Objective To analyze the hypocaloric nutrition(PN) in perioperative of patients with congenital hypertrophic pyloric stenosis ,and to provide reference basis for its clinical application .Methods 40 patients with congenital hypertrophic pyloric steno-sis were choseen in our hospital .All patients were divided into observation group and control group ,20 cases in each group .After the conventional support treatment ,patients in observation group were gave hypocaloric nutrition of 70 -90 kcal/kg ,patients in control group were gave hypercaloric nutrition of 100-200 kcal/kg .After 5 days ,nitrogen balance ,serum protein and body compo-sition were observed in the two groups .Results The nitrogen discharge was increased after parenteral nutrition ,after 2 days reached to peak ,and then began to decline slightly .Under the condition of the same nitrogen intake ,the nitrogen balance in observa-tion group was 10 .65 g/d ,which was significantly lower than 16 .40 g/d in control group (P<0 .05) .The difference of total pro-tein ,albumin ,prealbumin ,transferring were not obvious before parenteral nutrition (P>0 .05) .After parenteral nutrition ,the total protein ,albumin ,prealbumin ,transferrin in observation group were (58 .22 ± 4 .63)g/L ,(43 .42 ± 3 .53)g/L ,(269 .65 ± 31 .24)g/L , (2 .83 ± 1 .23)g/L ,respectively ,which were higher than that in control group (42 .11 ± 3 .52)g/L ,(35 .57 ± 3 .82)g/L ,(252 .34 ± 27 .03)g/L ,(2 .20 ± 1 .30)g/L ,the differences were statistically significant (P<0 .05) .But the difference of CRP level was not ob-vious (P>0 .05) .Observation group had greater TBF and F% but decreased LBM versus control group (588 .7 ± 66 .8)g vs . (492 .8 ± 65 .6)g ,(17 .5 ± 1 .9)% vs .(15 .0 ± 1 .8)% ,(2 498 .5 ± 118 .9)g vs .(2 604 .7 ± 125 .4)g .The differences were statistical-ly significant(P<0 .05) .Conclusion Hypocaloric nutrition can meet the metabolic needs of patients with congenital hypertrophic pyloric stenosis .Hypocaloric nutrition play a positive role in improving nutritional status and can improve the postoperative rehabilitation .

Objective To explore the correlation of depression and ordinary status of stroke patients. Methods 260 stroke patients of our hospital from June 2012 to December 2013 were selected and divided into two groups,the observation group had depression after stroke,the control group didnˊt have depression after stroke,the ordinary status and society status of 2 groups were compared.Results Women accounted for 79.51% of the patients in the observation group,Below the level of high school education Patients accounted for 72.28%,patients with cardiovascular disease accounted for 65.06%;the patients in the observation group who was higher than 6000 yuan, accounted for 62.86%,which was lower than 86.67% of the control group (χ2 =10.21,P <0.05).The subjective support and the score of social support utilization of patients in the observation group were significantly lower than those of the control group(t =2.98,3.19,P <0.05).Conclusion Depression is a common complication for stroke patients,causing by many reasons,including gender,education level,CVD,family income and so on.

Objective To investigate the effect of muscle fatigue on linear and nonlinear dynamics of sEMG from biceps brachii during maximal voluntary isometric contractions.Method Ten young volunteers subjects performed non-fatiguing isometric contractions with four load levels and fatiguing maximal voluntary isometric contractions.Linear and nonlinear analysis were used to examine the sEMG signals and the changes of sEMG signals when MVC decreased to levels of 80% MVC,70% MVC,60%MVC and 50%MVC compared with the signals of sEMG during non-fatiguing contractions.Result Statistical analyses revealed that AEMG and %DET were significantly higher at fatigue states than those at non fatigue states,while MPF and C(n)were significantly lower than those at non fatigue states.With the development of muscle fatigue,MPF and %DET significantly decreased during maximal effort,while AEMG and %DET stayed stable.The interaction of load level and fatigue state for AEMG,MPF and C(n)were significant.Conclusion MPF and C(n)significantly decrease during maximal voluntary isometric contractions.sEMG dynamics between fatigue state and non-fatigue state is significantly different.

Objective To investigate the expressions of microRNA-21,microRNA-143,and microRNA-145 in the sera of patients with nasopharyngeal carcinoma (NPC),and their assessment vales in the recurrence,metastasis,and prognosis of NPC patients.Methods From January 2012 to January 2014,80 NPC patients in our hospital Department of Internal Medicine and Head and Neck Surgery was used as tumor group,80 cases of healthy volunteers as control.The real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the expression levels of microRNA-21,microRNA-143,and microRNA-145 in the sera.Results Expression of microRNA-21 in NPC patients was significantly higher than that in healthy control group with statistically significant difference (P ＜ 0.05).The expressions of microRNA-143 and microRNA-145 in NPC patients was significantly lower than those in healthy control group with significant difference (P ＜ 0.05).At the same time,those microRNAs were significantly associated with tissue differentiation,invasion,and metastasis.Conclusions Increased microRNA-21 expression level in NPC patients,and decreased expression of microRNA-143 and microRNA-145 in NPC patients play an important role in differentiation,invasion,and metastasis in the development process of NPC.microRNAs can be used as a new index in the auxiliary diagnosis of NPC and the evaluation of recurrence,metastasis,and prognosis evaluation.

Cytokine storm plays an important role in the pathogenesis of liver failure.It is closely related to hepatocyte necrosis in the clini-cal course and the prognosis of this disease.With Kupffer cells as a clue,it is elucidated that related cells and cytokines influence each other and jointly function in the development and progression of liver failure,leading to serious liver tissue damage and necrosis.Cytokine -based research can help improve early diagnosis,disease assessment,and individualized treatment for liver failure.

AIM:To investigate the effects of angiotensin Ⅱ(AngⅡ)blockers on TNF-? and the relationship between TNF-? and proteinuria in patients with chronic glomerulonephritis(CGN).METHODS:32 patients examined by biopsy as primary CGN with proteinuria were treated by benazepril,valsartan or combination with benazepril and valsartan for 8 to 12 weeks respectively.The TNF-? levels in serum and urine,24 hours proteinuria quantity,and serum creatinine level were assayed and recorded respectively before and after 8 to 12 weeks therapy.RESULTS:The TNF-? levels in serum and urine and 24 hours proteinuria quantity were reduced significantly after therapy [(7.8?3.9)vs(5.0?1.6)fmol/mL,(11.9?6.8)vs(8.3?3.5)fmol/mL,(2.6?2.0)vs(1.0?0.5)g,respectively;P

AIM: To observe the therapeutic effect of valsartan on cardiac hypertrophy and expression of endothelin-1(ET-1) in myocardium of rats with chronic renal failure and explore the mechanism.METHODS: 24 Sprague Dawley male rats were selected,and performed five-sixths nephrectomy to produce chronic renal failure model.Two weeks after the surgery,the rats were randomly divided into model group,valsartan group,and a sham group was established serving as control group.Systolic blood pressure,BUN and serum creatinine were measured at ten weeks after operation,then the rats were sacrificed to take the hearts for pathological histological examination.Hybridization in situ was used to examine the transcription of ET-1 mRNA in myocardium.RESULTS: Systolic pressure,heart weight,heart weight index,left ventricular weight mass(LVM) and left ventricular weight mass index(LVMI) in model group increased significantly tenth week after operation.Systolic pressure,heart weight,heart weight index,LVM and LVMI were decreased markedly in rats with five-sixths nephrectomy by valsartan(P

Objective To compare the concentration of nuclear factor-kappa B (NF-κB), MCP-1 and RANTES in peritoneal fluid in women with endometriosis and normal peritonal fluid ,To explore the relationship between these cytokines with pathogenesis of endometriosis. Methods There were 2 groups:the endometriosis group and the control group.Double-antibody sandwich ABC-ELISA technique was adopted for determinating concentration of NF-кB、MCP-1 and RANTES in peritoneal fluid. Results Peritoneal fluid level of NF-κB MCP-1 and RANTES in endometriosis patients was higher than the control group (P < 0.05). NF-κB and MCP-1, NF-κB and RANTES expressed positive correlation by linear correlation analysis in both EMs group and control group , correlation coefficients were 0.385, 0.569(EMs group), 0.474, 0.388(P < 0.05). Conclusions NF-кB、MCP-1 and RANTES are mutual affection and promote endometriosis in the pathogenesis.

Objective To evaluate clinical application of VCS technology in diagnosis for acute leukemia and its subtypes by LH750 automated hematology analyzer. Methods A total of 178 leukemia patients (105 acute granilocytic leukemia, 42 acute lymphocytic leukemia, 31 acute monecytic leukemia)and 151 non-leukemia patients (86 granulocytic abnormal cases, 35 lymphocytic abnormal cases,30 monocytic abnormal cases) were enrolled into this study. Peripheral blood samples were analyzed by both Coulter LH750 automated hematology analyzer and manual microscopic examination. Microscopic examination was used as reference to evaluate the diagnosis value of VCS technology in blast detecting. VCS parameters were compared for AGL subsets. The clinical value of VCS technology in diagnosis for different acute leukemia was also evaluated by combining VCS scattergram and suspect flags provided by LH750. Results Compared with manual examination, a sensitivity of 95.5% (170/178) and specificity of 95.4% (144/151) were achieved by VCS technology in detecting blasts in acute leukemia and nonleukemia patients. In patients with AGL, the MNV, MNV-SD, MNC and MNC-SD (224.40 ± 23.37,37.40 ±12. 31,145. 80 ±7. 93,24. 79 ±5. 18) were higher than granulocytic abnormal patients( 169.96 ±11.50,29.21 ± 5.27,133.30 ± 5.50, 10.62 ± 4.09) and the differences were statistically significant ( t values were 16. 832, 5. 148, 5. 735, 19. 953 respectively, P < 0. 01 ). On the contrary, MNS ( 122. 90 ±6. 35) in AGL patients was significantly lower than granulocytic abnormal patients( 131.00 ± 5. 69, t =-7.64, P<0.01). In patients with ALL, the MLV, MLV-SD, MLC, MLS, MLS-SD (97.60 ± 13.40,22. 35 ± 7.94,110. 00 ± 4. 60,77. 60 ± 19.00,20. 61 ± 3.30 ) were higher than lymphocytic abnormal patients(82. 10 ± 3.00, 14.41 ±2.35, 100.60 ±2.70, 48. 10 ±3.50, 17.60 ± 1.60). The data also showed a statistical significance (t values were 7.576, 6. 118, 4. 041, 9. 353, 2. 988 respectively, P <0.05). In patients with AMOL, the MMV, MMV-SD, MMC, MMC-SD, MMS(197. 30 ±20.50,30.47 ±6.58,123.20 ± 10. 10,6.57 ± 1.57,98.00 ± 5.60) were significantly higher than monocytic abnormal patients( 167.80 ± 15.77,21.90 ± 9. 64,113.60 ± 6. 73,5.20 ± 3. 21,84. 20 ± 14. 35 ) and the differences were statistically significant (t values were 6. 332, 4. 033, 4. 650, 2. 993, 6. 273 respectively , P <0. 01 ).The differences of MNV, MNV-SD, MNC, MNC-SD, MNS-SD for different AGL subtypes were statistically significant ( F values were 21.2, 169. 5, 13.6, 3. 6 and 98.6 respectively, P <0. 01 ). For AGL, ALL and AMOL, the ratios of normal blast distribution in scattergram were 81% (85/105), 74% (31/42) ,and 74% (23/31). Conclusions The VCS parameters can reflect the changes of peripheral white blood cell morphology sensitively. VCS technology can diagnose acute leukemia and its subtypes efficieutly.

Objective To study the changes of biochemistry indexes in blood serum and bone mineral density (BMD) of secondary osteoporosis after spinal cord injury (SCI) in rats. Methods 120 male Wistar rats were divided randomly into 12 groups (n=10). The rats in control groups were only removed larmina with dura intact, without spinal cord injury at the level of the tenth thoracic vertebrae, the rats in experimental groups were also removed larmina, then paraplegic model was made by the method of Allen's (60gcm). The changes of biochemistry indexes in blood serum and BMD were observed. Results Compared with control groups, the concentrations of alkaline phophatase (ALP) were decreased at the 1st and the 3rd week in experimental groups (P0.05). BMD of tibia was decreased at the 7th and 11th week in experimental group (P