Objective:To study histocompatibility of self-made stents made of NiTi alloy in rabbits.Methods: To place 30 stents made of NiTi alloy into 30 rabbits trachea through mouth under general anethesia,to observe local tissue reaction from 1 week to 6 months and to try to find pathological changes of main organs of rabbits in the 6 th month. Results:During observation,apart from dyspnea in two rabbits,one died at 3 day and the other died at 6 week after implantation. Autopsy showed the implanta.tion sites were occluded by granulation tissue. All other rabbits were normal and implantation sites were patent. In the first three weeks we could observe acute inflammation at the implantation sites while after 5 weeks we could observe chronic inflammation there. We observed that the structure of epithelial cells tended to be normal by telemicroscope. There are no pathological changes in liver, spleen, kidney, lung, heart and brain tissue. Conclusion:Self-expanding stents possess good histocompatibility and have no toxic reaction to whole body and fit to implantation into human body.

OBJECTIVE:To investigate clinical efficacy and safety of trastuzumab combined with neoadjuvant chemotherapy on breast cancer,and discuss its effects on the expression of HER2 protein,B7 homolog 1(B7-H1)and IL-2. METHODS:62 patients with breast cancer confirmed by pathological biopsy,whose test results of immunohistochemistry combined with fluorescence in situ hybridization(FISH)was overexpression of HER2,were selected as the research object. They were randomly divided into observa-tion group and control group with 31 cases in each group. Control group was given carboplatin combined with docetaxel neoadjuvant chemotherapy before surgery. Observation group was additionally given Trastuzumab for injection intravenously 4 mg/kg in the first week,2 mg/kg in second-eighteenth week,once a week,for consecutive 18 weeks. Modified radical mastectomy was conducted 2 weeks after chemotherapy. Clinical efficacy and the occurrence of ADR were observed in 2 groups. The expression of HER2 protein, B7-H1 and IL-2 in tumor tissues were compared between 2 groups before and after treatment. With 1-year follow-up,tumor recur-rence rates,metastasis rates and survival rates were recorded in 2 groups. RESULTS:After treatment,total effective rate of observa-tion group was 83.87%,which was significantly higher than that of control group (58.06%),with statistical significance (P<0.05);the test result of HER2 protein in lesion tissues was non-overexpression in 19 cases (61.29%) in observation group which was significantly higher than that of control group(11 cases,35.48%),with statistical significance(P<0.05);the positive expres-sion rates of B7-H1 and IL-2 in lesion tissues of observation group were significantly lower than those before treatment and control group after treatment,with statistical significance(P<0.05). There was no statistical significance in the incidence of ADR between 2 groups (P>0.05). During 1-year follow-up period,except that there was no statistically significant difference in survival rate be-tween 2 groups(P>0.05),the recurrence rate and metastasis rate of observation group were significantly lower than those of control group,with statistical significance(P<0.05). CONCLUSIONS:The application of trastuzumab combined with neoadjuvant chemo-therapy before breast cancer modified radical mastectomy can effectively improve the expression levels of HER2 protein,B7-H1 and IL-2 in lesion tissue of patients with breast cancer,promote clinical efficacy with good safety and low risk of tumor recurrence and metastasis after operation.

Du meridian is one of the eight extra meridians, having the functions of defending pathogenic factors,inducing diaphoresis to dispel cold, warming the viscera and bowels, elevating the pure yang, promoting the flow qi by warming channels, promoting blood circulation to stop pain, clearing heat by dredging du meridian, treating carbuncle and malaria, dredging du meridian to treat encephalic disease, nourishing brain and nourish marrow, benefiting intelligence and spirit, waking up patient from unconsciousness, relieving convulsion, calming the nerves, stopping endogenous wind, calming the spirit. Moreover it also has the functions of sectional treatment and antidotal health care. Du meridian is widely used clinically in treating many kinds of diseases.

Objective To investigate main causes leading to conversions to open surgery during laparoscopic cholecystectomy (LC) in the treatment of acute cholecystitis. Methods A total of 32 cases of acute cholecystitis received LC were analyzed. Results Out of the 32 cases of acute cholecystitis, LC was successfully accomplished in 25 cases (78.1%) and a conversion to open surgery was required in 7 cases (21.9%). Major causes of the conversion included the necrosis of the gallbladder(4 cases) and the failure to expose the Calot’s triangle(3 cases). Conclusions Conversions to open surgery should be made during LC when a gangrenous cholecystitis is confirmed or the Calot’s triangle cannot be well exposed.

Objective To study the effect of nonylphenol (NP) on the fertility of mice,and assess its reproductive and developmental toxicity.Methods 128 mature KM mice weighed 22-26 g were randomly divided into three experimental groups (24,60 and 120 mg nonylphenol/kg bw respectively) and the control group (earthnut oil ),16 in each.Fertility tests were performed according to the procedure commended by EPA.Results Sperm number,sperm vitality,fertility index and pregnancy rate decreased,while rate of deformed sperm increased in dose-dependent manner at dose of 60 and 120 mg/kg bw (P

0.05).The results of Western blotting and immunohistochemical method showed that HO-1 protein expression increased in DOX group and DOX-IR group,and was negative in normal group.More distruction of ultrastructural changes of hepatic cells in IR group was found than in DOX-IR group by transmission electron microscope.Conclusion Doxorubicin pretreatment could protect the liver from ischemia-reperfusion injury,which may be related to HO-1 expression induced by doxorubicin,low dosage of doxorubicin does little harm to rat liver.

Objective To construct the RNAi vector targeting survivin gene, in order to observe its effect on lung adenoearcinoma A549 cell radiosensitivity, and explore the possible mechanism. Methods One pair of RNA interference sequence targeting survivin gene was designed according to the eDNA sequence, the recombinant RNAi plasmid pGenesil2-survivin was constructed. After certified by enzyme digestion and sequencing, the pGenesil2-survivin plasmid was transfected into A549 cells. Survivin mRNA and protein was measured by RT-PCR and Western blot, respectively. The apoptosis was analyzed with flow cytometry. The radiosensitivity was measured by clone formation assay. Results The pGenesil2-survivin vector was successfully established. After the pGenesil2-survivin was transfeeted into A549 cells for 48 h, surviving protein and mRNA in pGenesil2 group were not obviously changed compared with that in normal group. They were increased after 5 Gy X-ray irradiation, but obviously inhibited in pGenesil2-survivin group. The cell apoptosis in pGenesil2-survivin and 5 Gy X-ray groups was obviously increased (t1 = 10.63, P < 0.001 ; t2 = 3.75 , P < 0.05), the effect was more manifest in combined group(t = 4.83, P < 0.05). D0 and Dq in normal and pC, enesil2 group were not obviously different, but those in pGenesil2-survivin group were lower. Conclusions RNAi targeting survivin gene could inhibit survivin mRNA and protein expression, enhance the A549 cell apoptosis rate and cell radiosensitivity.

ObjectiveTo investigate the psychological state and distribution of emotional symptoms of women during peri-menopause.Methods56 women with some emotional disorder (sick group) and 37 women without emotional symptom (control group) peri-menopause were measured with Symptom Checklist 90 (SCL-90).ResultsThe scores of total, quantity of positive items,average of positive,somatic factor,compulsion,depression and anxiety,phobia in sick group were more than that of control group significantly.ConclusionWomen during peri-menopause present serious emotional disorder mainly in somatization, compulsion,depression, anxiety and phobia.

Objective To evaluate the diagnostic value of tracking movement of normal patellar using volume scan on sensation 320 CT. Method Data of dynamic scans of 30 knees was collected using the motor function of 320 CT and retrospectively analyzed. The data of movement of the patellorfemoral joint was obtained during flexion (from 0° to 120°) within 10-sec by 320 CT from all volunteers. The 3D coordinate of the center of patella was recorded to investigate the dispose relation of patellofemoral joint.Result With the knee angle changed from 0° to 90°, the patella moved rapidly along the Y-axis direction ( sagittal plane) down about (53.87 ± 0. 45 ) mm, and then entered the plateau phase with little change.When the knee flexion reached 10°-30° ,the patellar movement along the X-axis reached the largest range of (2. 31 ±0. 52)-(3.36 ± 0. 43 ) mm, and subsequently moved to the opposite lateral direction with the maximum about (8. 53 ± 0. 44 ) mm at 120°. In the Z axis, the track initially showed plateau, and then presented a rapidly downward trend after 30°. The patellar tracking is like an outward arc during the whole knee flexion. Conclusion The motor functional imaging of 320 CT can pinpoint the patelar tracking in a fast, painless way.

Objective To study the function of Contour stapler in the sphincter-preserving surgery in 121 cases with low rectal cancer. Methods One hundred and twenty-one cases enrolled were divided into 2 groups randomly and accepted Contour or other kinds of staplers after operation respectively. Further comparison were performed on the rate of sphincter-preserving,anastomosis stricture and leakage,infection of incision and stool frequency between two groups. Results We found no significant differences in the comparison of rate of anastomosis stricture or leakage,stool frequency,but the Contour group had significant higher rate of sphincter-preserving (98. 3%)than other stapler group(68. 9%),and lower rate of infection of incision(3.94%)than control(11.9%)(P ＜0. 05 respectively). Conclusions It is secure,reliable and practical to use Contour stapler in operation on low rectal cancer,which can increase the opportunity of sphincter-preserving,decrease the the rate of infection of incision.