OBJECTIVE:To discuss the effects of Kangshuling gel on wound treatment and infection prevention inⅡdegree burns. METHODS:60 patients with Ⅱ degree burns were randomized intro observation group and control group,with 30 cases in each group. Based on routine treatment (cleaning,disinfection,necrotic tissue removal,antibiotics use),observation group was given Kangshuling gel,and control group was treated with sulfadiazine. The average healing rate,healing time,surgical dressing exudate,inflammatory reaction around wound,the times of dressing change and inner layer gauze change,and wound infection rate were observed in 2 groups,and satisfaction survey was conducted in 2 groups. RESULTS:On 14th and 18th day after treat-ment,average rate of wound healing in observation group was significantly higher than in control group;the time of wound heal-ing was significantly shorter than control group,with statistical significance(P<0.05). Within 2 weeks after treatment,the number of gauze layer wetted by surgical dressing in observation group was significantly less than in control group,and case number of in-flammatory reaction around wound was significantly less than control,with statistical significance(P<0.05). The times of dressing change and inner layer gauze change in observation group was significantly less than in control group,and the rate of wound infec-tion was significantly less than control group,with statistical significance(P<0.05);the satisfaction of observation group was bet-ter than that of control group,with statistical significance(P<0.05). CONCLUSIONS:Kangshuling gel in the treatment of Ⅱ de-gree burns can effectively prevent and treat wound infection,reduce the rate of wound infection and the times of dressing change, shorten the time of wound healing and improve the rate of wound healing so,high satisfaction degree is obtained.

Objective To determine the role of valproate in the expression of SCN3A and provide new evidence for the clinical treatment of epilepsy. Methods We treated the N1E-115 cells with sodium valproate and examine the expression of SCN3A mRNA. We also constructed the expression vector of the SCN3A gene promoter then transfected the vector into N1E-115 cells ,respectively detected the level of SCN3A mRNA and the expression level of firefly luciferase of N1E-115 cells with valproate treated and untreated. Results Valproate inhibited the expression of SCN3A significantly including the level of SCN3A mRNA and luciferase protein. Conclusion Valproate inhibits the seizures by reducing the expression of the SCN3A, which provides a new understanding of the treatment of epilepsy.

Objective:To establish an HPLC method for the simultaneous determination of minoxidil and tretinoin in compound minoxidil gel. Methods:A Shim-Pack VP-ODS C18(250 mm × 4. 6 mm,5 μm)column was used with the mobile phase of methanol and 2% acetic acid solution by gradient eleution . The detection wavelength was respectively set at 280 nm and 350 nm,and the flow rate was 1. 0 ml·min -1 . The column temperature was 35℃ and the injection volume was 10 μl. Results:Minoxidil and tretinoin had a good linear relationship within the range of 4. 0- 240. 0 μg·ml-1 and 0. 05- 3. 00 μg·ml-1(r =0. 999 6 and 0. 999 3),respectively. The average recovery was 99. 56%(RSD = 0. 42% ,n = 9)and 99. 36%(RSD = 0. 50% , n = 9),respectively. Conclusion:The established method is simple,and suitable for the simultaneous determination of the two constituents in compound minoxidil gel.

Objective To explore the married women of childbearing age education on HIV/AIDS intervention method, curb the spread of HIV.Methods Use family planning check B to exceed three op-portunity, for married women of childbearing age to carry out the publicity and consultation, and design a u-nified questionnaire, respectively in the survey of 500 were statistically analyzed before and after the inter-vention, effect evaluation in description epidemiological methods.Results Before and after the interven-tion, the AIDS knowledge awareness rate of married women of childbearing age was 62.75%(2183/3479) , 97.36%(3360/3451),attitudes to AIDS positive response rate was 65.19%(324/497), 95.94%(473/493), middle school students knowledge of AIDS awareness rate was 90.48% (5130/5670), 96.99%(5110/4956 ) , the villagers'awareness rate of AIDS related knowledge was 69.61% ( 2183/3136 ) , 97.36% (3360/3451) .After the intervention, the AIDS knowledge awareness and attitude to HIV positive answer rate of married women of childbearing age were significantly improved,middle school students, vil-lagers AIDS awareness rate were significantly higher than those before intervention.Conclusions The use of B-three examinations opportunities for married women in AIDS prevention knowledge of educational inter-vention improves the life skills of women of childbearing age to prevent AIDS, and to take advantage of the core and the influence of women of childbearing age in the family will spread AIDS prevention knowledge, radiation to family members and the community to improve AIDS prevention capacity of the whole society, to curb the AIDS epidemic.

Objective To investigate the protective effect of isoflurane preconditioning on the brain against ischemia-reperfusion (I/R) injury and the underlying mechanism.Methods Seventy-five male gerbils weighing 60-80 g were randomly divided into 5 groups ( n = 15 each): group A sham operation (sham) ; group B I/R; group C isoflurane + I/R (ISO); group D 5-HD (mito-KATP channel blocker) + I/R (5-HD) and group E 5-HD + isoflurane + I/R (5-HD+ISO). The animals were anesthetized with intraperitoneal (i.p.) 10% chloral hydrate 0.35 g?kg-1 . Global cerebral I/R was produced by occlusion of bilateral common carotid arteries for 5 min and confirmed by isoelectric potential on EEG. In ISO group the animals inhaled 1.2%-1.5% isoflurane for 30 min followed by 30 min wash-out period before I/R. In 5-HD group 5-HD 10 mg?kg-1 was injected i.p. 30 min before I/R. In 5-HD+ ISO group 30 min after i.p. 5-HD 10 mg?kg-1 , isoflurane was inhaled as in ISO group. The animals were killed 24 h after global cerebral ischemia and their forebrains were excised for (1) electron microscopic examination of mitochondria and (2) measurement of mitochondrial permeability transition pore (MPTP) (ultraviolet spectrophotometer) and (3) calcium content in mitochondria (spectrophoto-fluorometer) . Results Marked swelling of mitochondria with disrupted cristae and damaged matrix were observed in group I/R (B) , 5-HD (D) and 5-HD + ISO (E) while in sham (A) and ISO (C) groups relative intact mitochondria were seen. The mitochondrial Ca2+ concentration was significantly higher and MPTP more open in I/R (B), 5-HD (D) and 5-HD+ ISO (E) groups than in sham (A) and ISO (C) groups (P

Purpose:To evaluate the immediate therapeutic effects and toxicities of intraperitoneal versus intravenous chemotherapy in advanced gastric cancer.Methods:Sixty one patients were treated with intravenous chemotherapy of leucovorin (CF) , fluorouracil ( 5 Fu) etoposide (Vp16) and intraperitoneal chemotherapy of cis platinum (DDP) in advanced gastric cancer. The short term effects and toxicities were observed.Results:The overall response rate was 57.4% and the complete response rate was 9.8% of the 61 patients .Leukopenia was observed in 95.1%, thrombocy topentia were observed in 27.8% of patients . Other side effects were uncommon.Conclusions:Intravenous chemotherapy of ELF and intraperitoneal chemotherapy of DDP were effective for advanced gastric cancer and are worthy to be further studied.

Objective:To investigate the specific immune act ivated effects in the mouse immunized by MHC I-restriced tumor antigen peptide pulsed dendritic cells(DC) with IL-2 gene modified.Methods:DC were transfected with IL-2 gene via adenovirus vector(DC-IL-2) the mRNA of mIL-2 was examined by RT-PCR.Syngeneic mice were immunized by DC-IL-2 pul sed with MHC-I-restricted Mutl tumor antigen peptiede of 3LL Lewis lung carcin oma (DC-IL-2-Mutl),the effects of lymphocyte subsets in the draining lymphoid node from the tumor antigen pulsed DCs with gene modified immunized mice by flo w-cytometric analysis(FACS) analysis.Results:The mRNA of mIL-2 was expressed in the DCs.The ratio of CD8+T cells was hosted in the dra ining lymphoid node from Mutl pulsed DCs(DC-Mutl) immunized mice.The ratio of C D8+T cells and NK cells were all hosted obviously in the draining lymphoid nod e from DC-IL-2-Mutl immunized mice .Conclusion:Immunized mice with DC-IL-2-Mutl can induce the mouse specific antitumor immunized affect in vivo and can activate a variety of immunized effect in the mouse.

Background and purpose:Hepatocellular carcinoma (HCC) is a common malignant tumor of the digestive system in our country, with high fatality, development of HCC and the machine system research and treatment is a primary issue in current study of HCC. To explore the expression ofβ-catenin at different stages in the process of hepatocellular carcinoma carcinogenisis for SD rats induced by chemicals. Methods: The experimental group included 48 male SD rats mice with primary liver cancer induced by diethylnirtosamine/carbon tetrachloride/Ethanol, while 48 normal male SD rats mice were used as the control group. The rats were killed every 3 weeks to collect the specimens and observe the pathological changes by HE staining. The changes ofβ-catenin protein expressions were detected by immunohistochemistry and Western blot respectively. Results:SD rats liver cancer was conifrmed by HE staining after 21 weeks DEN/CCl4/Ethanol induction. Immunohistochemistry showed thatβ-catenin expression level was obviously higher in the experimental group(0.27±0.01) than that of the control group(0.21±0.02) after 3 weeks induction(P<0.05). As time progresses, the expression levels ofβ-catenin kept on rising, and at the 18th(0.30±0.02) and 21th weeks(0.32±0.02), it was significantly higher than that of the earlier liver tissues of the experimental group(P<0.05), Western blot consistent with immunohistochemical results. Conclusion:β-catenin protein expression is different in the normal liver tissue, cirrhosis, liver cancer,β-catenin and the occurrence of liver carcinoma development had close relationship.β-catenin protein in the cell with further accumulation, may active a series of target gene, leading to the formation of liver cancer..

Objective To explore the effects ofBuzhong Yiqi Pills on HBV-related decompensated liver cirrhosis patients.Methods A total of 176 patients of HBV-related decompensated liver cirrhosis were enrolled in the study from January 2007 to January 2010 and were divided into treatment group (82 cases) and control group (94 cases) according to patient's wishes. Patients in both groups were given antiviral therapy. According to the liver function and complications, patients were given glycyrrhizin to protect liver, Kuhuang Injection to treat jaundice, and spironolactone and furosemide for diuretic treatment. Patients in the treatment group receivedBuzhong Yiqi Pills, one bag for each time, twice a day, four weeks as a treatment session, three sessions each year, with five-year follow-up. Effects ofBuzhong Yiqi Pills on the hepatorenal function, blood coagulation, blood routine, complications and survival rate in patients with decompensated liver cirrhosis were observed.ResultsBuzhong Yiqi Pills could effectively improve the hepatorenal function, blood routine and coagulation disorders of HBV-related decompensated liver cirrhosis patients (P<0.05,P<0.01). The rate of complications with hydrothorax and ascites (46.34% vs. 88.30%), upper gastrointestinal hemorrhage (39.02% vs. 69.15%), infection (31.71% vs. 57.45%), hepatic encephalopathy (23.17% vs. 54.26%), hepatorenal syndrome (6.10% vs. 18.09%) and chronic hepatic failure (9.76% vs. 25.53%) in the treatment group and the control group were with statistical significance (P<0.05,P<0.01). The five-year survival rates were significantly higher in the treatment group (79.27%) compared with the control group (64.89%), with statistical significance (χ2=5.353,P=0.021).ConclusionLong term use ofBuzhong Yiqi Pills can significantly decrease the complications of HBV-related decompensated cirrhosis and improve survival rate of patients.

Objective To establish a novel method to determine the absolute content of quercetin by proton nuclear magnetic resonance (qNMR).Methods DMSO-d6 was employed as solvent,and maleic acid as an internal standard.Proton signal peaks at δ7.50-7.58 and δ6.26 of maleic acid were served as quantification peaks.The content of quercetin is determined with qNMR in comparison with the results obtained by mass balance method.Results Linear regression of quantitative peak areas ratio (As/Ar) of quercetin-maleic acid vs mass ratio (ms/mr) yielded a correlation coefficient of 0.999 3 and a regression equation ofy =2.963 x + 0.134 1.The contents of three batches quercetin were 85.20％,84.93％,and 85.27％,the average was 85.13％ and its RSD was 0.21％.The results were generally consistent with that of mass balance methods.Conclusion This method was easy and simple to handle,and the analysis results were accurate.It could be the complementary for the mass balance method.