Objective:To evaluate the clinical use rationality of edaravone injection in our hospital to improve the level of rational use of drugs in clinics.Methods:Totally 500 medical records with edaravone injection from January to June in 2016 were selected randomly based on the hospital medical record management system.According to the drug package insert,relative literatures and information,the clinical use rationality was evaluated.Results:Among the 500 medical records,neurosurgery patients accounted for 48%,nerve internal medicine patients accounted for 37%,ICU patients accounted for 7%,orthopedics patients accounted for 5%,and the patients in the other departments accounted for 3%.Among the 500 medical records,108 ones involved off-label drug use,which accounted for 21.6%,180 cases involved improper use frequency,which accounted for 36%,201 cases involved improper treatment course,which accounted for 40.2%,37 cases involved improper drug administration time,which accounted for 7.4%,3 cases involved inappropriate solvent choice,which accounted for 0.6%,and 4 cases involved special population,which accounted for 0.8%.Among the 500 medical records,totally 261 (52.2%) ones were irrational.Conclusion:The overdose use and improper treatment course of edaravone injection in our hospital are severe,meanwhile,improper frequency and notice ignorance are common as well,which should be paid more attention.

Objective To investigate the correlation between serum lipoprotein associated phospholipase A2 (Lp‐PLA2) level and severity of coronary artery stenosis in the patients with coronary heart disease(CHD) and its clinical significance .Methods A total of 243 cases of CHD and suspected CHD were divided into the high‐risk group(at least 1 coronary artery vessel stenosis >50% ,n=102) and low‐risk group(single coronary artery vessel stenosis≤50% ,n=141) according to the coronary angiography re‐sults .The clinical data were collected and Lp‐PLA2 level was detected by ELISA .Results The serum Lp‐PLA2 level in the high‐risk group was significantly higher than that in the low‐risk group ,and the difference was statistically significant(P<0 .01) ,the sensitivity of Lp‐PLA2 for judging the high‐risk patients with CHD was 86 .3% ,the specificity was 96 .6% ,and the overall diagno‐sis accuracy was 98 .0% .Conclusion Lp‐PLA2 can better assess the risk of CHD patients and the serum Lp‐PLA2 level can reflect the severity of coronary artery lesion .

Cooling bag for heat stroke in wild field was invented in order to fulfill rapid cooling for heat stroke in field environment. The cooling bag is composed of the hood, the body cover (which is made up of the anterior portion, the linking, and the posterior portion), the fixed straps, the handles. The length is 200 cm, the regulative width is 60-70 cm, the folding volume is 26 cm× 20 cm× 9 cm, and the weight is 1.4 kg. There are a number of pockets for the cold sources in the hood and the body cover. Fixed straps are set in the upper, middle and lower of the bag. The handles are set in the hood and the body covers. Usage: put the activated cold sources in the pockets, then put off the patient's clothes, stretch his/her arms into the linking, zipper up and wrapped up by the fixed straps; the amount of cold sources can be adjusted or changed according to the temperature. The patient's temperature, blood pressure, intravenous infusion can be monitored during the cooling course. The handles in the hood and the body cover allow the patient to be easily moved and be transferred in rescue. The cooling bag for heat stroke in field has good cooling effect, and are reusable and easy to be carried, operate, monitor and move, with low cost, which make it popularization and application.

Objective To explore the electrophysiological examination results and risk factors of type 2 diabetic peripheral neuropathy. Methods 337 patients with type 2 diabetes from August 2014 to December 2016 in the first people's hospital in Shizuishan city were divided into DPN group (n=218) and NPDN group(n=119) according to the results of NCV and SSR examinations. The general information and laboratory biochemical indicators in the two groups were compared. Multivariate Logistic regression was used to analyze the risk factors of DPN. Results The diagnosis rate of DPN detected by NCV combined with SSR was higher than that of NCV or SSR alone(P<0.05);There were significant differences in age,duration of diabetes,history of hypertension,systolic blood pressure,2h FBG,HbA1c,FINS,2 h INS,FC-P, 2h FC-P,ACR between the DPN group and NPDN group(P<0.05);Logistic multivariable analysis showed that age, duration of diabetes, 2h FBG, HbA1c, ACR were independent risk factors for DPN. Conclusion It is beneficial to increase the diagnosis rate of DPN by NCV combined with SSR. There is a higher incidence rate of DPN type 2 diabetes patients with older grade, longer duration of diabetes, higher 2h FBG, HbA1c and ACR.

Objective To investigate the significance of combined detection of homocysteine(Hcy) and ischemia modified albu‐min(IMA) in serum to the diagnosis of acute myocardial infarction(AMI) .Methods The serum levels of Hcy and IMA were detec‐ted in 80 patients with acute myocardial infarction ,60 patients with angina pectoris and 40 healthy subjects(control group) ,and the results were statistically analyzed .Results The concentration of Hcy and IMA in AMI patients was significantly higher than which in the control group ,the difference is statistically significant(P<0 .05);the sensitivity of the Hcy+ IMA combined diagnosis was higher than that of the single project .Hcy and IMA was positively related(P<0 .05) .Conclusion The combined detection of Hcy and IMA levels in patients with acute myocardial infarction has a good effect on early diagnosis and monitoring ,and it is worth popularizing .

Objective:To explore the pharmaceutical care model for the patients with acute lung injury/acute respiratory distress syndrome ( ALI/ARDS) caused by inhalation of chemical agents to ensure the safety, rationality and effectiveness of drugs. Methods:According to the characteristics of drug therapy for the patients with ALI/ARDS caused by inhalation of chemical agents, the pharma-ceutical care was carried out and the suggestions on the medication were given. Results:Through the pharmaceutical care, the safety, reasonability and effectiveness were improved. And the incidence of adverse drug reactions was decreased. Conclusion: Cooperating with clinical physician, clinical pharmacists can perform active pharmaceutical service and optimize dosage regimen, which is beneficial to the safety, reasonability and effectiveness of drug therapy for the patients.

A new type of fluorescent gold nanoclusters (MU-Au NCs) was prepared by hydrothermal synthesis method using ammonium benzoate murexide (MU) as reducing agent and protecting agent.The synthesis method was simple and rapid.Based on the fluorescence quenching ability of spermine, a turn off type fluorescence analysis method was established for rapid and ultra sensitive detection of spermine.The linear range for detection of spermine was 0.003-300 μmol/L and the detection limit was 1 nmol/L (S/N=3).The established analytical method of spermine provided theoretical basis and reference for construction of spermine biosensor and actual sample detection.

BACKGROUND: Based on the multi-directional differentiation potential,the combined application of proper biological materials and growth factor can repair tissue defect.OBJECTIVE: To observe the growth characteristics of rabbit bone marrow-derived mesenchymal stem cells and their osteogenic potential under the induced condition.DESIGN: A single sample experiment.SETTING: The Department of Histology and Embryology of Youjiang Medical College for Nationalities; the Affiliated Hospital of Youjiang Medical College for Nationalities.MATERIALS: This experiment was conducted at the Department of Histology and Embryology of Youjiang Medical College for Nationalities in June 2004. Male new Zealand rabbits, with a body mass of 2.0 to 2.5 kg,were provided.METHODS: Rabbit bone marrow tissue was extracted. After gradient centrifugation, adherent cells were kept for cell passage. Culture medium was used for inducing culture of osteoblast when the passage was stable (50 mL/L of Dulbecco modified culture medium of bovine serum containing 10 mmol/L of β-sodium glycerophosphate, 10 nmol/L of dexamethasone, 50 mg/L of vitamine C). The culture medium was changed every other day. Osteogenic characteristics of the bone marrow-derived mensenchymal stem cells were observed under an inverted microscope after alkaline phosphatase immunohistological staining, osteonectin and osteopontin immunocytochemical staining.MAIN OUTCOME MEASURES: ① Morphological observation of bone marrow-derived mesenchymal stem cells; ② Osteogenic characteristics of bone marrow-derived mesenchymal stem cells.RESULTS: ① Morphological observation of bone marrow-derived mensenchymal stem cells: Primary rabbit bone marrow-derived mesenchymal stem cells could cover the Petri dish fully in 7 or8 days and could passage stably. Generative cells began to passage in 5 or 6 days. ② Osteogenic characteristics of bone marrow-derived mensenchymal stem cells:Alkaline phosphatase immunohistochemical staining showed a lot of brown particles in the kytoplasm, but no colored cells were found in the control group. Osteonectin and osteopontin immunohistochemical detection found that the nucleus was light blue and a lot of brown particles appeared in the kytoplasm, presenting obvious strong positive, but no brown particles appeared in the control group.CONCLUSION: After combined induced culture with dexamethasone, vitamine C and β-sodiumglycerophosphate, rabbit bone marrow mensenchymal stem cells showed the morphological and biological characteristics of osteoblasts with osteoblastic activity, and were able to supply autologous seed cells for constructing in vitro tissue engineered bone in a short period of time.

Objective To use tri-operator breast blood oxygen imaging in diagnosis of early stage breast cancer.Methods To analysis and diagnosis eighty cases with the technology of breast blood oxygen imaging.Results The accurate rate of the diagnosis made by technology of breast blood oxygen imaging was 93.75%,the sensitivity of diagnosis was 90.63%,the specificity was 95.83%.Conclusions The technology of breast blood oxygen imaging without the radiation may be a better methods to diagnosis the breast diseases,which has the higher sensitivity than infrared rays examination on breast cancer diagnosis.

Objectives: To analyze the status quo and trends on the burden of cerebrovascular diseases between 1990 and 2016 in China. Methods: Morbidity mortality, years of life lost (YLL), years of lived with disability (YLD) and disability-adjusted life year (DALY) related to cerebrovascular diseases between 1990 and 2016, were collated and analyzed, according to the results of the Global Burden of Diseases Study 2016 (GBD 2016). Numbers on incidence and morbidity were used to assess the incidence of diseases, while the numbers of death and mortality were used to assess the death of diseases. Years of life lost due to premature death (YLL), years lost due to disability (YLD) and disability-adjusted life year (DALY) were used to assess the burden of diseases. Changing trend on the burden of cerebrovascular disease from 1990 to 2016 was also analyzed. Results: In 2016 and 1990, the numbers of new cases/morbidity and the number of deaths/mortality on cerebrovascular diseases in the country showed an upward trend. Rates regarding YLL and DALY on cerebrovascular diseases remained stable from 1990 to 2016, however, the YLD rate showed a slow upward trend. The changing rate of DALY was mainly influenced by YLL. Both DALY and YLL crude rates in males showed a slow upward trend, with the highest DALY rate appearing in the ≥70 age group. Disease burden on males was heavier than that of the females and in the 50-60 age group, which taking the largest proportion. As for the composition in DALY, YLL appearing much larger than YLD and slowly increasing. Analysis on the subtypes of diseases, proportions of YLL and DALY in hemorrhagic stroke were greater than that in ischemic stroke while the proportion of YLD in ischemic stroke was in the opposite. Conclusions The burden of disease on cerebrovascular diseases remained heavy and the differences appeared in age, gender and subtypes of diseases. Our findings called for the adoption of measures including screening, intervention and rehabilitation to be taken on target populations, in order to reduce the burden on both individuals and the society.