Objective To explore the relation between serum AMH,IGF-1 and IGFBP-1 levels and polycystic ovarian syndrome (PCOS) and its clinical significance.Methods Serum levels of AMH,IGF-1 and IGFBP-1 were measured by ELISA in 80 patients with PCOS and 80 healthy subjects.Results The levels of serum AMH,IGF-1,IGFBP-1 in PCOS group were (7.18 ± 1.50) ng/L,(200.85 ± 27.78) ng/L,(4.05 ± 0.65) ng/L respectively,which in the control group were (3.70 ± 1.01) ng/L,(110.58 ± 12.50) ng/L,(8.16 ± 1.39) ng/L.The serum levels of AMH,IGF-1 in the PCOS group were significandy higher than those in the control group(t =13.14,26.80,P ＜ 0.01),while the serum level of IGFBP-1 in the PCOS group was significantly lower than that in the control group(t =16.28,P ＜0.01).The serum levels of AMH,IGF-1,IGFBP-1 in patients with obese group were (7.18 ± 1.50) ng/L,(200.85 ± 27.78) rng/L,(4.05 ± 0.65)ng/ L respectively,which in the non-obese group were (15.52 ± 2.41) ng/L,(143.85 ± 15.61) ng/L,(6.75 ± 0.93) ng/L.The serum level of IGF-1 in the obese group was significantly higher than that in the non-obese group(t =10.87,P ＜0.01),while the serum levels of AMH and IGFBP-1 in the obese group were significandy lower than those in the non-obese group(t =18.98,15.27,P ＜0.01).Conclusion Serum AMH,IGF-1 and IGFBP-1 may be involved in the pathogenesis and obesity of PCOS,which may be associated with the pathogenesis of PCOS.

Objective To explore the changes of Tr cells and IL-17 levels in peripheral blood of patients with antiphospholipid syndrome (APS) and the clinical significance.Methods The quantity of the CD4+ CD25+ Tr cells and proportion of CD4+ CD25+ Tr cells/CD4+ T cells were detected by flow cytomety,and the levels of IL-17 were detected by ELISA in 60 cases with APS(APS group) and 60 healthy people(control group).Results The levels of Tr,Tr/CD4+,Foxp + 3 Tr/Tr in the APS group were (3.03 ± 0.40) ％,(0.11 ± 0.02),(0.40 ± 0.10),respectively,which in the control group were (5.76 ± 0.84)％,(0.15 ± 0.03),(0.73 ± 0.15),respectively,the differences were statistically significant(t =22.73,8.59,14.18,all P ＜ 0.01).The levels of serum IL-17 and IL-17/Tr cells in APS group were (15.31 ±2.00)pg/mL,(5.05 ±0.29)pg/mL,respectively,which in the control group were (6.63 ± 1.52) pg/mL,(1.15 ± 0.14) pg/mL,respectively,there were statistically significant differences (t =29.17,93.81,all P ＜0.05).The levels of serum IL-17 was negatively correlated with Tr cells level in peripheral blood of patients with APS(r =-0.801,P ＜0.01).Conclusion There is peripheral blood cellular immune function disorder in APS patients,the number and active changes of CD4+ CD25+ Tr cells and IL-17/Tr ratio imbalance may play a role in the pathogenesis of APS.

Objective To investigate the effect and mechanism of quercetin in LPS-induced acute lung injury. Methods The PaO2, PaCO2, RR and pH, the expression of IL-6、 IL-1β、 TNF-α、 JAK2、 STAT3、 p-JAK2、 p-STAT3, the pathological change and Dry/Wet of Lung tissues were evaluated in rats. Results Quercetin can significantly decrease the pathological change of Lung tissues, the PaCO2, RR and the expression of IL-6、IL-1β、 TNF-α、 p-JAK2 and p-STAT3 with increasing the PaO2, pH and D/W. Therefore, with on influence on the expression of JAK2. Conclusion Quercetin pretreatment decreased LPS-induced acute lung injury by suppressing JAK2/STATA3 signallingto improve exchange ability though reducing inflammation.

OBJECTIVE To use ultrasound technique to rinse the medical apparatus and raise the rinsing quality of apparatus.METHODS Under the magnifier to examine the cleanliness of the apparatus.According to pyrogen test by standard Limulus lysate,to verify the ultrasound rinsing effect by pyrogen removing.RESULTS It was very clean when rinsing the apparatus and with no blind area to uneven parts and articular ones.Monitoring result of pyrogen was negative through rinsing the syringe by ultrasound.CONCLUSIONS The rinsing quality of apparatus is improved.

OBJECTIVE:To determine plasma concentration of caffeine,dapsone and chlorzoxazone in rats,and to calculate pharmacokinetic parameters. METHODS:6 rats were given the mixture of caffeine,dapsone and chlorzoxazone intragastrically, 1.5,2 and 3 mg/kg,respectively. 0.2-0.3 ml blood were collected before medication and 0.5,1,2,3,4,6,8,12,24 h after medication.The plasma sample was treated with solid phase extraction. The plasma concentration of caffeine,dapsone and chlorzoxa-zone were determined by HPLC using N-(2-Hydroxyethyl) phthalimide as internal standard. The pharmacokinetic parameters were calculated using DAS 2.0 software. RESULTS:The linear ranges of caffeine,dapsone and chlorzoxazone were all 0.2-30 μg/ml (r were 0.996 4,0.996 1,0.998 8,respectively). The limit of quantitation were 0.2 μg/ml. The recoveries of low-concentration, medium-concentration and high concentration were(84.8±3.6)%-(111.4±10.2)%(RSD were 4.3%-9.8%,n=3),(107.0±13.3)%-(113.5±8.1)%(RSD were 7.1%-14.0%,n=3),(104.2±10.8)%-(111.1±12.2)%(RSD were 8.0%-11.0%,n=3). Pharmacoki-netic parameters were as follows as tmax(1.70±0.99),(1.50±1.00),(1.92±0.80)h;t1/2(0.73±0.22),(2.77±1.35),(2.78±2.34) h;cmax (2.60 ± 0.50),(5.78 ± 1.19),(9.76 ± 1.37) mg/L;AUC0-t (8.43 ± 0.79),(20.68 ± 1.91),(26.71 ± 2.45) mg·h/L(n=6). CONCLUSIONS:The method is simple,sensitive and accurate,and can be used for the plasma concentration determination and pharmacokinetic study of caffeine,dapson and chlorzoxazone.

Objective To evaluate the recent safety and long-term curative effect of double-pool shaping trabeculectomy with adjustable sutures in glaucoma. Methods A hundred and twenty patients with glaucoma were randomly divided into two groups, in which the control group in 62 patients underwent traditional trabeculectomy and the experimental group in 58 patients underwent double-pool shaping trabeculectomy with adjustable sutures. All patients were followed up for one year. Postoprative intraocular pressure, anterior chamber depth and filtering bleb were observed. Results In the experimental group, after the demolition of adjustable sutures, 3 eyes(5.17%) suffered from I degree shallow anterior chamber. By contrast, 8 eyes suffered from I degree shallow anterior chamber, and 1 eye (total 14.52%) suffered from Ⅱ degree shallow anterior chamber in the control group (P < 0.05). Following up in one year, the functional filtering bleb and intraocular pressure got better in the experimental group than in the control group (P < 0.05). Conclusion Double-pool shaping trabeculectomy with adjustable sutures is a safe and feasible method for glaucoma, in which it can effectively reduce intraocular pressure without complications.

Objective To evaluate the clinical value and safety of hysteroscopy in diagnosis of abnormal uterine bleeding.Methods The clinical characters and histopathological data of 272 cases of abnormal uterine bleeding were retrospectively analyzed.They received hysteroscopy in our hospital from Jan.2010 to Jan.2015.Results Histopathological diagnosis showed 75 cases were with endometrial polyps,including 5 cases misdiagnosed as submucosal fibroids,and the correct diagnostic rate was 93.3％ (70/75).50 cases were with submucosal fibroids,and the diagnostic rate was 90.9％ (50/55).97 cases were endometrial hyperplasia,including 7 cases misdiagnosed as atypical hyperplasia or cancer by hysteroscopy,and the correct diagnostic rate was 92.8％ (90/97).19 cases were atypical hyperplasia or endometrial carcinoma,and the diagnostic rate was 73.1％(19/26).Atrophic endometrium were 23 cases.8 cases had intrauterine device remained.Conclusions Premenopausal women are more likely to present with endometrial hyperplasia,submucosal fibroids,endometrial polyps,while endometrial cancer and precancerous lesions are rare.But in postmenopausal women endometrial polyps,atrophic endometrium,cancer and precancerous lesions are mainly included.Hysteroscopic has a good diagnostic value in endometrial diseases which should be combined with histopathological examination.

Forty-eight male Wistar rats,weighing 280 to 320 g,were selected and prepared by fasting but free drinking for 12 hours before the experiment.They were randomly divided into four groups by random number table (12 rats in each group):control group,ischemic group,reperfusion group and propofol group.Control group:normal saline infusion[10 ml /(kg?h)]for 1 h in caudal vein without any other treatment.Ischemic group:on the basis of control group,supply with 20 mmHg(1 mmHg =0.133 kPa)pneumoperitoneum pressure for 1 h. Reperfusion group:on the basis of ischemic group,keep pneumoperitoneum pressure open for 30 min.Propo-fol group:infusion propofol in caudal vein [10 ml /(kg?h),saline 10-fold dilution],given 20 mmHg pneu-moperitoneum pressure for 1 h,then kept this pressure open for 30 min.The expression changes of blood urea nitrogen(BUN),creatinine(Cr),superoxide dismutase(SOD),malondialdehyde(MDA)and nuclear factor E2-related factor 2(Nrf2)were detected individually.Results The levels of BUN,Cr and MDA gradually increased,while the level of SOD decreased in control group,ischemic group and reperfusion group (P ﹤0.05,respectively).Compared with ischemic group and reperfusion group,the levels of BUN,Cr and MDA decreased significantly,and the level of SOD increased markedly in propofol group(P ﹤0.05,respectively). The expression of Nrf2 protein and mRNA gradually increased in the control group,ischemia group,reperfu-sion group and propofol group (P ﹤0.05).Conclusion Carbon dioxide pneumoperitoneum can cause renal IRI in rats.Propofol has a protective effect of IRI by regulating the expression of Nrf2 protein.

Objective To explore the features of verbal working memory in elderly patients with anmestic mild cognitive impairment (aMCI).Methods Thirty patients with aMCI and 30 normal controls (NC), whose age were over 65 years old, were examined with verbal working memory software and the other neuropsychological tests, such as verbal fluency test (VFT) and digit span test.Results The correct rate of visual semantic verbal working memory test was significantly lower in aMCI patients (79.83% ± 3.22% )than in NC ( 87.00% ±: 1.93 % , t = - 1.03, P = 0.002).There was no significant difference of correct rate of the visual phonological verbal working memory test between the aMCI (78.92% ± 8.60% ) and NC (86.80% ±2.14%, t = - 2.34, P = 0.060).Also, aMCI cases achieved significantly lower scores than NC on VFT (22.96 ±2.31 vs 31.53 ±3.72, t = - 1.08, P =0.004) and BDS ( 1.53 ±0.86 vs 3.63 ±0.56, t = - 1.23, P = 0.027).Conclusion The visual semantic verbal working memory is impaired and visual phonetic working memory is saved in aMCI patients.Both scores of verbal fluency test and digit span test are significantly decreased in aMCI patients, too.

Objective To compare the ability of intentional forgetting to emotional words of post traumatic stress disorder(PTSD) children and normal children by item method after Wenchuan earthquake 18 months.Methods Twenty PTSD children who met with CCMD-3 and DSM-Ⅳ criteria and twenty control group children were investigated. Sixteen positive and sixteen negative words were adopted as materials. PTSD children accomplished the words learning, arithmetical operation and memory tests ( include free recall, words completion and words recognition) in orderly. Results ( 1 )The free recall accuracy of PTSD children to positive words was significant high than negative words( (0.32 ±0.11 ), (0.28 ±0.10), P＜0.05). The free recall accuracy of control group to positive words was significant high than negative words ( (0.37 ± 0.09 ), (0.34 ± 0.13 ), P＜ 0.05 ). ( 2 )The words completion accuracy of PTSD children to positive words was significant low than negative words( (0.38± 0.12 ), (0.49 ± 0.12 ), P ＜ 0.05 ). The words completion accuracy of control group to positive words was significant low than negative words ( (0.39 ± 0.15 ), (0.56 ± 0.14 ), P ＜ 0.01 ). ( 3 ) There was no difference of words recognition accuracy of PTSD children to positive and negative words ( ( 0.64 ± 0. 19 ), (0.63 ± 0. 16), P ＞0. 05). There was no difference of words recognition accuracy of control group to positive and negative words ((0.64 ± 0. 16), (0.65 ±0. 19), P ＞ 0. 05). Conclusion There is a significant intentional forgetting effect on emotional words in PTSD children and normal children, and this effect were influenced not only by selective rehearsal but also retrieval inhibition.