1.Effect of Fengbei Huayu Capsule on FasL Gene Expression and T Cell Apoptosis of Type 2 Diabetic Rats
Haimin GONG ; Wenzhuo DUAN ; Guangxiang JIN
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(07):-
Objective To study the effect of Fengbei Huayu capsule on FasL gene expression and T cell apoptosis of type 2 diabetic rats, and to compare the pathological lesions of kidney and retina after treatment of traditional medicine. Methods To make the model of type 2 diabetic rats and determine the indexes of FasL gene expression and T cell apoptosis rate by RT-PCR, DNA gel electrophoresis methods. Results Compared the FasL gene expression and T cell apoptosis rate, TCM treatment group was significantly lower (P 0.05). Comparison of FasL gene express, TCM treatment group was significantly lower (P
2.Fingerprint of Rhizoma Polygoni Cuspidati
Haimin LEI ; Wei BI ; Qiang LI ; Weihong GONG ; Xian HUANG ; Yuxin ZHOU
Chinese Traditional and Herbal Drugs 1994;0(07):-
Objective To study a fingerprint of raw material of Rhizoma Polygoni Cuspidati from(various) habitats by RP-HPLC. Methods Kromasil C_(18) column(150 mm?4.6 mm,5 ?m) was used,with mixtures of acetonitrile and water as mobile phase in a gradient mode.The flow rate was 1 mL/min and wavelength was 303 nm.Results According to the optimum chromatographic conditions,a good fingerprint of Rhizoma Polygoni Cuspidati has been described.Conclusion The method is simple and accurate with good reproducibility.It may be practical value for the quality control of sample for Rhizoma Polygoni Cuspidati from various habitats.
3.Effect of hyperglycemia on the expressive type transformation of vascular smooth muscle cell in rats
Wenzhuo DUAN ; Jiafu WANG ; Yi DING ; Tongmei LIU ; Xiuyuan SONG ; Jianying WANG ; Haimin GONG ; Chaoshu TANG ; Xia LI
Chinese Journal of Pathophysiology 1986;0(01):-
AIM: To make hyperglycemia models and observe the effects of hyperglycemia on expressive type transformation of vascular smooth muscle cell (VSMC) in rats METHODS: Using tissue culture and radioactivity analysis methods RESULTS:①Hyperglycemia group was lower than control group in NO - 2 content, nitric oxide synthase activity and cGMP content;②Hyperglycemia group was higher than control group in endothelin, insulin, total chdesterol and [ 3H]-TdR penetrated rate of VSMC CONCLUSION: It was possible that hyperglycemia reduces the expressive type of VSMC to change and promotes VSMC proliferation