Objective To investigate the inhibitory effect of Pingliukang (a prescription) medicated serum on the proliferation of cultured rat C6 glioma cells and influence on cell cycle in vitro. Methods MTT colorimetry were performed to measure the levels of the proliferation of rat C6 glioma cells cultured with 2.5%, 5%, 10% and 20% of Pingliukang medicated serum for 24 h, 48 h and 72 h in vitro. The effect of Pingliukang medicated serum on cell cycle were observed by FCM. Results The proliferation of C6 cell was obviously inhibited by Pingliukang medicated serum with dose-effect relationship. The inhibitory effect of 20% of medicated serum was the strongest. When the C6 glioma cells were treated with 10% and 20% medicated serum for 48 h and 72 h, the cells of S period declined. Conclusion The Pingliukang medicated serum can inhibit the proliferation and block cell cycle of cultured C6 glioma cells.

Objective To investigate the distribution of human papillomavirus ( HPV ) in the diseased areas cut from HPV?positive cervical adenocarcinoma ( ADC ) detected by laser capture microdissection ( LCM ) . Methods Paraffin?embedded specimens diagnosed as ADC between 2005 and 2010 were collected from 9 hospitals in 7 regions across China. HPV genotyping was conducted on paraffin sections using sandwich technique and LCM in order to identify HPV infection in the tumor tissues. HE and p16 immunohistochemistry staining were performed to make histological diagnosis. Results A total of 169 cervical adenocarcinoma cases were recruited, including 94 cases of mucinous adenocarcinoma ( ADC?CX) , 9 cases of adenosquamous carcinoma ( ASC) , 19 cases of minimal deviation adenocarcinoma ( ADC?MIN) , 14 cases of clear cell adenocarcinoma ( ADC?CC) , 8 cases of endometrioid adenocarcinoma ( ADC?ENDO) , 9 cases of serous adenocarcinoma ( ADC?SER ) and 16 cases of adenocarcinoma not otherwise specified (ADC?NOS). Fourteen types of high risk HPV were detected in the whole tissue section (WTS). HPV16 was the most common type, and the second was HPV18 and HPV52, respectively. Compared with WTS, the HPV?positive rate detected by LCM was lower. The HPV positive rates were significantly different among different subtypes of cervical adenocarcinoma ( P<0.001) . After LCM, the HPV positive rate was 50.8% and 66.7% in the single infection and multiple, infection groups respectively (P=0.14). The positive rates of p16 was significantly different among different subtypes of cervical adenocarcinoma ( P<0.001) . p16?positive rate was 73. 9% in the HPV?positive samples after LCM, significantly higher than the 38. 5% of negative samples ( P<0.001) . Conclusions Laser capture dissection technique can more precisely reflect the HPV distribution in cervical adenocarcinomas. The etiological association between HPV infection and cervical adenocarcinoma occurrence is not as close as that reported in the literature.

Objective To investigate the distribution of human papillomavirus ( HPV ) in the diseased areas cut from HPV?positive cervical adenocarcinoma ( ADC ) detected by laser capture microdissection ( LCM ) . Methods Paraffin?embedded specimens diagnosed as ADC between 2005 and 2010 were collected from 9 hospitals in 7 regions across China. HPV genotyping was conducted on paraffin sections using sandwich technique and LCM in order to identify HPV infection in the tumor tissues. HE and p16 immunohistochemistry staining were performed to make histological diagnosis. Results A total of 169 cervical adenocarcinoma cases were recruited, including 94 cases of mucinous adenocarcinoma ( ADC?CX) , 9 cases of adenosquamous carcinoma ( ASC) , 19 cases of minimal deviation adenocarcinoma ( ADC?MIN) , 14 cases of clear cell adenocarcinoma ( ADC?CC) , 8 cases of endometrioid adenocarcinoma ( ADC?ENDO) , 9 cases of serous adenocarcinoma ( ADC?SER ) and 16 cases of adenocarcinoma not otherwise specified (ADC?NOS). Fourteen types of high risk HPV were detected in the whole tissue section (WTS). HPV16 was the most common type, and the second was HPV18 and HPV52, respectively. Compared with WTS, the HPV?positive rate detected by LCM was lower. The HPV positive rates were significantly different among different subtypes of cervical adenocarcinoma ( P<0.001) . After LCM, the HPV positive rate was 50.8% and 66.7% in the single infection and multiple, infection groups respectively (P=0.14). The positive rates of p16 was significantly different among different subtypes of cervical adenocarcinoma ( P<0.001) . p16?positive rate was 73. 9% in the HPV?positive samples after LCM, significantly higher than the 38. 5% of negative samples ( P<0.001) . Conclusions Laser capture dissection technique can more precisely reflect the HPV distribution in cervical adenocarcinomas. The etiological association between HPV infection and cervical adenocarcinoma occurrence is not as close as that reported in the literature.