Objective To improve the clinical diagnosis and prognosis of the treatment of primary liver carcinoma with bone metastasis.Methods A retrospective study on diagnosis and treatment of bone metastasis from 55 cases of primary liver carcinoma was developed.Survival rates were calculated by Kaplan-meier meth-od,univariates analysed by Log-rank and multivariates analysed by Cox regression.Results The 1-,2-and 3-year cumulative survival rates of the cases from primary liver carcinoma were 54.5%,25.5%and 16.4%re-spectively.The mediate survival time was 13 months.And the cases with bone metastasis were 23.6%,10.9%and 1.8%respectively.The mediate survival time was 5.5 months.Factors such as metastasis to other organs,liv-er function,and the combined modality therapy were independent prognostic factors.While number of bone metas-tasis,AFP level and number of liver cancer had no significant relations with the survival rate.Conclusion The prognosis of bone metastasis from primary liver carcinoma is poor.It is important to take emphasis on combined mo-dality therapy,which may be benefit on reducing the symptom,improving the quality and prolong the life span.

Solid tumor growth and metastasis depends on angiogenesis.Anti-angiogenesis cancer therapy research has become one of the hot topics.Studies have shown that many kinds of Chinese medicine have an effectiveness of anti-tumor angiogenesis. It is one of the most important mechanisms to anti-tumor Chinese medicine.Chinese medicine on the anti-tumor angiogenesis research in recent years will be reviewed.

Purpose:To explore the effects and mechanisms o f anti-invasion and anti-metastasis of ginsenoside Rg3 on human hepatocellular carcinoma cells. Methods:To select human hepatocellular carcinoma cell line SMMC -7721 and the human allantoic veins endothelial cell line ECV304 as the study objects. We observed the effet of ginsenoside Rg3’s effect on the growth of SM MC-7721 and ECV304 by MTT methods,the adhesion of SMMC-7721 and Fibronectin by cell adhesion experiment, the expression of gene protein of nm23,CD44 and VEGF by immunohisto-chemical method. Results:Ginsenoside Rg3 could inhibit not only the growth of SM MC-7721 and ECV304 significantly, but also the adhesion of SMMC-7721 and FN. I t might also down-regulate the expression of CD44, VEGF and up-regulate the nm 23 gene expression. Conclusions:Ginsenoside Rg3 can inhibit invasion and metastasis of the hepatic carcinoma cell. The mechanisms are probably that Ginsenoside Rg3 can inhibit the hepatic carcinoma cells’ invasion activity,regulate the expres sion of the gene proteins which are closely related with invasion and metastasis ,inhibit neovasularization.

The occurrence of liver cancer is a multi-phase and multi-factor process, while Chinese medicine used for antitumor is all-round with multiple approaches. This summary is a literature review which elaborates the prevention and treatment of Chinese medicine to the molecular mechanism of liver cancer in eight aspects, such as inhibiting the proliferation of hepatoma cell, inducing their differentiation, affecting the activity of telomerase, resisting the invasion and metastasis of hepatoma cell, inhibiting the formation of tumor vessel, adjusting the immunity function, and reversing the multidrug resistance, etc.
Animals ; Antineoplastic Agents, Phytogenic ; pharmacology ; therapeutic use ; Apoptosis ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Drug Resistance, Multiple ; drug effects ; Drug Resistance, Neoplasm ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Liver Neoplasms ; drug therapy ; Medicine, Chinese Traditional ; Neoplasm Invasiveness ; Telomerase ; drug effects ; metabolism

As the research on the signal transduction pathway of tumor is developing, people has become more aware of the confusion of the signal transduction mechanisms on the tumor cells and their effects on tumor growth, apoptosis, and metastasis. Currently, the research that Chinese medicine and its extract inducing apoptosis and angiogenesis of hepatocellular carcinoma by acting on the signal transduction pathway has made gratifying progress. In this article, we will provide an overview of recent literature about this.
Animals ; Apoptosis ; drug effects ; Carcinoma, Hepatocellular ; pathology ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Liver Neoplasms ; pathology ; Signal Transduction ; drug effects

Adult ; Antineoplastic Combined Chemotherapy Protocols ; adverse effects ; Fluorouracil ; adverse effects ; Hepatitis B virus ; drug effects ; physiology ; Humans ; Leucovorin ; adverse effects ; Liver Neoplasms ; drug therapy ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Organoplatinum Compounds ; adverse effects ; Virus Activation ; drug effects

Objective\ To investigate apoptosis of Hepatoma BEL\|7402 cells induced by allicin. Methods\ The inhibit effect of allicin on BEL\|7402 cells was investigated with MTT assay. Apoptosis was ascertained by cell morphology under light microscope, electron microscope and flow cytometry. Results\ BEL\|7402 cells were suppressed with dose and time dependent relationship after exposed to allicin of 10,20,40 mg/L for vairous lengths of time. Exposed to allicin at 60 mg/L for 3 hours, BEL\|7402 cells showed typical morphology characters of apoptosis: the microvilli of the cell surface disappeared, the cell shrink and decreased in volume and blebs or ball\|like bodies appeared. Typical nuclear condensation, margination and fragmentation were observed. Apoptotic cells were of trypan blue\|rejected staining. Percentage of apoptotic cells of control group and inductive group was 2\^02?0\^37%,78\^48?3\^15% respectively by trypan blue, and 1\^78?0\^48%, 74\^07?3\^94% by flow cytometry. Cells in G\-0/G\-1, S, G\-2/M phases of control group before induction were 47\^66?2\^72%, 22\^06?2\^04%, 30\^25?3\^78% respectively.Each of them is lower than the percentage of apoptotic cells. Conclusion\ Allicin induced apoptosis of hepatoma BEL\|7402 cells.Apoptotic cells were supposed to be initiated in some phases of cell cycle.\;[

Objective:To study the significance of H19 gene in the progress from normal mucosa through oral submucous fibrosis (OSF) to carcinogenesis.Methods:Real-time fluorescent quantitative PCR technique was used to detect LncRNA H19 expression level in 12 cases of normal buccal mucosa tissue,33 cases of OSF buccal mucosa tissue and 31 cases of buccal carcinoma with OSF.Results:The relative expression levels of LncRNA H19 in normal buccal mucosa tissues,OSF buccal mucosa tissue and buccal carcinoma with OSF tissue were 1.17 ±0.37,3.44 ± 1.08 and 8.88 ± 1.78 respectively(between each 2 groups,P ＜ 0.01).Conclusion:H19 may involve the occurrence and canceration of OSF.

The article reviewed the progress in the research of antitumor activities and mechanisms of Marsdenia tenacissima. Its origin producing area and antitumor constituents were analyzed. The herb possessed extensive antitumor effects in experimental and clinical applications. It provides theoretical evidences for study on constituents in this traditional Chinese medicine. Futher studies on the species in M. tenacissima are needed for better medicinal utilization.
Animals ; Antineoplastic Agents ; administration & dosage ; pharmacology ; therapeutic use ; Drugs, Chinese Herbal ; administration & dosage ; pharmacology ; therapeutic use ; Humans ; Marsdenia ; chemistry ; Neoplasms ; drug therapy ; pathology

A comprehensive analytical method based on solid phase extraction-isotope dilution-gas chromatography tandem mass spectrometry has been developed for the determination of musk xylene in cosmetics. Various cosmetic samples, including cream, lotion, powder, shampoo and lipstick, were extracted under ultrasonication. The extract was centrifuged, and the upper solution was concentrated by rotary evaporator. The reconstructed solution was then cleaned up by Sep-Pak Silica solid phase extraction cartridge. Qualitative and quantitative analysis was carried out under the MRM mode after the chromatographic separation on DB-5 MS(30 m×0.25 mm, 0.25 μm) capillary column. The limit of quantitation(LOQ) for musk xylene was 5 μg/kg. The mean recoveries at the three spiked levels of 5-50 μg/kg were 81.1%-86.9%, with the intra-day precision less than 10% and the inter-day precision less than 12%. The method is accurate, rapid, sensitive and adapt to the inspection of musk xylene in cosmetics.