1.Puerarin Protects the Glaucomatous Retina against the Damage Induced by Oxidative Stress
Tianjin Medical Journal 2013;(12):1199-1201
Objective To investigate the role of puerarin in protecting the glaucomatous retina against the damage induced by oxidative stress. Methods Twenty-four healthy adult male rabbits were divided into three groups: control group, model group and therapy group with 8 rabbits per group. The glaucoma model rats of therapy group and model group were induced by injecting 2.5%hydroxypropyl methylcellulose into the anterior chamber of rabbits. The therapy group was treated by injecting puerarin into the peritoneal of rabbits (10 mL/kg,one time a day for 21 days). The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), the content and the redox state of glutathione and the contents of malondialdehyde (MDA) and nitric oxide (NO) in the retina were measured at the fourth week after the induc-tion of glaucoma by using corresponding kits. Results The activities of SOD, GSH-Px and CAT, the content of reduced glu-tathione (GSH) and the ratio of GSH/oxidized glutathione (GSSG) in retina were lower in model group than those of control group. The contents of GSSG, MDA and NO were higher in model group than those of control group. The activities of SOD, CAT and GSH-Px, the content of GSH and the ratio of GSH/GSSG in retina were higher in therapy group than those of model group, but still lower than those of control group. The contents of GSSG, MDA and NO were lower in therapy group than those of model group. There was no statistical difference in the content of GSSG between therapy group and control group. Conclu-sion Puerarin can enhance the activities of antioxidant enzymes and maintain the redox state of glutathione, which, in turn, alleviates the injury of the glaucomatous retina induced by oxidative stress.
2.Evaluation of Clinical Effect of Serum FT_3,FT_4 in Patients With Liver Cirrhosis
Journal of Chinese Physician 2001;0(08):-
Objective To study the relationship between changes of contents of serum free-triiodothyronine(FT 3),Free-thyroxine(FT 4) and liver cirrhosis,and analyse it's clinical significanec.Methods The concentration of serum FT 3 and FT 4 were determined by radioimmunoassay in 69 patients with liver cirrhosis,compared with liver function classification(Child-Pugh).Results Contents of serum FT 3,FT 4 in cirrhosis patients were markedly lower than that of controls(P
3.Stellate ganglion block in the treatment of breast cancer with tidal fever:24 cases of clinical report
Chinese Journal of Primary Medicine and Pharmacy 2016;23(10):1517-1519
Objective To provide application stellate ganglion block treatment of hot flashes′latest data by clinical.Methods 24 patients who were put in the research were taken record of each patient after stellate ganglion block when two months later,especially of the clinical symptoms and hot -flashes -onset,and they were asked to fill a symptom questionnaire for each one.The main concern of hot flashes in patients with seizure frequency of hot flashes and score changes were observed.The data were analyzed.Results After the implementation of stellate ganglion block,the number of episodes of hot flashes in breast cancer patients,and ratings declined,and after 6 weeks decreased by 46% and 49%.21 patients observed in Horner's syndrome.Conclusion Stellate ganglion block for the control of hot flashes in breast cancer is effective.
4.Semen cassiae extract attenuates myocardial ischemia and reperfusion injury in type 2 diabetic rats
Basic & Clinical Medicine 2015;(1):1-6
Objective Obese patients with type 2 diabetes mellitus ( T2DM) characterized by hyperglycemia are li-able to more severe myocardial infarction .Semen Cassiae is proved to reduce serum lipid level .This study was to investigate whether the Semen Cassiae extract (SCE) reduces myocardial ischemia and reperfusion (MI/R) injury with or without diabetes and the underlying mechanisms .Methods The high-fat diet-fed streptozotocin ( HFD-STZ) rat model was used as T2DM model.Normal and DM rats received SCE treatment orally (10 mg/kg/day) for 1 week.Subsequently these animals were subjected to MI /R.Results Compared with the normal animals , DM rats showed increased plasma total cholesterol ( TC) and triacylglycerol ( TG) , more severe MI/R injury and cardi-ac functional impairment .SCE treatment significantly reduced the plasma TC and TG , improved the instantaneous first derivation of left ventricle pressure and reduced infarct size , decreased plasma creatine kinase and lactate dehydrogenase levels, and reduced apoptosis index at the end of reperfusion in diabetic rats .Moreover, SCE treat-ment increased the antiapoptotic protein Akt and stimulated ERK 1/2 phosphorylation .Pretreatment with a PI3 K in-hibitor wortmannin or an ERK1/2 inhibitor PD98059 not only blocked Akt and ERK1/2 phosphorylation, but also inhibited the cardioprotective effects of SCE .However , SCE treatment did not show any effects on the MI/R injury in the normal rats.Conclusions SCE effectively improves myocardial function and reduces MI/R-induced injury in diabetic but not normal animals , which is potentially attributable to the reduced TC/TG levels and the triggered cell survival signaling Akt and ERK 1/2 .
5.The application of stellate ganglion block in treatment of seroma afar modified radical mastectomy of breast cancer
Chinese Journal of Primary Medicine and Pharmacy 2010;17(12):1609-1610
Objective To evaluate the effects of stellate ganglion block(SGB) in treating seroma after modi-fled radical mastectomy of breast cancer.Methods 76 patients were treated with SGB besides local compressed bind-ing and were evaluated after ten days.Results There were ten seroma with the incidence rate of 13.2% and no skin flap necrosis.Conclusions SGB combined with local compressed binding could treat secroma after modified radical mastectomy of breast cancer.
6.Radiotherapy efficacy of E-Flow imaging on cervical lymph nodes metastasized from nasopharyngeal carcinoma
Journal of Central South University(Medical Sciences) 2010;35(6):566-571
Objective To explore the effect of E-Flow imaging on the radiotherapy of cervical lymph nodes metastasized from nasopharyngeal carcinoma.Methods A total of 52 nasopharyngeal carcinoma patients with 94 invasion neck lymph nodes were treated with conventional radiotherapy. Lymph node size, hemodynamic parameters, and blood supply were detected by E-Flow imaging and two-dimensional ultrasound before and after the radiotherapy. The relationship between those examinations and radiotherapy effect was analyzed.Results Of the 94 metastatic cervical lymph nodes from nasopharyngeal carcinoma before the radiotherapy, 40 disappeared, 52 reduced, and 2 maintained the original size after the radiotherapy. Blood flow in all residual lymph nodes decreased or disappearaed. Artery flow spectrum in the lymph node was changed from the high-speed high impedance to low-speed low resistance. The size of cervical lymph node and blood flow before the radiotherapy were associated with clinical efficacy (P<0.05). Conclusion E-Flow imaging plays an important role in assessing the efficacy of radiation therapy by changing the blood supply of cervical lymph node and its size before and after the radiotherapy.
7.Advances of endoscopy in the diagnosis and treatment of colorectal polyps in children
Journal of Clinical Pediatrics 2014;(4):301-303
Colorectal polyps are common digestive system diseases in children. The important role of endoscopy in the di-agnosis and treatment of colorectal polyps in children has been recognized. With the continuous development of endoscopic techniques, the diagnosis and treatment of colorectal polyp has become safer, more convenient and more effective. In this re-view, the progress in endoscopy for diagnosis and treatment of colorectal polyps in children has been summarized.
8.Distribution and antimicrobial resistance of pathogens isolated from bone and joint infection sites of orthopedic patients
Chinese Journal of Infection Control 2015;(1):60-62
Objective To understand the distribution and antimicrobial resistance of pathogens isolated from bone and joint infection sites of orthopedic patients,so as to provide reference for prevention and therapy of bone and joint infection.Methods 114 specimens from bone and joint infection sites of orthopedic patients between June 2010 and October 2013 were surveyed,pathogens and antimicrobial susceptibility of pathogens were analyzed.Results A total of 145 pathogenic isolates were obtained,62.07%(n=90)of which were gram-negative bacteria,34.48%(n=50)were gram-positive bacteria,2.07%(n=3)were Candida spp .,and 1 .38%(n =2)were anaerobic bacteria. The top 4 pathogens were Pseudomonas aeruginosa (20.69%),Staphylococcus aureus (12.41 %),Escherichia coli (11 .03 %),and Enterobacter cloacae (9.65%).These pathogens presented remarkable resistance.The resistant rate of Pseudomonas aeruginosa to ceftazidime was up to 40.00%;the resistant rates of Escherichia coli to com-pound sulfamethoxazole,ciprofloxacin and levofloxacin were all 100.00%.Conclusion Gram-negative bacteria are the major pathogens in orthopedic ward,the isolated strains are resistant to multiple antimicrobial agents.
9.Multivariate analysis of the relationship between free DNA concentration in the blood and prognosis of non-small cell lung cancer
Clinical Medicine of China 2013;29(10):1032-1035
Objective To investigate the association of circulating free blood DNA and prognosis of non-small cell lung cancer (NSCLC).Methods Forty-six untreated NSCLC patients and 21 controls were selected as our subjects,and all underwent a 6 years follow-up.The level of circulating free blood DNA was determined by real-time quantitative polymerase chain reaction (qPCR) targeting the human β-globin gene.Overall survival of the patients were analyzed by Kaplan-Meier method and Cox-regression.Results At the end of follow-up,the median free DNA concentration of experimental patients was higher than that of control patients(52 μg/L vs.29 μg/L,P =0.03).The median free DNA concentration of experimental death patients (87%) died was significantly higher than that of still survived patients (59 mg/L vs.25 mg/L,P =0.02).No association of DNA concentration with tumor pathology,clinical stage,age,gender,smoking or pulmonary inflammatory conditions were found in current study.Conclusion For NSCLC patients,the circulating free blood DNA level was negative correlation with the survival of the patients,suggesting that it may be utilized as a prognostic factor for survival analysis.But there is no relationship of free blood DNA concentration with tumor pathology,age,gender,smoking or pulmonary inflammatory conditions.
10.Application of Transverse Fascia in Inguinal Hernia Repair
Chinese Journal of Bases and Clinics in General Surgery 2008;0(07):-
0.05).The differences of severe postoperative pain,testicular swelling,the time of the body’s restore for normal activities,and recurrence rate of patients between two groups were significant (P0.05).Conclusion The strengthening of posterior wall by transverse fascia and reconstruction of inner ring is a simple and effective method for inguinal hernia repair.