Full ceramic restorations become more and more popular for their excellent properties. Bond strength between ceramic and composite resin cement is the key factor to success in clinical use. This paper illuminated the importance of ceramic surface treatments and reviewed recent progresses on ceramic surface treatments. The difference of bond strength caused by different dental materials was discussed too.

Objective To assess the diagnostic and preoperative classification value of MSCT after administration of water orally and meglumine diatrizoate enema in diagnosis of colonic neoplasms.Methods The day before examination,40 patients with colon carcinoma had a liquid diet and rountine clean enema,then underwent MSCT scan after administration of water orally and 2% meglumine diatrizoate enema.CT findings were analysed in comparison with postoperative pathologic features.Results There were 2 cases of rectal neoplasms,7 cases of sigmoid colon,6 cases of descending colon,5 cases of transeverse colon,and 20 cases of ascending colon cancer.According to Dukes' staging way,the lesions were B stage in 22 cases,C stage in 12 cases and D stage in 6 cases,the rate corresponding to operation was 89.6%.Conclusion MSCT scan after administration of water orally and 2% meglumine diatrizoate enema in diagnosis of colonic neoplasms has great value.

AIM: To investigate the effect of sodium ferulate on phosphorylation of heat shock protein 27(HSP27) in vascular smooth muscle cells(VSMCs) induced by angiotensin Ⅱ(AngⅡ) and platelet derived growth factor-BB(PDGF-BB).METHODS: Cultured VSMCs derived from rat thoracic aorta were used.The activity of HSP27 was evaluated by Western blotting with specific phospho-HSP27 antibody.RESULTS: The phosphorylation of HSP27 in response to AngⅡ and PDGF-BB was suppressed by sodium ferulate in a dose-dependent manner,with maximal inhibition rates of 39.0%(P

Objective:To evaluate the effects of surface modification with different concentration silica sol through sol-gel processing on bond strength between dental zirconia ceramic and resin cement. Methods:Silica coating was prepared by colloidal silica sol with concentrations of 20%,30%,40% on dental Zirconia ceramic. 32 discs zirconia ceramic specimens were fabricated and randomly divided into four groups respectively after polishing and sandblasting.The ceramic surfaces of different groups were received different treatments:(1)silane couple agent;(2)20% silica coating and silane couple agent;(3)30%silica coating and silane couple agent;(4) 40% silica coating and silane couple agent. Each ceramic block was bonded to light curing composite resin. All samples were stored in distilled water at 37 degrees for 24 h before a shear bond test to measure the bond strength of the resin cement to the ceramic surface. Scanning electrical microscopy (SEM) and energy depressive spectrum (EDS) were used to analyze the silica coating. Results:Contents of surface silicon increased after modification with silica coating prepared by sol-gel method. Bond strengths of groups with silica coating were significantly higher(P

The cis-epoxysuccinate hydrolase (CESH) from Rhizobium strain BK-20 is the key enzyme for L(+)-tartaric acid production. To establish a highly efficient and stable production process, we first optimized the enzyme production from Rhizobium strain BK-20, and then developed an immobilized cell-culture process for sustained production of L(+)-tartaric acid. The enzyme activity of free cells reached (3 498.0 +/- 142.6) U/g, and increased by 643% after optimization. The enzyme activity of immobilized cells reached (2 817.2 +/- 226.7) U/g, under the optimal condition with sodium alginate as carrier, cell concentration at 10% (W/V) and gel concentration at 1.5% (W/V). The immobilized cells preserved high enzyme activity and normal structure after 10 repeated batches. The conversion rate of the substrate was more than 98%, indicating its excellent production stability.
Alginates ; chemistry ; Cells, Immobilized ; Glucuronic Acid ; chemistry ; Hexuronic Acids ; chemistry ; Hydrolases ; metabolism ; Rhizobium ; enzymology ; metabolism ; Tartrates ; metabolism

Objective:To evaluate the toxicity of NaHCO3 coagulated silica gel for coating on the zirconia bonding surface in vivo. Methods:The silica gel was subjected to short term systemic toxicity test by delayed-type hypersensitivity test by oral route and oral mucosa irritation test according to the YY/T 0244-1 996 standards,T1 6886.1 0-2005 /ISO 1 0993-1 0:2002 standards and YY/T 01 27. 1 3-2009 standards respectively.Results:In the short term systemic acute toxicity tested mice there was no significant difference in the weekly food utilization rate and relative body weight growth rate between the experimental and control groups.No abnormality and path-ological change were observed.The delayed-type hypersensitivity test showed that no erythema and edema reaction presented in all the test cavies after 24 and 48 h of stimulation contact,and the sensitization rate was 0.There was no local or systemic pathological change in all the oral mucosa irritation test animals.Conclusion:NaHCO3 procoagulant silica gel has no in vivo toxicity.

Objective:To investigate whether there is another resistance mechanism besides mecA gene in oxacillin-resistant(OR) isolates of Staphylococcus aureus(S.aureus). Methods:There were 130 oxacillin-resistant phenotype isolates of S. aureus. Of these isolates 125 were resistant to more than 3 of 5 non-?-lactams (gentamicin, ciprofloxacin, clindamycin, tetracycline, erythromycin) (OR-R) and 5 susceptible to more than 3 of the 5 non-?-lactams (OR-S) and 14 were oxacillin-susceptible (OS) phenotype isolates of S. aureus and resistant to more than 3 of 5 non-?-lactams (OS-R). All the strains were detected by the two disks diffusion tests with oxacillin (OXA) and oxacillin/clavulanic acid (OXA/CA), by the three-dimensional extract tests of penicillin (PEN) and OXA for penicillinase and oxacillinase, and by PCR tests for mecA. Results:The 130 OR and 14 OS isolates were all oxacillinase-negative with the two disks diffusion tests, all pencillinase-positive and oxacillinase-negative in the three-dimensional extract tests. The mecA gene was detected in 125 OR-R-type and 2 of the 5 OR-S-type isolates, but was not detected in the other 3 OR-S-type nor in any of the 14 OS-R-type isolates by PCR. Conclusion:In a few Staphylococcus aureus strains which are phenotype of oxacillin-resistant and are susceptible to mostly non-?-lactam agents there are both mecA-negative and oxacillinase-negative strains, 2.3% (3/130) of the OXA-resistant strains. The resistance mechanism may be associated with reduced binding capacity of the modified Preexisting PBPs with OXA.

Objective To investigate the protective mechanism of hydrogen sulfide(H2S) on inflammatory and oxidative damage of myocardium due to stress after amputation,and explore the relationship between H2S signal pathway and inflammatory and oxidative damage.Methods Twenty-eight adult male Wistar rats(weighing 260-300g) were randomly divided into 4 groups(7 each):control group,trauma group,NaHS group and PPG group.Rat model of traumatic stress was established in the rats in the 3 latter groups,and the animals were sacrificed 6 hours after modeling.The rats in control group were also sacrificed at the same time point.Rats in NaHS and PPG group respectively received intraperitoneal injection of NaHS(28?mol/kg) or PPG(50mg/kg).The level of H2S in plasma was measured by sensitive sulphur electrode,the activity of CSE in myocardium was determined by methylene blue method,myeloperoxidase(MPO) in plasma and myocardium was measured with ultraviolet spectrophotometry,and malondialdehyde(MDA) in plasma and myocardium was measured by TBA method.Results Compared with the control group,the content of H2S in plasma decreased,MPO and MDA in plasma and myocardium increased 6 hours after amputation(trauma group).Compared with the trauma group,the contents of H2S and MPO in plasma and the activity of CSE in myocardium increased,MDA in plasma and MPO and MDA in myocardium decreased in NaHS group,while the contents of H2S and MPO in plasma and the activity of CSE in myocardium decreased,MDA in plasma and MPO and MDA in myocardium increased in PPG group.Conclusion Inflammatory and oxidation injuries may occur in rat's myocardium after amputation,and H2S can offer a protective effect on myocardium by decreasing inflammatory and oxidative damage.

Objective To establish normal reference ranges for serum cystatin C (Cys C)in relatively healthy middle-aged and elderly(＞50 years)Chinese individuals.Methods A total of 1087 candidates were consecutively selected and serum Cys-C levels were measured by transmission turbidimetry.Frequency analysis and histogram were used to establish the 95％ confidence reference range according to methods provided by CLSI (C28-A2).Results Based on the definition and verification procedures for clinical laboratory reference ranges(CLSI C28-A2,second edition),Cys-C levels of 1087 participants fell within the range of 0.30-1.55 mg/L;Male participants had higher serum Cys-C levels than female participants(Z=-10.19,P＜0.01).The serum Cys-C level increased with age,regardless of gender(R =0.600,P＜ 0.01).Differences in Cys-C levels between age groups were statistically significant (x2=411.17,P＜ 0.01).The reference ranges of normal serum Cys-C levels for different age groups (50-,55-,60-,65-,70-,75-,) were 0.42-0.98mg/L,0.45-1.04mg/L,0.47-1.34mg/L,0.46-1.38mg/L,0.61-1.33mg/L,0.61-1.28 mg/L,respectively,for males,and 0.39-0.94mg/L,0.42-1.01mg/L,0.40-0.91mg/L,0.46-1.03mg/L,0.57-1.04mg/L,0.55-1.27mg/L,respectively,for females.Conclusions This study established preliminary normal serum Cys-C reference ranges for healthy middle-aged and elderly(＞ 50 years)individuals in this region,which can serve as parameters for disease diagnosis and treatment evaluation.

Objective:To investigate the short-term efficacy of thoracoscopic radical surgery in the treatment of esophageal cancer and its influence on the expression of trigger receptor-1 (TRE-1) and tumor necrosis factor receptor-associated protein 1 (TRAP1).Methods:A total of 68 patients with esophageal cancer who were admitted to First People′s Hospital of Ningyang from June 2016 to June 2019 were selected and divided into thoracoscope radical surgery group and raditional surgery group by stratified sampling method, with 34 cases in each group. The thoracoscopic radical surgery group was treated with thoracoscopic radical surgery, and the traditional surgery group was treated with traditional open radical esophageal cancer surgery with neck, chest, and abdominal incisions. The levels of inflammatory factors, immune function, lung function indexes, TREM-1, TRAP1 expression and complications of the two groups were observed and compared.Results:Before operation, the levels of inflammatory factors tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10 in two groups had no significant differences ( P>0.05). At 2 d after operation, the levels of TNF-α, IL-6, IL-10 in two groups were increased and the levels of above index in the thoracoscopic radical surgery group were lower than those in the traditional surgery group: (23.21 ± 0.32) mg/L vs. (29.69 ± 0.48) mg/L, (232.15 ± 23.64) ng/L vs. (246.73 ± 25.89) ng/L, (0.64 ± 0.19) ng/L vs. (0.89 ± 0.21) ng/L, and there were statistical differences ( P<0.05). Before operation, the levels of CD 3+, CD 4+, CD 8+, and CD 4+/CD 8+ in two groups had no significant differences ( P>0.05). At 2 d after operation, the levels of CD 3+, CD 4+, CD 8+ decreased and the level of CD 4+/CD 8+ increased, and the levels of CD 3+, CD 4+, CD 8+, CD 4+/CD 8+ in the thoracoscopic radical surgery group were higher than those in the traditional surgery group: (46.78 ± 1a2.43)% vs. (41.32 ± 9.36)%, (46.12 ± 9.68)% vs. (41.59 ± 7.98)%, (27.42 ± 4.27)% vs. (21.38 ± 3.16)%, 1.47 ± 0.46 vs. 1.25 ± 0.27, and there were statistical differences ( P<0.05). Before operation, the levels of forced expiratory volume in one second (FEV 1), forced vital capacity (FVC), FEV 1/FVC in two groups had no significant differences ( P>0.05). At 2 day after operation, the levels of FEV 1, FVC, FEV 1/FVC in two groups decreased, and the levels of FEV 1, FVC, FEV 1/FVC in the thoracoscopic radical surgery group were higher than those in the traditional surgery group: (2.37 ± 0.72) L vs. (1.82 ± 0.53) L, (3.34 ± 1.06) L vs. (2.43 ± 0.82) L, (62.47 ± 15.26)% vs. (53.67 ± 12.28)%, and there were statistical differences ( P<0.05).Before operation, the expression of TREM-1 and TRAP1 in two groups had no significant differences ( P>0.05). At 2 d after operation, the expression of TREM-1in the thoracoscopic radical surgery group was higher than that of traditional surgery group: (141.56 ± 34.69 vs. 121.54 ± 22.75); the expression of TRAP1 was lower than that of the traditional surgery group: (1.63 ± 0.51 vs. 2.11 ± 0.64), and there were statistical differences ( P<0.05). The postoperative complication rate of the thoracoscopic radical surgery group was lower than that of the traditional surgery group:5.88%(2/34) vs. 23.53%(8/34), and there was statistical difference ( χ2=4.221, P=0.040). Conclusions:The short-term efficacy of thoracoscopic radical surgery in the treatment of esophageal cancer is better than that of the traditional surgery group, which can increase the expression of TREM-1, reduce the expression of TRAP1, and reduce the inflammatory response and the impact on the immune function.