TROP2 (trophoblastic cell surface protein 2),a cell-surface glycoprotein,is named after its high expression only in trophoblast cells.Recent studies found that TROP2 protein is overexpressed in a variety of human epithelial cancers,and it is associated with the prognosis.However,the mechanism is still not clear.

To evaluate the effects of silicone points polishing on plaque index(PI) and sulcus bleeding index(SBI) after ultrasonic scaling. Forty patients with periodontal diseases were selected for ultrasonic scaling. After scaling,unilateral teeth were randomly assigned for silicone points polishing. PI and SBI were used to evaluate the effects of silicone points polishing. Silicone points polishing significantly reduced PI 12 weeks after scaling. Both in the polishing group and the control group, SBI decreased after scaling. The SBI in the polishing group was significantly lower than that in the control group in the 24 weeks. Silicone points polishing can effectively increase the smoothness of tooth and enhance the effects of uhrosonic scaling.

Objective: To observe the synergetic effect of folic acid and genistein on apoptosis in the early stage of neuron and its possible mechanism. Methods: Cyclophosphamide was added to primary cultured neurons to induce apoptosis. Three different dosages of folic acid and genistein were used to inhibit apoptosis. The results were observed by flow cytometry and scanning electron microscopy (SEM). Results: 1.Folic acid and genistein might play a role in early stages of neuron apoptosis, and the synergetic effect was demonstrated when folic acid and genistein were administered together. 2. The membrane changes in early stages of neuron apoptosis observed by scanning electron microscope were also in agreement with the flow cytometry data. Conclusion: Genistein might reinforce the protective effect of folic acid on neural tube defects by restraining the apoptosis of the early stage of neuron.

OBJECTIVE:To evaluate the clinical efficacy of Calcium Hydroxide Iodoform Glycerin(CHIG)paste for root canal disinfection.METHODS:145 patients(145 teeth)requiring root canal therapy were divided randomly into CHIG group,formo-cresol solution group(Group B)and camphor phenol group(Group C).After root canal preparation,the corresponding drugs were introduced into root canal and pulp chamber.The endodontic interappointment pain(EIP)and clinical efficacy in the 3 groups were compared.RESULTS:In Group A,the EIP incidence was the lowest(8.16%)while the effective rate was the highest(97.96%),showing significant differences as compared with Group B(31.91% and 68.09%,respectively)or Group C(34.69% and 71.43%,respectively)(P

Sternal tumors are uncommon, mostly malignant.Radical resection can offer a definitive cure of these tumors. Improvement of skeletal and soft tissue reconstruction have made coverage of sternal defects reliable .

Objective:To evaluate the clinical value of activated carbon nanoparticles for guiding lymphadenectomy in advanc-ing rectal cancer. Methods:Eighty rectal cancer patients who underwent laparoscopic curative resection for rectal cancer were divided into two groups:control group (40 cases) and experiment group (40 cases). The experiment group received carbon nanoparticle-labeled lymph nodes in surgery. The number of lymph nodes, lymph nodes≤5 mm in size, and positive lymph nodes, as well as the side effect of the procedure, were analyzed. Results:No complications were observed in the experiment group. The experiment group showed sig-nificantly higher values (P<0.05) than the control group for average number of lymph nodes (25.5 ± 8.78 vs. 16.05 ± 4.84), lymph nodes≤5mm in size (22.6 ± 8.25 vs. 13.65 ± 4.62), and positive lymph nodes (3.13 ± 4.14 vs. 1.35 ± 2.06). During operation, two dyed lymph nodes in two cases were found at the roof of the inferior mesenteric artery and along the side of the internal iliac artery. Dissec-tion was extended for these patients and the dyed lymph nodes were confirmed to be positive. Conclusion:Local injection of activated carbon nanoparticles around the tumor during surgical exploration was an effective, secure, and easy approach for guiding lymphade-nectomy in rectal cancer patients.

Objective To investigate the expression of Oct4 protein and analyze its correlation with the clinic pathological features and prognosis of non-muscle-invasive bladder cancer.Methods The oct4 protein expression was assessed by immunohistochemical analysis in 87 specimens of bladder transitional cell carcinoma and 15 specimens of adjacent normal tissues.A correlation between Oct4 and clinic pathological features was analyzed.Results The positive rate of Oct4 protein was significantly higher in bladder cancer than that in normal bladder tissue (P＜0.01).The positive rate of Oct4 protein was 40.7％ in G1 bladder cancer,69.4％ in G2 bladder cancer and 91.7％ in G3 bladder cancer,and the differences was significant (P＜0.01).All patients were followed up for 3-78 months,and 63 of them relapsed.The expression of Oct4 protein was significantly higher in patients of recurrence than in non-recurrence (77.8％ ∶ 37.5％,P ＜ 0.01 ).21 patients of recurrence were in progression,and the expression of Oct4 protein had no significant differences between patients of progression and non-progression (71.4％ ∶65.2％,P ＞0.05).The positive rate of Oct4 protein was not related with gender,age,tumor number and size (P ＞0.05).Conclusion The detection of Oct4 protein is in favor of early detection of bladder tunor,estimation the degree of differentiation and surveillance for recurrence of superficial bladder cancer.

Objective To evaluate the efficacy and security of one-stop hybrid cardiac surgery for the treatment of elderly patients with complex heart disease.Methods From November 2013 to March 2014,a total of 5 patients[4 male,age:71-78years] underwent one-stop hybrid approach in the hybrid operating room with chronic obstructive pulmonary disease (COPD) in 3 cases,2 cases of diabetes,3 cases of hypertension,valvular heart disease in 3 cases,PDA in 1 case,4 patients with multivessel coronary disease.Right minimally invasive cardiac surgery(coronary artery bypass grafting or valvular surgery) and percutaneous intervention were performed in an enhanced operative unit.The efficacy and security of one-stop hybrid cardiac surgery were evaluated after the procedure.Results The one-stop hybrid procedure was successful in all patients.Operation time was (125 ± 28) minutes,CPI time was (65 ± 33) minutes,CPB time was (60 ± 23) minutes,Aortic cross clamping time was (42 ± 18) minutes,postoperative mechanical ventilation time was (20.5 ±6.5)hours and ICU monitoring time was (68.9 ± 16.6)hours.Hospitalization time was (9.8 ± 3.7) days.Patients remained free from angina,prosthetic valve dysfunction and patent ductus arteriosus recanalisation(rang 1 to 5 months) follow-up period.Conclusion One-stop hybrid cardiac surgery provides a reasonable,feasible and safe alternative for treating elderly patients with complex heart disease.

The methyl group plays an important role in the rational drug design. Introducing methyl into small molecules has become an important strategy of lead compound optimization. The application of methyl in drug design is reviewed in this paper. Methyl can modulate the physicochemical, pharmacodynamic, and pharmacokinetic properties by ortho effect, inductive effect, and conformational effect. It also improves the metabolic stability as a soft metabolic point. In addition, introducing methyl into drug molecules can also be applied as a strategy in new uses of old drugs and generate me-too drugs.

Objective:To evaluate the clinical effects of oncoplastic breast-conserving surgery on patients with early breast can-cer near the mammary areola. Methods:A total of 60 patients with early breast cancer underwent breast-conserving surgery in the Sec-ond Department of Breast Surgery, Tangshan People's Hospital from February 2011 to November 2013. These patients were random-ized into two groups, namely, the experimental Group A (n=30) and the control Group B (n=30). Oncoplastic breast-conserving surgery was performed on the patients in Group A, whereas Group B underwent standard breast-conserving surgery. The specimen weight of the locally excised breast, the nearest distance of the tumor to the surgical margins, and the postoperative cosmetic result of the affected breast were compared between the two groups. Results: The specimen weights of the locally excised breast were 71.03 ± 12.92 and 41.53±7.13 g, and the nearest distances of the tumor to the surgical margins were 13.30±2.97 and 10.63±1.65 mm in Groups A and B, respectively, with significant differences between the two groups (P<0.05). The postoperative satisfaction rates of the affected breast were 93.33%and 83.33%in Groups A and B, respectively, without any significant differences between the two groups (P>0.05). Con-clusion:A larger amount of excised breast tissue and a wider scope of surgical margins were observed in Group A patients. However, the postoperative cosmetic result of the affected breast was almost similar for both groups. Therefore, oncoplastic breast-conserving sur-gery is a feasible and effective approach for early breast cancer patients.