Human DAB2 interaction protein (DAB2IP) is a novel member of Ras GTPase-activating protein family. It interacts directly with disabled-2 protein (DAB2/DOC2) which suppresses growth of cancers derived from different tissues, including mammary, prostate and ovarian cancers. DAB2IP was identified as an immediate downstream effector mediated by DAB2/DOC2. DAB2IP and DAB2/DOC2 form a unique protein complex that has a negative regulatory effect on the Ras-mediated signal pathway. It is demonstrated that DAB2IP is a tumor suppressor gene inactivated by methylation in several cancers. This article reviews the structure and biological functions of DAB2IP gene as well as its potential roles in carcinogenesis and evolution.

Objective To investigate the expressions of enhancer of zeste(EZH2) in carcinoma of esophagus and its potential significance. Methods The expression and distribution of EZH2 were determined on the esophageal squamous carcinoma,adenocarcinoma by using immunohistochemistry.We then detected EZH2 protein expression in the esophageal squamous carcinoma, paracarcinomatous and normal tissues by Western blotting.Results The immunohistochemical staining showed EZH2 protein was highly expressed in esophageal squamous carcinoma and adenocarcinoma tissues.The positive staining was observed in the nuclear region.Normal tissues and corresponding paracarcinomatous tissues were stained weakly.Western blotting analysis showed that the strongest positive signals were detected in the esophageal squamous carcinoma(P

Objective To investigate the clinical value of uterine artery embolization on the treatment of cesarean scar pregnancy(CSP).Methods Clinical data of 112 cases of CSP patient underwent uterine artery embolization in Ningbo Women and Children's Hospital from January to December 2012 were enrolled for retrospectively analysis.Results All 112 patients underwent transvaginal ultrasound examination before admitted to hospital and among them 101 cases were diagnosed as CSP and 11 cases were suspected of CSP.Ninety-four cases were checked by magnetic resonance imaging (MRI) to measure the muscular thickness of uterine scar and the size of pregnant bursa.All patients received bilateral uterine artery embolization successfully,50-100 mg Methotrexat (MTX) were injected into uterine artery during this procedure.After uterine artery embolization,95 cases received curettage under ultrasound guidance,while 17 cases received curettage under hysteroscopy.All patients recovered as schedule.During the follow-up,no serious complication was found.Conclusion The use of uterine artery embolization in the treatment of CSP is safe and effective,and it can preserve patients' fertile ability.It is worthy recommended in the clinical application.

Objective To explore the incidence and predisposing factors of coronary heart disease (CHD) among the patients with different types and extent of calcified valvular heart disease (CVHD). Methods 574 patients with CVHD diagnosed by echocardiography were divided into mild and severe groups based on the hemodynamic changes. The differences of incidence and predisposing factors of coronary heart disease between the two groups were compared. Results Ninety-seven cases of CVHD were categorized as having severe lesion, including aortic stenosis subgroup (AS group), aortic insufficiency subgroup (AI group) and mitral valve subgroup (MV group). All other patients were having mild lesions. There was no significant difference in blood pressure, blood lipid, fasting blood glucose, age and incidence of CAD, incidences in hypertension, hyperlipidemia and diabetes mellitus between two groups. Conclusions Mild CVHD showed similar prognostic value as that of severe CVHD, therefore it should not be ignored.

Objective To study the characteristics of calcific valvular heart disease (CVHD) with the aid of echocardiography. Methods Nine thousand and seventeen patients over 50 year-old who had received transthoracic echocardiographic examination were enrolled in the study. Diagnosis of CVHD was made with 2 dimension, M-mode and color Doppler echocardiography. General state of health, clinical manifestation and history of CVHD patients were also analyzed. Results Five hundred and seventy-four cases of CVHD were diagnosed and the characteristics of CVHD were observed. The diagnostic rate increased as the age increased. There was no significant difference between male and female. The calcified regions were commoner in aortic valves than in mitral valves, more frequent in non-coronary valves than in right or left coronary valves, and more frequently seen at tips of aortic valves and annuli of mitral valves than in other parts. The size of calcific plaques was usually between 3 and 10mm. Hemodynamic changes could be found in some patients. History of hypertension, coronary artery disease, and arteriosclerosis of the aorta were often found in these patients. Conclusions Echocardiography can be used to evaluate the position of calcification, size of calcification area, and degree of valvular stenosis or insufficiency, thus offers useful information for early diagnosis and treatment, and prognosis of CVHD in clinic.

Objective To investigate the pollution status of algae and microcystin(MC)in Dianshan Lake,Shanghai City and Poyang Lake,Jiangxi Province.Methods Water samples were collected from different sites of the above lakes,as the pretreatment of intracellular and extracellular microcystins was finished,MC-LR concentration was detected by ELISA.Results The proportion of Cyanobacteria in Dianshan Lake was 38.8% and 68.2% respectively,the mean content of MC was 281.78 and 1 145.77 pg/ml respectively.In Poyang Lake,the proportion of Cyanobacteria was 48.9% and 73.7% respectively,the mean content of MC was 80.53 and 405.65 pg/ml respectively.In the same water-blooming lake,MC content was significantly lower in July than that in October(P

OBJECTIVE To investigate the reasons of pediatric nosocomial infection,analyze the possible risk factors,and provide the effective control and prevention strategies. METHODS A total of 18002 patients were investigated in 2007 by prospective study. The data were analyzed via SPSS 11.5 statistical analysis software using mainly descriptive and contrast analysis. RESULTS The nosocomial infection rate was 2.44%. The children were relatively highly infected on both of age from 1-month-old to 1-year-old (3.67%) and stay in hospital over 10 days (7.85%) ,the season in third and fourth quarters (3.13% and 2.64%). The nosocomial infection occurred mainly in respiratory tract and gastrointestinal tract,and more respiratory infections occurred in winter and spring,but more gastrointestinal tract infections occurred in summer and autumn. CONCLUSIONS Pediatric patients are more easy infected of the baby age or the longer stay in the hospital. Pediatric nosocomial infection more occurrs in the summer and autumn. Hospitals should focus on preventing and controlling the respiratory and gastrointestinal infections.

Objective To select the optimum conditions of the vacuum belt drying process of Radix Salvia Miltiorrhiza(RSM) extract.Methods The process was studied by using orthogonal test design and grading method for multi-index on the parameters of the water content of dried product and drying rate of RSM extract,the average quantity of vapour during unit time span,as the index.Results The optimum process determined by the grading method was listed as follows: water content of the extract before drying was 40%,the feeding speed was 1.5 mL/s,the belt speed was 5 cm/min.Conclusion This technology can increase the average quantity of vapour during unit time span and the drying product has high quality with lower water content and desirable drying rate.

Objective To establish an HPLC method for the determination of ginsenosides Rg1 and Re in Shenqi Granula.Methods Chromasil C18 column(250 mm?4.6 mm)was used with acetonitrile-0.05% phosphoric acid solution(21∶79)as mobile phase.The flow rate was 1 mL/min and the detected wavelength was 203 nm.Results Ginsenosides Rg1 and Re could be baseline separated with in 30 min.The average recovery rates were 99.60% and 98.5%,corresponding RSD were 1.93% and 2.31% for ginsenoside Rg1 and Re,respectively(n=5).Conclusion This method is fast and accurate and can be used for quality control of Shenqi Granula.

Objective To evaluate the effect of deep and moderate neuromuscular blockade on surgical conditions during minor laparoscopic gynecologic surgery.Methods Sixty-five patients,with expected surgery time ＜ 3 h,aged 18-60 yr,with body mass index＜30 kg/m2,of American Society of Anesthesiologists physical status Ⅰ orⅡ,scheduled for elective laparoscopic gynecological surgery,were allocated into deep neuromuscular blockade group (group D,n =33) and moderate neuromuscular blockade group (group M,n=32) using a random number table.After induction of anesthesia,the patients were tracheally intubated and mechanically ventilated.Cisatracurium was continuously infused to maintain the degree of neuromuscular blockade in both groups to achieve the target degree post-tetanic count of 1 or 2 in group D and train-of-four (TOF) count of 1 or 2 in group M.Surgical conditions were assessed and scored after surgery.The recovery index,time for TOF ratio returning to 0.7 and 0.9,surgery time,mean intra-abdominal pressure,extubation time and TOF ratio at extubation were recorded.Results Compared with group M,the mean intra-abdominal pressure was significantly decreased,and the extubation time and time for TOF ratio returning to 0.7 and 0.9 were prolonged in group D (P＜0.05).There was no significant difference in the other parameters between the two groups (P＞0.05).Conclusion Moderate neuromuscular blockade can provide better surgical conditions for minor laparoscopic gynecological surgery with shorter recovery time.