Objective To investigate the expression of vascular endothial growth faetor(VEGF) proliferating cell nudear antigen(PCNA) and their biological significance was studied in papillary thyroid carcinoma and normal thyroid tissues. Methods Immuohistochemismy S-P technique was used to examine the expression of VEGF and PCNA in specimens of 58 papillary thyroid carcinoma and 19 normal thyroid tissues. Results The positive rates of VEGF and PCNA in papillary thyroid were higher than those in normal thyroid tissues( P＜0.01 ), and a positive correlation between these two expressions existed in papillary thyroid carcinoma. Conclusion VEGF and PCNA could be regarded as parameters to evaluate the biological behavior and they might be the indices for juvant diagnosis and evaluation of papillary thyroid cancer.

Objective:To discuss the clinical feature and treatment of second mesiobuccal canal (MB2)of permanent maxillary molars. Methods:The root canals of 106 maxillary first molars and 80 maxillary second molars were explored with small size K files. The frequency and morphology of root canals were recorded. The canals of all cases were instrumented by Ni-Ti rotary instruments Hero 642, and obturated with lateral condensation technique. The efficiency of preparation and obturation was analyzed with radiographs before and after treatment. Results:The frequency of MB2 canal in maxillary first and second molars were 82.1% and 45.0%. Most MB2 was very fine and negotiated by size 8 or 10 file combined with EDTA. No transportation, ledge, or perforation was found except that one hand file was separated. Good result of treatment was achieved in most cases. Conclusion:The incidence of MB2 canals of maxillary first molars was higher than that of second molars, and most of them were very fine. The MB2 canals can be negotiated with small size file combined with EDTA, and shaped well with Ni-Ti rotary instruments.

Objective To isolate and identify the pathogenic bacterial strain Myroides odoratus from municipal sewage in Zhenjiang city.Methods With selective medium,a pathogenic bacterial strain JD23 was isolated from the city sewage.Combining the modern molecular biology technology methods and bioinformatics methods,its taxonomic status was confirmed by 16S rDNA gene sequence homologous analysis and phylogenetic tree.The traditional experiments of physiology,biochemistry and morphology were also studied.Results Strain JD23 exhibited the highest levels(98%) of similarity to the Myroides odoratus M58777.2.Combining all the results of several methods and morphological observation,strain JD23 was finally identified as the bacterial classification Myroides odoratus.Conclusion There was a pathogenic microorganism,Myroides odoratus,in municipal sewage of Zhenjiang city.

Objective:To evaluate the shaping ability of nickel-titanium (NiTi) rotary instrument Hero 642 combined with SX of ProTaper in moderately and severely curved root canals.Methods:42 teeth with moderately and severely curved canals in 42 patients were prepared by Hero 642 and SX of ProTaper using crown-down technique and obturated with lateral condensation method. The efficiency of the preparation and obturation were analyzed by radiographs before and after treatment. Results:After preparation by the rotary instrument the original curvature and alignment of the canals were kept. No transportation, apical blockage, ledge or perforation was found in all cases. In one case,the file was broken during operation.After treatment light pain was complained in 4 cases and the pain alleviated and disappeared spontaneously. Conclusion:The NiTi rotary instrumentation technique with Hero 642 and ProTaper can be used to prepare curved root canals effectively and quickly.

Objective To explore the changes in fractional anisotropy (FA) among cerebral infarction pa-tients using MR diffusion tensor imaging (DTI), and to verify the relationship between any FA changes and functional recovery. Methods Thirty-eight cerebral infarction patients were divided into two groups according to their recovery level using Brunnstrom's criteria. All the patients accepted routine MR and DTI examination, and FA values were measured during the acute, subacute and chronic stages of their recovery. Results Average FA values in the lesion area were significantly lower than in the corresponding contralateral area, and were highest daring the acute stage.There was no significant difference between the subacute and chronic stages. Conclusions The FA values of cere-bral infarction patients change during the different stages of recovery with a certain regularity. This may be valuable for clinical treatment and prognosis.

Applying modern computer network information technology and digital ECG technology,ECG experiment education software system with learning theory,reading ECG picture and practicing report sheet was developed by the Microsoft Visual C++ 6.0 language as the main programming language,and by present widely used relational database MySQL as the backend database.This education software system,used by more than 2 000 different professional students in 2013 and 2014,can be effective to improve their interpretation skills in ECG,and expands the non-class space of ECG education by the modern network technology.It is worthy of popularization as a powerful helper of ECG teaching.

Objective To demonstrate the imaging characteristics of pancreatic neuroendocrine neoplasm (P-NEN).Methods The CT and MRI findings of 43 cases of P-NEN confirmed by pathology were analyzed,and compared with pathological results.Results Mean size of 43 lesions in 43 P-NEN patients were (25.00± 1.82)mm.Necrosis cystic degeneration were found in 26 lesions.In CT plain scan,15 lesions were isodense,28 lesions were hypodense.All of 43 lesions were hypointensity signal on T1WI;27 lesions were hypointensity signal,16 cases were isointensity signal on T2WI.Twenty-seven lesions showed envelope;30 lesions showed clear outline.In enhancement images,24 lesions were marked enhancement since arterial phase;8 lesions showed delayed enhancement;5 lesions showed centrality enhancement;6 lesions showed wild enhancement.Conclusion P-NEN has some characteristics on CT and MRI scanning,imaging can play an important role in diagnosis for P-NEN.

Objective·To study the hemodynamic parameters of uterine at midluteal phase in patients with early recurrent spontaneous abortion (ERSA) and the effect of aspirin on them. Methods·Transvaginal color Doppler ultrasonography was used to measure the parameters of uterine blood flow and the endometrial thickness at midluteal phase of 271 women with ERSA (ERSA group) and 66 women without a history of recurrent spontaneous abortion (control group). Then ERSA group were administered with aspirin 50 mg/d orally for 2 months and other individualized treatment, and the effect of aspirin on parameters of uterine blood flow and the early pregnancy outcome of them were observed. Results·At midluteal phase, the endometrium was significantly thinner in ERSA group than that in control group. Pulsatility index (PI) of endometrial blood flow and mean PI (mPI), mean resistance index (mRI), and mean systolic/diastolic ratio value (mS/D) of uterine arteries were statistically significantly higher in ERSA group in comparison to control group (P<0.05). Following aspirin treatment, resistance to uterine blood flow reduced significantly in ERSA group (P=0.000), and the endometrial thickness increased in the patients with endometrial thickness less than 7 mm (P=0.000). Only 163 ERSA patients were re-examined by transvaginal color Doppler ultrasonography after aspirin treatment, among whom 136 women was pregnant after individualized treatment. Among these pregnant patients, 97 cases were pregnant for more than or equal to 12 weeks, while 10 cases aborted during the first 12 weeks, and the early pregnancy outcomes of the other 29 cases were still unclear. Conclusion·In comparison with normal fertile women, ERSA patients have significantly higher resistance to uterine blood flow and thinner endometrium. Aspirin can improve uterine blood perfusion, which improves early pregnancy outcome.

Objective · To investigate the status of depression and anxiety in patients with recurrent spontaneous abortion (RSA) and the possible influence factors and to provide theoretical support for further psychological intervention. Methods · RSA patients and women with no history of RSA were invited to complete a questionnaire, including basic information, Self-Rating Depression Scale (SDS) and Self-Rating Anxiety Scale (SAS). All data were analyzed by SPSS. Results · Of all 1064 cases included in this study, 725 were RSA cases, 217 cases with one spontaneous abortion and 122 in control group with no history of spontaneous abortion. Our results showed that both RSA patients and patients with one spontaneous abortion have significantly higher SDS and SAS scores than control group. Furthermore, non-pregnant RSA patients with lower education level, lower household income and 3-5 years of marriage have significantly higher levels of depression and anxiety. Patients with multiple miscarriages (≥4), history of induced abortion and no live birth, score significantly higher in SDS. Conclusion · Whether pregnant or not, RSA patients are much easier to become depressive and anxious, which may be associated with education level, household income, length of marriage, numbers of pregnancy losses and previous live birth. Women with one spontaneous abortion also show a significant higher tendency of depression and anxiety. These patients should be given proper psychological intervention if necessary.

AIM: To detect whether N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) inhibits epithelial-mes-enchymal transition in A549 cells induced by TGF-β1 through suppressing the expression of heat shock protein 27 (HSP27) and zinc finger proteins Snail (including SNAI1and SNAI2) which ultimately inhibited the deposition of type I and type III collagens.METHODS:The colocalizations of HSP27 and SNAI1/SNAI2 respectively on A549 alveolar epi-thelial cells induced by TGF-β1 were measured by confocal microscopy .The expression of HSP27, SNAI1 and SNAI2 at mRNA level was detected by real-time PCR.Western blotting analysis was used to detect the expression of HSP 27, SNAI1 and SNAI2 on epithelial-mesenchymal transition in A549 cells induced by TGF-β1 and also the deposition of type I and type III collagens in A549 cells transfected with HSP27shRNA prior to TGF-β1 stimulation.RESULTS: Compared with control group, TGF-β1 increased the expression of HSP27, SNAI1, SNAI2, type I and type III collagen, which decreased significantly followed by Ac-SDKP intervention.The expression of SNAI1, type I and type III collagen decreased signifi-cantly after transfected with HSP27shRNA in A549 cells, which had the similar effect on Ac-SDKP intervention.CON-CLUSION:Ac-SDKP inhibits the transition of cultured A 549 cells to myofibroblasts and attenuates collagen synthesis by suppressing the expression of HSP 27 and zinc finger proteins SNAI 1 and SNAI2.