Objective: TO establish methods for sensitive, rapid, economical detection of Th1/Th2 Cytokine gene expression. Method: sixpairs of Primers specific for the IL-2, IL-4, IL-10, IL-13, IFNr and B-actin were designed. A Sensitive and rapid method for detection of Th1/Th2cytokine genes by RT-RCR was established. Meanwhile another method: the RNA hybridization with sir cDNA probes were established. Results:The RT-PCR reaction and RNA hybridization was used to test five kinds of Cytokine mRNAs expressed 20 tumor cell lines on mRNAs were expressed in eleven Cell lines;The mRNAs in eight cell lines. Conclusion:The preliminary results suggested that RT-PCR and RNA hybridizationnight be used to test Th1/Th2 cytokine gene expression.

The paper makes a brief introduction to the application of natural language processing in Traditional Chinese Medicine (TCM).Through research and analysis of relevant literatures , it describes the features and application directions of such methods as the association rule mining , clustering analysis , information extraction , machine learning , etc.It also summarizes methods related to the es-tablishment of knowledge networks on TCM and proposes new ideas for future researches of natural language processing in TCM based on the establishment of knowledge networks .

Objective To evaluate the clinical value of using three-dimensional reconstruction and incising software in facial fracture. Methods Twenty-nine patients of facial fracture were examined with spiral CT first and then the images were taken with three-dimensional reconstruction and incising software on work-station. 2D images, 3D images and incising images were evaluated. Results BZ 3D images were superior to 2D images in demonstrating the space relationship of facial fractures, such as the fracture location, range, fragments displacement. However 2D images were better in illustrating deep structure; The 3D images could illustrate deep structure as well as 2D images when combined with incising software. Conclusion 3D images can provide information of space relationship in assessing facial fracture. The range can be extended combining with the incising software, and do better for illustrating deep struction.

Eight-year program education is the heated topic in the field of medical education across the country.The system of eight-year program education can be discussed as a system,from the view of system philosophy.We analyzed beth internal relationships and external relationships connected to the system,so as to look upon the eight-year program education in its entirety.And then we discussed about the problems of epistemology and methodology on that basis.

Clinical tutor system is a unique exploration and practice with characteristics of our school,which has been carried out in the process of clinical course learning and clinical practice among eight-year-program clinical majors.Through analyzing and summarizing the implementation among 2005 and 2006 grade students,we found that the clinical tutor played an active role in cultivating students' clinical thoughts and personal ethics and in improving clinical ability and scientific ability.However,some problem were existed in tutors themselves-specialized major,busy,unclear cultivation objective,etc.Therefore,further improvement of the system was needed in order to better meet the needs of the eight-year-program medical talent training and to provide related references.

Objective To study the effects of cultivation methods on growth dynamics in the aerial part and root of Angelica sinensis and Ditylenchus destructor, rate of early bolting, and yield of A. sinensis as well. Methods Compared with conventional planting, ridge planting, furrow planting, white plastic mulch coverage, and black plastic mulch coverage were used to evaluate the effects of cultivation methods on dry matter accumulating and growth dynamics of A. sinensis. Results The influence of black plastic mulch coverage to growth dynamics of A. sinensis is the biggest, next for white plastic mulch coverage. There is the best inhibition to D. destructor under ridge planting. Black plastic mulch coverage can enhance the rate of early bolting. The treatment of black plastic mulch coverage has the highest yield, which can increase the yield by 33.4% (4 650 kg/hm2) compared to conventional planting; next for the treatment of white plastic mulch coverage, which increased the yield by 24.3% (3 372.2 kg/hm2) compared to conventional planting. But there were no significant effects of ridge planting and furrow planting on increasing yield. Conclusion The cultivation methods of black plastic mulch coverage should be brought into wide use in A. sinensis cultivation.

Objective To construct the RNAi eukaryotic vector of inhibitory member of the ASPP family(iASPP) gene and observe the interfering effect in Jurkat cell line after the vector transfection.Methods The specific siRNA sequence was designed according to the iASPP sequence in GenBank.The sequence was cloned into PGCsilencer~(TM) H1/Neo/GFP and then sequence analysis was performed.The recombinant plasmid was transfected into Jurkat cell by liposome.The iASPP expression was analyzed by RT-PCR and cell apoptosis was detected by FCM.Results The sequence of templet and specific siRNA was correct by sequence analysis.The iASPP expression in Jurkat cells decreased and the apoptosis rate increased from 11.81% to 33.15% after RNAi transfection.Conclusion The RNAi eukaryotic vector PGCsilencer~(TM)H1/Neo/GFP/RNAi was constructed successfully.The RNAi inhibitory effect on the Jurkat cells is confirmed.The successful construction of iASPP RNAi makes it possible to further study the interaction between iASPP and p53.

Objective To investigate the role of interferon regulatory factor-1 (IRF-1) in liver ischemia/reperfusion (IR) injury and its underlying mechanism,and identify effective managements in alleviating liver IR injury.Methods Three groups of mice models with liver IR injury were well established,including control group (S),warm liver IR injury group (IR) and recombinant IRF-1 group (IRF-1).The levels of mRNA and protein,liver function and pathological changes of liver tissue were detected in group S and group IR.Additionally,the marker of IRF-1,p-Stat1,p-P38,PARP1 and Caspase-3 were measured and PCNA expression was determined in group IR and group IRF-1 mice with 6-hour liver IR injury.Results IRF-1 mRNA and protein and the levels expression of proteins were significantly elevated with peak occurred after 6-hour IR injury,which was statistic difference compare to the group S (t2h =-3.512,t6h =-4.247,t12h =-4.088,t24h =-3.851;P ＜ 0.05).Serum ALT and AST of mice detected in group IR were higher than group S at all endpoints (tALT =4.931,4.592,4.277,4.809;tAST =4.980,4.617,4.336,4.915;P ＜ 0.05).Furthermore,pathological damage change was more distinct compared with group S.The elevated levels of IRF-1,p-Statl,p-P38,PARP1 and Caspase-3 and decreased PCNA expression were determined in mice models with recombinant IRF-1 intervention.Conclusion IRF-1 expression could be closely correlated with liver IR injury,and its underlying mechanism may be attributed to activation of JNK MAPK protein and inhibition of PCNA expression.

Objective To observe the clinical efficacy of moxibustion plus anus-lifting exercise in preventing hemorrhoids relapse.Method Seventy eligible patients with mixed hemorrhoids whose symptoms had been released by conservative treatment were randomized into a treatment group and a control group, 35 cases in each group. The treatment group was intervened by moxibustion plus anus-lifting exercise, while the control group only practiced anus-lifting exercise. The intervention was conducted successively for 4 weeks. Symptoms including hematochezia, bearing-down pain, prolapse, and size of hemorrhoid were scored in the 1st, 2nd, 3rd, and 4th treatment weeks as well as the 4th week after the intervention, meanwhile, the relapse rates of the above symptoms were observed.Result In the 3rd treatment week, there were significant differences in comparing the scores of prolapse, bearing-down pain, and hemorrhoid size between the two groups (allP<0.05); in the 4th treatment week and the 4th week after the intervention, there were significant differences in comparing the scores of hematochezia, prolapse, bearing-down pain and hemorrhoid size between the two groups (allP<0.05); in the 4th week after the intervention, there were significant differences in comparing the relapse rates of hematochezia, bearing-down pain, prolapse and hemorrhoid size between the two groups (P<0.05).Conclusion Moxibustion plus anus-lifting exercise can produce a significant efficacy in preventing the hemorrhoids relapse.

Objective To evaluate the accuracy of MRI diagnosis and the clinical value of multi-MR imaging techniques.Methods Clinical,pathologic and MRI findings were retrospectively reviewed in 25 cases with chronic pancreatitis.The MRI manifestation included pancreatic morphology and volume change,signal feature,dynamic contrast-enhanced MRI and MRCP.The MRI techniques included: both before and after Gd-DTPA enhancement FS FLASH T1WI,FLASH TIWI,HASTE T2WI and MRCP.Results The characteristic signs in diagnosis of chronic pancreatitis were as follows: diffuse enlargement of the body;hypointense on T1WI FS;dynamic contrast-enhanced MRI,lesion and normal area always enhanced at the same time;pancreatic duct dilated like a string of beads which penetrated into the lesion shown by MRCP.Conclusion MRI can detect chronic pancreatitis earlier than CT,and it can display pancreatic duct and pseudo-cysts so is of great value in the diagnoses of chronic pancreatitis.