Objective To evaluate the clinical efficacy of artery chemoembolization in the treatment of cervical cancer.Methods Infusion chemotherapy and embolization were implemented in 28 patients with cervical cancer who admitted in our hospital from May 2009 to May 2012.After treatment for 14d to 21d,according to the clinical situation,choose to continue chemotherapy embolization or surgical treatment.Results 28 patients after implementation of transcatheter arterial chemoembolization therapy,the lumbosacral pain,vaginal fluid and bleeding were significantly improved with lesion subsided rate of 89.28％ (25/28).25 patients received radical resection,the surgical results were satisfactory,the amount of bleeding was less.Conclusion Cervical arterial chemoembolization therapy can significantly improve patients' clinical symptoms,and enhance the effect of surgery,reduce the incidence of complications,which has positive significance in improving the quality of life.

In order to reduce hospital acquired infection(HAI)caused by endoscopy examination,explore more effective administration,this article introduces a series of measures and effects about controlling the sources of infection,disinfecting the tool of anesthesia、endoscopy and its appendix、environment in our endoscopy center.

This article introduces the centralized operation and integrated management of the Endoscopy Center of Nanjing General Hospital,expounding the idea of development,mode of management,working model and characteristic structure of the center.

To observe the effect of kidney reinforcing and blood nourishing herbal medicines in treating anovulatory sterility (AS) and its mechanism. Two hundred and seventy six cases of AS with hypopituitarism confirmed by the examination of blood sexual hormones were divided into three groups to receive the treatment of promoting ovulation. Group A was treated with modified Zhenwu Decoction and Reinforcing Qi and Nourishing Yin Powder (one dose per day) from the third day of menstrual cycle (MC) to the ovulating day; Group B with clomiphene (50mg/d) from the fifth day of MC, with human chorionic gonadotrophin (HCG) added, 10?000?U im in the day of follicle maturity and Group C with human menopausal gonadotrophin (HMG) 25 U/d im from the eighth day of MC and then with HCG added, 10 000 U im in the day of follicle maturity. After treatment, the cases were asked to have sexual intercourse for three nights. Ovulation time was monitored by ultrasound B imaging for one time every other day until follicle became maturity (1 8?cm?1 8?cm).Early pregnancy was detected by HCG test.Ninety three MC were ovulatory among 167 MC in Group A, the ovulation rate being 55 68%; 182 among 230 in Group B , the rate being 79 13% and 88 among 134 in Group C , the rate being 65 67%. Multisample ? 2 test showed that the ovulation rate in Group A was lower than that in Group B and Group C (? 2=9 345, P

Objective The purpose of this study was to determine if recombinant ?-defensin-2 can prevent the lung from being injured by pseudomonas aeruginosa infection. Methods Ten male SD rats ( class SPF) weighing 250-300 g were randomly divided into 2 groups ( n = 5 each) : I defensin group and II control group. The animals were anesthetized with intraperitoneal thiopental 10 mg? kg-1 and intubated. 50 ? l of 5 ? 107 PFU? ml-1 adenovirus with or without ?-defensin-2 gene was instilled into the trachea via tracheal tube. 48 h later 200 ? l of 6? 108 CFU?ml-1 pseudomonas aeruginosa ATCC27853 was instilled into the trachea in both groups. All the rats were killed after 24 h and the lungs were removed for (1) histologic examination, (2) determination of ICAM-1 expression in the lung and (3) broncho-alveolar lavage. The number of WBC and pseudomonas aeruginosa in broncho-alveolar lavage fluid (BALF) was counted. Results The number of WBC and pseudomonas aeruginosa in BALF was significantly less in defensin group than in control group. The degree of instologic damage and the expression of ICAM-1 in lung tissue were significantly decreased in the defensin group as compared with control group. Conclusion Recombinant ?-defensin-2 can kill pseudomonas aeruginosa in vivo and modulate the expression of ICAM-1. It can protect the lung against injury induced by pseudomonas aeruginosa infection.

Objective To explore the clinical efficacy and the dynamic changes of serum vascular endothelial growth factor(VEGF),connective tissue growth factor(CTGF),hypoxia inducible factor 1α(HIF-1α)and osteopontin(OPN)levels after transcatheter arterial chemoembolization(TACE)combined with sorafenib in the treatment of advanced hepatocarcinoma(HCC)patients.Methods A total of 113 HCC patients in Cancer Hospital of Taizhou,from September 2013 to December 2014 were elected and were randomly divided into control group(n=56)and experiment group(n=57)according to random number.Control group were treated with sorafenib and experiment group were treated with TACE combined with sorafenib.The serum VEGF,CTGF,HIF-1α and OPN levels were tested and compared using indirect ELISA method preoperative and postoperative 1,3,7 days and which were carried out Spearman correlation analysis.The long-term clinical efficacy and adverse reaction in two groups were statisticed.Results The serum VEGF,CTGF,HIF-1α and OPN levels of two groups postoperative 1 day increased than preoperative(P<0.05).From postoperative one to seven days,the serum VEGF,CTGF,HIF-1α and OPN levels of two groups present downward trend(P<0.05 or P<0.01),and there was significant difference between two groups(P<0.01).The level of HIF-1α significantly positive correlated with the levels of VEGF,CTGF and OPN(r=0.951,0.954,0.929,P<0.05).Compared with control group,the median survival time and 1-year-survival rate of experiment group increased significantly(P<0.01).The incidence of hand-foot reaction,alopecia and diarrhea in experiment group were higher than those in control group(P<0.05),while the others had no significant difference between two groups(P>0.05).Conclusion The levels of VEGF,CTGF,HIF-1α and OPN of HCC patients after treated with TACE combined with sorafenib are lower than that treated with TACE alone,Simultaneously,the survival is prolonged and adverse reactions don't increase.

Objective To observe the effect of leflunomide and cyclophosphamide combined with prednisone in the treatment of idiopathic membranous nephropathy .Methods 30 patients with idiopathic membranous nephropa-thy were randomly divided into two groups ,15 cases in each group .A group was treated by leflunomide combined with prednisone , B group was treated by cyclophosphamide combined with prednisone , 6 months a course .The clinical effect,24h urine protein quantity,serum albumin levels,blood fat,renal functions and adverse reactions were com-pared.Results 3,6 months after treatment ,the remission rates in A group were 60.00%,73.33%,those in B group were 53.33%,66.67%(χ2 =0.965,0.896,all P>0.05).After treatment,24h urine protein quantity,serum albu-min levels in the two groups [A group:(1.33 ±1.25)g/24h,(38.24 ±4.84)g/L;B group:(1.42 ±1.37)g/24h, (37.12 ±5.43)g/L] were significantly lower than those before treatment [A group:(7.34 ±2.75)g/24h,(20.31 ± 7.33)g/L;B group:(7.22 ±2.84)g/24h,(20.46 ±7.73)g/L] (A group:t=6.232,5.734,all P<0.05;B group:t=5.934,5.267,all P<0.05).After treatment,total cholesterol,triglyceride in the two groups [A group:(6.74 ± 1.45)mmol/L,(2.43 ±0.75)mmol/L;B group:(6.63 ±1.45)mmol/L,(2.14 ±0.63)mmol/L] were significantly lower than those before treatment [A group:(11.94 ±2.98) mmol/L,(3.56 ±1.35) mmol/L;B group:(11.53 ± 2.84)mmol/L,(3.24 ±1.46)mmol/L] (A group:t=6.246,5.234,all P<0.05;B group:t=5.256,5.672,all P<0.05).There were no significant differences in urea nitrogen ,serum creatinine between before and after treatment in the two groups(all P>0.05).There were no significant differences in above indicators between A group and B group after treatment (all P>0.05).The incidence rate of adverse reactions in A group (13.33%) was significantly lower than that in B group(40.00%)(χ2 =4.246,P<0.05).Conclusion The clinical efficacy of leflunomide is similar to cyclophosphamide in treating idiopathic membranous nephropathy with fewer adverse reactions .

BACKGROUND: Researches show that erythropoietin (EPO) can stimulate the proliferation and protraction of endothelial progenitor cells to form new vessels, so EPO may play an important role in proliferation and differentiation of bone marrow-derived mesenchymal stem cells (MSCs). OBJECTIVE: To explore the effects of EPO on the proliferation and cell cycle of MSCs. DESIGN, TIME AND SETTING: From July to November 2007, the observation of comparative cell trial was performed at the Hematologic Diseases Institute of General Hospital Lanzhou Military Area Command of Chinese PLA. MATERIALS: Bone marrow liquid specimens were provided by voluntary donors. The informed consents were obtained from all patients, and the experiment was approved by Hospital Ethics Committee. METHODS: Using Percoll solution, MSCs were isolated from bone marrow by the method of density centrifugation. Flow cytometry was applied to detect the cell cycles of the second and third passages. MSCs were incubated with different doses of EPO (0.25, 0.50, 1.00, 2.00, 5.00 U/mL) in serum free culture media, and cells cultured with no EPO were regarded as control. All cells were cultured for 24, 48, and 72 hours. MAIN OUTCOME MEASURES: ①Proliferation of MSCs was measured by MTT assays after 24, 48, and 72 hours; ②Cell cycle was measured by flow cytometry assays after incubated with 10 U/mL EPO for 72 hours. RESULTS: After MSCs was incubated with EPO, the cell proliferation index was significantly increased in a dose and time dependent manner. The effects on the proliferation of MSCs were highest in 5 U/mL group. Compared with the control group, EPO could significantly decrease G0 /G1 ratio, and increase S and G2/M stage ratio (P

Objective To investigate the effect of muscle fatigue on linear and nonlinear dynamics of sEMG from biceps brachii during maximal voluntary isometric contractions.Method Ten young volunteers subjects performed non-fatiguing isometric contractions with four load levels and fatiguing maximal voluntary isometric contractions.Linear and nonlinear analysis were used to examine the sEMG signals and the changes of sEMG signals when MVC decreased to levels of 80% MVC,70% MVC,60%MVC and 50%MVC compared with the signals of sEMG during non-fatiguing contractions.Result Statistical analyses revealed that AEMG and %DET were significantly higher at fatigue states than those at non fatigue states,while MPF and C(n)were significantly lower than those at non fatigue states.With the development of muscle fatigue,MPF and %DET significantly decreased during maximal effort,while AEMG and %DET stayed stable.The interaction of load level and fatigue state for AEMG,MPF and C(n)were significant.Conclusion MPF and C(n)significantly decrease during maximal voluntary isometric contractions.sEMG dynamics between fatigue state and non-fatigue state is significantly different.

Objective Culture,expansion and characterization of pseudomyxoma peritonei cells to support foundation for the study of pseudomyxoma peritonei and drug screening.Methods Tumors from 5 cases of human pseudomyxoma peritonei were cultured by collagenase digestion.The cultured cells were then identified as tumor cells by chromosome karyotyping.The secretion of neutral mucopolysaccharide was detected by PAS staining.To simulate the tumor growth condition in vivo,3D culture was applied and the morphology of the cultured organoid was observed with HE staining,xenografts were used to test the tumorigenicity of PMP primary cells.Results The primary culture of pseudomyxoma peritonei cells was successful in all of the 5 cases.2D cultured cells were adherent,polygonal pebble-like arranged,and passed on up to 18 generations.Chromosome karyotype analysis showed that most of the cells were subdiploid karyotype tumor cells.Positive PAS staining suggests that these cells secrete mucus.The morphology of 3D cultured organoid was similar to that of tumor tissues.The tumor formation rate of xenografts was low,and the tumoris not similar to patient's PMP.Conclusions Pseudomyxoma peritonei tumor cells can be cultured and expanded in vitro.