Objective To investigate the relationship between aging-expectation and leisure-time physical activity among the old adults.Methods Structural interviews were administered to 550 old adults using the Godin Leisure-Time Exercise Questionnaire, Expectation Regarding Aging-12 (WHO-DAS 2.0), Activity of Daily Life-8, and the Geriatric Depression Scale-5.Results There was only 16％ of the old adults whose leisure-time physical activities met the WHO global recommendations.The level of cognitive function expectation was still significant in the regression models statistically even after adjusting the social characteristics,mental health status, and physical function successively (OR=1.024,95％ CI=1.005-1.043;OR=1.024,95％ CI=1.005-1.043;OR=1.029,95％ CI =1.009-1.050, respectively), and both of physical health expectation and mental health expectation were not significant in the three models.Conclusion The leisure-time physical activity of the majority old adults doesn't achieve the WHO global recommendations.Improving the cognitive function expectation of the old adults would be one of the valuable approaches to promote the leisure-time physical activity engagement.

Objective To investigate the suitable mode of induced termination of pregnancy at second trimester for women with scarred uterus.Methods A retrospective study was performed in 120 cases of second trimester pregnant women with scarred uterus,who requested termination of pregnancy in Department of Obstetrics,Ruijin Hospital of Shanghai Jiaotong University from January 2010 to July 2016.The indications of termination of pregnancy were fetal anomaly,serious pregnant complication,intrauterine fetal deaths and personal factors.The interval time of previous operation to this pregnancy were recorded.It was ＜ 2 years in 35 cases and ≥2 years in 85 cases.The patients with normal hepatic function began to take mifepristone combined with ethacridine lactate (group A,62 cases).The method of mifepristone combined with misoprostol was used in the patients who failed to amniotomy to inject ethacridine lactatebecause of oligohydramnios or small gestational age (group B,58 cases).The detail information of every patient was recorded,including age,gestational weeks,gravidity,parity,the mode of previous operation,previous operation time and indication,the mode of induced labor inthis pregnancy,the interval time from administration to uterine contraction,delivery or not and the interval time from induction to delivery,postpartum hemorrhage,the successful rate of induce labor,and placental retention ratio.Results (1) It had no significant difference between groups A and B in age,gravidity,parity and the interval time of previous operation to this pregnancy (P ＞ 0.05).There was significant difference between two groups in gestational weeks of induction (P ＜0.01).(2) It had no significant difference between two groups in successful rate of induction and postpartum hemorrhage (P ＞ 0.05),but the time from induction to regular uterine contractionand delivery in group B was significant shorter than that of group A (P ＜ 0.01).The rate of delivery with 24 hours in group B was 89.66％.It was significant higher than that of group A (22.58％,P ＜0.01).(3) The rate of retained placenta in group A (19.23％,10/52) was significant lower than group B (4g.7g％,20/41),but the ratio of residual of placenta and membranes in group A (75％,39/52) was significant higher than that of group B (48.78％,20/41).It was no significant difference between the over 2 years group and the less 2 years group in the incidence rate of complications including placental retention,residual of placenta and membranes and postpartum hemorrhage.It had no significant difference between two groups in incidence of severe pain (P ＞ 0.05).Conclusions Both of methods of mifepristone combined with misoprostol and the mifepristone combined with ethacridine lactate are feasible to induce second trimester termination of pregnancy for women with scarred uterus.

BACKGROUND:Because of the special and complicated biological characteristics of oocytes, the suitable cyropreservation technology for oocytes faces more various chalenges. However, the uneven survival rate and fertilization, damages of developmental potential of the thawed oocyte stil exist. OBJECTIVE:To introduce the progress in basic and application researches of oocyte cryopreservation technology, and to iluminate the technical defects and thoughts and possible research points to overcome these problems. METHODS: A computer-based search of CNKI and PubMed was performed for articles concerning oocyte cryopreservation from January 2004 to October 2014. The search terms were oocyte, cryopreservation, vitrification in Chinese and English, respectively. The old and repeated articles were excluded. Finaly, 41 articles were included in result analysis. RESULTS AND CONCLUSION:The cryopreservation of oocytes by slow freezing and vitrification has been used in clinic. At the same time, the uneven survival and fertilization rate, damaged developmental potential of the thawed oocyte stil puzzle the clinicians. The key point to breakthrough or improvement of oocyte cryopreservation technology is the systematization of the protocol for oocylte cryopreservation, for example, the choice of cryoprotective agents, the development of carriers for oocytes, and the determination of oocyte stage. Furthermore, other related technologies should also be considered, including thein vitro mature technology of oocytes, cryopreservation and transplantation of ovary tissues.

ObjectiveTo explore the effects of splenectomy on hepatic fibrosis and on the expression of PDGF-B in the liver and PDGF-BB in the serum of rats with hepatic fibrosis. MethodsBy hypodermic injection CCl4, we established 65 rat models with hepatic fibrosis, splenectomies were performed in the three groups at different phases: before hypodermic injection CCl4 (A group), five weeks after hypodermic injection CCl4 (B group), and ten weeks hypodermic injection CCl4 (C group). The control groups were established at the same time, with samples of the livers and serum of the rats taken in different phases. The expressions of PDGF in the liver were detected by immunohistochemistry technique and the degree of hepatic fibrosis was detected by HE staining. The serum levels of PDGF-BB were analyzed by ELISA technique. ResultsAbsorbance values of PDGF-B in the experimental group were significantly lower than the control groups (P＜0. 05). Serum levels of PDGF-BB of the rats after splenectomy were significantly lower than those in the control groups (P＜0.05). HE and Masson's staining showed that the progression of Hepatic fibrosis was slow in the A group. Hepatic pathologic state was significantly relieved in the B group and the inflammation and fibrosis was relieved in the C group. Conclusion Earlier period splenectomy could delay the proceeding of experimental hepatic fibrosis. After splenectomy the decline in the level of PDGF may be one of the mechanisms causing the delay.

Objective To study the relationship between the serum levels of thyroid hormone and the degree of the congestive heart failure as well as the effects of thyroxine on elderly patients with chronic severe congestive heart failure(CHF). Methods 140 cases were divided into group A (60 cases of CHF classes Ⅰ and Ⅱ) and group B (80 cases of CHF classes Ⅲ and Ⅳ) according to the NYHA classification of severity of heart failure. The group B was divided into the observation group (50 cases) and the control group (30 cases) . All patients were treated with conventional methods and thyroxine 20-40 ?g every morning was added in the observation group for 4 weeks. The serum FT 3 ,FT 4 ,TSH,HR,echocardiography and heart function were examined before and after treatment. Results ⑴ Both group A and B had lower base line serum FT 3 and FT 4 ,which were significantly lower in the group B than those in the group A 〔FT 3 : (4.22?0.61) pmol/L vs(3.07?1.10)pmol/L,P

Objective To translate and evaluate the reliability and validity of Chinese version of Nursing Student Self-Efficacy Scale.Methods A total of 212 nursing students in practice were recruited and completed the questionnaire survey including Chinese version of Nursing Student Self-Efficacy Scale and General Self-Efficacy Scale.Results The fitness of confirmatory factor analysis neither in intersectant model nor in orthogonal model were not good.Chinese revised version of Nursing Student Self-Efficacy Scale was formed based on the exploratory factor analysis.The three factors could explain 67.7％ variance of self-efficacy and the loading of all items was above 0.6.The correlation coefficient between the total score and three factors of revised NSSES and GSES was 0.570,0.427,0.582,and 0.480 respectively.The fitness of revised NSSES confirmatory factor of intersectant model,orthogonal model,and modified intersectant model were also not satisfying.Conclusions Although the reliability and the criterion-related validity of revised Chinese version of Nursing Student Self-Efficacy Scale were satisfying,the construct validity was not good and the further revision concerning on the task description and item response grade was required before applying for the internal measurement.

Objective To observe the therapeutic effect of hyaluronic sodium product (SHP) on osteoarthritis(OA).Methods Three hundreds and forty-eight cases were diagnosed as OA and five hundred-eighty knee joints damage caused by OA were treated by intracavitary injection of SHP once a week for three consecutive weeks.The changes of the number of patients with clinical symptoms (joint pain,swelling of joint and with or without difficulty in up and down stairs) and the laboratory test results were compared before and after the treatment.Results It showed that there was a significant difference of the changes of the number of patients with clinical symptoms and laboratory test results before and after the treatment,(P

Objective To observe the curative effect of Modified Gongwaiyun Ⅱ on extrauterine pregnancy.Methods 69 patients of extranterine pregnancy were recruited into a treatment group and a control group randomly. 34 patients in the treatment group were treated with modified Gongwaiyun Ⅱ and 35 patients in the control group were treated with Gongwaiyun Ⅱ, with therapeutic course being one week. Values of β-HCG of serum at the beginning of the treatment,age, days of menelipsis, effective rate, average value of serum β-HCG decreasing and percentage at every course of the treatment, and the number of therapeutic courses were observed. Results There are significant statistic differences between the treatment group and the control group at the number of therapeutic courses, and average value of serum β-HCG decreasing and percentage at every course of the treatment, with P＜0.05. Conclusion The curative effect of modified Gongwaiyun Ⅱ had superiority to traditional Gongwaiyun Ⅱ.

Objective To develop an HPLC method for determination of paeoniflorin in Fufang Chishao Granula. Methods The separation was performed on a Waters C18 column (250 mm× 4.6 mm, 5μm) with acetonitrile-water (30∶70) as the mobile phase at the flow rate of 1.0 mL/min. The detection wavelength was set at 230 nm. Results Paeoniflorin showed good linearity (r=0.999 9) in the range of 0.266-5.32 μg, and the average recovery was 98.00%, RSD=2.22%. Conclusion The method is convenient, sensitive, accurate and repeatable. It can be applied to determination of paeoniflorin in Fufang Chishao Granula and control the quality of the preparation.

In order to establish a simple and useful way for preimplantation genetic diagnosis (PGD) of chromosomal diseases in general IVF laboratory, the methods that are most commonly used in the embryo biopsy, fixation of blastomere and fluorescence in situ hybridization were compared. The three aspects of PGD were analyzed respectively. There was no significant difference in further development capacity of embryos between mechanical (79.7%) and chemical biopsy group (78.6%) (P>0.05). In this study, more cells were successfully fixed with the Tween/HCL method (93.8%) than with the methanol/acetic acid method (80.5%, P<0.05). There was no significant difference in cytoplasm remains between methanol/acetic acid method and Tween/HCL method (P>0.05). The hybridization efficiency of fluorescence in situ hybridization was 89.5% in successive denaturation method and 90.9% in codenaturation method with the difference being not significant (P>0.05). In conclusion, the mechanical or chemical method, Tween/HCL fixation method and codenaturation fluorescence in situ hybridization method can constitute a simple and useful way for PGD of chromosomal diseases.