Objective To assess the diagnostic and preoperative classification value of MSCT after administration of water orally and meglumine diatrizoate enema in diagnosis of colonic neoplasms.Methods The day before examination,40 patients with colon carcinoma had a liquid diet and rountine clean enema,then underwent MSCT scan after administration of water orally and 2% meglumine diatrizoate enema.CT findings were analysed in comparison with postoperative pathologic features.Results There were 2 cases of rectal neoplasms,7 cases of sigmoid colon,6 cases of descending colon,5 cases of transeverse colon,and 20 cases of ascending colon cancer.According to Dukes' staging way,the lesions were B stage in 22 cases,C stage in 12 cases and D stage in 6 cases,the rate corresponding to operation was 89.6%.Conclusion MSCT scan after administration of water orally and 2% meglumine diatrizoate enema in diagnosis of colonic neoplasms has great value.

Crystal power/epoxy resin composite was made with refined crystal power as filling material in different composition. The shearing strength and coefficient of thermal expansion of the crystal powder/epoxy resin composite was studied. The sectional morphology was observed under a scanning electron microscope. The results of the study indicate that the shearing strength of crystal powder/epoxy resin composite is improved while the coefficient of thermal expansion decreases with increase of the amount of crystal powder, and a well-proportioned three-dimensional structure is obtained. The augmentation of shearing strength exhibits no linear relationship to the amount of crystal powder, and there is an optimal amount for crystal powder filling. The coefficient of thermal expansion is decreased with increase of crystal powder filling, and three-dimensional structure optimization exhibits the' similar inflexion.

Objective To investigate the impact of down-regulation of histone methyltransferase enhancer of zeste homolog 2 (EZH2) on RUNX3 expressions,proliferation and apoptosis in human pancreatic cancer.Methods The expressions of EZH2 and RUNX3 in 38 pancreatic cancer patients and human pancreatic cancer AsPC-1,PANC-1 and BxPC-3 cells were detected by immunohistochemistry and western blot,respectively.Cells were transfected with siEZH2 by lipofectamin 2000.Real time-PCR and western blot were used to detect EZH2 and RUNX3 expressions.Cell growth and apoptosis in vitro and vivo were assessed by MTT,flow cytometry and nude mice experiments,respectively.The correlation among the expressions of EZH2,clinical pathological features and overall survival rate were analyzed.Results Elevated EZH2 and decreased RUNX3 expressions were observed in human pancreatic cancer tissues and cells (P ＜ 0.05).Knockdown of EZH2 reduced cell growth and induced apoptosis in vitro and vivo by upregulating RUNX3 protein expression (P ＜ 0.05).In addition,the EZH2 expressions were correlated with tumor stage,lymph node metastasis and poor prognosis (P ＜ 0.05).Conclusions EZH2 expressions were correlated with malignancy and poor prognosis in pancreatic cancer.Tumor cell proliferation was promoted by EZH2 through down-regulation of RUNX3.EZH2 may be a potential therapeutic target for pancreatic cancer.

Objective To evaluate the effects of endothelial nitric oxide synthase (eNOS) gene transfer on intrahepatic vascular resistance (IHVR) and portal venous pressure (PVP) in cirrhotic rats. Methods (1) 5 days after eNOS gene transfer, the in situ liver perfusion system (ISLP) was prepared and in different groups of controls and eNOS treated rats, the followings were analyzed: portal perfusion pressure (PP) dose-response curve to norepinephrine (NE); the effects on PP caused by specific nitric oxide synthase (NOS) inhibitor N-monomethyl-L- arginine (L-NMMA) or the nitric oxide (NO) synthesis substrate L-arginine (L-Arg). (2) The experiment of perfusion via portal vein in vivo was performed and the effects of L-NMMA on the PVP was observed. Results (1) In ISLP model, after L-NMMA was added into the perfusate of the control rats, PP dose-respose to NE increased remarkably and the peak of PP increased to (26.7?0.9) mm?Hg. The increased PP response to NE caused by L-NMMA was offsetted by L-Arg and the peak of PP decreased to (23.2?0.9) mm?Hg. In eNOS treated rats, PP response to NE was significantly lower than that in controls (P

Objective To test and evaluate measurement uncertainty and relational performance parameters of the verification device for laser protective spectacles.Methods By using realtime beam-splitting surveillance,several performance parameters,including the measurement repeatability of the verification device,optical density of laser protective spectacles in different laser exposure conditions and its damage threshold,were tested.The measurement modules of the main performance parameters were built up as the experiment & technology platform for performance evaluation of laser protective spectacles and for measurement uncertainty appraisal of verification device.Results Laser wavelengths of the verification device were 1064nm and 532nm,response range of wavelength was from 0.4?m to 1.1?m,measuring range of laser energy was from 10-8 J to 0.3J,measuring range of optical density for laser protective spectacles was from 0.1 to 8.0,measuring repeatability was 0.37%,and measuring uncertainty was 5% (k =2).Conclusion The automatic verification device is steady and reliable.The achieved performance indexes accord with the designed requirement for laser protection.

Malaria vaccines are being developed against different stages in the parasite's life cycle. Although not directly protective ,the sexual stage vaccines would induce antibodies that would prevent infection of mosquitoes when ingestedin a bloodmeal containing sexual stage parasites. pfs25 has been tested to be an important candidate antigen for malarial transmission blocking vaccine . In this report we analyzed the complete code of pfs25 gene of Plasmodium falciparum PFD-3/YN isolate. The result shows that the gene encoding pfs25 of PED-3/YN isolate has a mutant which generates a PstI endonuclease restriction site and shares 99.2％ nucleotide homology with that of NF4 isolate.

Objective: To study the mRNA and protein expressions of ICAM 1 and CD44s in non small cell lung cancer as well as nearby normal lung tissue. Methods: RT PCR and SP immunohistochemistry were used to detect the mRNA and protein expressions of ICAM 1 and CD44s in 42 non small cell lung cancer and nearby normal lung tissue. Results: The expression level of ICAM 1 and CD44s increased in NSCLC tissue than in normal tissue. No correlations were noted in expression of ICAM 1 and CD44s and pathological type, histological differentiation and TNM stage. But there was significant correlation between abnormal expressions of ICAM 1 and CD44s and lymph node metastasis. Conclusion: These results suggest that the level of ICAM 1 and CD44s genes expression in lung cells increases as they progress from normal to the transformed stage, that indicate these two genes are involved in the process of lung cancer development.

Objective To analyze the common causes leading to lung infection following renal transplantation and provide targeted preventive measures to reduce the incidence of lung infection.Methods The clinical data of 561 recipients who underwent renal transplantation from January 2006 to February 2011 were retrospectively analyzed.The recipients were divided into two groups:group Ⅰ,from January 2006 to December 2009 (n =416) ; group Ⅱ,from January 2010 to February 2011 (n =145).The causes possibly leading to lung infection which took place 3 days before the appearance of the clinical symptoms were offered by the patients who suffered lung infection of group Ⅰ.And then the causes were summarized and analyzed to formulate the specific and comprehensive measures to prevent the infection.Finally the measures were applied to recipients in group Ⅱ from January 2010.After applying the measures for 14 months,the incidence of lung infection in group Ⅱ was counted and compared with that in group Ⅰ to see the preventive effect.Results There were 58 cases of lung infection in group Ⅰ (58/416,13.9％) and 12 cases in group Ⅱ (12/145,8.3％). There was significant difference in the incidence of lung infection between two groups (x2 =4.0361,P＜0.05).All of the recipients with lung infection were hospitalized in six months after the transplantation.The causes leading to lung infection of 58 cases in group Ⅰ were as follows:6 cases due to being excessively tired,3 cases due to guest visiting,12 cases due to abrupt change of weather,9 cases due to exposure to public place,8 cases due to returning to hospital,6 cases due to close contact with children,5 cases due to close contact with animals,and the other 9 cases without specific causes found.Conclusion The incidence of lung infection following renal transplantation can be notably reduced by the application of targeted and concrete health propaganda education and preventive measures based on analysis on the specific causes of infection.

Objective Based on the principles of laser radiation protection, medical metroiogy and photoelectron technology, an automatic verification device and testing technology to provide verification and performance evaluation for laser protective spectacles and equipments which have the various functions in laser protection have been developed and established. Methods The current system comprises laser source, laser measuring instruments, software of computer detection and control and modules of optics and mechanics used in auxiliary equipment. By use of the verification device and the special test-recording method to be designed and studied by us, the measured data can be automatically acquired, processed, recorded, saved and printed and, furthermore, many parameters on protective performance of the measured laser protective spectacles can be tested and given. Results Laser wavelength of the verification apparatus are 1 064 nm and 532 nm, response range of wavelength is from 0.4 m to 1.1 μm, measuring range of laser energy is from 10-8 J to 0.3 J, measuring range of optical density for laser protective spectacles is from 0.1 to 8.0, stability is 0.21%, measuring uncertainty is 5%(k=2). Conclusion The automatic verification device is steady and reliable. The achieved performance indexes accord with the requirement of national standard on laser protection.

Objective To investigate the alteration of chaperone hsp40 and its effects on the dealyed neuron death in the CAI neurons after transient cerebral ischemia.Method Twenty-minute transient global ischemia rat model was used.Following different repeffusion period,all the 28 wistar rats were divided into sham-operation group ,4-hour recovery group,24-honr recovery group and 72-hour recovery gronp,7 ratsin in each group,Immunochemistry and laser scanning confocal microscopy were used to observe the distributional alteration of hsp40 in the neurons.Differential centrifuge and westemblot analysis were used to analyze the quantitative alteration of hsp40 and its redistribution in the neurons.Results lnanunechemistry and laser scanning confocal microscopy showedthe reduction of hsp40 first in cytosol,then in the nucleus until all the neurons in the CAI region died.Differential centrifuge and westemblot analysis showed the quantity of hsp40 decreased from (1.00_+0.21) to (0.23±0.13)(P<0.01) after 24-hour repeffusion;the quantity of hsp40 in the protein aggregates increased from (1.00±0.18) to(8.61±1.89)(P<0.01) after24-hour reperfusion.Conclusions The reduction of hsp40 in the neurons of hippocampus CA1 region is an important factor resulting in protein aggregates formation.