On the basis of ARMT core processor, a biological signal acquisition system with USB2.0 interface is presented in this paper. It is self-developed and Lab VIEW7 is taken as a host communicating software platform for the entire data communication system. The study is mainly focused on the firmware programming for USB chip, the development of USB WDM Driver, and the method for the calling of DLL from Lab VIEW7 to communicate with self-made hardware device.

AIM: To observe the efficacy of intravitreal injection of conbercept for diabetic macular edema(DME)with different patterns of optical coherence tomography(OCT).

METHODS: A total of 96 patients(96 eyes)with DME were classified as diffuse retinal thickening(DRT group, 35 eyes), cystoid macular edema(CME group, 33 eyes)and serous retinal detachment(SRD group, 28 eyes)according to the OCT. All patients were treated with intravitreal injection of 0.5mg(0.05mL)conbercept. The changes of best corrected visual acuity(BCVA), central foveal thickness(CFT), the injection times and vision improves eyes were compared between three groups after 1,3,6mo treatment.

RESULTS: After 6mo follow up, the BCVA of the three groups showed a significant downward trend(F_time=205.880, P_time<0.01), and there were significant differences among the three groups(F_group=3.472, P_group=0.042). The DRT group had the best BCVA after treatment. The CFT of the three groups showed a significant downward trend(F_time=392.994, P_time<0.01), and there were significant differences among the three groups(F_group=5.046, P_group=0.012). The reduction of CFT in the DRT group and CME group were better than that in the SRD group. The DRT group had the least injection times, and the highest proportion of vision improves eyes after 6mo follow up.

CONCLUSION: The intravitreal injection of conbercept could significantly improve the visual acuity and reduce the CFT of DME with different OCT patterns. And DRT is the most effective type with the least injection times.

There is a rare case of an elderly diabetic with diabetic foot infection at Hainan General Hospital in September 2021, which was diagnosed as Corynebacterium diphtheriae infection incidentally on routine culture with conventional methods and molecular biological approaches, to aid in diagnosis in clinical practice. Owing to smear staining, Albert staining and VITEK 2 system, automated identification systems viz matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) confirmed combing with 16S ribosomal RNA (16S rRNA) gene has been used for the taxonomic classification of bacteria. Otherwise, toxin gene tox was done for diphtheria toxin synthesis. The isolate was Gram-stain-positive, rod-like arrangement with irregular thickness, with characteristic metachromatic granules, ferment most sugars and homology of 16S rRNA analyses with C. diphtheriae NCTC11397^T (MW682323.1) was greater than a 100% possibility, toxin gene tox was negative. The findings lay the foundation to clinical identify and trace of non-toxigenic C. diphtheriae. Moreover, this work provides insights into the non-toxigenic C.diphtheriae that contribute to recognized risk of non-toxigenic C.diphtheriae infections.
Aged ; Corynebacterium/genetics* ; Corynebacterium diphtheriae/genetics* ; Diabetes Mellitus ; Diabetic Foot ; Diphtheria/microbiology* ; Humans ; RNA, Ribosomal, 16S/genetics*

BACKGROUNDThe one-step method was routine practices in China, scientific evidence to support this intervention is scarce. The purpose of this study was to observe the natural process of head-to-body delivery interval by waiting for at least one contraction (two-step) after head delivered in normal birth.

METHODSFrom March 1 to March 30 in 2015 at Haikou Maternal and Child Hospital in China, normal vaginal birth with normal baby condition were recorded by video. Videotapes were transferred to computer then replayed and observed.

RESULTSNinety-two cases were enrolled in this study. The average head-to-body delivery interval by two-step delivery was 71.04 ± 61.02 s, (mean + 2 standard deviation = 193.07 s, 95% confidence interval [15.65-229.15] s). Fifty-one patients (51/92, 55.43%) were <60 s, 41 patients (41/92, 44.57%) were over 60 s. Shoulders delivered at the first contraction were 96.74% (89/92), 3.26% (3/92) had delivered by the second contraction. Shoulders emerged from perineum were 71.73% (66/92), 15.21% (14/92) transversely, and 13.04% (12/92) emerged from under pubic arch. Babies cried before the shoulder were 31.52% (29/92), cried after birth 52.17% (48/92), and 16.30% (15/92) did not cry after birth. Baby activities included as making faces, sucking, and bubbled from mouth and noses, and the lighter blue color of skin with good perfusion.

CONCLUSIONSThe average time of head-to-body delivery interval was longer than 60 s by two-step delivery. Majority shoulders were delivered at the first contraction. Majority shoulders emerged from perineum rather from under pubic arch. The routine one-step method of shoulder delivery where the downward force applied is not necessary and is not the right direction. Baby's breath, making faces, sucking, bubble from noses and mouth, and the light blue color of the faces, all those signs during shoulder delivery indicated a normal live birth.

Adult ; Delivery, Obstetric ; Female ; Humans ; Infant, Newborn ; Pregnancy ; Reference Values ; Shoulder ; Time Factors ; Uterine Contraction

β-Thalassemia caused by abnormal coding of the β-globin gene is the most common hemoglobinopathy in many Asian countries. The in-depth study of the molecular basis and epigenetic mechanism of globin gene expression is the key to explore a new treatment for thalassemia. In this study, FAIRE (formaldehyde-assisted isolation of regulatory elements), 3C (chromosome conformation capture) and ChIP (Chromatin Immunoprecipitation) were used to investigate the three-dimensional interaction network of β-globin family gene loci and the molecular mechanism of functional regulation of gene expression during rapamycin-induced chromatin remodeling in CD4+ T cells. The results showed that the opening degree of globin gene chromatin, the interaction frequency between the gene promoter region and the regulatory element LCR (Locus control regions), and the enrichment efficiency of CTCF (CCCTC-binding factor) in the gene promoter region changed differently during the change of rapamycin treatment concentration from low to high, which led to the same change trend of the gene expression pattern. At the 10 nmol/ L concentration, chromatin accessibility and gene expression decreased (P < 0. 05). At 20 nmol/ L and 50 nmol/ L concentrations, chromatin accessibility increased and gene expression was up-regulated (P < 0. 05). In this study, the molecular mechanism of gene expression regulation of the β-globin family was expounded through this dynamic change process. Our work provides a theoretical and clinical practice basis for clinical precision treatment.

Objective:To observe the effect of Jiajian Fuyuan Huoxuetang combined with electroacupuncture on neurological rehabilitation of patients with spinal cord injury, and to investigate its effect on neurotrophic and inflammatory factors. Method:One hundred and twenty patients were randomly divided into control group (59 cases) and observation group (61 cases) by random number table. Both groups’ patients got monosialotetrahexosylganglioside by intravenous drip for 6 weeks, 200-400 mg/time, 1 time/day.Rat nerve growth factor for injection by intramuscular injection for 4 weeks, 20 μg/time, 1 time/day. Patients in control group additionally got electroacupuncture treatment,and tongluo huatan capsule,3 caps/time,3 time/days. The patients in observation group additionally got Jiajian Fuyuan Huoxuetang combined with electroacupuncture, 1 dose/day. The courses of treatment were 12 weeks in two groups. Scores of degree of spinal cord neurological impairment were graded by using impairment score of American Spinal Injury Association (ASIA). Lower extremity motor ability was discussed by using the walking index of spinal cord injuryⅡ (WISCⅠ Ⅱ) and 10 minutes’ walking time (10 MWT). Before and after treatment, functions of daily life were evaluated by using Barthel index (MBI). Bladder function was also discussed, and levels of brain-derived nerve growth factor in peripheral blood (BDNF), nerve growth factor (NGF), astrogenic calcium binding protein S-100 β (S-100 β), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were also detected. Result:After the treatment, scores of sensory and motor in ASIA scale in observation group were all higher than those in control group (P<0.01). Score of WISCⅠ Ⅱ in observation group was higher than that in control group, and 10 MWT was shorter than that in control group (P<0.01). Times of leakage of urine, urinary incontinence volume and residual urine volume were all less than those in control group, and bladder volume was more than that in control group (P<0.01). Functions of daily life in observation group were better than those in control group (Z=1.967, P<0.05), and the levels of BDNF and NGF were higher than those in control group, while levels of S-100 β, TNF-α and IL-1β were lower than those in control group (P<0.01). Conclusion:On the basis of routine comprehensive rehabilitation therapy of western medicine, Jiajian Fuyuan Huoxuetang combined with electroacupuncture can promote the recovery of nervous function, improve sensory and motor function, improve bladder function and daily living ability, and promote the expression of neurotrophic factors and inhibit inflammatory reaction in neurological rehabilitation for the patients with spinal cord injury.