1.Quality control of automated blood grouping analyzer in blood group serological detecting
Haiping PAN ; Yun DAI ; Xiaotong SUN
Chinese Medical Equipment Journal 2017;38(3):101-103
Objective To execute quality control of OLYMPUS-PK7300 automated blood grouping analyzer to enhance the quality and efficiency in blood grouping.Methods Measures were proposed from the aspects of specimen quality,reagent quality,instrument maintenance,test parameters setup,internal quality control and external quality assessment,personnel and etc.Results Quality control was carried out at each link of the test by automated blood grouping analyzer to facilitate blood-related clinical operation.Conclusion Whole-course quality control of the analyzer has to be performed to improve blood grouping.
2.Inhibitory effect of curcumin on the expressions of MAPKs and MMPs in RPMI8226 cells during their pro-liferation
Guohua ZHU ; Qi ZHANG ; Haiping DAI ; Qun SHEN
Journal of Medical Postgraduates 2014;(10):1016-1019
Objective curcumin can suppress the proliferation , induce apoptosis and partial differentiation , and inhibit the migration of many kinds of tumor cells .The aim of this study was to investigate the expressions of mitogen-activated protein kinase (MAPKs) and matrix metalloproteinases (MMPs) when the proliferation of human multiple myeloma RPMI8226 cells was inhibited by curcumin in vitro, and to reveal the antitumor molecular mechanism of curcumin . Methods RPMI8226 cells were treated with various concentrations of curcumin for different periods of times .The inhibitory rate of curcumin on cell proliferation was detected by MTT assay , the cell cycle analyzed by flow cytometry , the protein levels of MAPKs measured by Western blot , and the activity of MMPs analyzed by Gelatin zymography . Results Curcumin inhibited the proliferation of RPMI 8226 cells in a time-and dose-dependent manner , and the cell cycle was arrested in the G 2/M phase ([12.72 ±0.68]%vs [4.79 ±0.15]%).The expressions of JNK and p-JNK showed a con-centration-dependent increase in the RPMI8226 cells treated with curcumin at 6.25, 12.50, and 25.00 μmol/L, respectively (P<0.01) , but the expressions of ERK 1/2 and P38 MAPK did not change significantly compared with the control group (P>0.05).In addition, the activities of MMP-2 and MMP-9 were decreased in a dose-depend-ent manner in the supernatant of RPMI8226 cells ( P <0.01). Conclusion A certain concentration of curcumin could not only acti-vate the JNK signalling pathway of the MAPKs family and induce the apoptosis of RPMI8226 cells, but also inhibit the activity of MMPs and influence the invasion and metastasis of RPMI 8226 cells.
3.Several Problems about Drug Management in Drug Clinical Trial Process
Lan CUI ; Lin LV ; Zhiling DAI ; Haiping ZENG
China Pharmacy 2001;0(09):-
OBJECTIVE:To provide reference for drug management in clinical trial process in hospital. METHODS:To summarize the common problems of current drug control in clinical trial process and analyze their harmfulness. Some countermeasures were put forward to resolve those problems. RESULTS & CONCLUSIONS:At present uniform management mode hasn’t been established for drug control in clinical trial institutions. Realizations on the importance of drug control are different from one another. There are several factors which affect clinical trials even result in undesirable consequence. The institution should formulate scientific,effective and practical management standards and operation process to improve the quality of drug control and ensure safety, scientificity and reliability of clinical trials.
4.Experimental study on the differentiation of SHI-1 cells induced by puerariae radix flavones in vitro
Guohua ZHU ; Qi ZHANG ; Haiping DAI ; Yunliang ZHAI ; Qun SHEN
Journal of Leukemia & Lymphoma 2013;22(10):582-585
Objective To explore the possible effects on differentiation of SHI-1 cells induced by puerariae radix flavones(PRF)in vitro.Methods SHI-1 cells were treated with PRF in various concertration,then the inhibitory effects of cell proliferation were detected by MTT assay,the cell cycles were analyzed by flow cytometry(FCM),the cells reduction rates were detected by NBT reduction test,and the expression of CD11b and CD14 were tested by FCM.Results 10-50 μg/ml PRF could inhibit the proliferation of SHI-1 cells in a time-and dose-dependent manner,and the cell cycles were arrested in S phase.When SHI-1 cells were treated with 10,30 and 50 μg/ml PRF in 48 houres respectively,the NBT reduction rates of cells were increased in a dose-dependent with PRF(P<0.05),and the expression of cells surface differentiation antigen CD14 was also increased along with the concentration of PRF.Conclusion The SHI-1 cells could be induced to differentiation partially after treated with 10,30 and 50 μg/ml PRF in vitro.
5.Study of peripherally inserted central venous catheters for parenteral nutrition
Haiping JIANG ; Yake DAI ; Xuehua YANG ; Yanying ZHU
Chinese Journal of General Surgery 2001;0(08):-
0.05).The complications of PICC included phlebitis,catheter occulusion,infection,malposition of catheter,but no pneumothorax or hemopneumothorax occured .Group PICC had higher phlebitis than that in group CVC(P
6.Therapeutic Effects of Itraconazole and Terbinafine for the Treatment of Onychomycosis Evaluated with Scoring Clinical Index
Yan HU ; Lijia YANG ; Xunyi DAI ; Haiping ZHANG ; Ling WEI ; Haikang HUA ; Jia QI ; Hui SUN ; Yuan ZHENG ; Pingdong JIANG
Chinese Journal of Dermatology 2003;0(08):-
0.05).Conclusions The SCIO system is practical to assess the therapeutic effects of itraconazole and terbinafine for patients with onychomycosis.Treatment of onychomycosis with the two drugs is equally effective and safe.
7.Application of antigen retrieval method in hMAM immunohistochemical staining of old paraffin-embedded specimens
Cuimi DUAN ; Xiqin YANG ; Bingshui XIU ; Zhiqiang LIU ; Xuhui ZHANG ; Zhenhua DAI ; Haiping QUE ; Xiaoyan FENG ; Heqiu ZHANG
Military Medical Sciences 2014;(12):965-967,980
Objective To develop an antigen retrieval method for detection of human mammaglobin ( hMAM) immuno-histochemcal staining in old paraffin-embedded specimens .Methods The tissue sections in test group were put into dis-tilled water after deparaffinization and then moved into citric acid buffer ( pH 3.5) for 10-15 min.The other two meth-ods,microwave method and high pressure cooker method ,were compared as control groups at the same time .Finally, immu-nohistochemistry SP method was used to check the antibody in the sections .Results The color appearance in the test group (pH 3.5 citric solution) was better than that of microwave oven and high pressure cooker groups .In the test group, tissue sections were not easily cast off from the slices .Conclusion In this study,we have established a new and simple antigen retrieval method which will contribute to immunohistochemistry technology .
8.SARS-CoV-2 vaccination in patients with lymphoid hematologic malignancies
Yawei JIAO ; Shuting CHEN ; Haiping DAI
Journal of Leukemia & Lymphoma 2023;32(4):249-252
Patients with lymphoid hematologic malignancies have a poor prognosis after developing SARS-CoV-2 infection, and their seropositivity rate after SARS-CoV-2 vaccination is lower than that of the healthy population. Since most clinical trials of SARS-CoV-2 vaccines do not include immunodeficient populations, the safety and efficacy of various types of SARS-CoV-2 vaccines for patients with lymphoid hematologic malignancies are unclear. Therefore, physicians should decide whether patients with lymphoid hematologic malignancies receive SARS-CoV-2 vaccination and the timing, type and dose of vaccine after taking into full consideration the patient's immune status, type of treatment and the risk of SARS-CoV-2 infection.
9.Infrastructure and sanitation practice of Chinese primary and middle schools canteens
DAI Jie, HU Peijin, WANG Junyi, LI Yanhui, XIE Xiaolian, LI Juan, ZHAO Haiping, MA Jun
Chinese Journal of School Health 2019;40(9):1383-1385
Objective:
To understand the current situation of infrastructure construction and sanitation practice of school canteens in China and to provide a reference for improving food safety in school canteen and sanitation management level.
Methods:
Stratified sampling method was adopted to select the surveyed schools. Information regarding school canteen infrastructure and sanitation practice was collected through questionnaires. Chi-square test was used to compare the difference in passing rate on the survey indices among different school types.
Results:
In environmental sanitation, 91.0% of the school canteen was rated as good and in personal hygiene, 92.8% of the employees were rated as good. The overall health level of urban schools was better than that of township schools, and non-boarding schools was better than that of boarding schools, with statistically significant differences(P<0.01). 67.1% of school canteens provided with independent food outlets while 98.7% of school canteens without independent food stores. The qualified rate of washing facilities in the dining area was 94.7%, and the qualified rate of distance between the canteen and the pollution source was 92.5%. Nearly all the employees held canteen catering service license (99.0%) and the health qualification certificate (99.5%). Most of the school canteens (98.7%) established the safety rules and regulations, including the food retention sample system. Nearly 61.5% schools initiated the principal meal program.
Conclusion
Sanitation management in the canteens of primary and secondary schools in China is generally satisfactory, with some canteens still fail to meet the standard requirements. More supervision from schools and relevant government departments (market supervision, health and education departments) is needed to provide students with a clean and heathy campus canteen.
10.Indoor air quality monitoring and management in primary and secondary schools in China
DAI Jie, HU Peijin, LI Yanhui, TAO Ran, WEN Bo, ZHAO Haiping, MA Jun
Chinese Journal of School Health 2019;40(8):1224-1226
Objective:
To investigate indoor air quality monitoring and management in primary and secondary schools, so as to provide scientific basis for health protection of students and healthy school environment.
Methods:
Stratified sampling method was adopted to select schools for investigation. Data of daily ventilation and ventilation was collected from relevant principals of schools through questionnaire survey. Chi-square test was used to analyze the difference of ventilation and ventilation among different types of schools.
Results:
Daily indoor air quality testing indicators: 317 schools (13.2%) have tested the concentration of CO2 in the air environment. Daily detection of CO2 in urban schools was significantly better than that in township schools, and the difference is statistically significant(χ2=72.06, P<0.01); Non-boarding schools were superior than boarding schools(χ2=21.89, P<0.01). The proportion of schools that routinely tested for carbon monoxide, particulate matter and volatile pollutants was 6.5%, 7.5% and 9.3%, respectively. Of the schools that participated in the survey, 80.8% had a daily ventilation system. Among them, 925 schools (38.5%) had a cumulative daily ventilation time of more than 90 minutes in cold season, and 331 schools (13.8%) had a daily ventilation time of less than 30 minutes.
Conclusion
Regulations and standards for school air quality monitoring needs to be improved. The Center for Disease Control and Prevention or other qualified institutions are suggested to lead air quality monitoring in schools testing, creating a healthy learning and living environment for primary and secondary school students.