Bone marrow mesenchymal stem cells (BMSCs) is a good seed cell,and it is widely used in intervertebral disc tissue engineering research.The improvement of cellular scaffolds,cell factors and further understanding of intervertebral disc (IVD) cell pathophysiology and BMSCs biology have laid the foundation for cell-based therapies for IVD degeneration.However,BMSCs repairing IVD is still in the early stage of research,at present there are still many problems,one of the most prominent is the survival of transplanted cells.This review mainly introduces the current research status of BMSCs in IVD tissue engineering.

Adjuvant therapy of breast cancer in the child-bearing period tends to affect ovarian function of patients.Adjuvant chemotherapy has a great damage for ovarian function,and tamoxifen's extent of the damage is not yet clear,but gonadotropin-releasing hormone (GnRH) has a protective effect on ovarian function.In the adjuvant therapy,synthetic weigh the pros and cons,make the best individualized programs,is the future direction of the treatment of breast cancer.

Objective To compare the hemodynamic response to orotracheal intubation by Macintosh laryngoscopy (MAC) with Shikani optical stylet (SOS). Methods Forty neurosurgical patients, ASA physical status Ⅰ~Ⅲ, were prospectively randomized to MAC group (n = 20) and SOS group (n = 20) according to the method of orotracheal intubation. Heart rate (HR) and invasive systolic blood pressure (SBP) were recorded at pre- and post-intubation. Results The intubation time was significantly longer in SOS group than in MAC group [(22 ± 8) s vs. (17 ± 8) s, P < 0.05]. SBP in both groups decreased significantly after induction (P < 0.05) and increased greatly at 1 and 2 min post-intubation (P < 0.05), but did not differ at 3, 4 and 5 min. HR in both groups did not differ significantly between the SBP after induction. HR in both groups increased greatly at 1 and 2 min post-intubation (P < 0.05), but did not differ markedly at 3, 4 and 5 min after induction. There were no significant differences in the peak SBP and HR between the two groups. Conclusion MAC and SOS cause similar hemodynamic responses to orotracheal intubation.

Objective To investigate the correlated factors of prognosis for acute myeloid leukemia (AML).Methods A retrospective analysis of initial 182 patients with AML was performed to assess the relationship between prognostic factors including sex,age,white cell count at diagnosis,immunophenotype,cytogenetics,allo-HSCT and complete remission (CR) after one-course of induction and overall survival (OS) or event free survival (EFS).Results The median age of 182 patients was 49 (14-80) years old,and the median follow up was 9.7 (0.5-75.5) months.There were 107 patients achieved CR after one-course of induction (total response rate was 65.9 ％,120/182),age≥60 years.CD64 negative,unfavorable cyto-genetics and failing to achieve CR after one-course of induction were associated with lower total CR rate.Univariate analysis results demonstrated that age ≥60 years,unfavorable cytogenetics,CD11b expression and failing to achieve CR after one-course of induction had poor influence on 3-years OS and EFS,while the 3-years OS and EFS for patients with CD19 expression,CD64 expression and allo-HSCT were better than that of the patients with negative CD19 and CD64.Multivariate analysis suggested that age,cytogenetics,CD11b,CD64,allo-HSCT and CR after one-course of induction were independent prognostic factors for OS,while cytogenetics,CD64,allo-HSCT and CR after one-course of induction were important on EFS.Conclusions Prognostic analysis depending on AML corelation factors is benifit to individual therapy and can be applied to improve the survival.

Objective:To investigate the effect of EBV protein on the production of Immunoglobulin(Ig)by cultured B cells.Methods:The purified human umbilical blood B cells were treated respectively with UV and Heat-inactivated EBV and cultured in complete IMDM. CD5, CD3, CD4 and CD8 cells were measured by flow cytometric analysis. IgG and IgM in the supernatant were detected by ELISA. At the mean time, IgG and IgM in the supernatant of cultured EBV-transformed B lymphocytes were detected by ELISA,and compared with that of UV-inactivated EBV group at the same period.Results:In UV-inactivated EBV group,CD3, CD4 and CD8 T cells couldn’t be detected,CD5 + cells occupied about 42% of all detected cells at the 14th day; and at the 28th day, 47%.The IgG A value of the 10th、18th、22th and 26th day in UV-inactivated EBV group have a significant difference compared with that in the control group ( P

The current official quality control approaches meet the challenges from the complexity of herbal medicines.In fact,any herbal medicines containing numerous unknown components,its curative effect usually depends on the whole of herbal medicines,so it is impossible and unnecessary to qualitatively and quantitatively study every component.By investigating the limitations of current quality control approaches for herbal medicines and the difference and similarity in the chemical substantial style as well as quality control pattern of herbal medicines,a new quality control approach for Chinese herbal medicines should be explored and designed.The combination approach of chemical analysis with bioassay is promising to be developed and employed in order to ensure the safety and efficacy of Chinese herbal medicines.

[Objective]To investigate the value of double plates in the anterior approach for the treatment of jumping multilevel CSM.[Method]From July 2003 to July 2004,21 cases of jumping multilevel CSM and 7 cases of CSM were operated with single level discectomy/anterior corpectomy and self-iliac bone graft and fixation by double plates.During the 6,9,12 month follow-up,bone fusion were assessed and neurological function、complications were recorded.[Result]All patients were followed up for 16~32 months(24 months on average).Based on JOA evaluation,the average improving rate was 88.5%.And 82.1% fusion was achieved at 6 months,the fusion rate reached 89.3% and 93% at 9 and 12 months accordingly.There were 6 cases with complications in the donor site of grafting.Indications of this approach:the compressing pathology was located anterior to the spinal cord.This included central,broad-based disc herniations and large bridging osteophytes at or adjacent to the level of the disc space.And it was especially indicated for patients with jumping multilevel CSM who did not have a lordotic spine.Complications:complications occurring with this approach could be related to injuries to the soft tissue during dissection or to the neurological elements.In addition,postoperative fusion or hardware failure could occur.[Conclusion]Single level discectomy/anterior corpectomy and self-iliac bone graft and fixation by double plates is an effective treatment to the jumping multilevel CSM.It allows restoration of the spinal canal and neural foramen to normal dimensions to decompress the spinal cord.To the cases of jumping multilevel CSM that have a kyphotic deformity,it allows anterior decompression and reconstruction of the spine to help restore a lordotic curvature.

Objective To discuss effects of Wugong Baidu Drink on expressions of Bcl-2, NF-κB mRNA genes related to the apoptosis of HaCaT in psoriasis. Methods Wistar rats were randomly divided into 6 groups:blank group, Western medicine control group, TCM control group, and Wugong Baidu Drink high, medium, and low dose groups. They were given normal saline, methotrexate suspension, compound natural indigo capsule suspension, and the different doses of Wugong Baidu Drink for gavage, respectively. Drug-containing serum of each group was prepared, and was put in the HaCaT respectively to subculture. The intervention effect of different Drug-containing serums on expressions of objective genes were detected through PR-PCR method. Results Drug-containing serum of each group could decrease the expressions of Bcl-2 mRNA and NF-κB mRNA. The most obvious effects were shown in the groups of Western medicine control group and Wugong Baidu Drink high dose group. All Wugong Baidu Drink groups showed a dose-response relationship. Conclusion Wugong Baidu Drink probably can promote the apoptosis of HaCaT by reducing the gene expressions of Bcl-2 and NF-κB. It can also decrease immune response by regulating serum factors related to T cells to play the role of anti-psoriasis.

Objective To analyze the characters about anticoagulation EDTA on the platelet aggregation ,and discuss the risk factors about EDTA‐PTCP by analyzing the biochemical parameters .Methods 30 700 EDTA‐K3 blood samples were collected from January 2014 to August 2015 .WBC was performed using Sysmex XN‐3000 and 27cases were identified of EDTA‐PTCP .The blood samples of 27 subjects were recollected and WBC was repeated using EDTA‐K3 and sodium citrate blood ,respectively .Manual platelet counting and smear microscope examination on periphery blood were also performed .Biochemical parameters of 27 subjects with EDTA‐PTCP were compared with 50 randomly selected non EDTA‐PTCP controls .Results Platelet counts in citrated blood were significantly higher than EDTA blood ,the difference was statistically significant(P< 0 .05) ,but similar to manual platelet counting(P>0 .05) .ALT ,Glu and TG in patients with EDTA‐PTCP were significantly higher than normal people ,but HDL was significantly lower than control group ,the difference was statistically significant(P<0 .05) .Conclusion EDTA‐dependent pseudo‐thrombocytopenia varies from people with different disease or using different medicine .It may be relative to hyperglycemia and hy‐perlipidemia .Another approach for rectifying ,changing anticoagulant or manual platelet counting is a better way lead to correctly di‐agnose when EDTA‐PTCP occurred .

Type 2 diabetes mellitus is a metabolic disorder characterized by hyperglycemia caused by multifactors.The incidence of type 2 diabetes is gradually increased.Its prevention and treatment are paid more and more attention.Intestinal flora is one of the hot spots in recent years.A large number of studies have shown that intestinal flora is closely related to the occurrence and treatment of type 2 diabetes mellitus.Intestinal flora is expected to be a new direction for the improvement and treatment of type 2 diabetes mellitus.In this paper,the relationship between type 2 diabetes and intestinal microflora in recent years,pathogenesis and treatment were summarized.