Objective To develop the scale on work values for college students. Methods 63 items were determined through reviewing items in related literatures and scales, experts consulting and panel discussion.528 students from 3 universities in Shanghai were enrolled in the study. The data were analyzed through exploratory factor analysis,discrimination analysis,reliability analysis and other statistical analysis methods. Results College students' work values comprises six dimensions: self-value dimension ( including two sub-dimensions: self-esteem dimension and self-realization dimension), social-value dimension, dimension of the working environment, wages and welfare dimension , job stability dimension, and the dimension of social status. 43 items were reserved after exploratory factor analyses. The coefficient of reliability of the 43 items was 0.943, and the item loading of the 43 items was 0. 321 ～ 0. 792. Conclusion The discernment, internal consistency and validity of the scale accord with psychometric demands.

Objective To study the differences in serum antioxidant ion status of adults living in different regions and relat-ed influential factors.Methods Serum levels of total antioxidation capacity(TAOC),superoxide dismutase(SOD)and malon-dialdehyde(MDA)were determined in37local residents in Xinjiang(Xinjiang group)and37Tianjin residents(Tianjin group).Results Serum levels of TAOC and SOD were significantly higher in the Xinjiang group than that in the Tianjin group(P0.05)for the MDA between the two groups.The TAOC levels were sig-nificantly higher in the females than that in the males(P

PUMA (p53 up-regulated modulator of apoptosis) is a recently discovered Bcl-2 family member which could be rapidly induced by p53 and has strong pro-apoptotic effects.PUMA has attracted much attention in the research of life science.PUMA expression results in potent growth suppression of some cancer cells through induction of apoptosis.PUMA can also significantly sensitize some cancer cells to chemotherapeutic agents and irradiation through induction of apoptosis.PUMA is potentially useful in gene therapy of tumor.But recently,researchers have also found that PUMA participates in the process of carcinogenesis and possessed important biological functions.

ObjectiveTo assess the relationship between Candida adhesivity and biofilm formation. MethodsEight Candida strains belonging to 8 species and 1 Saccharomyces cerevisiae strain were cultured in yeast peptone dextrose (YPD) fluid and agar medium respectively. The flocculation and adhesion of Candida were observed. Candida biofilm models were developed in 96-well microculture plates. The kinetics of biofilm formation was measured. ResultsAll the 9 fungal strains had flocculation capability and could adhere to the surface of the yeast peptone dextrose agar medium. After mild shaking of the fluid medium, it is difficult for C. albicans, C. kefyr, C. glabrata and C. tropicalis to resuspend, but easy for Saccharomyces cerevisiae. The adhesivity of C. albicans, C. kefyr, C. glabrata and C. tropicalis was stronger than that of the other Candida strains. Common pathogenic Candida strains could form biofilm, and the metabolic activity of Candida cells in the biofilm increased over time. A significant increment was observed in the ability of C. albicans and C. kefyr to form biofilm compared with the other species(all P ＜ 0.05), and in that of C. tropicalis and C. glabrata compared with C. krusei, C. parapsilosis and C. gulliermondii (all P ＜ 0.05). The nonpathogenic Saccharomyces cerevisiae could not form biofilm. ConclusionsCandida has the ability to adhere and form biofilm,and the ability varies with Candida species. Moreover, the ability to form biofilm positively correlates with the adhesivity of Candida.

Objective To investigate the effects of early continuous renal replacement thempy(CRRT)on acute lung injury and prognosis in severe acute pancreatitis (SAP)patients.Methods 46 SAP patients were divided into the two groups randomly:the control group and CRRT treatment group.The levels of IL-1β,IL-6,TNF-α,the APACHEⅡscore,oxygenation index,the incidence of acute lung injury(ALI)/acute respiratory distress syndrome (ARDS),ICU stay were compared between the two groups.Results The level of interleukin-6(IL-6)in the con-trol group was significantly higher than that in CRRT group in day 1(t=2.265,P<0.05);The levels of interleukin-1β(IL-1β),interleukin-6 (IL-6 ),tumor necrosis factor-α(TNF -α)in the control group were significantly higher than that in CRRT group in day 3(t=2.305,2.471,2.293,all P<0.05);the oxygenation index in the control group was significantly lower than that in CRRT group in day 3(t=2.386,P<0.05);the incidence of ALI/ARDS, the ICU stay days,the fatality rate in the control group were significantly higher than that in CRRT group(P<0.05);there was no significant differences between late group than that early group in gender,age,APACHE Ⅱ score (all P>0.05).Conclusion Early CRRT therapy can eliminate the IL-1β,IL-6 and TNF-αin SAP patients,which can improve the oxygenation index and reduce the incidences of ALI/ARDS,may provide more clinical benefits in the early phase of SAP.

AIM: To investigate the effect of compound rhubarb preparation (Kintop) in preventing obesity in rats and its probable mechanism involved. METHODS: Twenty-six newborn SD rats were randomly grouped as rhubarb preparation plus high-energy forage group( n= 8), high-energy forage control group( n= 8) and ordinary forage control group( n= 10). The rats in rhubarb preparation plus high-energy forage group and high-energy forage control group were fed with high-energy forage and those in ordinary forage control group were fed with ordinary forage. The rats in rhubarb preparation plus high-energy forage group were administered by compound rhubarb preparation (40 mg?100 g -1 body weight?d -1 ) from 9th to 17th week. The dynamic changes in body weight, celiac fat weight and adipocytes size were measured. Immunohistochemical analysis of leptin in celiac adipocytes (ABC method) and measurement of serum leptin level (RID method) were performed. RESULTS: The body weight and the wet weights of celiac fat were lower, their adipocytes were smaller and immunohistochemical stainings of leptin were weaker in rhubarb preparation plus high-energy forage group than those in high-energy forage control group. There was an obvious positive correlation between the expression of leptin and celiac fat tissue weight( r= 0.8663, P

Objective To investigate the clinical value of the echocardiography in the diagnosis of congenital mitral valve malformation and summarize its occurrence and type associated with other cardiac malformations.Methods The echocardiographic image data of 101 patients with congenital mitral valve malformation were retrospectively analyzed,the characteristics of different types were summarized and compared with operation results,CT,cardiac catheterization,transesophageal echocardiography,as well as the data of echocardiographic follow-up.Results Among 101 patients with echocardiographic examination showed mitral valve congenital malformation,63 cases were isolated valve set malformation (62.4％),and multiple valve set malformation were found in 38 cases (32.7％).In another side,39 cases were of single mitral valve malformation (38.6％),while 62 patients combined with other type cardiac malformations (61.4％).No significant difference of dynamic change was shown between patients with solitary and multiple malformation of mitral valve (P ＞0.05).Conclusions Echocardiography can display congenital mitral valve malformation in a fast,robust,real-time way.It has an important clinical value in the diagnosis of congenital mitral valve malformation.

A rapid headspace gas chromatography tandem mass spectrometric ( HS-GC/MS ) method was established for the analysis of volatile fatty acids ( VFAs ) in the feces. Feces were suspended by 6%phosphoric acid aqueous solution (1:2 m/V) and sealed in the headspace bottle for HS-GC/MS analysis. The HS-GC/MS method was optimized as follows: agitator temperature ( temp. ):80 ℃, syringe temp.:80 ℃, sample incubation time: 30 min, injection: 1 mL without split-flow. The chromatographic separation was performed on a DB-FFAP capillary column (30m×0. 25 mm×0. 25 μm) with injection port temp.:250 ℃. The temperature program ( initial temp. at 50 ℃ within first 1 min, and raised to 200 ℃ by 10 ℃/min) was employed by fixing the flow of carrier gas (high purity helium) at 1. 0 mL/min. The electron energy at -70 eV for electron impact ( EI ) ionization, ion source temp.: 250 ℃, transfer line temp.:280 ℃, the voltage of electron multiplier at 0. 95 kV. The spectra were recorded in the range of m/z 33-200 for full scan. The established HS-GC/MS method could be applied to analyze VGAs in the feces from human and rat appropriately. There are nine VFAs identified in the feces from human, and eight VFAs detected in the feces from rat by retrieving the NIST library, comparing with the standards and analyzing the MS data. Furthermore, the relative percentage contents of acetic acid, propionic acid and butyric acid accounted for roughly 85% of all VFAs by area normalization. The method is simple and sensitive, and it can be used to rapidly detect VFAs in the feces from human and rat.

ObjectiveTo investigate the relationship between metastasis-associated gene 1 ( MTA1 )expression and invasive and metastatic ability of cervical cancer cell. MethodsThree kinds of plasmids pcDNA3( control group), pcDNA3-MTA1 ( MTA1 group) and pSilencer3. 1-MTA1-siRNA ( MTA1-siRNAgroup) were transfected into human cervical cancer cell line CaSki cells. Reverse transcription (RT)-PCR and western blot were used to detected MTA1 mRNA and protein expressions. The effects of MTA1 expression on CaSki cell growth and proliferation, cell migration, adhesion and invasion, and cell cycles were tested by methyl thiazolyl tetrazolium (MTT), clone formation experiment, wound-healing assay, transwell assay, adhesion assay and flow cytometry, respectively. In animal experiment, three groups of cells were inoculated to BALB/c nude mouse subcutaneously to observe tumor formation ability. ResultsCompared with control group, MTA1 mRNA and protein were significantly overexpressed in MTA1 group, while MTA1-siRNA group showed lower MTA1 expression. Compared with control group, MTA1 group showed significantly accelerated cell growth; while MTA1-siRNA group showed decreased cell growth since the second day (P＜0. 05). Clone formation number in control, MTA1 and MTA1-siRNA group were 133 ±6, 169 ± 10 and 57 ±5,respectively. MTA1 group showed accelerated cell formation, while MTA1-siRNA group showed the reverse effect compared with that in control group(P ＜ 0. 05 ). At 24, 48 and 72 hours after wounding, the healing ability of MTA1-siRNA group significantly lagged behind that in the control group, while MTA1 group showed accelerated cell healing ability. The adhesion rate of control, MTA1 and MTA1-siRNA group were (69. 3 ± 3. 6) ％, ( 80. 4 ± 5. 6 ) ％ and ( 39. 2 ± 7.4 ) ％ separately at 90 minutes after cell seeding. In contrast with control group, MTA1 group promoted the adhesion of CaSki cell to matrigel matrix, while MTA1-siRNA group inhibited the adhesion process (P ＜0. 05 ). In the migration assay, the number of cells migrated to the bottom side of the membrane in control,MTA1 and MTA1-siRNA group were 153 ± 17,247 ± 38 and 82 ± 10, respectively. The number of cells in the invasion assay were 231 ± 19,354 ± 36 and 76 ± 7, respectively. Compared with the control group, MTA1 group significantly increased the migration and invasion ability, while MTA 1-siRNA group showed lower cell migration and invasion ability (P ＜ 0. 05 ). In cell cycle experiment, no significant differences of cell proportions including G1, S and G2 stage were found among three groups (P ＞ 0.05).In animal experiment, compared with control group,MTA1 group showed accelersted tumor formation and growth,whilethe MTA1-siRNA group showed the reverse effect ( P ＜ 0. 05 ). ConclusionsMTA1 may play its roles to promote cervical cancer cell invasion, migration, adhesion, as well as cell growth and colony formation, while RNA interference against MTA1 may decrease the malignant phenotypes. This study shows that it will be an effective beginning to explore metastasis mechanisms and cancer gene therapy strategy targeting MTA1 in cervical cancer.

Objective To investigate the clinical results of two minimally invasive approaches for calcaneal fractures.Methods The data of 93 patients with displaced intra-articular calcaneal fractures was retrospectively analyzed who were treated from April 2010 to September 2010 in our trauma centre.The patients were divided into two groups according to different minimally invasive approaches.Group A consisted of 50 patients (51 feet) who were treated with the minimally invasive longitudinal approach,including 47 males and 3 females with an average age of 38.22 years.Type Sanders II included 27 feet,type Ⅲ included 18 feet and type Ⅳ included 6 feet.B(o)hler angle was-1.1° on average before operation.Group B consisted of 43 patients (45 feet) who were treated with the sinus tarsi approach,including 42 males and 1 female with an average age of 41.40 years.Type Sanders II included 23 feet,type Ⅲ included 17 feet and type Ⅳ included 5 feet.B(o)hler angle was 2.6° on average before operation.The difference was investigated between the two groups in their operation time,complications and the scores according to the ankle and hindfoot score system of American Orthopaedic Foot and Ankle Society (AOFAS).Results All patients were followed up in the two groups.The average time of follow-up was 28.2 months in Group A and 27.6 months in Group B.The mean operation time was 40.78 mins in Group A and 60.67 mins in Group B.The rate of complications in Group A was 3.92％ (2/51),and 8.89％ (4/45) in Group B.The mean AOFAS score was 85.96 in Group A and 88.60 in Group B.Conclusion No statistically significant difference was found between the mininally invasive longitudinal approach and the sinus tarsi approach in the effect of the treatment for the displaced intra-articular calcaneal fractures.However,the minimally invasive longitudinal approach could be a better choice for its shorter operation time and lower rate of complications than the sinus tarsi approach.