AIM Inhibitors of xanthine oxidase (XOD) may be potentially useful for the treatment of gout or other XOD inducing diseases, so the inhibition on XOD activity of diterpenoid tanshinones such as cyptotanshinone (CT) and methylenetanshinone (MT) from Tanshen (Salvia miltiorrhiza Bge.) was studied. METHODS The formation rate of uric acid from xanthine was determined by measuring the absorbance increment at 290 nm (ΔA290 nm) in the reactive medium of xanthine/XOD when CT or MT was added. RESULTS It was found that CT and MT inhibited XOD activity. Dixon plots showed that the inhibition mode was competitive type. The Ki values of CT and MT were 17.8 μmol·L-1 and 25.9 μmol·L-1, respectively. Their inhibitory potencies positively correlated with their concentrations and the IC50 values of CT and MT were 70 and 67 μmol·L-1, respectively. The IC50 value of allopurinol, the positive control, was 60 μmol·L-1. CONCLUSION CT and MT have inhibitory effects on XOD activity and may be potentially useful for the treatment of gout or other XOD inducing diseases.

Objective: To study the antioxidative and antilipemic effect of the flavonoids purified from pomelo (Citrus grandis L.Osbeck) peel. Methods: The flavonoids were isolated and purified by NKA-12 macroporous adsorption resin and the polyamide column chromatography, and preparative TLC and HPLC. DPPH radical scavenging potential and inhibition of human LDL oxidation in vitro were determined. The effects TG , TC, HDL-C and LDL-C were investigated in mice with hypercholesterolemia induced by egg yolk. Results: Three kinds of flavonoids antioxidants named P11, P12 and P2, deduced as hesperidin, naringin and eriocitrin, were purified from pomelo peel. The radical scavenging potential of P2, P11 and P12 were 52.5%, 37.8% and 39.0% respectively (P

Objective:To investigate the safety and effect of complete mesocolie excision (CME) combined with arterial infusion chemotherapy (AIC) and intra-peritoneal interstitial sustained-release chemotherapy (IPISRC). Methods:A total of 104 patients were classified under the experimental group and underwent CME combined with AIC and IPISRC. The other 98 patients were classified un-der the control group and only received radical surgery. Pre-and post-operative blood routine examinations, as well as liver and kidney function tests, were conducted for both groups. Post-operative adverse reactions and incidence of complications were recorded. Cancer and para-neoplastic tissues were sampled in experimental group. The post-surgery 5-fluorouracil (5-FU) concentration in the drainage fluid as well as those in the peripheral blood , were determined. Three-year follow-ups were conducted, during which the local recur-rence rate, liver metastasis, progression-free survival rate, and total survival rate were recorded. Results: No significant differences were found in the white blood cell count, hemoglobin count, liver and renal functions of the patients before and after the surgery, and rate of adverse reaction and complications between the two groups after surgery (P>0.05). In experimental group , the 5-FU concentra-tion was significantly higher in the cancer tissues than in the para-neoplastic tissues . The 5-FU concentration in experimental group was also significantly higher in the intra-peritoneal drainage liquid and reached its peak in the peripheral blood on day 3 post-surgery . Local recurrence and liver metastasis rates were significantly lower in experimental group than those in control group, whereas the pro-gression-free and three-year overall survival rates were significantly higher in experimental group than in control group (P<0.05). Con-clusion:The tharepy of pations of experimental goup is safe and effective. This method significantly improves the progression-free and three-year survival rates of the patients as well as significantly reduces the local recurrence and liver metastasis rates of colon cancer.