ObjectiveTo study the effects of Pulse Magnetic Needle Apparatus on nerve growth factor(NGF) expression in cerebral ischemia model of rats.Methods70 Wistar rats were divided randomly into 7 groups as normal group,sham group,model group,magnetic group,acupuncture group,elecupuncture group and pulse magnetic group.Cerebral ischemia model was made by middle cerebral artery occlusion.The neural function of all the rats after operation was assessed by Nervous Scale of Function.The expression of NGF in each group was determined using immunohistochemical method.ResultsIn the Nervous Scale of Function between 6h and 15d after operation,there was no difference in model group(P>0.05),but there was difference in magnetic group(P<0.05) and in other therapeutic groups(P<0.01). The curative effect in pulse magnetic group was as the same as that in elecupuncture group and acupuncture group on the 15th day after operation.The expression of NGF increased in all therapeutic groups compared with the model group on the 15th day after operation(P<0.05 or 0.01).Furthermore, the expression of NGF increased in pulse magnetic group and acupuncture group rather than that in magnetic group and elecupuncture group(P<0.05).There was no distinct difference between acupuncture group and pulse magnetic group(P>0.05).ConclusionsThe Pulse Magnetic Needle Apparatus can promote and prolong the expression of NGF and recover the function of limbs.

To evaluate the effects of silicone points polishing on plaque index(PI) and sulcus bleeding index(SBI) after ultrasonic scaling. Forty patients with periodontal diseases were selected for ultrasonic scaling. After scaling,unilateral teeth were randomly assigned for silicone points polishing. PI and SBI were used to evaluate the effects of silicone points polishing. Silicone points polishing significantly reduced PI 12 weeks after scaling. Both in the polishing group and the control group, SBI decreased after scaling. The SBI in the polishing group was significantly lower than that in the control group in the 24 weeks. Silicone points polishing can effectively increase the smoothness of tooth and enhance the effects of uhrosonic scaling.

Many marine steroids with novel structures have been isolated, some of which possess various bioactivities. The new compounds reported through 1996~2000 were reviewed in this article.

Objective To explore the relationship between the intelligence condition and the content of ?-aminobutyric acid (GABA) in cerebrospinal fluid (CSF) in epileptic patients.Methods We measured the Intelligence Quotient (IQ) of 40 cases of epileptic patients by using the Wechsler Intellectual Scaled, and tested their CSF-GABA contents by using HPLC.At the same time, factors which might have influence on patient’s IQ were analysed.Results In the 40 cases of epileptic patients, there were 14 cases of patients with FIQ≤89, and occupied 35%.The intelligence condition of epileptic patients was positive relation with the contents of CSF-GABA, education background and patterns of seizure,but was negative relation with the patient's age. Among all factors, content of CSF-GABA was very significant relationship with VIQ, PIQ and FIQ (P

OBJECTIVE:To evaluate the clinical efficacy of Calcium Hydroxide Iodoform Glycerin(CHIG)paste for root canal disinfection.METHODS:145 patients(145 teeth)requiring root canal therapy were divided randomly into CHIG group,formo-cresol solution group(Group B)and camphor phenol group(Group C).After root canal preparation,the corresponding drugs were introduced into root canal and pulp chamber.The endodontic interappointment pain(EIP)and clinical efficacy in the 3 groups were compared.RESULTS:In Group A,the EIP incidence was the lowest(8.16%)while the effective rate was the highest(97.96%),showing significant differences as compared with Group B(31.91% and 68.09%,respectively)or Group C(34.69% and 71.43%,respectively)(P

Many constituents with novel structures have been isolated from marine brown algae,some of which possess various bioactivities.The terpenoids,lipids,steroids,phlorotannins and some other compounds isolated from brown algae through 1993~2000 were reviewed in this article.

Objective To investigate the influence of Tanyu-Tongzhi (TYTZ)recipe on Rho/Rho kinase signal transduction pathway of myocardial ischemia-reperfusion injury in high fat-fed rats.Methods 60 SD rats were divided into 5 groups randomly,a sham-operated group,a model group,a western medicine control group,a high-dose group and a low-dose group of TYTZ recipe.The model of ischemia reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats.The downstream substrates of ROCK myosin light chain (MLC) phosphorylation expression of myocardial tissues in rats were detected by Western blotting method.The levels of RhoA,ROCK Ⅱ mRNA were determined with RT-PCR.Results Compared with the model group,the expression of p-MLC and RhoA,ROCK Ⅱ mRNA were lower in low-dose group of TYTZ,there were significant differences between the two groups(P=0.004、0.003、0.018、0.004,P＜0.01).Conclusion The TYTZ Recipe can protect myocardium from MI/RI.The mechanism of action was related to its inhibiting the protein expression of RhoA,ROCK Ⅱ and p-MLC,restraining the activation of Rho/Rho kinase signal transduction pathway.

Objective To analyze the relation of hypermethylated secreted frizzled-related protein 2 (SFRP2) gene in feces and peripheral blood of colorectal cancer(CRC) patient with Dukes stages and histological types,and to evaluate SFRP2 gene for screening and judging the pathogenetic condition of CRC.Methmls Methylation-specific PCR assay was performed to analyze SFRP2 gene promoter methylation status in a blinded fashion in tumor tissues,stool and pefipheral blood samples taken from 169 CRC patients,63 patients with adenoma,46 with hyperplastic polyp and 30 endoscopically normal subjects.Results SFRP2 gene Was hypermethylated in 88.2%.65.1%and 45.7% of CRC.adenoma and non-adenomatous polyp tissues,in 84.0%,46.0% and 32.6%of corresponding fecal samples,and in 66.9%,6.4%and 2.2% of corresponding peripheral blood samples,respectively.In contrast,no methylated SFRP2 gene was detected in mucosal tissues and peripheral blood of normal controls,while two cases of matched fecal samples from normal controls were detected with hypermethylated SFRP2.Moreover,no significant correlation were observed between SFRP2 hypermethylation and clinicopathological features including sex,age,size,location and Dukes stage,but there was a significant correlation between SFRP2 hypermethylation and tumor difierentiation and infiltration.SFRP2 hypermethylation in peripheral blood of CRC patients was positively correlated with the Dukes stage. Condusions Hypermethylation of SFRP2 gene in feces works as a molecular biomarker for CRC screening.The hypermethylation of SFRP2 gene is positively related with Dukes stage.

Objective To examine the difference in the effectiveness between proximal femoral nail anti-rotation (PFNA) and femoral head replacement (FHR) of intertrochanteric fracture in elderly patients.Methods This study collected 69 patients that underwent PFNA or FHR after intertrochanteric fractures between June 2012 and September 2014.PFNA group was composed of 41 subjects (17 males and 24 females) aged 80-101 years (mean,86.7 years),and time from injury to operation was (3.12 ± 0.95)days.FHR group was composed of 28 subjects (13 males and 15 females) aged 80-99 years (mean,87.2 years),and time from injury to operation was (3.2 ± 1.0) days.Comparison of operation time,intraoperative blood loss,postoperative blood transfusion,off-bed time,complication incidence,hemoglobin concentration and hip function was made between groups.Results Duration of follow-up was 1-3 years (mean,1.8 years).Operation time and intraoperative blood loss in PFNA group were (46.8 ± 4.4) minutes and (77.0 ± 24.2) ml respectively,differed from (57.4 ± 5.9) minutes and (150.7 ± 46.5) ml in FHR group (P ＜ 0.05).Three days after operation,a similar decrease in hemoglobin concentration was seen in PFNA group [(21.3 ±6.1)g/L] and FHR group [(23.2 ±5.8)g/L] (P ＞ 0.05).Off-bed time in FHR group was (3.2 ± 1.2) days,shorter than (7.1-± 2.5) days in PFNA group (P ＜ 0.05).Incidence of postoperative complications in FHR group was 14％,lower than 37％ in PFNA group (P ＜ 0.05).Hip function of the two groups presented no significant difference six months after operation,but the proportion of excellent results in FHR group was higher than that in PFNA group (82％ vs.73％) (P ＞ 0.05).Conclusion FHR provides better results over PFNA regarding off-bed time,complication incidence and hip function,and hence is preferred for the treatment of elderly patients with intertrochanteric fractures,especially in those with severe osteoporosis.

0.05).Conclusion:People adjust the immunological function of B cells to normal level in blood donation plastochrone.These undoubtedly provide powerfully experimental data on the enlistment of volunter blood donation.