1.Changes in miRNAs expression profiling in the liver of a rat model after reduced-size liver transplantation
Hongqiang GAO ; Zhiqiang LI ; Haifu WANG ; Guoyou XUE ; Jing LIU ; Gang CHEN ; Li LI
Chinese Journal of Tissue Engineering Research 2016;20(5):712-717
BACKGROUND:Liver regeneration is the key factor influencing the prognosis of living donor liver transplantation. There has not been the research on special miRNA of liver regeneration after living donor liver transplantation. OBJECTIVE:To analyze the variation of miRNAs expression profile after rat reduced-size liver transplantation at certain time point, select and verify target miRNA which can provide targeting intervention strategies in liver regeneration after rat reduced-size liver transplantation and provide theoretical evidence for liver regeneration after living donor liver transplantation.METHODS:The reduced-size liver transplantation models were established. miRNAs microarray was used to detect miRNA expression. In differentialy expressed microRNAs, real-time quantitative PCR was utilized to detect target miRNAs. The credibility of miRNAs microarray results was verified. RESULTS AND CONCLUSION:Compared with rat liver tissue in the sham operation group, 11 miRNAs up-regulated in reduced-size liver transplantation, including let-7b-5p, let-7c-5p, miR-101a-3p, miR-103-3p, miR-130a-3p, miR-142-5p, miR-186-5p, miR-199a-3p, miR-21-5p, 221-3p and miR-34a-5p. Four miRNAs were down-regulated, including miR-26b-5p, miR-150-5p, miR-19a-3p and rno-miR-146-5p. PCR test further verified that miR-221-3p and miR-199a-3p expression changes approximated the chip results at 24, 48 hours and 1 week, indicating that results of miRNA microarray were believable. These results verified that it exists variation of miRNAs expression profile after rat reduced-size liver transplantation, which picked out and verified the target miRNAs.
2.The application of bilateral auricular concha cartilage graft in the construction of nasal tip cartilage complex in comprehensive rhinoplasty
Weifeng PENG ; Xinyu OU ; Haifu ZHAO ; Bairong FANG ; Xiancheng WANG
Chinese Journal of Plastic Surgery 2020;36(8):899-904
Objective:To explore the application of bilateral autogenous auricular concha cartilage graft in the construction of nasal tip cartilage complex in comprehensive rhinoplasty.Methods:From January 2016 to June 2019, a total of 521 rhinoplasty patients in the Second Xiangya Hospital and in the Health Medical Cosmetic Hospital received the operation, including 310 patients with primary rhinoplasty and 211 patients with secondary rhinoplasty. There were 12 males and 509 females, aged 18-53 years. Contracted nose patients with severe tissue destruction were excluded. The operation method: the bilateral auricular concha cartilage was cut and combined, including the nasal columella support graft, the anti derotation graft and the cap graft, and the combination of the nasal tip framework complex was constructed to shape the nasal tip framework. At the same time, the expanded polytetrafluoroethylene or silicone prosthesis was carved into willow leaf shape to elevate the bridge of the nose. Patients were followed up after operation. Physicians, patients and a third party were asked to evaluate the preoperative and postoperative photos of more than 6 months to evaluate the surgical effect. The results suggested that the three parties were regarded as significantly effective, two parties satisfied as effective, one party satisfied as average, all dissatisfied as poor; postoperative complications such as infection and prosthesis exposure were judged as invalid. At the same time, the changes of nose tip height and nose length were measured.Results:521 patients in this group got satisfactory appearance effect immediately after operation. After 6-24 months follow-up, the shape of the nose tip remained good, the color of blood circulation was normal, the appearance of the nose tip was natural and beautiful, three-dimensional and straight. Among the 521 cases, 471 cases were significantly improved, 30 cases were improved, 15 cases were acceptable and other 5 cases were poor. The satisfaction rate was 96% (501/521). The average height of nasal tip was(3.5±0.3) mm higher than that before operation. The average length of nose was(4.9±0.4) mm longer than that before operation.Conclusions:The combination of auricular concha cartilage framework and silica gel prosthesis or expanded polytetrafluoroethylene can be used as a routine method for primary rhinoplasty and secondary rhinoplasty.
3.The application of bilateral auricular concha cartilage graft in the construction of nasal tip cartilage complex in comprehensive rhinoplasty
Weifeng PENG ; Xinyu OU ; Haifu ZHAO ; Bairong FANG ; Xiancheng WANG
Chinese Journal of Plastic Surgery 2020;36(8):899-904
Objective:To explore the application of bilateral autogenous auricular concha cartilage graft in the construction of nasal tip cartilage complex in comprehensive rhinoplasty.Methods:From January 2016 to June 2019, a total of 521 rhinoplasty patients in the Second Xiangya Hospital and in the Health Medical Cosmetic Hospital received the operation, including 310 patients with primary rhinoplasty and 211 patients with secondary rhinoplasty. There were 12 males and 509 females, aged 18-53 years. Contracted nose patients with severe tissue destruction were excluded. The operation method: the bilateral auricular concha cartilage was cut and combined, including the nasal columella support graft, the anti derotation graft and the cap graft, and the combination of the nasal tip framework complex was constructed to shape the nasal tip framework. At the same time, the expanded polytetrafluoroethylene or silicone prosthesis was carved into willow leaf shape to elevate the bridge of the nose. Patients were followed up after operation. Physicians, patients and a third party were asked to evaluate the preoperative and postoperative photos of more than 6 months to evaluate the surgical effect. The results suggested that the three parties were regarded as significantly effective, two parties satisfied as effective, one party satisfied as average, all dissatisfied as poor; postoperative complications such as infection and prosthesis exposure were judged as invalid. At the same time, the changes of nose tip height and nose length were measured.Results:521 patients in this group got satisfactory appearance effect immediately after operation. After 6-24 months follow-up, the shape of the nose tip remained good, the color of blood circulation was normal, the appearance of the nose tip was natural and beautiful, three-dimensional and straight. Among the 521 cases, 471 cases were significantly improved, 30 cases were improved, 15 cases were acceptable and other 5 cases were poor. The satisfaction rate was 96% (501/521). The average height of nasal tip was(3.5±0.3) mm higher than that before operation. The average length of nose was(4.9±0.4) mm longer than that before operation.Conclusions:The combination of auricular concha cartilage framework and silica gel prosthesis or expanded polytetrafluoroethylene can be used as a routine method for primary rhinoplasty and secondary rhinoplasty.
4.Expression changes of NaV channel subunits correlate with developmental maturation of electrophysiological characteristics of rat cerebellar Purkinje neurons.
Mingyu FU ; Xiaohong JI ; Lei ZHONG ; Qiong WU ; Haifu LI ; Ningqian WANG
Journal of Southern Medical University 2023;43(7):1102-1109
OBJECTIVE:
To investigate the variations in the expression of voltage-gated sodium (Nav) channel subunits during development of rat cerebellar Purkinje neurons and their correlation with maturation of electrophysiological characteristics of the neurons.
METHODS:
We observed the changes in the expression levels of NaV1.1, 1.2, 1.3 and 1.6 during the development of Purkinje neurons using immunohistochemistry in neonatal (5-7 days after birth), juvenile (12-14 days), adolescent (21-24 days), and adult (42-60 days) SD rats. Using whole-cell patch-clamp technique, we recorded the spontaneous electrical activity of the neurons in ex vivo brain slices of rats of different ages to analyze the changes of electrophysiological characteristics of these neurons during development.
RESULTS:
The expression of NaV subunits in rat cerebellar Purkinje neurons showed significant variations during development. NaV1.1 subunit was highly expressed throughout the developmental stages and increased progressively with age (P < 0.05). NaV1.2 expression was not detected in the neurons in any of the developmental stages (P > 0.05). The expression level of NaV1.3 decreased with development and became undetectable after adolescence (P < 0.05). NaV1.6 expression was not detected during infancy, but increased with further development (P < 0.05). NaV1.1 and NaV1.3 were mainly expressed in the early stages of development. With the maturation of the rats, NaV1.3 expression disappeared and NaV1.6 expression increased in the neurons. NaV1.1 and NaV1.6 were mainly expressed after adolescence. The total NaV protein level increased gradually with development (P < 0.05) and tended to stabilize after adolescence. The spontaneous frequency and excitability of the Purkinje neurons increased gradually with development and reached the mature levels in adolescence. The developmental expression of NaV subunits was positively correlated with discharge frequency (r=0.9942, P < 0.05) and negatively correlated with the excitatory threshold of the neurons (r=0.9891, P < 0.05).
CONCLUSION
The changes in the expression levels of NaV subunits are correlated with the maturation of high frequency electrophysiological properties of the neurons, suggesting thatmature NaV subunit expressions is the basis of maturation of electrophysiological characteristics of the neurons.
Rats
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Animals
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Purkinje Cells/physiology*
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Rats, Sprague-Dawley
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Neurons
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Brain
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Sodium/metabolism*