组根据社保文件精神对患者进行检查治疗,非社保组仍按以往治疗方案并结合患者意愿进行检查治疗,分析两组的疗效与费用。结果社保组的肺部感染住院患者总的直接医疗费用平均为(1540.73±991.43)元,非社保组为 (2232.02±844.98)元;总医疗费用社保组平均为(1716.65±1028.33)元,非社保组为(2653.89±966.39)元;住院天数社保组平均为(11.8±6.42)天,非社保组为(15.3±4.71)天 ,社保组总的医疗费用显著低于对照组(P<0.001),但疗效无显著性差异。结论实施社保医疗可在有效降低肺部感染患者总的医疗费用的同时,保证疗效,真正把有限的医疗资源用在需要的患者身上。

Objective Direct impact is a common mechanism of injury for blunt thoracic injury, and if resulting thoracic injury is severe the mortality may be as high as 10% ～ 25% . We aim to develop a mechanical device for animal impact injury experiment, so as to establish a rodent model of severe thoracic injury. Method A spring operated mechanical device for animal impact injury experiment was developed. The device allowed for accurate controlled delivery of impact force to specific areas of the chest well, at specific velocities and degrees of chest compression. Eghty-four male Sprague-Dawley rats were anaesthetized and underwent left carotid artery cannulation. They were randomly divided into seven groups and given the following treatment: group A (Control group) were subjected to sham impact; group B to G animals were subjected to impacts on the right lateral superior chest at different velocities and degrees of chest wall compression. ( B 3 m/s, 20%; C 3 m/s 40%; D 6 m/s 20%; E 6 m/s 40%; F 9 m/s 20%; G 9 m/s 40%). Arterial blood gas samples were taken just before injury, and at 2 and 12 post injury. All rats were sacrificed at 12 hours and their degree of thoracic injury rated. Pathological examination of injured lung tissue was also performed. Results The device was able to deliver impact forces accurately, with ＜ 4% deviation from desired velocity and ＜ 3 mm deviation from target area of impact. Other than the control group, all animals experienced significant hemodynamic changes immediately post impact. Arterial blood gas analysis detected significant hypocapnia in groups B and C. Significant hypoxemia and hypocapnia was detected in groups D, E and F. In groups B,C,D and F, die impact produced a mild thoracic injury with low mortality rate at 12 hours. In group E, the impact produced severe thoracic injury with mortality rate of 33.33% at 12 hours. Group C animals sustained the most serious thoracic injury with mortality rate of 83.33% at 12 hours. Pathological examination revealed injuries from direct trauma as well as secondary lung injuries. Conclusions Our device was able to repetitively deliver accurate and precise impact forces to rats and allows us to establish a rodent model of severe thoracic injury firm blunt trauma. We found that with our device, impact force at velocity of 6 m/s and 40% chest compression produced the most severe lung injury in rats.This helps us establish a rodent model of severe thoracic injury which can be use for future research in severe blunt thoracic trauma and the secondary lung injuries.

Objective To study the value of plasma level of matrix metalloproteinase-9 (MMP-9) for the early diagno-sis of Kawasaki disease. Methods The difference in MMP-9 level was studied by retrospective nested case-control method between children with early Kawasaki disease, Henoch-Sch?nlein purpura (HSP) or respiratory infection, and healthy control children. The associations of MMP-9 with serum procalcitonin, erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were also analyzed. The cutoff-value, sensitivity and speciifcity of MMP-9, ESR and CRP in diagnosis of Kawasaki disease were studied by ROC curve. The areas under ROC curves were compared among different diagnostic markers to deter-mine their signiifcances in diagnosis of Kawasaki disease. Results The plasma level of MMP-9 was increased in early phase of Kawasaki disease, and higher than that in children with HSP or respiratory infections and in healthy controls (P<0.05). If MMP-9, ESR and CRP cutoff value were set to be 90.23 ng/ml, 56.5 mm/h and 27.55 mg/L, the sensitivity, speciifcity and area under ROC curve was 83.3%, 86.4%and 0.904, 95.8%, 66.1%and 0.807, 83.3%, 74.6%and 0.789 respectively. The diagnostic performance of MMP-9 for Kawasaki disease was better than that of ESR and CRP. Conclusions The plasma level of MMP-9 is increased in the early stage of Kawasaki disease. The sensitivity and speciifcity of MMP-9 in diagnosis of Kawasaki disease are highest if cutoff value is set to be 90.23 ng/ml.

AIM: To explore the relationship between the alteration in gene expression of sarcoplasmic reticulum Ca~(2+)-ATPase (SERCA) and phospholamban (PLB) in spontaneously hypertensive rats (SHR). METHODS: 294 samples of total RNA were obtained from the tissue of ventriculum , aortic smooth muscle, liver and kidney in SHR and normotensive rats (WKY). RNA array was used to determine the mRNA levels of SERCA and PLB. RESULTS: Compared with age-matched WKY rats, the systolic blood pressure increased higher in 6-week-old SHR (P