1.Changes of Vascular Endothelial Growth Factor in Serum and Urine in Children with Steroid-Sensitive Nephrotic Syndrome
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To investigate the changs of serum and urine vascular endothelial growth factor(VEGF) in children with steroid-sensitive nephrotic syndrome(SSNS) at their active and remission stage.Methods Serum and morning urine levels of VEGF were assayed by Liquichip in 30 patients with SSNS at the active and remission stage and 30 normal age-and sex-matched controls.Results Serum levels of VEGF in patients at the active stage [(186.62?106.21) ng/L] were significantly higher those that at the remission stage [(118.75?73.08) ng/L] and than those in control group [(108.64?54.75) ng/L](P0.05).Morning urine levels of VEGF in patients at the active stage [(201.66?100.46) ng/L] were significantly higher than those at the remission stage [(116.35?55.99) ng/L] and than those in control group [(99.94?42.07) ng/L](P0.05).Conclusions Serum and morning urine levels of VEGF are significantly elevated in patients with SSNS,however they are significantly lower after the treatment of steroid.There is no obvious difference between the patients at the remission stage and healthy controls.VEGF involves pathogenetically and physiologically in SSNS during its onset and growth.
2.Clinic trial of stellate ganglion interruption combined with Xuefu Zhuyu Capsules in the residual post-herpetic neuralgia
Hai LIN ; Xiaoyan GAO ; Junlu WANG ; Shouchun LIN
Chinese Traditional Patent Medicine 1992;0(09):-
AIM: To observe the effect of stellate ganglion interruption combined with Xuefu Zhuyu Capsules on the residual post-herpetic neuralgia (PHN) in the old. METHODS: 74 aged patients (55-87 years) suffering from residual PHN were selected and divided into two groups. and received stellate ganglion interruption (control group) and received stellate ganglion interruption combined with Xuefu Zhuyu Capsules (treatment group). The clinic effect was evaluated before and after treatment by visual analogue scale (VAS) and quality of sleep (QS). RESULTS: VAS and QS were 8.84?0.66 and 3.78?0.31, respectively, before treatment and there was significant reduction in VAS and QS after treatment in treatment group. CONCLUSION: The clinic effect of stellate ganglion interruption combined with Xuefu Zhuyu Capsules is efficient on relief from PHN and improvement of sleep quality.
3.Prevention of aseptic loosening prothesis of alendronate in beagle:abiomechanical study
Hai MENG ; Songhua YAN ; Lin YANG ; Ai GAO
International Journal of Surgery 2015;42(5):314-316,封3
Objective To investigate effect of alendronate on prevention of aseptic loosening prothesis.Method Twelve beagles which had been implanted a titanium plug in femur were divided into two groups randomly,one received alendronate,and the other placebo as control.After 12 weeks,all beagles were sacrificed.specimens of femur were studied by biomechanical test.Independent sample t test was used for statistical analyses.Results The pull-out strength of the experimental group was higher than that of the control (P < 0.05),and the torsion strength was also higher in the experimental group(P < 0.05).Conclusion The therapy of alendronate can change the bone strength,inhibit aseptic loosening of prosthesis in beagle and could be a good drug for the prevention and treatment of aseptic loosening prothesis.
4.Analysis on Medication Rule for 235 Formulae for Depression
Zuxiao BAO ; Qing TIAN ; Xinyan GAO ; Hai LIN
Journal of Zhejiang Chinese Medical University 2006;0(05):-
[Objective] To analyse medication rule for depression,and explore the mechanism and treatment features.[Method] On the basis of dealing 235 formulae with net technical,make analysis on drugs type,use frequency,nature and compatibility.[Result] The drugs can be divided into disease(1)and sign(2)differentiation medications,most drugs of 2 are Qi--and Yang-notifying;the natures are mainly sweet,bitter and warm,belonging to 5 organs.Notifying Qi and Yang,soothing the nerves are the main therapy for depression.[Conclusion] Depression belongs to deficient cold disease,deficient Yang Qi;not being able to rise is the mechanism;notifying Qi and warming Yang is the key basis for it.
6.Disposal of middle turbinate in nasal sinus endoscope operation.
Hong-Lin ZHANG ; Chong-Xue CHEN ; Yun-Hai GAO
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(5):384-385
Adolescent
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Adult
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Aged
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Endoscopy
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Female
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Humans
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Middle Aged
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Otorhinolaryngologic Surgical Procedures
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methods
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Paranasal Sinuses
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surgery
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Turbinates
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surgery
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Young Adult
7.Detection of p53 gene mutation in serum of patients with lung cancer and its clinical value.
Wei-shuo LIU ; Si-hai GAO ; Min WANG ; Qi-lin AO
Chinese Journal of Pathology 2008;37(6):406-407
Adult
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Aged
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Female
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Humans
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Lung Neoplasms
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blood
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genetics
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Male
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Middle Aged
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Mutation
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Tumor Suppressor Protein p53
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blood
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genetics
8.Sensitivity and efficacy of intraoperative electromyography monitoring in combination with 3D imaging modality during complex lumbosacral procedures
Feng XU ; Xing YU ; Lianyong BI ; Fei GAO ; Hai KE ; Lin XU
Chinese Journal of Tissue Engineering Research 2009;13(39):7613-7618
MAIN OUTCOME MEASURES: Free running EMG and stimulus triggered EMG, including time, frequency, amplitude, muscle group were observed and recorded simultaneously. Never root functional injury and restoration after surgery were detected.RESULTS: 378 pedicle screws in 74 patients were monitored intraoperatively, and only 3 pedicle screw malposition (2 of L_4, 1 of L_5) was detected and then replaced with the help of C-arm fluoroscopic examination. Myoelectricity appeared when the current intensity was less than 10 mA. The correct rate of implantation was 99.2%. Nerve root impingement was found in two cases during laminectomy for L_5 and S_1 decompression and never root solution, which alerted the surgical team of critical neural structures. Nerve symptoms of the lower limb were aggravated after surgery and restored following 2-4 weeks of conventional treatment. The error injury rate of nerve root was 2.7%. In all reported cases, no irreversible neurological deficit was observed 2-4 weeks after operation.CONCLUSION: Intraoperative EMG monitoring can find improperly placed screws and detect impending nerve root injury promptly. Combined EMG and 3-D imaging modality monitoring is a reliable and practicable method that can be used to protect neural structures during complex lumbosacral surgery.
9.Clinical Application of Immune-related Response Criteria in Evaluating Chinese Medical Treatme for Advanced Non-small Cell Lung Cancer.
Hai-wei JIANG ; Qing HU ; Dan-feng HE ; Chang GAO ; Yan-hong YAN ; Lin-tong GE
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(9):1074-1077
OBJECTIVETo evaluate the applicability of immune-related response criteria (irRC) in treating non-small cell lung cancer (NSCLC) by Chinese medicine (CM).
METHODSTotally 97 stage III a-IV NSCLC patients were predominantly treated with comprehensive CM. Curative effects were evaluated by three methods such as Response Evaluation Criteria in Solid Tumors (RECIST), Oncologic Curative Effect Evaluation Criteria of Chinese Medicine in Solid Tumor (draft, abbreviated as CM criteria), and irRC. The correspondency and consistency between irRC, RECIST and CM criteria were analyzed and compared. The objectivity of irRC in evaluating curative effect of Chinese medical treatment for NSCLC was assessed.
RESULTSThe correspondency rate of irRC to RECIST was 59. 79% with Kappa value of 0. 379 (U test, P <0. 01). The two criteria had certain correspondence, but with an unsatisfactory consistency. The correspondency rate of irRC to CM criteria rate was 83. 51% with Kappa value of 0.751 (U test, P <0. 01). The two criteria had good correspondence and consistency.
CONCLUSIONSCM criteria had good consistency with CM criteria in evaluating curative effect for Chinese medical treatment of advanced NSCLC. Its results could objectively reflect features and advantages of CM for treating advanced NSCLC.
Asian Continental Ancestry Group ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; immunology ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Lung Neoplasms ; drug therapy ; immunology ; Medicine, Chinese Traditional ; standards ; Treatment Outcome
10.Direct inhibitory effects of 153Sm-DTPA-c(CGRRAGGSC) on human prostate cancer PC-3 cells
Qing-hua, WU ; Lu, LIU ; Ze-xuan, YANG ; Hai-lin, GAO ; Jin, SUN ; Qi, NIE
Chinese Journal of Nuclear Medicine 2011;31(4):241-244
Objective To investigate the direct inhibitory effects of 153Sm- DTPA-c (Cys-Gly-Arg-Arg-Ala-Gly-Gly-Ser-Cys) NH2 ( 153 Sm-DTPA-c (CGRRAGGSC)) on human prostate cancer PC-3 cells. Methods 153Sm-DTPA-c(CGRRAGGSC) was synthesized by the reaction of 153SmCl3 with DTPA-c(CGRRAGGSC) using indirect synthesis method. PC-3 cells in vitro culture were divided into four study groups, groug A ( the control, with PBS only), group B with 1.5 mg/L c ( CGRRAGGSC), group C with 370 kBq 153 SmCl3 and group D with 370 kBq 153Sm-DTPA-c(CGRRAGGSC). PC-3 cell growth was assayed by 3-(4, 5-dimethylthiazol-2-yl ) -2, 5-diphenyltetrazolium bromide (MTT) method. Cell cycle and apoptosis were analyzed by flow cytometry. The expression changes of interleukin 11 (IL11 ) and IL11 receptor (IL1 1 R) in PC-3 cells were examined by Western Blot. One way analysis of variance (ANOVA) and paired-t test were applied for statistic analysis. Results The labeling yield of 153Sm-DTPA-c (CGRRAGGSC) was 85% and the radiochemical purity was 95.8%. The specific activity of 153Sm-DTPA-c(CGRRAGGSC) was 1.32 × 105 MBq/μmol. Significant inhibitory effects on the growth of PC-3 cells were found in both group C and D, with a time-dependent manner. However, no obvious inhibition was found either in group A or in group B. After 48 h,significant differences of sub-G1 peak area were found among groups, (0. 98 ± 0. 18)%, (0. 35 ±0. 10)%, (4.05 ±0.28)% and (13.38 ±0. 89)% for group A, B, C and D, respectively. Furthermore,sexpression of ILl 1R in group D was significantly lower than that in group B and C with absorbance values 0. 339 ~ 0.014, 0.338 ~ 0.019, 0.226 ~ 0. 015 for group B, C and D, respectively. Absorbance values in groups B and C were not significantly different after treatment, compared with those before treatment; however, there was difference between absorbance values after and before treatment in group D ( t = 0. 405,1. 163,135.989,P>0.05 >0.05, <0.05). Conchluion 153Sm-DTPA-c(CGRRAGGSC) can directly in hibit the cell growth and expression of human prostate cancer cells PC-3.