Objective To explore the effect of ceftriaxone combined with rifampicin in the treatment of children with purulent meningitis,thus to provide reference for related studies.Methods 120 children with purulent meningitis were selected and randomly divided into observation group(67 cases) and control group (53 cases) according to the digital table.The control group received treatment of ceftriaxone,and the observation group received ceftriaxone and rifampicin.The clinical effect,recovery time of clinical indicators,total protein (TP),C-reactive protein (CRP),and serum protein C level were observed in the two groups.Results After treatment,the total effective rate of the observation group was 97.01%,which was higher than 86.79% of the control group,the difference was statistically significant(x2=4.457,P<0.05).The recovery time of body temperature,peripheral leukocyte count,and CSF leukocyte in the observation group were (2.35±0.68)d,(5.27±1.56)d,(13.27±1.71)d,respectively,which in the control group were shorter than (3.36±0.36)d,(7.24±2.09)d,(16.74±2.66)d,respectively,the differences were statistically significant(t=-9.713,-5.926,-8.643,all P<0.05).After treatment,the TP and CRP decreased in two groups,serum protein C was increased,and the above indicators of the observation group improved more significantly,there were statistically significant differences(all P<0.05).There was no significant differences in the incidence of complications between the two groups(P>0.05).Conclusion Ceftriaxone combined with rifampicin in the treatment of children with purulent meningitis can reduce inflammation and promote the recovery time of clinical indicators,and it's worthy of reference.

Objective:To explore the efficacy of gamma globulin at different doses combined with dexamethasone in the treatment of children with severe idiopathic thrombocytopenic purpura. Methods: Totally 96 cases of patients with idiopathic thrombocytopenic purpura were divided into group A ( standard high dose of gamma globulin+dexamethasone) , group B ( middle dose of gamma globulin+dexamethasone) and the control group ( dexamethasone) . The clinical effects and platelet parameters of the three groups were com-pared. Results:The levels of PLT, PCT and PDW in group A and group B after the treatment were higher than those in the control group(P<0. 05). The recovery time of normal platelet and the stop time of bleeding in group A and group B were lower than those in the control group(P<0. 05), and there was no significant difference between group A and group B(P>0. 05). The clinical effective rate of group A and group B was higher than that in the control group(P<0. 05), and there was no significant difference between group A and group B(P>0.05). There was no significant difference in the incidence of adverse reactions among the three groups (P>0. 05). Conclusion:Gamma globulin at middle dose shows significant effect as at high dose in the patients with ITP, which can reduce the dosage and is worthy of clinical promotion.

Objective To observe the effect of acupuncture, neuromuscular electrical stimulation and swallowing training on acute stroke patients with dysphagia and the incidence of post-stroke pneumonia (PSP). Methods Acute stroke patients with dysphagia were divided into 3 groups. Routine group (n=188) received routine swallowing training. Stimulation group (n=196) received routine swallowing training and neuromuscular electrical stimulation. Acupuncture group (n=112) received routine swallowing training, neuromuscular electrical stimulation, and acupuncture. The incidence of improvment of dysphagia and PSP, and the improvement of National Institutes of Health Stroke Scale (NIHSS) within 3 weeks were compared. They were followed up for 3 months for the incidence of PSP. Results The incidence of improvment of dysphagia was the most in the acupuncture group (P<0.05), while the incidence of PSP was the least (P<0.05). The improvement of NIHSS was the most in the acupuncture group (P<0.05). The incidence of PSP was not significantly different (P>0.05) within 3 months followed up. Conclusion The acupuncture, neuromuscular electrical stimulation and swallowing training combination can improve the swallowing function and decrease the incidence of PSP in stroke patients with dysphagia.

A method was developed for the determination of N-acetyl-S-( N-methylcarbamoyl ) cysteine ( AMCC) in human urine by online solid-phase extraction ( SPE )-high performance liquid chromatography ( HPLC ) . The separation of AMCC from the urine matrix was performed on AmoniPac PA Solid phase Extraction ( SPE ) column with 5 mmol/L KH2 PO4 as the mobile phase by left pump. Then the time was controlled to switch the valve to make only the section of sample containing AMCC transferred into the analytic column-Acclaim PAⅡ C18 . The determination was performed using gradient elution of 0. 1% H3 PO4 (containing 5% acetonitrile) and acetonitrile by right pump. The results showed that AMCC present good linear correlation in the range of 1 . 0-100 mg/L with a correlation coefficient of above 0 . 999 , the quantitation limit of the method was 0. 2 mg/L (with the sample inject volume =10 μL), the recoveries of spiked samples were in the range of 82 . 9%-85 . 9%, and the relative standard deviation ( n=6 ) of retention time and peak area were 0. 2% and 4. 0% respectively. Compared with offline SPE-HPLC, the proposed method was convenient, environmentally friendly, efficient and stable, and feasible for the detection of AMCC in 7 human urine samples.

Objective: To establish a method for rapid determination of 47 volatile organic compounds in the air of workplace using portable gas chromatography-mass spectrometer(GC-MS). Methods: The mixed standard gas with different concentration levels was made by using the static gas distribution method with the high purity nitrogen as dilution gas. The samples were injected into the GC-MS by a hand-held probe. Retention time and characteristic ion were used for qualitative analysis,and the internal standard method was usd for quantitation. Results: The 47 poisonous substances were separated and determined well. The linear range of this method was 0.2-16.0 mg/m³,and the relative standard deviation of 45 volatile ovganic compounds was 3.8%-15.8%. The average recovery was 79.3%-119.0%. Conclusion The method is simple,accurate,sensitive,has good separation effect,short analysis period, can be used for qualitative and quantitative analysis of volatile organic compounds in the workplace, and also supports the rapid identification and detection of occupational hazards.