Objective:To analyze routine blood test results of pregnant women at different periods of pregnancy and evaluate the health status of pregnant women.Methods:A total of 240 pregnant women with anemia who underwent antenatal care or delivery in Department of Obstetrics and Gynecology, The People's Hospital of Yuhuan from January 2018 to June 2019 were included in this study. They were divided into three groups according to different periods of pregnancy with 80 women in each group: first trimester, second trimester, and third trimester. Additional 80 non-pregnant women who concurrently received health examination were included as controls. Routine blood test results were compared among groups. The incidence of microcytic anemia and macrocytic anemia at different periods of pregnancy was determined.Results:The incidence of microcytic anemia in the third trimester group [3.75% (3/80)] was significantly lower than that in the first trimester group [26.25% (21/80)] and the second trimester group [25.00% (20/80), χ2 = 15.882, 14.675, both P < 0.05]. The incidence of macrocytic anemia in the third trimester group [22.50% (18/80)] was significantly higher than that in the first trimester group [1.25% (1/80)] and the second trimester group [3.75% (3/80), χ2 = 17.260, 12.333, both P < 0.05]. Red blood cell count and hemoglobin and hematocrit levels in the peripheral blood of pregnant women in the first, second and third trimester groups were significantly lower compared with the control group ( t = 8.579, 16.781, 13.964, 10.154, 15.891 and 15.512, all P < 0.05). Red blood cell count, hemoglobin and hematocrit levels in the second trimester group were significantly lower than those in the other three groups (all P < 0.05). In the first, second and third trimester groups, white blood cell count and percentage of neutrophils were increased, and platelet count was decreased ( t = 23.085, 24.187, 27.941, 15.722, 22.153, 13.277, 31.517, 32.901, 32.227, all P < 0.05) compared with the control group. The increases in white blood cell count and percentage of neutrophils in the second trimester group were significantly greater than those in the first and third trimester groups, and platelet count in the third trimester group was significantly lower than that in the first and second trimester groups (all P < 0.05). Conclusion:Monitoring routine blood indexes can help assess the health status of pregnant women. Adjusting nutrition program in time can decrease the incidence of adverse pregnancy outcomes.

Objective To establish a new cell resource of stem cell transplantation for heart failure therapy,by using cardiomyocytes from rat marrow mesenchymal stem cells(MSCs). Methods MSCs were isolated and cultured from rat bone marrow,then induced by 5aza(5,10,15,20 ?mol/L) for 24 hours.The cultured cells were observed with phase-contrast microscopy.The immunohistochemical technique and laser scanning confocal microscopy(LSCM) were applied for revealing the expressions of desmin,?-sarcomeric actin and C-TnT.The induced cells were evaluated by a transmission electron microscope.GATA4 and(?-MHC) expressions were detected by relative quantitative RT-PCR after 7,21,28 days of inducement respectively. Results MSCs induced in vitro by 5-aza could be identified by the positive staining for desmin,?-sarcomeric actin and C-TnT.The number of cells stained positively in the 5 ?mol/L was greater than that of other groups.Transmission electron microscope showed that paralleled myofilaments were formed.RT-PCR assessment showed that the differentiated cells began to express GATA4 from day 7 to day 28 of differentiation and began to express ?MHC from day 21 to day 28 of differentiation.Conclusion Cardiomyocyte-like cells can be generated from MSCs in vitro and the marrow mesenchymal stem cells are one of the best resources of donor cardiomyocytes.

BACKGROUND: Being a kind of anti-oxidizing agent, vitamin E has the function of eliminating free radical and reducing the free radical injury of antioxidant enzyme in sports, which can alleviate appearance of fatigue and improve sports ability. OBJECTIVE: Though the intervention of vitamin E upon exercise-induced muscle damage (EIMD), the study was designed to investigate the effect of vitamin E on the morphology of EIMD and to provide the experimental basis of research on morphology and mechanism of EIMD. DESIGN, TIME AND SETTING: A randomized block animal experiment was performed in the Key Laboratory of General Administration of Sport of China in Shenyang Sport University and the Microelectronic Laboratory of Chinese Medical University from May to October 2007. MATERIALS: A total of sixteen male Sprague Dawley rats were randomly divided into four groups: control, exercise, normal saline, and vitamin E groups. METHODS: The rats in the vitamin E group was administered (i.p.) 1-1.2 mg/kg vitamin E capsules once every eight hours four times one day before experiment, and the amount of injecting volume was 4 mL/kg. The rats in the normal saline group were administered (i.p.) normal saline with same volume and time. The rats in the exercise group only took some exercises without administering vitamin E or normal saline. The rats in the control group received not any intervention and treatment. The injury model was established by taking a bout of exhaustive downhill running. The triceps brachii muscles extracted from the right side of rats after exercise were prepared for electron microscope specimens. MAIN OUTCOME MEASURES: The arrangement of myofibrils and sarcomeres, the changes of Z-line, and the morphology changes of cell membranes, nucleus, mitochondria, sarcoplasmic reticulum, transverse tubule, and satellite cells were observed. RESULTS: In the vitamin E group, the muscular ultrastructures of EIMD were improved obviously. Though the sarcoplasmic reticulum showed edema, the regular arrangement of myofibrils was observed. The light and dark zone of Z-line and nucleus appeared clearly. CONCLUSION: Supplement of vitamin E could decrease the muscular morphological changes of EIMD after eccentric exercise. The arrangement of myofibrils was more regular and sarcomeres appeared more clearly. The function might relate to the antioxidation and promoting synthesis of proteins of vitamin E.

BACKGROUND:It has been shown to exhibit the increase of free radical has relationship with exercise-induced skeletal muscle injury.As a kind of antioxidant,vitamin E has the ability of removing radicals,which can relieve antioxidase damage,and delay fatigue,eventually,improve the motor ability.OBJECTIVE:From the angle of free radical metabolism,to explore the effect of vitamin E on malondialdehyde(MDA)and superoxide dismutase(SOD)of mitochondria in skeletal muscular cell after eccentric exercise,additionally,to clarify the mechanism of vitamin E on reducing the skeletal muscle damage.DESIGN,TIME AND SETTING:The randomized grouping experiment of animal was carried out in the Key Laboratory of General Administration of Sport of China in Shenyang Sport University and the Key Laboratory of China Medical University from May to October in 2007.MATERIALS:A total of 48 male,Sprague Dawley rats,weighing(304?12)g were randomly divided into the of control,sport,physiological saline,and vitamin E groups,with 12 animals in each group.METHODS:Rats in the vitamin E group was administered(i.p.)1-1.2 mg/kg vitamin E capsules once per eight hours,four times one day before experiment,and the amount of injecting volume was 4 mL/kg.The rats in physiological saline were injected by physiological saline,with the same injection methods of the vitamin E group.The models of injury were established by a bout of exhaustive eccentric exercise on a treadmill.After exercise,triceps brachiies were moved from the right side of rats.MAIN OUTCOME MEASURES:The content of MDA and activity of SOD were measured with the reagent-boxes and 6010 UV-seen spectrophotometer.RESULTS:The values of MDA,SOD of mitochondria in triceps brachiies cells in each group were greatly increased after 24 hour eccentric exercise(P 0.05),the value of MDA in the vitamin E group was greatly decreased(P

It expounds the knowledge of causa morbi and mechanism of insomnia, viewing that it is the result of complex reasons functioning to the body, its attack is to some extent related with individual constitution and characteristics, its mechanism is multiple too, the pathology shows deficiency, heat, sputum and stasis.

Objective:To observe the dynamic changes in serum gastroanteric hormones in patients with stroke complicating stress upper gastrointestinal hemorrhage in acute stage,the changes in acidity,and the changes in gastric empty and to investigate the pathogenetic link of stress pathological change of gastric mucous membrane in patients with stroke(Zhongzangfu,ZZF,中脏腑).Methods:In 20 cases with acute ZZF (Glasgow coma score 6-12 scores) who were all complicating upper gastroenteric bleeding within 24 hours of attack,the levels of motilin(MTL),somatostation(SS),and gastruins(GAS) were measured by radioimmunoassay at the first day (within 24 hours) and the fourth day (within 72-96 hours) respectively meanwhile the gastratube was plased and kept within 24 hours of attack to dynamically observe the pH of gastric juice and the condition of gastric emptying.Results:In comparison with control group the MTL level was significantly increased in patients at the first day of attack (P<0.05),the SS level significantly decreased (P<0.01),and the GSA level had not significant difference (P>0.05).Otherwise the pH (2.87±0.90） of gastric juice in comparison with control group has not significant difference (P>0.05).Among patients in 17 cases the gastric emptying time was prolonged.Even at the fourth day in patients the MTL level was still significantly increased (P<0.01),SS level decreased (P<0.05),and GAS level increased (P<0.01).Conclusions:The upper gastrointestinal hemorrhage in patients with acute ZZF is tightly associated with the changes in MTL and SS but the changes in GAS and gastric pH are not main factor.

Objective: To investigate alveolar bone remodeling on both labial and lingual aspects of lower anterior teeth during treatment and retention stage. Methods: Forty-three cases with lower first premolars extracted (34 girls and 9 boys) and full records at three time points (pretreatment, posttreatment, follow-up) were collected as study sample. Displacement of lower incisor and bone thickness around its labial and lingual aspect were measured. Results:For the level of center of resistance(LC), there was statistically significant decrease of alveolar bone on lingual aspect (0.78?0.77) mm and no change on labial aspect, as the center of resistance(CR) was retracted lingually by (-3.02?1.13) mm during treatment. Analogous changes were found at the level of 3 mm apical to the center of resistance (L3C). In retention stage, with stable positioned lower incisor, no statistically significant change of alveolar bone was found on labial aspect at LC while a little amount of decrease of labial bone (0.20?0.58) mm was found at L3C. On the contrary, subsequential bone apposition could be detected with increase of lingual bone at both levels by (0.31?0.76) mm and (0.38?0.94)mm respectively. Conclusion:Speed of labial bone resorption is faster than that of lingual bone apposition during orthodontic treatment. In retention stage, sequential bone apposition on lingual aspect is presented with lower incisor in stable position.

Objective: To evaluate the effect of dietary fiber and phytic acid inositol hexaphosphate (IP6 or Insp6) on 1,2-dimethylhydrazine(DMH) induced colorectal carcinogenesis in Wistar rats. Methods: 86 four-week-old male Wistar rats were divided randomly into 6 groups and fed with either basal fiber free diet or the basal diet supplemented with 10% pectin, 10% cellulose, 2% Na-InsP6 supplemented water, 10% pectin in combination with 2% Na-InsP6 supplemented water, 10% cellulose in combination with 2% Na-InsP6 supplemented water. After four weeks, the rats were given a weekly injection of DMH (20mg/kg bw) for 20 weeks. The rats were killed after 26 weeks. The number of total macroscopically visible neoplasms was counted and the volume of individual neoplasmas was calculated. Proliferation cell nuclear antigen (PCNA) was analyzed. Results: There was no significant difference between the treatment group and the control group in the incidence of tumors. InsP6 significantly reduced the number of tumors in rats and tumor volume. Pectin and Pectin+InsP6 significantly increased the number of tumors in rats. The expression of proliferation marker PCNA was significantly down-regulated by InsP6 and significantly increased by pectin. Conclusion: A treatment regimen of 2% Na-InsP6 in drinking water was effective in significantly reducing the risk of colorectal cancer in Wistar rats. Diet supplemented with pectin could increase the risk of colorectal cancer.

Objective To investigate the effects of low tidal volume ventilation on pulmonary and cardiac function before and after mitral valve replacement. Methods Thirty patients with mitral valve diseases were randomly divided into traditional tidal volume ventilation (groupⅠ), low tidal volume ventilation with conventional respiratory rate (group Ⅱ), and low tidal volume ventilation with high respiratory rate (group Ⅲ). Mean arterial pressure (MAP), cardiac output(CO), cardiac index(CI), mean pulmonary arterial pressure (MPAP), pulmonary capillary wedge pressure (PCWP), central venous pressure (CVP), systemic vascular resistance index (SVRI), and pulmonary vascular resistance index (PVRI) were monitored. Arterial oxygen tension (PaO_2), carbon dioxide tension (PaCO_2), oxygen saturation index(PaO_2/FiO_2), alveolar-arterial PO_2 gradient (PA-aO_2), and Q_S/Q_T were measured. Results Before CPB, CO in group Ⅰ was significantly lower than that in group Ⅲ (P

OBJECTIVE:To observe therapeutic efficacy and safety of Zhenqi fuzheng granules in the adjunctive treatment of cervical cancer. METHODS:86 patients with cervical cancer were randomly divided into control group(43 cases)and observation group (43 cases). Control group was given intravenous dripping of Diphenhydramine hydrochloride injection 20 mg,Granisetron hydrochloride and glucose injection 5 mg,Cimetidine injection 20 mg at 0.5 h before chemotherapy. After 0.5 h of intravenous drip-ping,the patients received chemotherapy:Docetaxel injection 40 mg/m2,d1,d8,intravenous dripping;Cisplatin for injection 25 mg/m2,d1-3,intravenous dripping;21 d as a cycle,a total of 4-6 cycles. On the basis of control group,observation group was addi-tionally given Zhenqi fuzheng granules 5 g,twice 2 day. Clinical efficacies of 2 groups were observed as well as T cell subsets in-dexes [CD3+,CD4+,CD8+,natural killer cell(NK)],KPS scores,termination,terminal time,the times of chemotherapy and the oc-currence of toxic reaction before and after treatment. RESULTS:The total remission rate of observation group was significantly higher than that of control group;the proportion of termination of chemotherapy in observation group was significantly lower than control group;terminal time of chemotherapy was significantly shorter than control group;the times of chemotherapy was signifi-cantly more than control group;the incidence of gastrointestinal reaction,anemia,thrombocytopenia and leukopenia were signifi-cantly lower than control group,with statistical significance(P<0.05). There was no statistical significance in liver function dam-age,renal function injury and stomatitis between 2 groups (P>0.05). Before treatment,there was no statistical significance in T cell subsets indexes and KPS scores between 2 groups(P>0.05). After treatment,CD3+,CD4+ and NK cells in observation group were significantly higher than before treatment and control group,while CD8+ was significantly lower than before treatment and control group;KPS scores of 2 groups were significantly higher than before,and the observation group was significantly higher than the control group,with statistical significance(P<0.05). There was no statistical significance in T cell subsets indexes of con-trol group before and after treatment(P>0.05). CONCLUSIONS:On the basis of routine treatment,Zhenqi fuzheng granules can improve therapeutic efficacy,immune function and the quality of life,moreover,reduce toxic reaction in patients with cervical can-cer.