Objective To investigate the effect of hydroxyl safflor yellow A on mediating blood lipid and to explore its primary mechanism.Methods Fifty KM mice were divided into five groups randomly:control group(A),hyperlipidemia model group(B),high-dose group(C),mid-dose group (D) and low-dose group(E).C,D and E group were injected by hydroxy safflor yellow A with 10,40 and 70 mg.kg-1 day-1respectively,while A and B group were both injected by saline with 0.4 ml day-1,the administrations were kept on three days.The levels of serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C) and malondialdehyde (MDA) were assayed,simultaneously the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were assayed after 18 hours.Results Compared with the control group,the serum TC (4.09+0.2) mmol/L,TG (0.96±0.15) mmol/L,LDL-C (5.87±0.17) mmol/L,HDL-C (0.83±0.21) mmol/L,MDA (8.26+1.05) nmol/ml,and the activities of SOD (330.18 ± 11.45 ) U/ml,GSH-Px (1023.54±25.34) U/ml of model group injected high doses of hydroxysafflor yellow A were statistically significant.Conclusion Hydroxy safflor yellow A had the function of mediating blood lipid.Anti-oxidation could be the mechanism.

The activities of low density lipoprotein (LDL) receptors in 15 organs and tissues from 5 human fetuses of the ages of 22 to 34 weeks were determined using a membrane filter assay of the specific binding of ~(125)I-LDL to tissue homogenates. The results showed that the adrenal cortex (465 ng/mg protein) had the highest activity of ~(125)I LDL specific binding seven times than that of the medulla. Adipose tissue (214 ng/mg protein) and liver (102 ng/mg protein) ranked second. Relatively high bindings were also observed in the skeletal muscle (89 ng/mg protein), brain (65 ng/mg protein), kidney (60 ng/mg protein) and spinal cord (56ng/mg protein). It was found that the activity of LDL receptors in the central nervous system of human fetus was higher than that of cows, of human adultsand of humam fetuses of 16 to 20 weeks as reported by other investigators. Relatively high level of LDL receptor mRNA in human fetal brain was also found by ~(32)P-cDNA probe hybridization analysis. It is suggested that the growing and developing central nervous system of human fetus reqiresmore cholesterol. In addition, We also observed preliminarily that the tendency of LDL receptors activities in liver and adrenal gland cortex were gradually inereased with fetal age.

Objective: To investigate the epidemiological characteristics of pulmonary tuberculosis in Taizhou City from 2016 to 2021, so as to provide the evidence for formulating the pulmonary tuberculosis control measures. Methods: The epidemiological data of pulmonary tuberculosis cases in Taizhou City from 2016 to 2021 were retrieved from the Tuberculosis Management Information System and Infectious Disease Surveillance System of Chinese Disease Prevention and Control Information System, and the trends in the incidence of pulmonary tuberculosis and regional, population and temporal distributions of pulmonary tuberculosis were analyzed using a descriptive epidemiological method in Taizhou City from 2016 to 2021. Results: A total of 15 621 pulmonary tuberculosis cases were reported in Taizhou City from 2016 to 2021, with annual mean reported incidence of 42.12/105. The incidence of reported pulmonary tuberculosis reduced from 43.74/105 in 2016 to 36.31/105 in 2021, with an annual decline rate of 3.65% (χ2trend=34.172, P<0.001). There were 7 606 cases positive for pathogenic tests (48.69%), and the positive rate of pathogenic tests appeared a tendency towards a rise from 2016 to 2021 (χ2trend =470.906, P<0.001). The reported incidence rates of pulmonary tuberculosis were 57.83/105 and 25.46/105 in men and women, and a high incidence rate was reported in patients at ages of 20 to <25 years (72.80/105) and 65 years and older (84.75/105). Farmer was the predominant type of occupation (8 365 cases, 53.55%), and the cases peaked from March to August, with the greatest seasonal index in May (119.76%). A high annual incidence rate of pulmonary tuberculosis was reported in Xianju County (52.51/105) and Wenling City (49.98/105) from 2016 to 2021, and the a high constituent ratio of patients positive for pathogenic tests was seen in Sanmen County (58.71%) and Luqiao District (53.24%). Conclusions The reported incidence of pulmonary tuberculosis showed a tendency towards a decline, and the proportion of pulmonary tuberculosis patients positive for pathogenic tests showed a remarkable increase in Taizhou City from 2016 to 2021. The number of patients peaked in spring and summer. In addition, high incidence of pulmonary tuberculosis was seen in Xianju County and Wenling City.

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Objective To explore the effects and mechanism of electroacupuncture on acute hypertension in rats. Methods 36 male Wistar rats were randomly divided into control group (n=8), model group (n=8), drug group (n=10) and electroacupuncture group (n=10). The acute hypertension rat model was induced with injecting adrenalin into abdomen. Blood pressure was measured and blood adrenalin and noradrenalin levels were tested after electroacupuncture in points Baihui DU-20 and Zusanli ST36. Results The systolic and diastolic pressures were lower in the drug group and the electroacupuncture group than in the model group (P<0.05), and there was no difference between the 2 groups (P>0.05). The adrenalin and noradrenalin levels were higher in the model group than in the control group (P<0.05), but they were lower in the drug group and the electroacupuncture group than in the model group (P<0.05). Conclusion The electroacupuncture could reduce the blood pressure of acute hypertension rats by decreasing blood adrenalin and noradrenalin levels.

OBJECTIVE To establish secretory otitis media(SOM) model induced by endotoxin and evaluate its reliability. METHODS Healthy 50 Sprague-Dawley rats of either sex( 250-300g)were randomly divided into experimental group(ET group) and control group(NS group),with 30 and 20 rats respectively. 35ul volume of 1mg/ml endotoxin from Pseudomonas aeruginosa and same amount of saline(NS) were transbullaly injected into the right tympanic bullas of the two groups. Six rats of ET group and four rats of NS group were killed postoperative on 6h,1d,3d,7d,14d respectively. The temporal bones were harvested for histological study. The left tympanic bullas served as control. Another four rats(each group 2 rats) were used for SEM study with the same method. One rat was killed and harvested for SEM observation of mucocilliary transporting system on the tympanic ostium of Eustachian tube. RESULTS ①LM(light microscope):The epithelial layer and subepithelial space(SES) were thicked mildly in ET group at 6 hours,and more heavily at 1 day with inflammatory cells infiltration (mostly polymorphonuclear leukocytes,PMNs). At 3 days,the same changes were observed most severely. By 14 days,the mucosal lining returned to normal. No obvious changes were observed in the NS group. ②SEM(scan electrical microscope):In ET group,the impairment of mucociliary transport system such as lodging,shedding of cilias were observed at 3 days and returned to normal at 14 days. No abnormal changes were observed in NS group. CONCLUSION Endotoxin can induce SOM with inflammatory changes peak at day 3 and return to normal by day 14.

BACKGROUND:Traumatic auricle defectsin upper 1/2 or lower 1/2,seriously involve theauricular cartilage and skin blood vessels. The autogenic rib cartilage graft and acelular dermal matrix have good histocompatibility, and expanded flapis a kind of thin and achromatic tissue for skin defect repair. OBJECTIVE:To explore theapplication ofacelular dermal matrix and expanded flap in half auricular reconstruction,and to find out the fine carving and anastomosis of autogenic rib cartilage graftas wel as its similarities with the ear and clinical significance. METHODS:Eight cases of half auricular defects were treated with expanded flap, autogenic rib cartilage graft, fine anastomosisofautogenic rib cartilage graft and residual earfor half auricular reconstruction,during which theacelular dermal matrixwas usedto promote residual ear docking and skul auricle angle formation. The reconstructionwasperformed in three stages:first,anexpander(volume, 80mL)wassubcutaneously implanted attheretro-auricular area;second, the auricular defects were reconstructed with fine rib cartilage graft, acelular dermal matrix and auriculoplasty;finaly, acelular dermal matrixwas usedto promote residual ear docking. Thenthehalf auricular reconstructionwas evaluatedby objective measurement and subjective rating. RESULTS AND CONCLUSION:Half auricular reconstruction was successful in al the eight caseswithout obvious complications, and the cartilage grafts were in good condition.During thefolow-up,thereconstructed auriclewasshapedwel andformed a good involution withtheresidualauricle. In addition, the flange was smooth withoutobviouscolor difference and edema, and its position, size and shape were consistent with those of thecontralateralone. Afterthefolow-up of 6 months, objective indicators showed that the affected side had no significant differencefromthe contralateralone(P> 0.05). In conclusion,theacelular dermal matrixcanobviously decrease the complications of the cartilage grafts andcontributeto agood shaped auricle;and half auricular reconstructionby expanded flap, autogenic rib cartilage graft,andfine anastomosis of autogenic rib cartilage graftcanachieve significant clinical effects.

BACKGROUND:At present, the common filing materials used to correct secondary unilateral cleft lip nasal deformity include conchae cartilage, costal cartilage, Medpor implants, expanded polytetrafluoroethylene (ePTFE), alogenic acelular dermal matrix. OBJECTIVE:To analyze the therapeutic effects of comprehensive procedures with alogenic acelular dermal matrix or ePTFE for secondary unilateral cleft lip nasal deformity. METHODS: Thirty-six patients with secondary unilateral cleft lip nasal deformity were enroled, including 19 males and 17 females, aged 15-32 years. Alogenic acelular dermal matrix (n=22) or ePTFE (n=14) was used to correct nasal base colapse deformities. Anthropometry method was employed to make measurements. Fixed-point measurement was performed based on patient's pictures before and after correction. Long-term effects of these two kinds of filing materials were analyzed and assessed objectively and quantitatively. RESULTS AND CONCLUSION: After the folow-up of 6 months, al the patients were satisfied with their results, and no infection and no exposure occurred. The treatment effect of the alogenic acelular dermal matrix group was excelent in 16 cases and good in 6 cases; there were 10 cases of excelent and 4 cases of good in the ePTFE group. The objective indicators in the two groups were al improved at 6 months after correction (P < 0.05), but there was no difference between the two groups (P > 0.05). These findings indicate that alogenic acelular dermal matrix or ePTFE is useful to correct secondary unilateral cleft lip nasal deformity.

Objective To study the effect of 17-β estradiol on Collagen Ⅰ,lysyl oxidase like 1 (LOXL1),fibrinectin( FN )after stretch in the pelvic function dysfunction(PFD) patients vaginal wall fibroblasts cultured in vitro,and the effect in PFD pathogenesis.Methods Twelve patients with pelvic function dysfunction who under surgery in the second Hospital,Hebei Medical University from October 2009 to September 2010 were collected vaginal wall organization in this study.Use of tissue and collagenase digestion method for primary culture in the vaginal wall fibroblasts.After stretch with different concentrations of 17-β estradiol cultured fibroblasts.Total mRNA was extracted from fibroblasts and gene expression of Col Ⅰ,LOXL1,FN were measured.Results After stretch,the expression of Col Ⅰ ( 1.1872 ± 0.0733 vs 1.5035 ±0.0733,t =-4.815,P ＜0.01),LOXLI(0.7724 ±0.1873 vs 1.0855 ±0.0805,t =-5.111,P ＜0.01 ) were significant higher than before( P ＜0.01 ),the expression of FN (0.4290 ±0.1168 vs 0.4215±0.0830) was not significantly different ( P ＞0.05).The expression of all factor Col [ (3.0809 ±0.1862),LOXLI ( 1.5863 ±0.3241 ),FN( 1.1418 ± 1.0030) in high drug concentrations of 17-β estradiol were increased significant compared with controlled groups( P ＜ 0.01 ).Conclusions 17-β estradiol increased synthesis of extracellular matrix components from cultured vaginal wall fibroblasts of patient with pelvic floor dysfunction after stretch.Stretch and estrogen may play an important role in PFD pathogenesis.

Ischemic stroke is a catastrophic event in young adults. It may cause death,disability, incapacity and decline in the quality of life. When the neurologists are facing the suspected ischemic stroke in young adults, the greatest challenge of diagnosis is to identify the causes. This article reviews the risk factors, etiological diagnosis as well as its associated disorders and dysfunction in young adults.