Objective: To investigate the effects of processing on contens of saponin of Leonurus heterophyllus sweet. Methods: The determinations of saponin were carried out by TLC. Results: There were obvious differences between the crude and the different processed products of Leonurus heterophyllus sweet in contents of saponin, and there were no obvious changes in constituents of saponin.Conclusion: The different processing methods and temperature had great effects on the contents of saponin of Leonurus heterophyllus sweet.