It makes review on the application of CD-DST on clinical treatment and sci-research of the carcinoma of large intestine from its technological principle and characteristics.It views that as an advanced test technology of medical allergy in vitro, there’s successful report in clinical treatment and concerned scientific study of tumor, however, it’s mainly used in lung cancer and gynecological tumor, the application in the carcinoma of large intestine waits for promotion.

Objective To discuss the causes,agents and treatment of pancreatic encephalopathy in acute pancreatitis.Method We reviewed 26 cases of acute pancreatitis combined with pancreatic encephalopathy within recent 10 years.Results Pancreatic encephalopathy occurred always accompanied with such agents as hyperpyrexia, waterelectrolyte disturbance,hypoxemia, azotemia and bloodsugar disturbance etc.Conclusions The occurrence of the pancreatic encephalopathy is based on the harm that pancreatin does to the brain.The causes of pancreatic encephalopathy vary.To inhibit the releasing of pancreatin is the principle to prevent pancreatic encephalopathy, and to maintain normal physiological function,to control infection and nutritional support are the important links to prevent the pancreatic encephalopathy from happening.

ObjectiveTo evaluate result of palliative operation for hilar cholangiocarcinoma. Methods The clinical data of 179 cases of cholangiocarcinoma during the last 20 years were analysed retrospectively. Results The operative mortality rate was 10 1%, there was no significant difference between the groups. The rate of cholangitis after operation in the Roux en Y choledochjejunostomy group (15 1%) and bridge internal drainage group (10 0%) was significantly lower than that of PTCD (or ERBD) internal drainage group (35 7%, P

Objective In order to discuss the surgical treatment of iatrogenic bile duct injury.Methods 156 cases of iatrogenic bile duct injury during recent 20 years were reviewed and analyzed in this paper.Results The total re-operation rate was 85.71% (126/147), the re-operation rate of LC, OC, OC plus exploration of common bile duct were 95.24%, 87.27%, 62.50% respectively (P

Objective To investigate the effect of minimally invasive surgery in patients with midge-upper advanced gastric cancer (AGC). Methods 167 patients with middle-upper AGC underwent total gastrectomy+D2 lymph node dissection from January 2009 to December 2013 were enrolled in the study and divided into laparoscopic assisted total gastrectomy (LATG) group (n = 57) and open total gastrectomy (OTG) group (n = 110). Baseline data, operative, postoperative data and follow-up result were compared between the two groups. Results There were no significant differences in baseline data between the two groups (P > 0.05); The intraoperative blood loss and incision length in LATG group were significantly lower than that in OTG group (P < 0.05). There were no significant differences in operation time, ratio of intraoperative blood transfusion, distance between the upper margin and the tumor, the number of lymph node dissection between two groups (P > 0.05); Postoperative first aerofluxus time, ecovery liquid diet time, postoperative hospitalization days and VAS score of pain in 3 d after operation in LATG group were significantly lower than that in OTG group (P < 0.05). There was no significant differences in incidences of postoperative complication between two groups (P > 0.05); There was no patient dying within 30 d after surgery.The median follow-up time was 38.2 months (1~58 months). Postoperative 1, 3 year overall survival rates in LATG group and OTG group were 87.7% and 83.6%, 71.9% and 64.5%, and there were no significant differences between the two groups (P > 0.05). Conclusion For patients with middle-upper AGC, LATG+D2 lymph node dissection has lots of advantages including less intraoperative blood loss, shorter incision, faster postoperative recovery, shorter hospital stay and so on, which is safe and effective, and their short-and long-term results are satisfactory.

AIM:To establish a HPLC method for simultaneous determination of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby.METHODS:Using Diamonsil C_ 18(4.6 mm ?250 mm,5?m)as analytical column.The mobile phase consisted of acetonitrile(A)and 0.1% phosphoric acid(B)with gradient elution:0~18 min,83%~80%B;18~30 min,80%~86%B.The detection wavelength of rutin and naringenin-7-o-glucoside was at 254 nm and 281 nm,respectively.The flow rate was 1.0 mL/min;Column temperature was at 35 ℃.RESULTS :A better separating effect was obtained with the HPLC gradient elution method.The linear calibration curve of rutin and naringenin-7-o-glucoside were obtained in the concentration range of 1.00~48.00 ?g/mL(r=0.999 3)and 0.64~40.72 ?g/mL(r=0.999 8),respectively.CONCLUSION:The HPLC method is accurate,simple and can be used to determine the contents of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby simultaneously.

Pancreatic neuroendocrine neoplasms (pNENs) are clinically rare,however,it shows an in creasing trend recent years.Surgical management,such as radical resection or debulking,plays an important role in this field.Different procedures can be applied to tumors with different types,locations,and developing stages.The optimal clinical management of pNENs involves a multidisciplinary approach in order to improve prognosis.

Objective To explore the selection of palliate operation styles for unresectable malignant obstructive jaundice diseases. Methods The clinical data of 112 cases of unresectable malignant obstructive jaundice diseases in the last 5 years were analyzed retrospectively. Results The rate of operation mortality was 7.1％ and the incidence rate of post-operative cholangitis was 17.3 ％.The survival time in the Roux-en-Y choledochojejunostomy group was (9.4±1.6) months, and there were no significant differences among laparotomy stent internal drainage group[(9.8±12.5)months]and the PTCD stent internal drainage group [(9.0± 3.1)months]. But survival time in the laparotomy bridge internal drainage group [(6.8±1.7)months]was significantly lower (P＜0.05). The survival times in the ERCP stent drainage group [(3.5±2.2)months]and exploratory laparotomy group [(2.8±2.7)months]were even more significantly lower (P＜0.01).Conclusion As a palliative operation for unresectable malignant obstructive jaundice diseases, Roux-en-Y choledochojejunostomy applies to the middle and distal obstruction,laparotomy stent internal drainage applies to hilar obstruction,ERCP stent drainage only applies to distal obstruction,and PTCD stent internal drainage applies to any part obstruction of the bile duct. The Roux-en-Y choledochojejunostomy, laparotomy stent internal drainage, and PTCD stent internal drainage would improve the life time and life quality of these patients.

AIM : To establish a HPLC method for simultaneous determination of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby.METHODS: Using Diamonsil C_(18) (4.6 mm×250 mm,5 μm) as analytical column.The mobile phase consisted of acetonitrile (A) and 0.1% phosphoric acid ( B ) with gradient elution : 0～18rain,83%～80% B; 18～30 min,80%～86% B.The detection wavelength of rutin and naringenin-7-o-glucoside was at 254 nm and 281 nm,respectively.The flow rate was 1.0 mL/min; Column temperature was at 35 ℃.RESULTS: A better separating effect was obtained with the HPLC gradient elution method.The linear calibration curve of rutin and naringenin-7-o-glucoside were obtained in the concentration range of 1.00～48.00 μg/mL( r =0.999 3 ) and 0.64 ～ 40.72 μg/mL( r = 0.999 8 ),respectively.CONCLUSION : The HPLC method is accurate,simple and can be used to determine the contents of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby simultaneously.

Objective Since the discovery of multiple bioactivities of agarobiose oligomers, the establishment of quantitative analysis of agarobiose oligomers obtained under different hydrolysis modes would be of much importance. Methods Agarobiose oligomers ranged from biose, tetraose, hexaose, octaose, to decaose were isolated and purified by gel chromatography on Sephadex column. The agarobiose oligomers were separated in RP-HPLC after introducing ?-naphthylamine into compounds. Results Each oligomer could be quantified with good linearity and the lowest detection limit reached 0.1～2 ?g?mL -1. The chromatograms profiles under different hydrolysis modes(HCl, citric acid, solid acid, and hydroxyl radical degradation)showed that the content of agarobiose could reach more than 57.8 % using solid acid hydrolysis with best yield of 33.2 % . While HCl could degrade agar into a series of agaro-oligosaccharides from biose to decaose. The yield of oligosaccharides was low if hydrated by citric acid. The method of Fenton degradation could increase the speed of hydrolysis, but the products were more complex. Conclusion The method established here could be useful for analysis of hydrolysates of oligomers under different hydrolysis modes.