Objective:To investigate the serum level of IL-34 in patients with acute phase Henoch-Schoenlein purpura ( HSP) and its potential significance .Methods:38 HSP patients were recruited ,together with 21 healthy volunteers as control .The serum level of IL-34,IL-6 and TNF-αwere determined using enzyme-linked immunosorbent assay (ELISA) or chemiluminescence.The correlation analysis was performed to explore the correlation of IL-34 with clinical parameters and the serum level of IL-6,TNF-α.Results:The se-rum levels of IL-34,IL-6 and TNF-αin HSP patients were significantly higher as compared to the control group (P<0.001).The serum level of IL-34 was positively correlated with that of high sensitive C-reactive protein ( hs-CRP) and IL-6 ( r=0.453 ,P=0.004 and r=0.469,P=0.003,respectively).Conclusion:IL-34 might be involved in the pathogenesis of HSP ,and the potential mechanisms of ac-tion is associated with the aberrant expression of pro-inflammatory factors in HSP ,which promote the development of inflammation in the vascular system.

Objective To find out the impact of SHI-1 cells on VOCs in the headspace, and to assess the feasibili-ty of VOCs analysis in leukemia patients or with other tumors in the differential diagnosis by determing VOCs in the headspace of acute mononuclear leukemia cells(SHI-1). Methods The air samples from the headspace of SHI-1, Human macrophages cells and medium control were collected by syringes, and then determined by means of solid phase microextraction-gas chromatography/mass spectrometry ( SPME-GC/MS ) in order to have a better under-standing of the concentration distributions and changes of VOCs in SHI-1 cells' headspace. Results Using Mann-Whitney U test, we found that eight kinds of VOCs were different among them. They were 2,4-dimethyl heptanes, 4-methyl octane, chloroform, benzene, 3,7-dimethyl dodecanese, hexanol, cyclohexanol, cetane. And alkane, benzene were significantly higher than the control group ( P<0.05 ) , alcohol decreased significantly ( P<0.05 ) . Conclusion SHI-1 cells have an impact on the concentration distributions of VOCs in their headspace. The most outstanding VOCs are alkanes, alcohol and benzene compared with the blank control group. They may be potential markers characteristic of leukemia cells.

Objective To discuss the clinical efficacy of warm acupuncture, acupoint injection, bloodletting and electroacupuncture, and their interaction effects for acute cold type facial paralysis. Methods Totally of 48 patients with acute cold type facial paralysis were randomly divided into three groups by orthogonal experimental design, and then were assigned to 16 treatment plans. All patients received routine basic treatment, and then were given warm acupuncture, acupoint injection, bloodletting and electroacupuncture according to assigning results. 10 d were as a treatment course, with 2 d as interval, 3 treatment courses in total. The neurological function scores before and after treatment and facial nerve recovery time were recorded. Results There was statistical significance in scores of facial nerve function, warm acupuncture, acupoint injection, electroacupuncture, bloodletting had significant difference with errorterm (P<0.01). There was statistical significance in the recovery time of the facial nerve function, warm acupuncture, bloodletting compared with error term (P<0.01). Conclusion Warm acupuncture, acupoint injection, bloodletting and electroacupuncture combine with acupuncture are better for acute cold type facial paralysis. Warm acupuncture and bloodletting therapy in the acute phase of interventional therapy can shorten the recovery time of facial nerve.

Objective To conduct the anatomical observation and measurement on adult temporal bone labyrinth and internal auditory canal structures to accumulate the anatomical data and provide anatomical basis for clinical internal ear surgery.Methods Thirty sides(left 16 and right 14)of adult dry temporal bone labyrinth and internal auditory canal structures were anatomically observed and the related structures were measured.Results (1) The specimen observation:the vestibule was egg conical cavity,and angle between long axis and sagittal plane was about 33°.The floor of internal auditory canal were divided into four districts,which angle between the inferior surface and vestibule was about 90°,the connection between tractus spiralis foraminosus bone crest of cochlea area and inguinal ring of cochlear spiral canal was the same rotated laps.(2)The data measurement:the comparison of means in various measurement items had no statistical differences between the left side and right side(P＞0.05);the comparison of mean length and crus distance had statistical differences among superior semicircular canal,posterior semicircular canal,lateral semicircular canal(P＜0.05);the comparison of means had statistical difference among posterior semicircular canal and superior semicircular canal,lateral semicircular canal(P＜0.05),but the comparison of means had no statistical difference between superior semicircular canal and lateral semicircular canal(P＞0.05).Conclusion The means of various measurement items in bone labyrinth and internal auditory canal have no difference between the left side and right side;the comparison of length means:posterior semicircular canal＞superior semicircular canal＞lateral semicircular canal;the comparison of crus distance means:superior semicircular canal＞ lateral semicircular canal＞posterior semicircular canal;the comparison of diameter means:posterior semicircular canal＞superior and lateral semicircular canal,but the diameter has no difference between superior semicircular canal and lateral semicircular canal.

28 days after injury (P < 0.05). In the fracture+spinal cord injury group, the level of serum transforming growth factor beta 1 had a rapid increase on the 7th day, and reached the peak on the 14th day, and then, this level had no significant decrease until the 28th day. In the simple fracture group, the level of serum transforming growth factor beta 1 began to increase on the 2nd day, reached the peak on the 7th day, and then decreased gradualy. Remarkable changes of serum transforming growth factor beta 1 levels in patients with bone fracture combined with spinal cord injury may be associated with fracture healing in different periods.

BACKGROUND:In the animal model of spinal cord injury associated with fractures, the trauma is severe and postoperative survival rate is low. The improved Al en method and open femoral osteotomy method for making animal model has many advantages, such as simple operation, no need of special equipment, quick establishment, shortened operation time and reduced intraoperative bleeding, so they are suitable for preparing a femoral fracture model combined with spinal cord injury.
OBJECTIVE:To design an animal model of femoral fracture combined with spinal cord injury, which can maintain long time survival, meet clinical features, and is simple and easy.
METHODS:Forty-eight male Sprague-Dawley rats were randomly divided into simple femoral fracture group and femoral fracture merging spinal cord injury group. Femoral fracture model was caused by opening osteotomy to cause transverse fracture and implantation of internal fixator in femur. According to the improved Al en method, a self-made blow device was applied to cause acute T 10 segment contusion injury of spinal cord in rats. Thus the femoral fracture model merging spinal cord injury was successful y established. The rats in two groups were grossly observed at different time points after the modeling, and the fracture healing at 4 weeks was detected.
RESULTS AND CONCLUSION:Al the animal models of femoral fracture with spinal cord injury survived, which exhibited the loss of sensory and motor function of the lower limbs, but could slowly creep forward by the upper limbs. In the first 3 days, the rats had poor appetite and few activity, with tail suspension at night there were no ischemia and necrosis of the limb fracture. At 4 weeks, one rat in simple femoral fracture group died, while four rats in femoral fracture merging spinal cord injury group died, with the survival rate of 83.33%, intramedul ary fixation were not prolapsed. In the two groups, continuous bone cal us formation was found in the fracture, and the bone cal us volume in femoral fracture merging spinal cord injury group was significantly higher than those in simple femoral fracture group. The results demonstrated that combining the improved Al en method with smal lateral incision open femoral osteotomy is a simple and feasible method for the establishment of femoral fracture model merging spinal cord injury, and the models survive for 4 weeks.

Objective To observe the effect of passive smoking on Clara cells and secretion of Clara cell secretory protein (CC16) in rat lung tissue and to find some relations between passive smoking and chronic inflammation in the lung. Methods 40 male rats were randomly divided into 4 groups: passive smoking for 1 month (group 1), 2 months (group 2), and 3 months (group 3) and control group (n=10). The change of Clara cell ultrastructure was detected through transmission electron microscope and the lung pathology was examined by HE stain. The expression of CC16 mRNA in the lung was detected by RT-PCR. The number of Clara cells and synthesis of CC16 were detected by immunohistochemistry, the CC16 level in BALF was detected by ELISA. Results The ultrastructure of Clara cells in group 3 was destroyed greatly and the formation of pneumatocele was observed. The percentage of Clara cells in terminal bronchioles in group 2 and group 3 significantly decreased than that of the control(P

Scientific research capacity of college students is the direct embodiment in innova-tive qualities. Taking Zhaoqing Medical College as an example, by guiding students anatomy innova-tion practice activities including literature review, fund grant application, research experiments and achievement paper writing, and guiding the two team works such as Challenge Cup prize at the provincial level, the author elaborates on the fact that teachers and students building team is an effec-tive way to cultivate students' innovative ability, so as to make the positive exploration for establishing innovative talents of training system in higher vocational colleges.

Objective To develop a method for the direct determination of eight life elements (Ca, Mg, K, Al, P, Cu, Zn, Fe ) in serum by ICP-AES. Methods The serum samples were determined by ICP-AES after diluted in 0.1% Triton X-100. Results The RSD of the method were 2.36%-9.46% and the recovery rates were 87.3%-116.5%. Conclusion This method was quick, simple and accuracy, which is suitable for direct determination of life elements in serum samples at one time.

Objective:To investigate the mechanism of the changes of the intestinal mucosal barrier in Severe acute pancreatitis.Methods:Twenty patients with Severe acute pancreatitis were admitted and 10 volunteers as the control group.The levels of serum tumor necrosis factor alpha(TNF_?)、nitric oxide(NO)、diamine oxidase(DAO) and the concentration of plasma endotoxin(ET) were measured.The ratio of lactulose to mannitol in urine was detected by HPLC with Pulsed Electrochemical Detection.Results:Compared with the control group,significantly increasing parameters could be seen in all the patients with Severe acute pancreatitis,including the ratio of lactulose to mannitol in urine (P