Objective To investigate the influencing factors of medication compliance of glucocorticoid for patients with interstitial lung disease (ILD). Method The outline in-depth interviews with 15 patients with ILD was done using content analysis method, and the influence factors of patients medication compliance were analyzed. Result The influence factors of medication compliance in ILD patients can be summarized as the following three themes: patients′individual factors, factors related to medical and absence of social and family support. Conclusion Nurses should master the influencing factors of hormone medication adherence in ILD patients, ready to take appropriate intervention measures, improve the medication compliance in patients with ILD hormone and promote their rehabilitation.

Objective　To investigate the influencing factors of rifampicin resistance and the mutation frequency and distribution of rpoB gene in Mycobacterium tuberculosis, and to analyze the mutation characteristics of resistance-determining region (RRDR), so as to provide reliable laboratory evidence for the epidemic trend, prevention, and treatment of rifampicin-resistant tuberculosis. Methods　A total of 312 patients with pulmonary tuberculosis who were treated in the Second People's Hospital of Fuyang from March 2019 to December 2021 were selected. After Xpert MTB/RIF and liquid drug susceptibility test, 156 patients with rifampicin-resistant pulmonary tuberculosis were selected as the rifampicin-resistant group, and 156 patients with non-drug-resistant pulmonary tuberculosis were selected as the general group. The general information and rpoB gene mutation of the two groups of patients were analyzed. Results　Among the 312 cases of pulmonary tuberculosis patients, the presence of complications, retreatment, self-medication, non-standard medication, treatment interruption, and low awareness of transmission routes were significantly higher in the rifampicin-resistant group than in the general group, and the differences were all statistically significant; logistic regression analysis showed that comorbidity and retreatment were the risk factors of rifampicin resistance. Among the 156 rifampicin-resistant specimens, 152 (97.44%) had single-gene mutations and 4 (2.56%) had double-gene mutations. The highest single-gene mutation was ProbeE, followed by ProbeD; the double-gene mutations were D+E, followed by A+B; statistically significant differences were observed for ProbeE between the initial treatment and retreatment groups (χ2=3.97, P<0.05), while others showed no statistically significant differences; among 118 multidrug resistance mutations, ProbeE accounted for the highest proportion, followed by ProbeD; there were 38 cases of single rifampicin resistance, and the proportion of mutation sites from high to low was ProbeE and ProbeD; all 4 cases of double-gene mutations were MDR-TB; there were no statistically significant differences in mutation sites between MDR-TB and RR-TB. 　Conclusions Attention should be paid to pulmonary tuberculosis patients with comorbidities and retreatment patients, and timely intervention should be given to reduce the probability of rifampicin resistance. The rpoB gene mutations in rifampicin-resistant patients are mainly single-probe mutations, with no difference in the rpoB gene mutations sites between the rifampicin-resistant patients and multidrug-resistant patients. Keywords: Mycobacterium tuberculosis; rifampin; gene; mutation

Objective To observe the effects of grape seed proanthocyanidin extract(GSPE) plus atorvastatin treatment on matrix metalloproteinase-9 ( MMP-9) in experimental atherosclerosis rabbits and to explore its mechanism. Methods Thirty male New Zealand rabbits were randomized into five groups. The normal control group were fed with standard diet for 24 weeks. And the other groups were fed with standard diet containing 1 % cholesterol for 12 weeks. In the sequential 12 weeks, the model control group was fed with standard diet. The GSPE group was fed with standard diet containing 1% grape seed proanthocyanidin(GSP). The atorvastatin group was fed with standard diet containing atorvastatin(2. 5 mg · kg~(-1) · d~(-1)). The GSPE plus atorvastatin group was fed with standard diet containing 1% GSP and atorvastatin (2. 5 mg~(-1) · kg~(-1) · d~(-1)). Blood samples were drawn from ear middle arteries of rabbits just before the experiment and at the 12th and the 24th weekend of the experiment. All the rabbits were fasted for at least eight hours before the blood was drawn. The blood samples were analyzed for the matrix metalloproteinase-9 (MMP-9). All the rabbits were sacrificed at the 24th weekend, and the expression of MMP-9 was observed in the thoracic aortic tissue using immunohistochemistry technique. Results The serum level and aorta expression of MMP-9 were increased in model group compared to control group (all P< 0. 05). The severity of atherosclerosis was less in three drug groups than that in model control group. The GSPE,atorvastatin and GSPE plus atorvastatin groups versus model group showed less atherosclerotic lession, the decreased expression of aorta MMP-9 and the decreased serum level of MMP-9 C(1. 06±0. 21), ( 1. 07 ±0.20), (0.81 + 0.16) vs. (1. 32±0. 24)ng/ml,all P<0. 05]. The effect in the GSPE and atorvastatin group was most obvious. Conclusions GSPE plus atorvastatin group has the most efficacy of anti-atherogenesis, which is associated with its reducing the expression of matrix metalloproteinase.

AIM protective effects of the Marine Face Pack (MFP) on hairless mice damaged by ultraviolet B(UVB) and it's relation to antioxidants were studied. MATHODS oxidative damage models of hairless mice irradiated by ultraviolet B (radiating intension; UVB 5. 15 ? 10-2J/cm2 ? 30 day) were established. The mice were randomly divided into four groups: control, UVB radiated groups (Water model, 10%MFP, 10%VitC). The ultrastructures of skin were observed by electromicroscope. The expression of Bcl - 2 protein and NOS were examined by immunohistochemical methods. The contents of MDA, the activities of GSH-Px, SOD and the T-AOC in the supernates of skin of those mice were detected by biochemical methods. RESULTS The large dilatant vacuolations and few rough endoplasmic reticu-lum in fibroblasts and epidermic cells of mice in UVB model group were observed under electromicroscope. MFP could keep the ultrastructure of epidermis and fibroblasts in the mice of MFP groups normal. MFP could enhance the expression of Bcl-2 protein and inhibit the ac-tivitiy of NOS(P

Objective To evaluate the value of fat-saturated three-dimensional fast imaging with steady state procession(FS-3D-FISP)sequence in detecting hyaline cartilage defects in osteoarthritis in rabbits.Methods Osteoarthritis was induced in eighteen male rabbits by injecting papain into the left knee joints.The same volume of sterile saline solution was injected into right knee joints as controls.MRI of knee joints was done at 1,2 and 4 weeks after injection using FS-3D-FISP sequence.The the rabbits were killed and pathohistological examinations of the femoral condylars and tibial plateaus were performed.The cartilage changes were observed by MR image according to the Outerbridge method and by pathology using Collins staging.Results There was closely correlation between MR imaging and pathology in grading of cartilage injury in grade I～II and grade III～IV(P＜0.001).Conclusion FS-3D-FISP can evaluate accurately the degree of articular injury.

Objective To explore the relationship between morphological features of carotid arterial atherosclerosis plaque and various cardiovascular risk factors in the aged.Methods The 321patients who underwent cerebral angiography were classified into two groups:256 patients with carotid arterial atherosclerosis and 65 patients without plaque.The relationships between carotid arterial plaque and cardiovascular risk factors including age,gender,cholesterol(TC),triglyceride (TG),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol(LDL-C),C-reactive protein(CRP),homocysteine (HCY),hypertension,diabetes,smoking and drinking were analyzed.The relationships between morphological features,including the stenosis degree,surface fairing of plaque,and cardiovascular risk factors were analyzed.After 1 year follow-up,the prospective cardiovascular and cerebrovascular events were analyzed in the two groups.Results Carotid atherosclerosis was connected with cardiovascular risk factors such as TC,LDL-C,uric acid,CRP,hypertension,diabetes,smoking and drinking.Positive correlation was showed between the stenosis degree of carotid artery and age,smoking and diabetes.Closely correlation was showed between surface fairing of carotid arterial plaque and LDL-C,CRP,smoking and diabetes.The stenosis degree of carotid artery only showed positive correlation with cerebrovascular events,but the morphological features of surface fairing of carotid arterial plaque equally showed positive correlations with cerebrovascular events and cardiovascular events.Conclusions The development of carotid arterial atherosclerosis plaque in the aged is closely related to cardiovascular risk factors.Morphological features of carotid arterial plaque may respond the level of general atherosclerosis better than stenosis degree,and it is also in close correlation with cerebrovascular events and cardiovascular events.

Objective To evaluate the effect of CPB with or without cardioplegia on hemodynamics and myocardial function. Methods Thirty NYHA class III patients undergoing mitral valve replacement were randomly divided into three groups of ten each: group Ⅰ received no blood cardioplegia; group Ⅱ received tepid blood cardioplegia solution and group Ⅲ received cold blood cardioplegia solution. The patients were premedicated with pethidine 50mg and scopolamine 0.3mg. Swan-Ganz catheter was inserted via right interval jugular vein into pulmonary artery and radial artery was cannulated under local anesthesia before anesthesia. Anesthesia was induced with midazolam 0.05-0.01 mg.kg-1, fentanyl 15-20?g. kg-1 and pancuronium 0.12mg.kg-1 and maintained with fentanyl, midazolam and pancuromium. Patients in group Ⅰ received no cardioplegic solution. Ascending aorta was not cross-clamped. Body temperature was reduced to 32℃-33 ℃ (naso-pharyngeal T) . The empty heart was beating at 40-60 bmp. In group D and Ⅲ cardioplegic solution (modified St. Thomas solution) was added to blood from oxygenator (in the proportion of 1:4).In group Ⅱ body temperature was reduced to 32℃-33℃.Tepid (32℃ ) hyperkalemia blood cardioplegic solution was infused at 200-250ml.min-1 after ascending aorta was cross-clamped. After cardioplegic arrest hypokalemic blood cardioplegia solution was infused. In group Ⅲ body temperature was reduced to 28℃-29℃. Cold (8℃) hyperkalemic blood cardioplegic solution was infused at 200-250ml. min-1, after ascending aorta was cross-clamped. After cardioplegia arrest cold hypokalemic blood cardioplegia solution was infused every 20 min. Hemodynamic parameters (MAP, MPAP, CO, CI, SVRI, PVRI, LVSWI and RVSWI) were recorded before anesthesia (T0), before CPB (T1), 10, 30, 60 min after termination of CPB (T2-T4 ) and at the end of surgery (T5 ) . Results Demographic data including age, gender, body weight and body surface area were comparable between the three groups. CI in group 1 and Ⅱ was significantly higher at T2 -T5 than that in group Ⅲ . SVRI after CPB in group Ⅰ was significantly lower than that in group Ⅲ . Conclusion The myocardial function after CPB without cardioplegia is better than that after CPB with cold cardioplegia, but is not significantly different from that after CPB with tepid blood cardioplegia.

Object To study the chemical constituents of the whole plant of Selaginella stauntoniana Spring. Methods Various chromatographic techniques were employed for the isolation and purification of its constituents, and structurally identified by spectral analysis (IR, UV, MS, 1HNMR, 13CNMR) and chemical evidence. Results Four compounds were identified from its extract as: emodin (Ⅰ), ginkgetin (Ⅱ), hinokiflavone (Ⅲ), amentoflavone (Ⅳ). Conclusion All the compounds were isolated in this plant for the first time; compound Ⅰ was found from the plants of Selaginellaceae Beauv. for the first time.

Objective To investigate the effects of Free fatty acids (FFAs) on extracellular matrix(ECM) mRNA expression and secretion.Methods Rat glomerular mesangial cells(HBZY-1 cells) were cultured in vitro and stimulated with OA in different concentration.The expression of collagen Ⅳ(Col Ⅳ) and fibronectin (FN) and transforming growth factor-beta1(TGF-?_1) mRNA were measured by semi-quantitative RT-PCR.The levels of Col Ⅳ and FN in cultured supernatant were determined by ELISA.Results The mRNA expression of Col Ⅳ, FN and TGF-?_1 of 25、100、400 ?mol/L stimulated OA groups were 0.94?0.17、1.16?0.15、1.28?0.19 and 0.82?0.11、0.97?0.07、1.09?0.08 and 1.15? 0.07、1.24?0.06、1.36?0.05 respectively , which increased significantly compared with their control group(0.73?0.16、0.53?0.09、 0.96?0.11 P

OBJECTIVE To review and analyze the distribution and antimicrobial resistance of nosocomial Escherichia coli and Klebsiella pneumoniae.METHODS Disk diffusion test(Kirby-Bauer)was used for anti-microbial susceptibility.Extended spectrum ?-lactamases(ESBLs)were detected by double disk synergy test and disk diffusion confirmatory test.AmpC enzyme was detected by the three dimensional assay.RESULTS The positive rate of the production of ESBLs and AmpC enzyme was 34.6% and 5.0%,respectively.The production rate of both ESBLs and AmpC enzyme positive was 2.4%.Isolated rate of ESBLs-producing strains in sputum and urine of the inpatients in respiratory ward and urinary ward was higher than others.All strains were 100% susceptible to meropenem and imipenem but resistant to 15 other antimicrobials in different degrees.CONCLUSIONS The isolated rate of ?-lactamases-producing strains is increasing year by year.These strains are multi-drug resistant.Attention must be paid to their detection and surveillance.